Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,045,129 G→A 5.9% intergenic (+28/+29) bglA → / ← ygfF 6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,045,1290GA 5.9% 423.5 / 10.1 136intergenic (+28/+29)bglA/ygfF6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (55/72);  new base A (5/3);  total (60/76)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 141 total aligned reads displayed.

CGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTC  >  NC_000913/3044982‑3045278
                                                                                                                                                   |                                                                                                                                                     
 gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                                                                                                    <  2:1147318/150‑1 (MQ=255)
  cTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGc                                                                                                                                                   <  1:951903/150‑1 (MQ=255)
     cGGCTTTATCTATGTGAATAAACATGACGACGGTACTGTCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTTCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTATAATTCTATGCAGTACCGGATGCAATACTTGTTGCATc                                                                                                                                                >  1:1236359/1‑150 (MQ=255)
       gCTTTAGCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCg                                                                                                                                              <  1:505562/150‑1 (MQ=255)
         tttATCTATTTTAATAAACATTAAGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                            <  1:1664196/150‑1 (MQ=255)
         tttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                            <  1:2010487/150‑1 (MQ=255)
           tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                          >  2:1383459/1‑150 (MQ=255)
           tATCTATGTGAAAAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                          <  1:1545712/150‑1 (MQ=255)
            aTCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAg                                                                                                                                         <  2:916389/150‑1 (MQ=255)
            aTCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAg                                                                                                                                         <  1:15060/150‑1 (MQ=255)
              cTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGAc                                                                                                                                       >  1:840697/1‑150 (MQ=255)
              cTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGAc                                                                                                                                       >  2:229448/1‑150 (MQ=255)
               tatgctaaTAAACAAGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTCACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGCTGCATCCGGTCAGACa                                                                                                                                      <  2:390698/144‑1 (MQ=255)
               tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTCCAAAGAGGTGATTGCCAGCAACGTCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                      <  1:1340608/150‑1 (MQ=255)
               tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                      <  1:66690/150‑1 (MQ=255)
                   tgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACtt                                                                                                                                  >  2:896597/1‑150 (MQ=255)
                      aTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTccc                                                                                                                               <  1:1188832/150‑1 (MQ=255)
                       tAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCt                                                                                                                              >  2:1781533/1‑150 (MQ=255)
                         aaCATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGt                                                                                                                            >  1:1511656/1‑150 (MQ=255)
                         aaCATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGt                                                                                                                            >  1:1949977/1‑150 (MQ=255)
                            aTGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGtttt                                                                                                                         <  2:810062/150‑1 (MQ=255)
                             tGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTTGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTa                                                                                                                        >  2:1645632/1‑150 (MQ=255)
                             tGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTa                                                                                                                        <  2:459687/150‑1 (MQ=255)
                             tGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTa                                                                                                                        <  2:1261541/150‑1 (MQ=255)
                              gacgacGGTACTGGCGATATGTCGCGTTCACGTAAGAAGTGCTTTAACTGGTACAAAGTGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAAGACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAt                                                                                                                       <  1:1252910/150‑1 (MQ=255)
                              gacgacGGTACTGGCGATATGTCGCGGTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAACGCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAt                                                                                                                       <  1:777966/150‑1 (MQ=255)
                                cgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAttt                                                                                                                     >  2:398749/1‑150 (MQ=255)
                                   cgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTccc                                                                                                                  <  2:550145/150‑1 (MQ=255)
                                     gTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgc                                                                                                                <  2:1733186/150‑1 (MQ=255)
                                        cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                             <  1:923420/150‑1 (MQ=255)
                                        cTGGCGATATGTCGCGTTCACGTAAAAAGAGCTAAAACTGGTACAAAGAACTGATTGCAAGCAAAGGCGAGAACCTTTAAGTCTATAAAGTACCGGATGCAATACTTGTTGCATACGTTAATACAACTTCCCTGTTATATTTaccgccct                                                                                                             >  1:1082375/1‑149 (MQ=255)
                                          ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                           >  1:1891602/1‑150 (MQ=255)
                                          ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATAGACAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                           >  1:491155/1‑150 (MQ=255)
                                           gCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCa                                                                                                          >  2:1195401/1‑150 (MQ=255)
                                              atatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAAt                                                                                                       <  1:185618/150‑1 (MQ=255)
                                               tatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTTTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                      <  2:1902168/150‑1 (MQ=255)
                                                 tGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGa                                                                                                    <  2:1899859/150‑1 (MQ=255)
                                                  gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGACGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                   >  2:1422070/1‑150 (MQ=255)
                                                  gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                   >  1:1560719/1‑150 (MQ=255)
                                                  gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                   >  1:742847/1‑150 (MQ=255)
                                                  gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGCTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                                   >  1:1520419/1‑150 (MQ=255)
                                                    cgcgTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATaa                                                                                                 <  2:111961/150‑1 (MQ=255)
                                                    cgcgTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAAGCGATaa                                                                                                 <  2:68602/150‑1 (MQ=255)
                                                       gTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAc                                                                                              <  1:272648/150‑1 (MQ=255)
                                                          cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTc                                                                                           >  2:1947115/1‑150 (MQ=255)
                                                              tAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGt                                                                                       >  2:1937321/1‑150 (MQ=255)
                                                               aagaagAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTg                                                                                      >  2:1062432/1‑150 (MQ=255)
                                                                agaagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGa                                                                                     >  1:385088/1‑150 (MQ=255)
                                                                 gaagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGAc                                                                                    <  2:1799026/150‑1 (MQ=255)
                                                                    gagCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTa                                                                                 <  1:314877/150‑1 (MQ=255)
                                                                      gCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAag                                                                               >  2:1102285/1‑150 (MQ=255)
                                                                       cTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAaga                                                                              <  1:1322548/150‑1 (MQ=255)
                                                                          tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                           >  2:411246/1‑150 (MQ=255)
                                                                            aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAATAGTTCCCGTGACGTAAGAGGCtt                                                                         >  1:538010/1‑150 (MQ=255)
                                                                            aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                         <  1:253078/150‑1 (MQ=255)
                                                                              tGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTa                                                                       <  1:598198/150‑1 (MQ=255)
                                                                                gTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATc                                                                     <  1:242419/150‑1 (MQ=255)
                                                                                  aCAAAGAGTTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAc                                                                   <  1:1174489/150‑1 (MQ=255)
                                                                                  aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAc                                                                   <  2:840697/150‑1 (MQ=255)
                                                                                    aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                                 <  2:1484848/150‑1 (MQ=255)
                                                                                    aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                                 <  2:780275/150‑1 (MQ=255)
                                                                                     aaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTta                                                                <  1:1102285/150‑1 (MQ=255)
                                                                                      agagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtag                                                               <  2:1556984/150‑1 (MQ=255)
                                                                                      agagGGGATTGCCAGCAAAGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCTCTTTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtag                                                               <  1:163994/150‑1 (MQ=255)
                                                                                       tagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCTCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagt                                                              <  1:1804105/149‑1 (MQ=255)
                                                                                         ggTCATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGCCAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagtag                                                            >  2:1404866/1‑150 (MQ=255)
                                                                                           tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                          <  2:1986558/150‑1 (MQ=255)
                                                                                           tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAAAACATGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                          >  2:92710/1‑150 (MQ=255)
                                                                                              ttGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATATCCCGCCCGCCAAATCGATAAAACTTCCCGTGAAGTAAGAGGCTTTATCACTTAGTAGCCAgc                                                       >  1:332317/1‑149 (MQ=255)
                                                                                               tGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTTCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGAc                                                      <  2:1959800/150‑1 (MQ=255)
                                                                                                 ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                                    >  2:1008048/1‑150 (MQ=255)
                                                                                                 ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                                    >  2:1757398/1‑150 (MQ=255)
                                                                                                 ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGAAATACTTGTTGCATCCGGACAGACAACTTCCCTGTTTTATTTCCAGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                                    >  2:1775150/1‑150 (MQ=255)
                                                                                                     cAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACATCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGc                                                >  2:1687442/1‑150 (MQ=255)
                                                                                                      aaCGGCGAGAAGCGTTAAGGCGATGAAGTACAGGAGGCAATACTTGTTGCAGCCGGGCAGACAACTTCCCTGTTTTATTTCCCGCCCTCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGcc                                               <  2:1352263/150‑1 (MQ=255)
                                                                                                          gCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCACCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTgcg                                           <  2:417993/150‑1 (MQ=255)
                                                                                                           cGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGGTGCATCCGGTCAGACAACTGCCCTGTTTTATTTCCCGCCCGCCAATTCGATAAAAGTTCCCGTGACGTAAGAGGCTTTATCCCTTAGTAGCCAGACAATTGCCTgcgc                                          >  2:371993/1‑150 (MQ=255)
                                                                                                             agaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGa                                        >  1:619323/1‑150 (MQ=255)
                                                                                                              gaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGTCAACTTCCCTGTTTTATATCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAc                                       <  2:972336/150‑1 (MQ=255)
                                                                                                                   tttAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtctt                                  >  1:425980/1‑150 (MQ=255)
                                                                                                                    ttAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCATACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAATTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttc                                 <  2:1202942/150‑1 (MQ=255)
                                                                                                                    ttAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttc                                 <  1:530375/150‑1 (MQ=255)
                                                                                                                     tAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttct                                <  1:229448/150‑1 (MQ=255)
                                                                                                                         tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCt                            <  1:1781533/150‑1 (MQ=255)
                                                                                                                         tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCt                            <  1:1977862/150‑1 (MQ=255)
                                                                                                                          agATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGGGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTg                           <  2:1272251/149‑1 (MQ=255)
                                                                                                                           gATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGt                          >  1:630276/1‑150 (MQ=255)
                                                                                                                             tGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcc                        <  2:444404/150‑1 (MQ=255)
                                                                                                                              gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcca                       <  1:1645632/150‑1 (MQ=255)
                                                                                                                                 tgaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGAGCGACCGCTTCTGCCTGTccacac                    >  1:124916/3‑148 (MQ=255)
                                                                                                                                  gaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGCGGTAAGCGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCcaccac                   >  2:1401167/2‑150 (MQ=255)
                                                                                                                                  gaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCcaccac                   >  1:791962/2‑150 (MQ=255)
                                                                                                                                      ggATGCAATAATTGTTGCATCCGGTCAGACAACTTCCCTGATTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAAGTAGTAGCCAGACAATGCCCTGCGCGACATCTTCTGACTGTCCACCACGCTg               >  2:1465104/1‑150 (MQ=255)
                                                                                                                                      ggATGCAACAAGTATTGCATCCGGTCAGCCAACTTCCCTGTTTTATTTCACGCCCGCCAAATCGATAAAACTTCCGGTGACGTAAGAGGGTTTATCACTTAGTAGCCAGACACTGGCCTGCGCGACCTCTGCTGCCTGTCCACACCGCTg               >  2:972214/1‑150 (MQ=255)
                                                                                                                                         tGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCCCCACGCTGCAt            >  1:43517/1‑150 (MQ=255)
                                                                                                                                            aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg         <  2:565292/150‑1 (MQ=255)
                                                                                                                                              tACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGa       <  1:1968118/150‑1 (MQ=255)
                                                                                                                                              tACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGa       <  1:1062432/150‑1 (MQ=255)
                                                                                                                                                cTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATg     <  1:896597/150‑1 (MQ=255)
                                                                                                                                                   gttgCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTc  <  2:482806/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTC  >  NC_000913/3044982‑3045278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: