Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 243 C→T 5.6% N18N (AAC→AAT thrL → thr operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132430CT 5.6% 400.3 / 7.8 125N18N (AAC→AATthrLthr operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (75/43);  new base T (4/3);  total (79/46)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

Only 100 of 127 total aligned reads displayed.

AGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCG  >  NC_000913/94‑392
                                                                                                                                                     |                                                                                                                                                       
aGTAAATTAAAATTTCATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAAc                                                                                                                                                         >  2:629657/1‑150 (MQ=255)
  tAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACgg                                                                                                                                                       <  2:1320402/150‑1 (MQ=255)
  tAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACACATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGCTAACgg                                                                                                                                                       >  2:1729160/1‑150 (MQ=255)
    aaTTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTg                                                                                                                                                     <  1:1407657/150‑1 (MQ=255)
     aTTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCTGTGGTAATGGTga                                                                                                                                                    <  1:1028031/150‑2 (MQ=255)
         aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATTGCGCACAGACAGATAAAAATTACAGAGTACACAACAACCATGTAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggt                                                                                                                                                <  2:104628/150‑6 (MQ=255)
         aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTCCCACAGGTAACGGTGCGGGc                                                                                                                                                <  1:1993909/150‑1 (MQ=255)
          gtatttAGTGCCCTAAGTCAATAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCt                                                                                                                                               >  1:1472146/4‑150 (MQ=255)
           aTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTg                                                                                                                                              >  1:632373/1‑150 (MQ=255)
              ttATTGACTTAGGTCACTAAATACTTTATCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcg                                                                                                                                           <  2:1369790/150‑1 (MQ=255)
                aTTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcgcg                                                                                                                                         >  1:34095/1‑150 (MQ=255)
                    aCTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACa                                                                                                                                     <  1:928798/150‑1 (MQ=255)
                     cTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAg                                                                                                                                    >  1:1289116/1‑150 (MQ=255)
                          gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                               <  1:1108579/150‑1 (MQ=255)
                          gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                               <  2:1173387/150‑1 (MQ=255)
                           tCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAaca                                                                                                                              >  2:712170/1‑150 (MQ=255)
                            cACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAacac                                                                                                                             >  2:1437508/1‑150 (MQ=255)
                            cACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAacac                                                                                                                             >  2:503349/1‑150 (MQ=255)
                                 aaTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGaaa                                                                                                                        <  2:929758/150‑1 (MQ=255)
                                     cTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACAACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAg                                                                                                                    >  1:1296911/1‑150 (MQ=255)
                                       ttAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGcc                                                                                                                  >  1:1336704/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAATGCCCGc                                                                                                               >  2:693107/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                               >  2:1373119/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                               >  2:1463295/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                               >  1:389417/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACAACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                               >  2:894142/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGAATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGTAAAAAGCCCGc                                                                                                               >  2:524535/1‑150 (MQ=255)
                                                 taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACAACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGAc                                                                                                        >  2:276055/1‑150 (MQ=255)
                                                  aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa                                                                                                       >  2:1758409/1‑150 (MQ=255)
                                                    gCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCAGTAGCACCCCCATTACCACCACCATCACCATTACCACAGGTAACGGAGCGGGCTGCCGCGTACAGGACACACAGAAAAAAGCCCGCCccagccagg                                                                                                    >  1:394141/1‑149 (MQ=255)
                                                        aGCGCACAGACTGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTggcg                                                                                                >  2:911939/1‑147 (MQ=255)
                                                        aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCgg                                                                                                >  1:6213/1‑150 (MQ=255)
                                                         gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCggg                                                                                               <  2:1209034/150‑1 (MQ=255)
                                                          cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTCCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAACAGCCCGCACCTGACAGTGCGGGc                                                                                              <  1:546117/150‑1 (MQ=255)
                                                           gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATAAGAACGACCATTACCACCACCATCACCATTAGCACAGGTAACGGTGCGGGCTTACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                            >  2:680629/1‑150 (MQ=255)
                                                              cagacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttt                                                                                         >  2:1982060/1‑150 (MQ=255)
                                                                gacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttt                                                                                       >  2:1477091/1‑150 (MQ=255)
                                                                  cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGTTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                     <  2:40443/150‑1 (MQ=255)
                                                                  aagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                     <  2:1827759/149‑1 (MQ=255)
                                                                    gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttctgt                                                                                   <  1:1300974/150‑5 (MQ=38)
                                                                     aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttttttc                                                                                  <  1:673779/150‑3 (MQ=255)
                                                                       ataaaTTACAGAGTACACTACATCCATGAAACGCATTAGCACCACCATTACCACCTCCATCTCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAACCACAGAAAACAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAc                                                                                <  2:375420/148‑1 (MQ=255)
                                                                        aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAcc                                                                               >  1:1639548/1‑150 (MQ=255)
                                                                        aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGAGCGGGCAATTTTTTACGAcc                                                                               >  1:155177/1‑150 (MQ=255)
                                                                         aaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCTGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCa                                                                              >  1:242959/1‑150 (MQ=255)
                                                                          aaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaa                                                                             <  1:672966/150‑1 (MQ=255)
                                                                          aaTTACAGAGTACACAACATCCAGGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaa                                                                             <  1:201592/150‑1 (MQ=255)
                                                                           aTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCCCCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaaa                                                                            <  1:939270/150‑1 (MQ=255)
                                                                             tACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAgg                                                                          <  2:1172375/150‑1 (MQ=255)
                                                                                agcgTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTCCGGGCTTTTTTTTTCGACCAAAGGTaa                                                                       >  2:625572/4‑150 (MQ=255)
                                                                                agagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGCCCAAAGGTaa                                                                       >  2:1708209/1‑150 (MQ=255)
                                                                                 cagaaaacaacaTCCATGAAACGCATTACCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAAc                                                                      <  1:1752826/144‑1 (MQ=255)
                                                                                     acacAACATCCATGAAACGCATTCGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAgg                                                                  <  1:2011585/150‑1 (MQ=255)
                                                                                     acacAACATCCATGAAACGCATTCGCACCACCATTACCACCACCATCACCATAACCACAGGTAACGGTGCGGGCGGACGCGTACAGGAAACACAGAAAAACGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAgg                                                                  <  1:510222/150‑1 (MQ=255)
                                                                                     acacAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAgg                                                                  <  1:1554539/150‑1 (MQ=255)
                                                                                      cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGTGCTTTTTTTTTCGACCAAAGGTAACGACGt                                                                 >  1:631730/1‑150 (MQ=255)
                                                                                      cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGt                                                                 <  1:1437508/150‑1 (MQ=255)
                                                                                      cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGt                                                                 >  2:859199/1‑150 (MQ=255)
                                                                                      cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGt                                                                 >  1:1055564/1‑150 (MQ=255)
                                                                                           caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaa                                                            >  2:399103/1‑150 (MQ=255)
                                                                                            aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaac                                                           >  2:642865/1‑150 (MQ=255)
                                                                                              ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                         >  1:698304/1‑150 (MQ=255)
                                                                                                   aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa                                                    >  2:862019/1‑150 (MQ=255)
                                                                                                    aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg                                                   >  1:1344337/1‑150 (MQ=255)
                                                                                                     aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt                                                  <  1:232044/150‑1 (MQ=255)
                                                                                                      cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCCCCTGACAGTGCGGGCTTTATTTTTAGACCAAAAGTAACGAGGTAACAACCAAGCGactt                                                 >  1:969597/1‑147 (MQ=255)
                                                                                                      cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtg                                                 <  2:1146205/150‑1 (MQ=255)
                                                                                                             gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCCCCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGt                                          >  2:423148/1‑150 (MQ=255)
                                                                                                              caccacCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAGTGTTGAAGtt                                         >  2:1680777/1‑150 (MQ=255)
                                                                                                               accaccATTACCACCACCATAACCATTACCACAGGTAACGGTGCGGGATGACGCGTACAGGCAACACATAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                                        <  1:1823926/150‑1 (MQ=255)
                                                                                                                 caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                      >  2:1623205/1‑150 (MQ=255)
                                                                                                                 caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                      >  1:1204439/1‑150 (MQ=255)
                                                                                                                  accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                     <  2:269081/150‑1 (MQ=255)
                                                                                                                  accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                     >  2:1561131/1‑150 (MQ=255)
                                                                                                                       tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc                                <  2:632373/150‑1 (MQ=255)
                                                                                                                        accaccaccATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa                               >  1:1538310/1‑150 (MQ=255)
                                                                                                                         ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa                                            <  1:495257/136‑1 (MQ=255)
                                                                                                                         ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa                                            >  2:495257/1‑136 (MQ=255)
                                                                                                                         ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAATGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt                              >  1:792870/1‑150 (MQ=255)
                                                                                                                           accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGt                                  <  2:1877408/144‑1 (MQ=255)
                                                                                                                           accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGt                                  >  1:1877408/1‑144 (MQ=255)
                                                                                                                              accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGCCCAAAGGTAACGAGGTACCAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg                         >  2:982533/1‑150 (MQ=255)
                                                                                                                              accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg                         <  2:1199853/150‑1 (MQ=255)
                                                                                                                              accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg                         <  1:1819911/150‑1 (MQ=255)
                                                                                                                               ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTTACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg                        >  1:402029/1‑150 (MQ=255)
                                                                                                                               ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg                        >  1:518034/1‑150 (MQ=255)
                                                                                                                                ccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc                       >  1:1304390/4‑150 (MQ=255)
                                                                                                                                catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGGGTTGAAGTTCGGCGGTACATCAGTGGc                       >  2:515966/1‑150 (MQ=255)
                                                                                                                                 cgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa                      >  1:31068/3‑150 (MQ=255)
                                                                                                                                 cgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa                      >  1:1484349/3‑150 (MQ=255)
                                                                                                                                 atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa                      <  2:1790669/150‑1 (MQ=255)
                                                                                                                                    accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCCACCAAAGGTAACGAGGTAACCACCATTCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAAt                   >  1:55633/1‑150 (MQ=255)
                                                                                                                                          aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa             >  2:880980/1‑150 (MQ=255)
                                                                                                                                           ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc            >  2:1365108/1‑150 (MQ=255)
                                                                                                                                           ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc            >  2:1039319/1‑150 (MQ=255)
                                                                                                                                               aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt        >  2:192709/1‑150 (MQ=255)
                                                                                                                                                ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtttt       >  1:1491754/1‑150 (MQ=255)
                                                                                                                                                  tAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc      <  1:894142/150‑1 (MQ=255)
                                                                                                                                                   aaCGGTGCGGGCTGACGCGTACAGGAAACTCAGAAAAACGCCCGCACCTGACATTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg    <  2:1667031/150‑1 (MQ=255)
                                                                                                                                                     cGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCg  <  2:285446/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
AGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCG  >  NC_000913/94‑392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: