Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,050,715 T→A 6.5% intergenic (‑48/+400) gcvT ← / ← ubiI aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,050,7150TA 6.5% 369.5 / 7.3 129intergenic (‑48/+400)gcvT/ubiIaminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (61/59);  new base A (5/4);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATCGCG  >  NC_000913/3050566‑3050863
                                                                                                                                                     |                                                                                                                                                     
gATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGAAAACTTTTTCATGGTGAAAt                                                                                                                                                       >  1:1252304/1‑150 (MQ=255)
  tttGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGTAATCCACCATGCGTGCGCCGAAAAGCGTGTGTTGTTCGTACAAAGGATGCTGTTGTGCCATCTTGTCCTCATTGAATATGCGTGGCTGACAACTTTTTCATGGTGAAATTa                                                                                                                                                     <  1:956293/150‑1 (MQ=255)
              aaTGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGATAATTTCACCATGaaaa                                                                                                                                         <  1:1732868/150‑2 (MQ=255)
              aaTGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAAc                                                                                                                                         <  1:1388076/150‑1 (MQ=255)
                 gCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAACCCCa                                                                                                                                      >  2:1952880/1‑150 (MQ=255)
                  cAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAg                                                                                                                                     >  1:1156602/1‑150 (MQ=255)
                   aGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCCCGCAACCCAGc                                                                                                                                    >  2:276516/1‑150 (MQ=255)
                   aGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGGGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCGTGGTGATAATTTCACCATGaaaaagttg                                                                                                                                    <  1:1577997/150‑7 (MQ=255)
                       gCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCTGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCg                                                                                                                                <  1:1096919/150‑1 (MQ=255)
                       gCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCCGCATCg                                                                                                                                >  2:1695991/1‑150 (MQ=255)
                          caagccATCCGTGGATATACACCATGCGAGCGCCGCAAATCGGGGGTTGTGCGTACAAAGGAGTCTGTTGTGACATATTGTCCTCATTGAATAAGCGTGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAAACCAGCATCGGAg                                                                                                                             <  2:825472/146‑1 (MQ=255)
                            atcCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGcc                                                                                                                           <  1:212026/150‑1 (MQ=255)
                            atcCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGcc                                                                                                                           <  1:910223/150‑1 (MQ=255)
                              ccAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACTAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCAc                                                                                                                         <  2:701137/150‑1 (MQ=255)
                               cAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACTCAGCATCGGAGCCACt                                                                                                                        >  2:1188582/1‑150 (MQ=255)
                                  ccGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCCCTccc                                                                                                                     >  1:672410/1‑150 (MQ=255)
                                   cGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCCGCATCGGAGCCACTCCCg                                                                                                                    >  2:639050/1‑150 (MQ=255)
                                    gTGGAACTCCACCATGCGAGCGCCGCAAAGCGGGTGTTGGTCGTACAAAGGAGTCTGTTGGGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCgg                                                                                                                   <  2:1231228/150‑1 (MQ=255)
                                    gTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCgg                                                                                                                   >  2:906334/1‑150 (MQ=255)
                                     tGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGCAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATAATCACCACGAAACCCAGCATCGGAGCCACACCCGGt                                                                                                                  >  1:1551991/1‑150 (MQ=255)
                                      ggAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTc                                                                                                                 <  1:1931510/150‑1 (MQ=255)
                                       gAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTcc                                                                                                                <  1:1388190/150‑1 (MQ=255)
                                        aaaTCCACCATGCGAGCGCCGCAAAGCGGGGGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTccc                                                                                                               <  2:977602/150‑1 (MQ=255)
                                          aTCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCa                                                                                                             <  1:1642005/150‑1 (MQ=255)
                                            ccaccaTGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGTTGCTGACATCTTTTTCATGGTGAAATTATCACCACGAAACGCAGCATCGGAGCCACTCCCGGTCCCCAAc                                                                                                           <  2:1162172/150‑1 (MQ=255)
                                            ccaccaTGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAAc                                                                                                           <  2:1185103/150‑1 (MQ=255)
                                             caccaTGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                                                          >  1:747406/1‑150 (MQ=255)
                                                 aTGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTAGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAAt                                                                                                      <  2:1102954/150‑1 (MQ=255)
                                                  tGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATc                                                                                                     >  2:1153374/1‑150 (MQ=255)
                                                   gCGACCGCCGCAAAGCGGGTGTTGTTCGTACAAAGGAGGCTGTTGTGCCATCTTGGCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCg                                                                                                    <  1:1142692/150‑1 (MQ=255)
                                                    cGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGt                                                                                                   >  2:1631438/1‑150 (MQ=255)
                                                      aGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTtc                                                                                                 <  1:1230653/150‑1 (MQ=255)
                                                        ctccgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTTTTGTGCCATCTTGTCCTCATTGAATAAGCGGGTCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTtctc                                                                                               <  1:1239168/148‑1 (MQ=255)
                                                         gccgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTctct                                                                                              >  2:103251/1‑150 (MQ=255)
                                                          ccgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCtt                                                                                             <  1:1804346/150‑1 (MQ=255)
                                                           cgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCACCGGAATCGTTCTCttt                                                                                            >  2:157066/1‑150 (MQ=255)
                                                           cgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCACCGCAATCGTTCTCttt                                                                                            >  2:878446/1‑150 (MQ=255)
                                                            gcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGAGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCtttt                                                                                           >  1:597038/1‑150 (MQ=255)
                                                             ctaattgtgtgtTTTTCGTACAATGGAGTCTGTTGTGCCATGTTTTCCTCATTGAATAAGAGGGGCTGTCTACTTTTTCATGGTGAAATTATCACCAGGAATCCCAGCATCGGATCCACTCCAGGTCCCCAACGCAATCGTTCTCTTTTg                                                                                          <  1:2009130/144‑1 (MQ=255)
                                                                aGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCt                                                                                       <  2:1274221/150‑1 (MQ=255)
                                                                   gtgtgtTGTTCGTACTTAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAATTTATCACCACGAAACCCAGGATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGaa                                                                                    <  2:1619499/150‑1 (MQ=255)
                                                                    tgtgtTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGTGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGTGCCTCTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAAc                                                                                   <  1:1040964/150‑1 (MQ=255)
                                                                    tgtgtTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAAc                                                                                   <  1:85193/150‑1 (MQ=255)
                                                                    tgtgtTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAAc                                                                                   <  1:1972165/150‑1 (MQ=255)
                                                                    tgtgtTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAAc                                                                                   <  2:230400/150‑1 (MQ=255)
                                                                     gtgtTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACt                                                                                  <  2:322443/150‑1 (MQ=255)
                                                                       gttgttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTa                                                                                >  2:1586562/1‑150 (MQ=255)
                                                                          gttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCCCCACGAAACACAGCATCGGCGCCACTCACGGTCCCCAACTCACTCGTTCTCTTTTGCATGAACTTcccc                                                                             >  1:1223161/1‑149 (MQ=255)
                                                                           ttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTaccac                                                                            <  2:758613/150‑1 (MQ=255)
                                                                           ttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTaccac                                                                            >  1:1149890/1‑150 (MQ=255)
                                                                            tCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCTTCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTATGCCTGAACTTaccacc                                                                           >  2:488916/1‑150 (MQ=255)
                                                                             cGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCg                                                                          >  1:1849901/1‑150 (MQ=255)
                                                                              gTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGa                                                                         >  2:794543/1‑150 (MQ=255)
                                                                                 cAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAAc                                                                      >  1:862129/1‑150 (MQ=255)
                                                                                 cAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAAc                                                                      >  1:667855/1‑150 (MQ=255)
                                                                                   aaGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAg                                                                    >  1:1953454/1‑150 (MQ=255)
                                                                                     ggAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGAc                                                                  <  1:1313270/150‑1 (MQ=255)
                                                                                        gTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGt                                                               >  1:1670880/1‑150 (MQ=255)
                                                                                              tgtgCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCa                                                         <  1:1631438/150‑1 (MQ=255)
                                                                                                   cATCTTGTCCTCATTGAATAAGCTGGGCTGACAACTTTATCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGGCCCCAACGCACTCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAgg                                                    <  2:154545/150‑1 (MQ=255)
                                                                                                     tCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTa                                                  >  2:1287598/1‑150 (MQ=255)
                                                                                                          tCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAAtt                                             <  2:1814598/150‑1 (MQ=255)
                                                                                                          tCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAAtt                                             >  2:570099/1‑150 (MQ=255)
                                                                                                            cTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGa                                           >  2:1484788/1‑150 (MQ=255)
                                                                                                            cTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTATCTTTTTCCTGAACTTACCACCGAAACAGACTGTTAACCAAAATGTAAAATTGa                                           >  2:1848597/1‑150 (MQ=255)
                                                                                                            cTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATAACCACGAAACCCAGCATCGGAGCCACTCACGGTCCCCAACGCAATCGTTCTATTTTGCCTGAACTTACCACCGAAACAGACAGTTAACCATAAGGTAAAATTGa                                           >  1:1861408/1‑150 (MQ=255)
                                                                                                              cATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatc                                         <  2:1473679/150‑1 (MQ=255)
                                                                                                               aTTGAATAAGCGGGGCTGACAACTTTTTCATTGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatca                                        <  2:493204/150‑1 (MQ=255)
                                                                                                               aTTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATAACCACGAAACCCAGCATCGGAGCCACTCACGGTCCCCAACGCAAACGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTATACCATAAGGTAAAATTGatca                                        >  1:1226868/1‑150 (MQ=255)
                                                                                                                  gAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACCGACTGTTAACCATAAGGTAAAATTGAtcatca                                    <  2:1037509/150‑1 (MQ=255)
                                                                                                                  gAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGAtcatca                                    >  1:429691/1‑150 (MQ=255)
                                                                                                                   aaTAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATcac                                   <  2:1474383/150‑1 (MQ=255)
                                                                                                                   aaTAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATcac                                   <  2:1131240/150‑1 (MQ=255)
                                                                                                                    aTAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATcaca                                  >  1:957245/1‑150 (MQ=255)
                                                                                                                     tAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGGTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACAt                                 >  1:964726/1‑150 (MQ=255)
                                                                                                                       aGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTa                               >  2:1544481/1‑150 (MQ=255)
                                                                                                                        gCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAg                              <  1:909198/150‑1 (MQ=255)
                                                                                                                         cGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGc                             >  1:458728/1‑150 (MQ=255)
                                                                                                                          ggggCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCt                            <  2:1156602/150‑1 (MQ=255)
                                                                                                                          ggggCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCt                            >  1:1721847/1‑150 (MQ=255)
                                                                                                                          ggggCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGACACTCCCGGTCCCCAACGCAATCGTTCTCATTTGCCTGAACTAACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATAACATTAGCt                            >  2:1427396/1‑150 (MQ=255)
                                                                                                                               tGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATgg                       <  2:1136895/150‑1 (MQ=255)
                                                                                                                               tGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATgg                       <  2:1727493/150‑1 (MQ=255)
                                                                                                                               tGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGACACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATgg                       <  1:386999/150‑1 (MQ=255)
                                                                                                                                 tgctgggtttCGTGGTGATAATTTCACCATGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGtt                     >  2:491025/8‑150 (MQ=255)
                                                                                                                                 aCAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTGATGGtt                     >  2:1380582/1‑150 (MQ=255)
                                                                                                                                    tgggtttCGTGGTGATAATTTCACCATGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaa                  >  1:588921/5‑150 (MQ=255)
                                                                                                                                    aCTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaa                  >  2:1912529/1‑150 (MQ=255)
                                                                                                                                     cTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCTTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGTTTaaaa                 >  2:280340/1‑150 (MQ=255)
                                                                                                                                     cTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaaa                 >  2:1764092/1‑150 (MQ=255)
                                                                                                                                      tttttCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGCTAATTTGATGATCAATTTTACATTATGGTTaacag                <  1:1449273/150‑4 (MQ=255)
                                                                                                                                      tttttCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTATCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaaaa                >  2:1008095/1‑150 (MQ=255)
                                                                                                                                       gtttCGTGGTGATAATTTCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACGACCGAAACAGACGGTTAACAATAAGGTAAAATTGATCATCACTTTAGCTTATGGTTaaaaaa               >  1:1024830/2‑150 (MQ=255)
                                                                                                                                         ttCATTGTGAAAATATCAAAACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTTAACTTACCACCGAAACCGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGGTAAAAAATg             <  2:1718548/150‑1 (MQ=255)
                                                                                                                                          tCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTTCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGc            <  2:1223161/150‑1 (MQ=255)
                                                                                                                                             tGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACTCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCaaa         <  1:1433592/150‑1 (MQ=255)
                                                                                                                                              ggTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCaaaa        >  1:554294/1‑150 (MQ=255)
                                                                                                                                              ggTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCaaaa        >  2:1912831/1‑150 (MQ=255)
                                                                                                                                                 gAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATc     <  1:1056242/150‑1 (MQ=255)
                                                                                                                                                    aTTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATcgcg  <  1:1695991/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATCGCG  >  NC_000913/3050566‑3050863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: