Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,045,127 T→G 5.8% intergenic (+26/+31) bglA → / ← ygfF 6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,045,1270TG 5.8% 419.7 / 9.3 136intergenic (+26/+31)bglA/ygfF6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (54/73);  new base G (5/3);  total (60/76)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 140 total aligned reads displayed.

AAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATG  >  NC_000913/3044979‑3045275
                                                                                                                                                    |                                                                                                                                                    
aaaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACtt                                                                                                                                                     >  2:1828550/1‑150 (MQ=255)
aaaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACtt                                                                                                                                                     >  2:185618/1‑150 (MQ=255)
   cGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgtt                                                                                                                                                  <  1:1912173/150‑1 (MQ=255)
        cGGCTTTATCTATGTGAATAAACATGACGACGGTACTGTCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTTCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTATAATTCTATGCAGTACCGGATGCAATACTTGTTGCATc                                                                                                                                             >  1:1236359/1‑150 (MQ=255)
          gCTTTAGCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCg                                                                                                                                           <  1:505562/150‑1 (MQ=255)
            tttATCTATTTTAATAAACATTAAGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                         <  1:1664196/150‑1 (MQ=255)
            tttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                         <  1:2010487/150‑1 (MQ=255)
              tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                       >  2:1383459/1‑150 (MQ=255)
              tATCTATGTGAAAAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                       <  1:1545712/150‑1 (MQ=255)
               aTCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAg                                                                                                                                      <  1:15060/150‑1 (MQ=255)
               aTCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAg                                                                                                                                      <  2:916389/150‑1 (MQ=255)
                 cTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGAc                                                                                                                                    >  1:840697/1‑150 (MQ=255)
                 cTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGAc                                                                                                                                    >  2:229448/1‑150 (MQ=255)
                  tatgctaaTAAACAAGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTCACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGCTGCATCCGGTCAGACa                                                                                                                                   <  2:390698/144‑1 (MQ=255)
                  tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTCCAAAGAGGTGATTGCCAGCAACGTCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                   <  1:1340608/150‑1 (MQ=255)
                        aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAATCGATTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTcc                                                                                                                             >  1:235751/1‑150 (MQ=255)
                         aTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTccc                                                                                                                            <  1:1188832/150‑1 (MQ=255)
                          tAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCt                                                                                                                           >  2:1781533/1‑150 (MQ=255)
                            aaCATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGt                                                                                                                         >  1:1511656/1‑150 (MQ=255)
                               aTGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGtttt                                                                                                                      <  1:746204/150‑1 (MQ=255)
                               aTGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGtttt                                                                                                                      <  2:810062/150‑1 (MQ=255)
                                tGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTTGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTa                                                                                                                     >  2:1645632/1‑150 (MQ=255)
                                 gacgacGGTACTGGCGATATGTCGCGTTCACGTAAGAAGTGCTTTAACTGGTACAAAGTGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAAGACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAt                                                                                                                    <  1:1252910/150‑1 (MQ=255)
                                  acgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAtt                                                                                                                   <  2:1458526/150‑1 (MQ=255)
                                   cgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAttt                                                                                                                  <  2:1542665/150‑1 (MQ=255)
                                      cgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTccc                                                                                                               <  2:550145/150‑1 (MQ=255)
                                        gTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgc                                                                                                             <  2:1733186/150‑1 (MQ=255)
                                           cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                          <  1:923420/150‑1 (MQ=255)
                                           cTGGCGATATGTCGCGTTCACGTAAAAAGAGCTAAAACTGGTACAAAGAACTGATTGCAAGCAAAGGCGAGAACCTTTAAGTCTATAAAGTACCGGATGCAATACTTGTTGCATACGTTAATACAACTTCCCTGTTATATTTaccgccct                                                                                                          >  1:1082375/1‑149 (MQ=255)
                                             ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                        >  1:1891602/1‑150 (MQ=255)
                                             ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATAGACAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                        >  1:491155/1‑150 (MQ=255)
                                              gCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCa                                                                                                       >  2:1906630/1‑150 (MQ=255)
                                              gCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCa                                                                                                       >  2:1195401/1‑150 (MQ=255)
                                                 atatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAAt                                                                                                    <  1:185618/150‑1 (MQ=255)
                                                  tatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTTTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                   <  2:1902168/150‑1 (MQ=255)
                                                    tGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGa                                                                                                 <  2:1899859/150‑1 (MQ=255)
                                                     gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                >  1:1560719/1‑150 (MQ=255)
                                                     gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                >  1:742847/1‑150 (MQ=255)
                                                     gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGCTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                >  1:1520419/1‑150 (MQ=255)
                                                       cgcgTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATaa                                                                                              <  2:111961/150‑1 (MQ=255)
                                                       cgcgTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAAGCGATaa                                                                                              <  2:68602/150‑1 (MQ=255)
                                                          gTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGGATCGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATTCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAc                                                                                           <  2:1303110/150‑1 (MQ=255)
                                                          gTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAc                                                                                           <  1:272648/150‑1 (MQ=255)
                                                             cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTc                                                                                        >  2:1947115/1‑150 (MQ=255)
                                                              aCGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTcc                                                                                       >  2:1697376/1‑150 (MQ=255)
                                                              aCGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTcc                                                                                       >  1:52051/1‑150 (MQ=255)
                                                               cGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTccc                                                                                      <  2:907763/150‑1 (MQ=255)
                                                                  aagaagAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTg                                                                                   >  2:1062432/1‑150 (MQ=255)
                                                                       gagCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTa                                                                              <  1:314877/150‑1 (MQ=255)
                                                                          cTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAaga                                                                           <  1:1322548/150‑1 (MQ=255)
                                                                             tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                        >  2:411246/1‑150 (MQ=255)
                                                                              aaCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCt                                                                       >  2:391837/1‑150 (MQ=255)
                                                                               aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAATAGTTCCCGTGACGTAAGAGGCtt                                                                      >  1:538010/1‑150 (MQ=255)
                                                                               aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                      <  1:253078/150‑1 (MQ=255)
                                                                                 tGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTa                                                                    <  1:598198/150‑1 (MQ=255)
                                                                                   gTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATc                                                                  >  1:638699/1‑150 (MQ=255)
                                                                                     aCAAAGAGTTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAc                                                                <  1:1174489/150‑1 (MQ=255)
                                                                                     aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAc                                                                <  2:840697/150‑1 (MQ=255)
                                                                                       aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                              <  2:780275/150‑1 (MQ=255)
                                                                                        aaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTta                                                             <  1:1102285/150‑1 (MQ=255)
                                                                                         agagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtag                                                            <  2:1556984/150‑1 (MQ=255)
                                                                                         agagGGGATTGCCAGCAAAGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCTCTTTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtag                                                            <  1:163994/150‑1 (MQ=255)
                                                                                          tagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCTCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagt                                                           <  1:1804105/149‑1 (MQ=255)
                                                                                            ggTCATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGCCAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagtag                                                         >  2:1404866/1‑150 (MQ=255)
                                                                                              tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                       <  2:1986558/150‑1 (MQ=255)
                                                                                              tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAAAACATGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                       >  2:92710/1‑150 (MQ=255)
                                                                                                 ttGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATATCCCGCCCGCCAAATCGATAAAACTTCCCGTGAAGTAAGAGGCTTTATCACTTAGTAGCCAgc                                                    >  1:332317/1‑149 (MQ=255)
                                                                                                  tGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTTCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGAc                                                   <  2:1959800/150‑1 (MQ=255)
                                                                                                    ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                                 >  2:1008048/1‑150 (MQ=255)
                                                                                                    ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGAAATACTTGTTGCATCCGGACAGACAACTTCCCTGTTTTATTTCCAGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                                 >  2:1775150/1‑150 (MQ=255)
                                                                                                       gCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATgg                                              >  1:843283/1‑150 (MQ=255)
                                                                                                       gCAACGGCGAGAAGCTTATATTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCACTGTTTTATTTCCCGCCCGCCAAATCGATATAACTTCCCGTGACGTAAGATGCTTTATCACTTAGTAGCCAGACAATgg                                              <  2:1769760/150‑1 (MQ=255)
                                                                                                        cAAAGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGc                                             <  2:304540/150‑1 (MQ=255)
                                                                                                             gCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCACCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTgcg                                        <  2:417993/150‑1 (MQ=255)
                                                                                                                agaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGa                                     >  1:619323/1‑150 (MQ=255)
                                                                                                                 gaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGTCAACTTCCCTGTTTTATATCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAc                                    <  2:972336/150‑1 (MQ=255)
                                                                                                                      tttAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtctt                               >  1:425980/1‑150 (MQ=255)
                                                                                                                       ttAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCATACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAATTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttc                              <  2:1202942/150‑1 (MQ=255)
                                                                                                                        tAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttct                             <  1:229448/150‑1 (MQ=255)
                                                                                                                            tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCt                         <  1:1781533/150‑1 (MQ=255)
                                                                                                                            tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCt                         <  1:1977862/150‑1 (MQ=255)
                                                                                                                             cGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTg                        >  2:665670/1‑150 (MQ=255)
                                                                                                                             agATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGGGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTg                        <  2:1272251/149‑1 (MQ=255)
                                                                                                                              gATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGt                       >  1:630276/1‑150 (MQ=255)
                                                                                                                               aTGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACACCTTCGGTGTTTTAGTTCCCGCCCGCCATATCGATAAAACTTCCCGTGACGTAAGAGGATTTAGCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTc                      >  1:365943/1‑150 (MQ=255)
                                                                                                                                tGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcc                     <  2:444404/150‑1 (MQ=255)
                                                                                                                                 gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcca                    >  2:4611/1‑150 (MQ=255)
                                                                                                                                 gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcca                    <  1:1645632/150‑1 (MQ=255)
                                                                                                                                    tgaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGAGCGACCGCTTCTGCCTGTccacac                 >  1:124916/3‑148 (MQ=255)
                                                                                                                                     gaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGCGGTAAGCGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCcaccac                >  2:1401167/2‑150 (MQ=255)
                                                                                                                                     gaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCcaccac                >  1:791962/2‑150 (MQ=255)
                                                                                                                                        cGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAAAGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCt             >  2:1209159/1‑150 (MQ=255)
                                                                                                                                        cGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCt             >  2:1145551/1‑150 (MQ=255)
                                                                                                                                         ggATGCAATAATTGTTGCATCCGGTCAGACAACTTCCCTGATTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAAGTAGTAGCCAGACAATGCCCTGCGCGACATCTTCTGACTGTCCACCACGCTg            >  2:1465104/1‑150 (MQ=255)
                                                                                                                                         ggATGCAACAAGTATTGCATCCGGTCAGCCAACTTCCCTGTTTTATTTCACGCCCGCCAAATCGATAAAACTTCCGGTGACGTAAGAGGGTTTATCACTTAGTAGCCAGACACTGGCCTGCGCGACCTCTGCTGCCTGTCCACACCGCTg            >  2:972214/1‑150 (MQ=255)
                                                                                                                                            tGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCCCCACGCTGCAt         >  1:43517/1‑150 (MQ=255)
                                                                                                                                              cAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTAAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCAtcg       >  1:927374/1‑148 (MQ=255)
                                                                                                                                               aaTACTGGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTACGAGGCTTTATCACATAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg      <  2:1091421/150‑1 (MQ=255)
                                                                                                                                                 tACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGa    <  1:1062432/150‑1 (MQ=255)
                                                                                                                                                 tACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGa    <  1:1968118/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATG  >  NC_000913/3044979‑3045275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: