Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 282,513 T→C 7.0% L79P (CTG→CCG)  yagE → 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913282,5130TC 7.0% 440.6 / 18.7 160L79P (CTG→CCG) yagE2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base T (68/75);  new base C (4/7);  total (73/87)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-01

Only 100 of 162 total aligned reads displayed.

CGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCC  >  NC_000913/282364‑282661
                                                                                                                                                     |                                                                                                                                                    
    gcgcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATc                                                                                                                                                  <  2:849088/150‑1 (MQ=255)
     cgcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCg                                                                                                                                                 <  1:1467313/150‑1 (MQ=255)
      gcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTATGCTATCGATCATGTCGCTCGTCGCGTGCCGGTGCAGATCgg                                                                                                                                                >  2:181686/1‑150 (MQ=255)
            cgacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcgg                                                                                                                                          >  2:323827/1‑150 (MQ=255)
            cgacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcgg                                                                                                                                          >  2:1143237/1‑150 (MQ=255)
             gacgaTCTGATTAAAGCAGGCGTAGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc                                                                                                                                         <  1:449765/150‑1 (MQ=255)
             gacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc                                                                                                                                         <  2:1827046/150‑1 (MQ=255)
             gacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTCGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc                                                                                                                                         <  1:86166/150‑1 (MQ=255)
              acgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggca                                                                                                                                        <  2:1530707/150‑2 (MQ=255)
              acgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGGGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGTTCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcggcg                                                                                                                                        <  2:1251515/150‑1 (MQ=255)
               cgaTCTGATCAAAGCAGGCGGTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggcac                                                                                                                                       <  1:1219888/150‑3 (MQ=255)
                 aTCTGCTCAACGCAGGCGGTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa                                                                                                                                     <  1:1404520/150‑1 (MQ=255)
                     gATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCaa                                                                                                                                 >  1:869970/1‑150 (MQ=255)
                     gATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCaa                                                                                                                                 >  2:207574/1‑150 (MQ=255)
                     gATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCaa                                                                                                                                 >  1:491491/1‑150 (MQ=255)
                       tCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGAAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGAGCAAGTCGATCGTCGCGTTCAGGTGCTCATCTGCACCGGCTGCACCAACg                                                                                                                               >  1:1191219/1‑150 (MQ=255)
                        cAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCTAGTTCTCCCAGCTCGGCGCCGAAGAGCGAAAAGCCATTGCCCGCTTTGCAATCGATAATGTCGATCGTAGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGc                                                                                                                              >  1:1285545/1‑150 (MQ=255)
                         aaaGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGcc                                                                                                                             >  2:1331080/1‑150 (MQ=255)
                            gCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCgg                                                                                                                          <  1:482401/150‑1 (MQ=255)
                            gCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCAAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCgg                                                                                                                          <  1:1039073/150‑1 (MQ=255)
                                gCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGaaa                                                                                                                      >  1:60837/1‑150 (MQ=255)
                                gCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGaaa                                                                                                                      >  1:267938/1‑150 (MQ=255)
                                 cGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc                                                                                                                     <  1:1931579/150‑1 (MQ=255)
                                 cGTGGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc                                                                                                                     <  1:455698/150‑1 (MQ=255)
                                  gTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAcc                                                                                                                    <  1:677404/150‑1 (MQ=255)
                                   ttGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCa                                                                                                                   <  1:853485/150‑1 (MQ=255)
                                   ttGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCGTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCa                                                                                                                   <  1:1418697/150‑1 (MQ=255)
                                    tGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCACCGCCCGGGAAACCAt                                                                                                                  >  1:352185/1‑150 (MQ=255)
                                      aCGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCg                                                                                                                <  2:630301/150‑1 (MQ=255)
                                        ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAATCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGTCACCGGCGGCACCAACGCCCGGGAAACCATCGaa                                                                                                              <  1:1060115/150‑1 (MQ=255)
                                         gCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAAc                                                                                                             <  2:1920616/150‑1 (MQ=255)
                                                 ttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcagccag                                                                                                     >  2:798942/1‑150 (MQ=255)
                                                    cTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGGAACCATCGAACTCAGCCAGCAc                                                                                                  >  2:622769/1‑150 (MQ=255)
                                                       ggCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCAcgcg                                                                                               <  2:770248/150‑1 (MQ=255)
                                                        gCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACgcgc                                                                                              <  1:812428/150‑1 (MQ=255)
                                                              gTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCATCGCCCGGGACACCATCGAACTCAGCCAGCACGCGCCGCAgg                                                                                        >  1:953674/1‑150 (MQ=255)
                                                                  cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCCCCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCCGCACGCGCAGCCggcggg                                                                                    >  1:1847396/1‑150 (MQ=255)
                                                                  cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACTGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcggg                                                                                    <  1:1163630/150‑1 (MQ=255)
                                                                  cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcggg                                                                                    >  2:1299567/1‑150 (MQ=255)
                                                                      ttCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTTCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGcgcg                                                                                <  2:1794074/150‑1 (MQ=255)
                                                                           ccAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACgg                                                                           >  2:1891732/1‑150 (MQ=255)
                                                                              gCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGCTCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCAt                                                                        >  1:1385408/1‑150 (MQ=255)
                                                                                tCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCCGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCg                                                                      >  2:471281/1‑150 (MQ=255)
                                                                                tCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCg                                                                      <  2:262279/150‑1 (MQ=255)
                                                                                tCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCg                                                                      >  1:888304/1‑150 (MQ=255)
                                                                                 cGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgt                                                                     >  2:423001/1‑150 (MQ=255)
                                                                                  ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg                                                                    >  1:763067/1‑150 (MQ=255)
                                                                                  ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg                                                                    >  1:1478215/1‑150 (MQ=255)
                                                                                  ggCGCCGAAGAGCGTAAAGCCATGGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg                                                                    <  1:918096/150‑1 (MQ=255)
                                                                                    cgcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtggt                                                                  <  2:915388/150‑1 (MQ=255)
                                                                                     gcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtggtg                                                                 <  2:1349758/150‑1 (MQ=255)
                                                                                      ccGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtggtgc                                                                >  1:361983/1‑149 (MQ=255)
                                                                                      ccGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGa                                                                <  2:1578166/150‑1 (MQ=255)
                                                                                          agagCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCaa                                                            >  2:1492216/1‑150 (MQ=255)
                                                                                            agCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCCTCGTGGTGATCAAcc                                                          >  1:664158/1‑150 (MQ=255)
                                                                                             gCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAAcct                                                         <  2:217670/150‑2 (MQ=255)
                                                                                             gCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAAccc                                                         <  1:297427/150‑1 (MQ=255)
                                                                                              cGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAAcccc                                                        >  1:243847/1‑150 (MQ=255)
                                                                                               gTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGACACCATCGAACTCAGCCAGCCCGCGCAGCAGGCGGGCGCGGCCGGCATCGTGGTGATCAACCCct                                                       >  1:1006231/1‑150 (MQ=255)
                                                                                               gTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCct                                                       <  2:818382/150‑1 (MQ=255)
                                                                                                 aaaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCctac                                                     <  1:1707331/150‑1 (MQ=255)
                                                                                                 aaaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCctac                                                     >  2:1742300/1‑150 (MQ=255)
                                                                                                  aaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCctact                                                    <  2:1162007/150‑1 (MQ=255)
                                                                                                   aGCCATTGCCCGCGTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCCCCCACGCCCGGGAACCCATCGAACGCCGCCAGCACGCGCAGCAGGAGGGCGCGGACGGCATCGTGGTGCTCAACCCctacta                                                   >  2:157946/1‑150 (MQ=255)
                                                                                                     ccATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTactact                                                 <  2:359857/150‑1 (MQ=255)
                                                                                                        ttGCCCGCTTTGCTATCGAGCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACGCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGTATCGTGGTGATCAACCCCTACTACTGGa                                              <  1:1017347/150‑1 (MQ=255)
                                                                                                         tGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACTGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa                                             <  2:479347/150‑1 (MQ=255)
                                                                                                         tGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa                                             <  1:32750/150‑1 (MQ=255)
                                                                                                         tGCCCGCTTTGCTATCGATCATGTCGAGCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa                                             <  1:1555678/150‑1 (MQ=255)
                                                                                                          gCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaaa                                            >  1:402647/1‑150 (MQ=255)
                                                                                                           cccGCTTTGCTATCGATCATGTCGATCGCCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCACGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAg                                           >  2:1828275/1‑150 (MQ=255)
                                                                                                            ccGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAgt                                          >  2:1413398/1‑150 (MQ=255)
                                                                                                              gCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAgtgt                                        <  2:754584/150‑1 (MQ=255)
                                                                                                               cTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTc                                       >  1:474917/1‑150 (MQ=255)
                                                                                                                tttGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCg                                      <  1:1957946/150‑1 (MQ=255)
                                                                                                                 ttGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCGGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCgg                                     <  1:1484080/150‑1 (MQ=255)
                                                                                                                    cTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAg                                  >  1:1228834/1‑150 (MQ=255)
                                                                                                                     tATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGc                                 <  2:422747/150‑1 (MQ=255)
                                                                                                                     tATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGc                                 <  2:898936/150‑1 (MQ=255)
                                                                                                                      aTCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCg                                >  1:1649485/1‑150 (MQ=255)
                                                                                                                          aTCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAAcc                            <  1:1389440/150‑1 (MQ=255)
                                                                                                                           tCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCt                           <  1:112366/150‑1 (MQ=255)
                                                                                                                           tCATGTCGATCGTCGCGTGCCGGTGCGGATCGGCACAGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCt                           <  2:392171/150‑1 (MQ=255)
                                                                                                                                 ggATCGTCGCGTGCCGGTGCGGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCg                     <  1:1751676/149‑1 (MQ=255)
                                                                                                                                       tcgCGTGCCGGTGCTGAGCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTAttt               <  2:946689/150‑1 (MQ=255)
                                                                                                                                         gcgTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCg             <  2:267938/150‑1 (MQ=255)
                                                                                                                                         gcgTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCg             >  1:342650/1‑150 (MQ=255)
                                                                                                                                            tGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGc          <  1:250348/150‑1 (MQ=255)
                                                                                                                                            tGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGc          <  2:781938/150‑1 (MQ=255)
                                                                                                                                            cgCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGc          >  1:1665901/2‑150 (MQ=255)
                                                                                                                                             gCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCa         <  1:397630/150‑1 (MQ=255)
                                                                                                                                             gCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCGACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCgcgcg         >  2:1976798/1‑149 (MQ=255)
                                                                                                                                             gCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGCGCa         >  2:281916/1‑150 (MQ=255)
                                                                                                                                             gCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCa         >  2:1636810/1‑150 (MQ=255)
                                                                                                                                               gggtggtGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAgg       <  2:1694314/149‑1 (MQ=255)
                                                                                                                                                ggTGCTGATCGGCACCGGCGGCACCAACTCCCGGGAAACCATCGAACTCAGACAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCACTATTTCGATCAGGt      <  1:77918/150‑1 (MQ=255)
                                                                                                                                                ggTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCCGCAGGCGGGCGCGGACGGCATCGTGGTGATCACCCCCTACGACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGt      >  2:1020063/1‑150 (MQ=255)
                                                                                                                                                   gCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGCTCCGCTATTTCGAGCAGGTGGc   >  2:180207/1‑150 (MQ=255)
                                                                                                                                                    cTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGCACCTGATCCGCTATTTCGAGCAGGTGGcc  >  1:2003721/1‑150 (MQ=255)
                                                                                                                                                    cTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGcc  >  2:586107/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCC  >  NC_000913/282364‑282661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: