Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,066,310 A→T 5.2% H288Q (CAT→CAA ygfI ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,066,3100AT 5.2% 515.8 / 10.1 166H288Q (CAT→CAAygfIputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (79/78);  new base T (3/6);  total (82/84)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 169 total aligned reads displayed.

TATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATG  >  NC_000913/3066161‑3066459
                                                                                                                                                      |                                                                                                                                                     
tATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCTCGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAata                                                                                                                                                       <  2:507699/150‑1 (MQ=255)
tATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAata                                                                                                                                                       <  2:1510297/150‑1 (MQ=255)
   cGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGc                                                                                                                                                    >  2:1239940/1‑150 (MQ=255)
   cGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGc                                                                                                                                                    <  1:1278807/150‑1 (MQ=255)
    gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCt                                                                                                                                                   >  1:1862523/1‑150 (MQ=255)
    gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCt                                                                                                                                                   >  1:1882861/1‑150 (MQ=255)
    gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCt                                                                                                                                                   <  2:212110/150‑1 (MQ=255)
      gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGa                                                                                                                                                 <  2:589052/150‑1 (MQ=255)
       aTTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGaa                                                                                                                                                <  2:1489372/150‑1 (MQ=255)
        ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAg                                                                                                                                               <  1:1143230/150‑1 (MQ=255)
        ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAg                                                                                                                                               <  1:221212/150‑1 (MQ=255)
         tATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGCTCTTAGCCACGTCGTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTACTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGc                                                                                                                                              >  2:1136613/1‑150 (MQ=255)
         tATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGc                                                                                                                                              <  1:111006/150‑1 (MQ=255)
          aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCa                                                                                                                                             >  1:1429327/1‑150 (MQ=255)
            cttcATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCCCCCCGATCTTAGCCACGTCTTCCACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCCGCATATTGCTGAAgatcg                                                                                                                                           <  2:1833329/147‑5 (MQ=255)
             tGCATCGCTATCTTGAAAATGCGCCTTGCGGACATATTCACCACGATCTTAGCCACGTCTTGGACTTACACCGTAATTTAATTGCAACCGGCGCGATGCTTGGTTAAGGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAgatcg                                                                                                                                           <  1:614900/150‑5 (MQ=255)
             tGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCATATTGCTGAAGATCGt                                                                                                                                         <  1:840708/150‑1 (MQ=255)
               cATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTg                                                                                                                                        >  2:1723406/1‑150 (MQ=255)
                   gCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCgg                                                                                                                                    >  2:301660/1‑150 (MQ=255)
                   gCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCgg                                                                                                                                    >  2:966939/1‑150 (MQ=255)
                   gCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACgg                                                                                                                                    <  1:1898081/150‑1 (MQ=38)
                   gCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACgg                                                                                                                                    <  2:168221/150‑1 (MQ=38)
                     tATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGta                                                                                                                                  <  1:13713/150‑1 (MQ=255)
                        cTGGAAAATGCGCCTTGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTTAAGCATCGTGGCGGTATaa                                                                                                                               <  2:455721/150‑1 (MQ=255)
                         tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                                                              >  1:1053256/1‑150 (MQ=255)
                         tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                                                              <  2:625409/150‑1 (MQ=255)
                          ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACg                                                                                                                             >  2:676564/1‑150 (MQ=255)
                          ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACg                                                                                                                             <  1:1518158/150‑1 (MQ=255)
                              aaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACgatga                                                                                                                         >  2:1937119/1‑150 (MQ=255)
                               aTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACgattat                                                                                                                        <  1:596464/150‑1 (MQ=255)
                                   gcCTGGCGGACATATTCACCACGATCTTCGCCTCGTCTTCGACTTACACCGTAATTTAATTGCATCCGGCTCGATGCGGGGTTAATGACTCGTCGCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCt                                                                                                                    <  2:728527/150‑1 (MQ=255)
                                   gcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCGGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCt                                                                                                                    <  1:1916702/150‑1 (MQ=255)
                                        gCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTg                                                                                                               <  2:1262492/150‑1 (MQ=255)
                                         cGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGa                                                                                                              <  1:2005027/150‑1 (MQ=255)
                                           gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                                                                            <  1:837067/150‑1 (MQ=255)
                                           gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                                                                            <  1:1580526/150‑1 (MQ=255)
                                              atatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAAAGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                                                         >  1:1316319/1‑150 (MQ=255)
                                               tatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTTATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATTATTTCTGATTGAATAAAg                                                                                                        >  2:365328/1‑150 (MQ=255)
                                               tatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                        >  2:654543/1‑150 (MQ=255)
                                                atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                                                                       >  2:1440412/1‑150 (MQ=255)
                                                atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                                                                       >  2:458184/1‑150 (MQ=255)
                                                atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                                                                       >  1:1633207/1‑150 (MQ=255)
                                                   caccacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGatat                                                                                                    <  2:2009337/150‑1 (MQ=255)
                                                       acGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCt                                                                                                >  1:1624160/1‑150 (MQ=255)
                                                        cGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTg                                                                                               >  2:1599777/1‑150 (MQ=255)
                                                            cTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGTTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCata                                                                                           <  1:143866/150‑1 (MQ=255)
                                                            cTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCTGTATAACTATGATTTCTGATTGAATAAATATATTCCTGCata                                                                                           >  2:1327383/1‑150 (MQ=255)
                                                                 ccACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACAt                                                                                      <  1:2014579/150‑1 (MQ=255)
                                                                    cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCgg                                                                                   <  2:1360602/150‑1 (MQ=255)
                                                                     gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGCAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGc                                                                                  >  1:1248198/1‑150 (MQ=255)
                                                                     gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGc                                                                                  >  2:693654/1‑150 (MQ=255)
                                                                      tCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGcc                                                                                 <  1:654543/150‑1 (MQ=255)
                                                                       cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGTTATAACGATGATTTCTGATTGAATAAAGATATTCATGCATATACATCGGCCt                                                                                >  1:250107/1‑150 (MQ=255)
                                                                       cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAAAAAGATATTCCTGCATATACATCGGCCt                                                                                >  1:989391/1‑150 (MQ=255)
                                                                           gACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGc                                                                            <  1:722905/150‑1 (MQ=255)
                                                                             cTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg                                                                          <  2:1280587/150‑1 (MQ=255)
                                                                               tACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTCCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                                                                        <  2:1445605/150‑1 (MQ=255)
                                                                                       aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                                                                <  1:1660042/150‑1 (MQ=255)
                                                                                          ttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAatat                                                             >  2:825323/1‑150 (MQ=255)
                                                                                           taattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCGGCATATACATCGGCCTGTGCGGTGCATCAAAATAtt                                                            >  1:1784758/1‑150 (MQ=255)
                                                                                             attGCACCCGGCTAGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGGGGCGGTATAACGATGATTTCTGATTGAATCTAGATATTCCTGCATCTACATCGGCCTGTGCGGTGCATCAAAATATTTc                                                          <  1:1807530/150‑1 (MQ=255)
                                                                                                gCAACCTGCACTATTCTCGGAAAATAACTCGTACCGTCACGATCTAACGCAATTTGCACAAAAATCTTGGCGTAATTACGATGAATAATGATTGAAAAAAGATATTCCTGCATATACATCGGCCTGTGCGTTGAATCAAAATATTTCAGc                                                       >  2:1886842/1‑150 (MQ=255)
                                                                                                 cAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCg                                                      >  1:797100/1‑150 (MQ=255)
                                                                                                 cAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCg                                                      >  1:1931251/1‑150 (MQ=255)
                                                                                                              gCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAc                                         >  1:1975079/1‑150 (MQ=255)
                                                                                                               cTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAcc                                        <  2:492097/150‑1 (MQ=255)
                                                                                                               cTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAcc                                        <  1:1239940/150‑1 (MQ=255)
                                                                                                                  ggTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGt                                     <  1:70799/150‑1 (MQ=255)
                                                                                                                   gTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATATAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTc                                    >  1:713536/1‑150 (MQ=255)
                                                                                                                      aaTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGcc                                 <  2:923709/150‑1 (MQ=255)
                                                                                                                       aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCa                                >  2:1369543/1‑150 (MQ=255)
                                                                                                                       aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCa                                <  2:1448386/150‑1 (MQ=255)
                                                                                                                       aTGACTCGTCCAGTCACGATCTTCAGCAATATGCTAAAGCATCGTGGCGGTATAACGATGATTTCTGATTGTATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCa                                <  1:1216890/150‑1 (MQ=255)
                                                                                                                         gACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAgg                              <  1:1222411/150‑1 (MQ=255)
                                                                                                                          aCTAGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGc                             <  1:773580/150‑1 (MQ=255)
                                                                                                                           cTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCa                            >  2:1711916/1‑150 (MQ=255)
                                                                                                                            tCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCaa                           >  1:551619/1‑150 (MQ=255)
                                                                                                                                cccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAcc                                        >  2:918888/1‑133 (MQ=255)
                                                                                                                                cccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAcc                                        <  1:918888/133‑1 (MQ=255)
                                                                                                                                  cgccACGATGCTTCAGCATATTGCTGAAGATCGTGAAGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACAAGTCGCCAGGCAAAATGCg                     >  2:1649816/4‑150 (MQ=38)
                                                                                                                                   gTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCgg                    >  2:913413/1‑150 (MQ=255)
                                                                                                                                    tCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCggg                   <  1:759200/150‑1 (MQ=255)
                                                                                                                                     cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGa                  <  1:301660/150‑1 (MQ=255)
                                                                                                                                     cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGa                  <  1:774253/150‑1 (MQ=255)
                                                                                                                                     cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGa                  >  2:1081557/1‑150 (MQ=255)
                                                                                                                                     cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGa                  >  2:1175707/1‑150 (MQ=255)
                                                                                                                                       cGATCTTCCGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGGATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCCGTCGCCAGGCAAAATGCGGGATc                >  1:962302/1‑150 (MQ=255)
                                                                                                                                         aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCAc              <  1:1599777/150‑1 (MQ=255)
                                                                                                                                           gcTTCAGCATATTGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAATATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc             >  1:1681275/2‑150 (MQ=255)
                                                                                                                                            cTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGATATGCAAAATGGGGGATCACCg            <  2:260038/150‑1 (MQ=255)
                                                                                                                                             ttCAGCAAAATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCGTGCATATACATCGGCCTGTGAGGTGCATCAAAATATATCAGCGTTGCCGGATTCACAAGTCGCCAGGAAAAATGCGGGATCACCGt           >  1:1353846/1‑150 (MQ=255)
                                                                                                                                              tctgCAAAATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTAGCCGGATTCACAAGTCGCCAGGCAAAATGCGGGATCACCATc          >  2:720743/4‑150 (MQ=255)
                                                                                                                                              tCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTc          <  1:966939/150‑1 (MQ=255)
                                                                                                                                              tCAGCAATATGCTGAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCa         <  1:44494/150‑1 (MQ=255)
                                                                                                                                                aGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAg        <  1:1723406/150‑1 (MQ=255)
                                                                                                                                                aGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAg        <  2:1862523/150‑1 (MQ=255)
                                                                                                                                                 gCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGa       >  2:593646/1‑150 (MQ=255)
                                                                                                                                                  cAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaa      <  1:458184/150‑1 (MQ=255)
                                                                                                                                                  cAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaa      <  1:1440412/150‑1 (MQ=255)
                                                                                                                                                      atGCTGAAGAATCGTGGCGGTATAACGATGATTTCTGATTGACTAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATg  <  2:292587/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATG  >  NC_000913/3066161‑3066459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: