Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,101,656 T→A 5.0% intergenic (‑33/+151) yggN ← / ← yggL DUF2884 family putative periplasmic protein/DUF469 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,6560TA 5.0% 434.8 / 3.6 145intergenic (‑33/+151)yggN/yggLDUF2884 family putative periplasmic protein/DUF469 family protein
Reads supporting (aligned to +/- strand):  ref base T (71/64);  new base A (4/5);  total (75/70)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

CGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGCTT  >  NC_000913/3101509‑3101804
                                                                                                                                                   |                                                                                                                                                    
cGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTg                                                                                                                                                    >  1:464145/1‑150 (MQ=255)
      gCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcc                                                                                                                                              <  1:1419721/150‑1 (MQ=255)
       cGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcct                                                                                                                                             <  1:1766433/150‑2 (MQ=255)
        gggCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctg                                                                                                                                            <  1:137528/150‑3 (MQ=255)
          gCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAg                                                                                                                                          >  2:633271/1‑150 (MQ=255)
           cTGACAATCACATCGTCACGCGGCGTGACGCTGCTCTGGTAGTCGGTGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                                                                                                         <  1:1370376/150‑1 (MQ=255)
           cTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGGAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                                                                                                         >  2:1060846/1‑150 (MQ=255)
           cTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                                                                                                         >  1:720684/1‑150 (MQ=255)
            tGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                                                                                                        >  2:129714/1‑150 (MQ=255)
            tGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                                                                                                        >  2:1429837/1‑150 (MQ=255)
                  tCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                                                                                                  >  1:450120/1‑150 (MQ=255)
                   cacaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCCCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAGTTGCTGGCCCCCGATTGagatc                                                                                                                                 >  1:1189273/1‑146 (MQ=255)
                     cagcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                                                                                                               >  2:1838437/4‑150 (MQ=255)
                      aTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa                                                                                                                              <  1:151654/150‑1 (MQ=255)
                        cGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                                                                                                            >  2:472950/1‑150 (MQ=255)
                           cACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg                                                                                                                         >  1:1761837/1‑150 (MQ=255)
                             cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                                                                                                       <  1:1434354/150‑1 (MQ=255)
                             cgcgGCGTGACGCTGCACTGGGAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCAAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                                                                                                       <  1:280500/150‑1 (MQ=255)
                                  cgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                                                                                                  >  2:1829526/1‑150 (MQ=255)
                                   gggACGCTGCACGGGTAGGCGGCGTGAGCGGTCATTGCCGGCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAACTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                                                                                                 <  2:1255075/148‑1 (MQ=255)
                                   gTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                                                                                                 <  1:1595755/150‑1 (MQ=255)
                                        gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                                                                                                            <  1:1982659/150‑1 (MQ=255)
                                        gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                                                                                                            <  1:1330536/150‑1 (MQ=255)
                                        gCTGCACTGGTAGTCGGCGTGAGCGGGCATTGCCGGCACTGAAAGTGCAGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACACAGTACGCGTTTTCCATGcc                                                                                                            <  2:307235/150‑1 (MQ=255)
                                         cTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                                                                                                           <  1:587475/150‑1 (MQ=255)
                                          tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                                                                                                          >  1:904276/1‑150 (MQ=255)
                                          tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                                                                                                          <  1:1630619/150‑1 (MQ=255)
                                           gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                                                                                                         >  2:1555264/1‑150 (MQ=255)
                                               tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg                                                                                                     <  2:1746774/150‑1 (MQ=255)
                                                ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg                                                                                                    <  1:751443/150‑1 (MQ=255)
                                                ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg                                                                                                    >  1:68918/1‑150 (MQ=255)
                                                    gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCCGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTa                                                                                                >  1:1247222/1‑150 (MQ=255)
                                                     tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaa                                                                                               <  1:1474293/150‑1 (MQ=255)
                                                           tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt                                                                                         <  1:528657/150‑1 (MQ=255)
                                                           tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCAAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCAGGATTCGGCGTAAACACCt                                                                                         >  2:1138439/1‑150 (MQ=255)
                                                           ggAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATATGCGGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt                                                                                         <  2:45638/149‑1 (MQ=255)
                                                               cGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAATGATAACAAAGTACGCGTTTTCCATGCCGTCTGCGGCGTAAACACCTTAta                                                                                     >  2:1893991/1‑149 (MQ=255)
                                                                   cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGc                                                                                 >  2:144563/1‑150 (MQ=255)
                                                                   cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCACCATTTGCTGGCCCCAGAATGATAACAAAGTAAGCGTTATCCATCCCGGATGCGGCGTAAACCCCTTATCCCGc                                                                                 >  2:191145/1‑150 (MQ=255)
                                                                     ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCt                                                                               <  1:129714/150‑1 (MQ=255)
                                                                        ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACt                                                                            >  2:863032/1‑150 (MQ=255)
                                                                        ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACt                                                                            <  2:1088819/150‑1 (MQ=255)
                                                                         cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTc                                                                           <  2:314326/150‑1 (MQ=255)
                                                                          gTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCACAAGAAAAAATCAGGCCACCATTTGCTGGCCCCAGATAGATAACAAAGTACGCGCTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCCCCTACTCg                                                                          <  2:529713/150‑1 (MQ=255)
                                                                            cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAt                                                                        >  2:269203/1‑150 (MQ=255)
                                                                            cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAt                                                                        >  1:838568/1‑150 (MQ=255)
                                                                               tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGt                                                                     >  1:1621845/1‑150 (MQ=255)
                                                                               tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGt                                                                     >  1:535917/1‑150 (MQ=255)
                                                                               tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGt                                                                     >  1:1177856/1‑150 (MQ=255)
                                                                                gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTg                                                                    <  2:1873187/150‑1 (MQ=255)
                                                                                gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCACGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTg                                                                    <  2:323703/150‑1 (MQ=255)
                                                                                 aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGc                                                                   <  2:904276/150‑1 (MQ=255)
                                                                                  aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCa                                                                  <  2:823770/150‑1 (MQ=255)
                                                                                  aaGTGCGGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAAACAGGCCAGAATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCa                                                                  <  2:299250/150‑1 (MQ=255)
                                                                                       ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCttt                                                             >  1:983496/1‑150 (MQ=255)
                                                                                       ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCttt                                                             >  1:1939363/1‑150 (MQ=255)
                                                                                        tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTc                                                            <  1:266990/150‑1 (MQ=255)
                                                                                        tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCGCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCCTGCCGGATGCGGCGTAAACCCCTTATCCCGCCTAGTCGCTCGTGCATCTTTc                                                            >  2:48315/1‑150 (MQ=255)
                                                                                         gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTcc                                                           <  1:1727594/150‑1 (MQ=255)
                                                                                           cgccAGCAGCATTTAGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGCCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTCTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAg                                                         <  2:1288700/150‑1 (MQ=255)
                                                                                            gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAgg                                                        <  2:1811384/150‑1 (MQ=255)
                                                                                            gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGAGTTTTCCATGCCCGATGCGGCGTAAACACCTTATCCCGCATACTCGATCGTGAATCTTTCCAgg                                                        >  1:406618/1‑150 (MQ=255)
                                                                                             ccAGCAGCATTTTGCGCAGCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGc                                                       <  1:1079675/150‑1 (MQ=255)
                                                                                                      ttttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGc                                              <  1:1245481/150‑1 (MQ=255)
                                                                                                       tttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCg                                             >  1:1178702/1‑150 (MQ=255)
                                                                                                        ttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCCGGCCTGATAAGCGt                                            >  2:1291736/1‑150 (MQ=255)
                                                                                                        ttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGt                                            <  1:1869708/150‑1 (MQ=255)
                                                                                                              atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATATCAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTTCACTTTCGTGAATCTTTCCAGGCCTGATAAGCGTAgcgaa                                      >  2:1221148/1‑148 (MQ=39)
                                                                                                              atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCa                                      >  2:689989/1‑150 (MQ=255)
                                                                                                              atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATATGCTGGCCCCAGATTGATAAAAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAAAACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAACCGTAACGCa                                      >  2:1331742/1‑150 (MQ=255)
                                                                                                                catAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATc                                    >  1:401923/1‑150 (MQ=255)
                                                                                                                    gTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGc                                >  2:5993/1‑150 (MQ=255)
                                                                                                                        tCCCTCAAGAAAAAATCCGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCCTCAggacgtt                            >  1:1080957/1‑145 (MQ=255)
                                                                                                                         cccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGttt                           >  2:1503531/1‑150 (MQ=255)
                                                                                                                         cccTCAAGAAAAAATCAGGCCAGCATATGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGttt                           <  2:946274/150‑1 (MQ=255)
                                                                                                                          ccTCAAGAAAAACTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt                          <  1:59786/150‑1 (MQ=255)
                                                                                                                            tCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGc                        <  2:1704879/150‑1 (MQ=255)
                                                                                                                             cAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCa                       <  2:150006/150‑1 (MQ=255)
                                                                                                                               aGAAAAAATCAGGCCAGCATTTGGTGGCCCCAGATTGATAACAAAGTACGCGGTTTCCATGCCGGATGCGGCGGAAACACCTTATCCCGCCTACTCGAGCGTGCATCTTTCCCGGCCTGATTAGCGTAGCGCATCAGGCAGTTTTGCAtt                     >  1:363522/1‑150 (MQ=255)
                                                                                                                               aGAAAAAATCAGGCCAGCATTTGCTGGCCGCAGATTGATAACTAAGGACGCGTTTTCCATGCCGGATGCGGCGTAAACAGCTTATCCCGCCTACTCGATCGTGCTTATTTCCAGGCATGATAAGCGTAGCTCATAATGCAGTTTTGAAtt                     >  1:1371545/1‑150 (MQ=255)
                                                                                                                                gAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAttt                    >  1:1013114/1‑150 (MQ=255)
                                                                                                                                gAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAttt                    >  1:546129/1‑150 (MQ=255)
                                                                                                                                gAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAttt                    >  2:40646/1‑150 (MQ=255)
                                                                                                                                  aaaaaTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGt                  >  2:1046814/1‑150 (MQ=255)
                                                                                                                                    atctggggCCAGCAAATGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCa                >  1:848681/7‑150 (MQ=255)
                                                                                                                                       tggggCCAGCAAATGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCg             >  2:409485/4‑150 (MQ=255)
                                                                                                                                        ggggCCAGCAAATGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGc            >  2:707345/3‑150 (MQ=255)
                                                                                                                                         aGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGcc           <  2:450120/150‑1 (MQ=255)
                                                                                                                                         aGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGcc           <  2:266842/150‑1 (MQ=255)
                                                                                                                                          ggCCAGCTTTTGCTTGCCCCAGATTGATAACAAAGTACGCTTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCTCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGccc          <  2:1490880/150‑1 (MQ=255)
                                                                                                                                          ggCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGccc          <  2:1175040/150‑1 (MQ=255)
                                                                                                                                          ggCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGGTTTGCATTTGTCATCGccc          >  1:170482/1‑150 (MQ=255)
                                                                                                                                          ggCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGGTTTCCATGCCGGATGCGGCGTAACCACCTTATCCCGCCTACTCGAGCGTGCATCTTTCCAGGCCTGATAAGCGTCGCGCATCAGGCAGTTTTGCATTTGTCATCGcgc          >  1:1741807/1‑148 (MQ=255)
                                                                                                                                            ccAGCATTTGCTGGCCCCAGATTGATAACAAAGTAGGCGTTTTCCATGCCGGATGCGTCGTTAACACCTTATCCCGCCTACTCGATCGAGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGt        <  1:350008/150‑1 (MQ=255)
                                                                                                                                            ccAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGt        >  1:639712/1‑150 (MQ=255)
                                                                                                                                            ccAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGt        >  2:1846675/1‑150 (MQ=255)
                                                                                                                                            ccAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGt        >  2:1721787/1‑150 (MQ=255)
                                                                                                                                            ccAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCAAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGt        >  1:1190642/1‑150 (MQ=255)
                                                                                                                                            ccAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGAGCAACTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGt        >  2:74236/1‑150 (MQ=255)
                                                                                                                                                  tttGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGCtt  <  2:720684/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGCTT  >  NC_000913/3101509‑3101804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑