Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 282,518 G→A 6.9% G81S (GGC→AGC)  yagE → 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913282,5180GA 6.9% 499.3 / 15.9 161G81S (GGC→AGC) yagE2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base G (70/78);  new base A (4/7);  total (75/86)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 163 total aligned reads displayed.

CGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGAC  >  NC_000913/282369‑282664
                                                                                                                                                     |                                                                                                                                                  
cgcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCg                                                                                                                                                    <  1:1467313/150‑1 (MQ=255)
 gcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTATGCTATCGATCATGTCGCTCGTCGCGTGCCGGTGCAGATCgg                                                                                                                                                   >  2:181686/1‑150 (MQ=255)
    gATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAc                                                                                                                                                >  1:422747/1‑150 (MQ=255)
    gATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAc                                                                                                                                                >  2:788050/1‑150 (MQ=255)
     aTCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAcc                                                                                                                                               <  2:1599676/150‑1 (MQ=255)
       cgacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcgg                                                                                                                                             >  2:323827/1‑150 (MQ=255)
        gacgaTCTGATTAAAGCAGGCGTAGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc                                                                                                                                            <  1:449765/150‑1 (MQ=255)
        gacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc                                                                                                                                            <  2:825998/150‑1 (MQ=255)
        gacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTCGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc                                                                                                                                            <  1:86166/150‑1 (MQ=255)
         acgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggca                                                                                                                                           <  2:1530707/150‑2 (MQ=255)
          cgaTCTGATCAAAGCAGGCGGTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggcac                                                                                                                                          <  1:1219888/150‑3 (MQ=255)
            aTCTGCTCAACGCAGGCGGTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa                                                                                                                                        <  1:1404520/150‑1 (MQ=255)
            aTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa                                                                                                                                        >  1:262279/1‑150 (MQ=255)
            aTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa                                                                                                                                        >  1:1508929/1‑150 (MQ=255)
                gATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCaa                                                                                                                                    >  1:869970/1‑150 (MQ=255)
                  tCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGAAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGAGCAAGTCGATCGTCGCGTTCAGGTGCTCATCTGCACCGGCTGCACCAACg                                                                                                                                  >  1:1191219/1‑150 (MQ=255)
                   cAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCTAGTTCTCCCAGCTCGGCGCCGAAGAGCGAAAAGCCATTGCCCGCTTTGCAATCGATAATGTCGATCGTAGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGc                                                                                                                                 >  1:1285545/1‑150 (MQ=255)
                       gCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCgg                                                                                                                             <  1:482401/150‑1 (MQ=255)
                       gCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCAAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCgg                                                                                                                             <  1:1039073/150‑1 (MQ=255)
                        cAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCggg                                                                                                                            <  2:2001130/150‑1 (MQ=255)
                           gCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGaaa                                                                                                                         >  1:267938/1‑150 (MQ=255)
                           gCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGaaa                                                                                                                         >  1:60837/1‑150 (MQ=255)
                            cGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc                                                                                                                        <  1:1931579/150‑1 (MQ=255)
                             gTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAcc                                                                                                                       <  1:677404/150‑1 (MQ=255)
                              ttGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCGTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCa                                                                                                                      <  1:1418697/150‑1 (MQ=255)
                                gACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATc                                                                                                                    <  1:317466/150‑1 (MQ=255)
                                 aCGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCg                                                                                                                   <  2:630301/150‑1 (MQ=255)
                                   ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAATCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGTCACCGGCGGCACCAACGCCCGGGAAACCATCGaa                                                                                                                 <  1:1060115/150‑1 (MQ=255)
                                    gCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGCTCATGTCGCTCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCCCCAACGCCCGGGCACCCATCGCAc                                                                                                                >  1:354970/1‑150 (MQ=255)
                                    gCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAAc                                                                                                                <  2:1920616/150‑1 (MQ=255)
                                            ttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcagccag                                                                                                        >  2:798942/1‑150 (MQ=255)
                                               cTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGGAACCATCGAACTCAGCCAGCAc                                                                                                     >  2:622769/1‑150 (MQ=255)
                                                   gCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACgcgc                                                                                                 <  1:812428/150‑1 (MQ=255)
                                                         gTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCATCGCCCGGGACACCATCGAACTCAGCCAGCACGCGCCGCAgg                                                                                           >  1:953674/1‑150 (MQ=255)
                                                           ggCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcg                                                                                         >  2:397630/1‑150 (MQ=255)
                                                           ggCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcg                                                                                         >  1:869725/1‑150 (MQ=255)
                                                            gCGAGGTCTCCCAGCTCGGCGCCGAAGACCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGAGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcgg                                                                                        <  2:768480/150‑1 (MQ=255)
                                                             cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCCCCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCCGCACGCGCAGCCggcggg                                                                                       >  1:1847396/1‑150 (MQ=255)
                                                             cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACTGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcggg                                                                                       <  1:1163630/150‑1 (MQ=255)
                                                             cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcggg                                                                                       >  2:1299567/1‑150 (MQ=255)
                                                               aGTTCTCCCAGCCCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAggcgggcg                                                                                     <  2:1020805/150‑1 (MQ=255)
                                                                  tctcCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCgg                                                                                  <  1:833068/150‑1 (MQ=255)
                                                                     cccAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCACCGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACg                                                                               >  1:1980270/1‑150 (MQ=255)
                                                                      ccAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACgg                                                                              <  2:1818994/150‑1 (MQ=255)
                                                                         gCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGCTCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCAt                                                                           >  1:1385408/1‑150 (MQ=255)
                                                                           tCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCCGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCg                                                                         >  2:471281/1‑150 (MQ=255)
                                                                           tCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCg                                                                         >  1:888304/1‑150 (MQ=255)
                                                                           tCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCg                                                                         <  2:262279/150‑1 (MQ=255)
                                                                            cGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgt                                                                        >  2:423001/1‑150 (MQ=255)
                                                                            cGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgt                                                                        >  2:1149058/1‑150 (MQ=255)
                                                                             ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg                                                                       >  1:763067/1‑150 (MQ=255)
                                                                             ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg                                                                       >  1:1478215/1‑150 (MQ=255)
                                                                             ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg                                                                       >  2:1571653/1‑150 (MQ=255)
                                                                               cgcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtggt                                                                     <  2:915388/150‑1 (MQ=255)
                                                                               cgcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtggt                                                                     >  1:458046/1‑150 (MQ=255)
                                                                                 ccGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtggtgc                                                                   >  1:361983/1‑149 (MQ=255)
                                                                                     agagCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCaa                                                               >  2:1492216/1‑150 (MQ=255)
                                                                                       agCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAAcc                                                             >  2:1403825/1‑150 (MQ=255)
                                                                                        gCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAAcct                                                            <  2:217670/150‑2 (MQ=255)
                                                                                         cGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAAcccc                                                           >  1:243847/1‑150 (MQ=255)
                                                                                          gTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGACACCATCGAACTCAGCCAGCCCGCGCAGCAGGCGGGCGCGGCCGGCATCGTGGTGATCAACCCct                                                          >  1:1006231/1‑150 (MQ=255)
                                                                                            aaaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCctac                                                        <  1:1707331/150‑1 (MQ=255)
                                                                                            aaaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCctac                                                        >  2:1742300/1‑150 (MQ=255)
                                                                                             aaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCctact                                                       <  2:1162007/150‑1 (MQ=255)
                                                                                              aGCCATTGCCCGCGTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCCCCCACGCCCGGGAACCCATCGAACGCCGCCAGCACGCGCAGCAGGAGGGCGCGGACGGCATCGTGGTGCTCAACCCctacta                                                      >  2:157946/1‑150 (MQ=255)
                                                                                                ccATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTactact                                                    <  2:359857/150‑1 (MQ=255)
                                                                                                   ttGCCCGCTTTGCTATCGAGCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACGCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGTATCGTGGTGATCAACCCCTACTACTGGa                                                 <  1:1017347/150‑1 (MQ=255)
                                                                                                    tGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACTGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa                                                <  2:479347/150‑1 (MQ=255)
                                                                                                    tGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa                                                <  1:32750/150‑1 (MQ=255)
                                                                                                    tGCCCGCTTTGCTATCGATCATGTCGAGCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa                                                <  1:1555678/150‑1 (MQ=255)
                                                                                                      cgcgcgTTGCTATCGATCATGTCGTTCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTTGTGATCAACCCCTACTACTGGAAAg                                              <  2:134950/148‑1 (MQ=255)
                                                                                                      cccGCTTTGCTATCGATCATGTCGATCGCCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCACGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAg                                              >  2:1828275/1‑150 (MQ=255)
                                                                                                       ccGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAgt                                             >  2:1413398/1‑150 (MQ=255)
                                                                                                       ccGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAgt                                             >  2:1836448/1‑150 (MQ=255)
                                                                                                         gCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAgtgt                                           <  2:754584/150‑1 (MQ=255)
                                                                                                          cTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTc                                          >  1:474917/1‑150 (MQ=255)
                                                                                                           tttGCTATCGTTCATGTCGATCGTCGCGTGCCTGTGCTGATCTGCACCGGCGGCACCAACGCCAGGGAAACCATCGCACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCg                                         <  1:1229601/150‑1 (MQ=255)
                                                                                                             tGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGCCGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGa                                       >  1:606010/1‑150 (MQ=255)
                                                                                                                tATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGc                                    <  2:898936/150‑1 (MQ=255)
                                                                                                                 aTCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCg                                   >  1:1649485/1‑150 (MQ=255)
                                                                                                                     aTCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAAcc                               <  1:1389440/150‑1 (MQ=255)
                                                                                                                     aTCATGTCGATCGTCGCGTGACGGTGCTGATCGGCTCCTGCGTCACAAACGCCCGGGTAACGATCGAACTGAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAAcc                               <  2:1285545/150‑1 (MQ=255)
                                                                                                                      tCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCt                              <  1:112366/150‑1 (MQ=255)
                                                                                                                      tCATGTCGATCGTCGCGTGCCGGTGCGGATCGGCACAGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCt                              <  2:392171/150‑1 (MQ=255)
                                                                                                                            ggATCGTCGCGTGCCGGTGCGGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCg                        <  1:1751676/149‑1 (MQ=255)
                                                                                                                                  tcgCGTGCCGGTGCTGAGCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTAttt                  <  2:946689/150‑1 (MQ=255)
                                                                                                                                    gcgTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCg                >  1:342650/1‑150 (MQ=255)
                                                                                                                                    gcgTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCg                <  2:267938/150‑1 (MQ=255)
                                                                                                                                      gTGCCGGTGCGGAGCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAg              <  1:920401/150‑1 (MQ=255)
                                                                                                                                       cgCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGc             >  1:1665901/2‑150 (MQ=255)
                                                                                                                                        gCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCa            <  1:397630/150‑1 (MQ=255)
                                                                                                                                        gCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCGACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCgcgcg            >  2:1976798/1‑149 (MQ=255)
                                                                                                                                        gCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGCGCa            >  2:281916/1‑150 (MQ=255)
                                                                                                                                          gggtggtGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAgg          <  2:1694314/149‑1 (MQ=255)
                                                                                                                                           ggTGCTGATCGGCACCGGCGGCACCAACTCCCGGGAAACCATCGAACTCAGACAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCACTATTTCGATCAGGt         <  1:77918/150‑1 (MQ=255)
                                                                                                                                              gCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGCTCCGCTATTTCGAGCAGGTGGc      >  2:180207/1‑150 (MQ=255)
                                                                                                                                               cTGATCGGCACCTGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTAATGGATAGTGTCGGAAGAGAACATGATCCGCTATTACGAGCAGGTTGcc     >  2:1761403/1‑150 (MQ=255)
                                                                                                                                               cTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGCACCTGATCCGCTATTTCGAGCAGGTGGcc     >  1:2003721/1‑150 (MQ=255)
                                                                                                                                               cTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGcc     >  2:586107/1‑150 (MQ=255)
                                                                                                                                                 gATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGa   >  2:771653/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGAC  >  NC_000913/282369‑282664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: