Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,862 A→T 7.8% L142H (CTT→CAT) ‡ hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,8620AT 7.8% 391.7 / 16.9 146L142H (CTT→CAT) ‡hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base A (65/68);  new base T (3/9);  total (68/78)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

Only 100 of 148 total aligned reads displayed.

GTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGAT  >  NC_000913/3140713‑3141009
                                                                                                                                                     |                                                                                                                                                   
gTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTAGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCaa                                                                                                                                                     >  1:159847/1‑150 (MQ=255)
gTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCATGTACTTGCTCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCCTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCaa                                                                                                                                                     >  2:1973942/1‑150 (MQ=255)
  gCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgc                                                                                                                                                   >  2:1405963/1‑150 (MQ=255)
   cATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATACACGCAGCGCAGCCAGAACCTGCTCAAgcg                                                                                                                                                  >  1:1871282/1‑150 (MQ=255)
   cATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTACTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcg                                                                                                                                                  <  2:1493776/150‑1 (MQ=255)
       gAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGACCCTGCTCAAGCGCAgg                                                                                                                                              >  1:171495/1‑150 (MQ=255)
          cGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCAGAACCTGCGCTTGAGCAGGTTct                                                                                                                                          <  2:1036003/150‑2 (MQ=255)
           gCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTct                                                                                                                                          <  2:1616418/150‑2 (MQ=255)
           gCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTct                                                                                                                                          <  1:421949/150‑2 (MQ=255)
            ccGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCaa                                                                                                                                         <  2:694344/150‑1 (MQ=255)
            ccGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctg                                                                                                                                         <  2:959770/150‑3 (MQ=255)
            ccGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctg                                                                                                                                         <  1:1898626/150‑3 (MQ=255)
             cGGGCAATTTCTTCTAACGGTGCCTGTACTTGCGCCTTCGGGGAGTTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCGAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAAt                                                                                                                                        <  2:457505/150‑1 (MQ=255)
             cGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCTGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAAt                                                                                                                                        <  1:10046/150‑1 (MQ=255)
             cGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctgg                                                                                                                                        <  2:1928071/150‑4 (MQ=255)
              gggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATc                                                                                                                                       <  1:615339/150‑1 (MQ=255)
              gggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGGCTGGAAACCTGCTAGCGCTTCAGTCCTGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctggc                                                                                                                                       <  1:1484840/150‑5 (MQ=255)
                 cAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGATACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCAtt                                                                                                                                    <  1:569517/150‑1 (MQ=255)
                 cAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCAtt                                                                                                                                    <  2:795764/150‑1 (MQ=255)
                  aaTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTg                                                                                                                                   <  2:773557/150‑1 (MQ=255)
                  aaTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTg                                                                                                                                   <  2:1037519/150‑1 (MQ=255)
                    tttCTTCACCCGCTGCCTGTACTTGCGCCTTCGGGGAGGGCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTGCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCt                                                                                                                                 <  2:282324/150‑1 (MQ=255)
                      tcttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTc                                                                                                                               <  2:1292267/150‑1 (MQ=255)
                       cttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                              >  2:1252170/1‑150 (MQ=255)
                        ttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTccc                                                                                                                             >  2:1447307/1‑148 (MQ=255)
                        ttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGGCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCaa                                                                                                                             <  1:2003929/150‑1 (MQ=255)
                         tcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAAc                                                                                                                            >  2:1180486/1‑150 (MQ=255)
                            atcgcgGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATTAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGt                                                                                                                         <  1:1920268/148‑1 (MQ=255)
                             aCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTc                                                                                                                        <  2:590330/150‑1 (MQ=255)
                                 tGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCTTGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCg                                                                                                                    <  2:1113598/150‑1 (MQ=255)
                                    cTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTa                                                                                                                 >  1:1357344/1‑150 (MQ=255)
                                      gTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAg                                                                                                               <  1:609969/150‑1 (MQ=255)
                                        aCTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGcc                                                                                                             <  1:1488958/150‑1 (MQ=255)
                                         cTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCg                                                                                                            <  2:1472760/150‑1 (MQ=255)
                                           tGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAt                                                                                                          >  2:1178323/1‑150 (MQ=255)
                                             cgcCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTACACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgt                                                                                                        >  2:986454/1‑150 (MQ=255)
                                                 ttCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTgg                                                                                                    <  2:1306992/150‑1 (MQ=255)
                                                         ggTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCg                                                                                            >  2:143600/1‑150 (MQ=255)
                                                         ggTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCg                                                                                            <  1:1263047/150‑1 (MQ=255)
                                                         ggTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCg                                                                                            >  1:745231/1‑150 (MQ=255)
                                                            cTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAtt                                                                                         >  1:849070/1‑150 (MQ=255)
                                                              ggAAAGCTGCTATCTCTTCAGTCATGAATCGCCGCCCGTGGGATGGCTTCCCCGCCAGATTCACGCAGCGCAGCCAGACCCTGCTCCAGCGCAGGTTCAATCATAGCTTCAACCGTCGGCGTTAAGCCGATATGTGGATCCAGCTATTcc                                                                                       >  2:90783/1‑150 (MQ=255)
                                                              ggAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCCGCGAttgc                                                                                       >  1:378252/1‑148 (MQ=255)
                                                                 aaCCTGCTATCTCTTCAGTCATGACTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCggg                                                                                    <  2:724674/150‑1 (MQ=255)
                                                                     tGCTATCTCTTCCGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                                                                <  1:1329836/150‑1 (MQ=255)
                                                                     tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                                                                >  1:40037/1‑150 (MQ=255)
                                                                           ctctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCgc                                                                          >  2:1460942/1‑149 (MQ=255)
                                                                             ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                                                                        >  1:554230/1‑150 (MQ=255)
                                                                             ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                                                                        >  2:1875880/1‑150 (MQ=255)
                                                                                 actCATGAATCGCCTCCCGTGCGATGGCCGCCACGCCAGAATCACGCAGCCCAGCCACAACCTGCTCACGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggg                                                                    <  2:1338091/148‑1 (MQ=255)
                                                                                 aGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggg                                                                    <  1:1785910/150‑1 (MQ=255)
                                                                                 aGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAcgg                                                                    >  2:104133/1‑150 (MQ=255)
                                                                                  gTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGt                                                                   >  2:443373/1‑150 (MQ=255)
                                                                                  gTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGt                                                                   >  1:1413944/1‑150 (MQ=255)
                                                                                  gTCAGGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGAGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGt                                                                   <  2:793263/150‑1 (MQ=255)
                                                                                   tCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTc                                                                  <  2:422688/150‑1 (MQ=255)
                                                                                    cATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCa                                                                 <  1:1405963/150‑1 (MQ=255)
                                                                                     aTGAATCGCCTCCCGTGGGATGGCTTCCACTCCAGATTCTCGCAGCGCAGCCAGAACCTGCTCAAGCTCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAg                                                                <  1:67092/150‑1 (MQ=255)
                                                                                     aTGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAg                                                                <  1:1030728/150‑1 (MQ=255)
                                                                                      tGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTGCAGCGATTCGGGGATCACGCCGACCAGGGTCAGc                                                               >  1:324168/1‑150 (MQ=255)
                                                                                       gAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt                                                              >  2:1361457/1‑150 (MQ=255)
                                                                                        aaTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGCCCAGGGTCAGCtt                                                             >  2:1514832/1‑150 (MQ=255)
                                                                                        aaTCGCCTCCCGTGGGATGGATTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCATTCATTTATTCAAACGTCGGCGTTAAGCCGAGGTGTGGCTCCAGCGATTCGGGGATCACGCCGGCCAGGGTCAGCtt                                                             >  2:777766/1‑150 (MQ=255)
                                                                                           cGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGTCGTTAAGCAGATGTGTGGCTCCAGCGATTCCGGGATCACGCAGACCAGGGTCAGCttttt                                                          >  2:541641/1‑150 (MQ=255)
                                                                                           cGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCttttt                                                          >  2:1193788/1‑150 (MQ=255)
                                                                                            gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTc                                                         >  1:1061577/1‑150 (MQ=255)
                                                                                            gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTc                                                         >  2:21038/1‑150 (MQ=255)
                                                                                             gcTCCGGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCATGCGCAGGTTCAATCATTGCTTCAACGGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCg                                                        <  1:1693825/149‑1 (MQ=255)
                                                                                             ccTCCCTTGGGATGGCTTCCACGCCAGATTCACGCCGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTCCGTCCACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGCTCACGCCGACCAGGGTCAGCTTTTTCg                                                        <  2:1191782/150‑1 (MQ=255)
                                                                                             ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCg                                                        <  2:681611/150‑1 (MQ=255)
                                                                                             ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCg                                                        <  2:192869/150‑1 (MQ=255)
                                                                                              cTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCgg                                                       >  1:1856250/1‑150 (MQ=255)
                                                                                                cccGTGGTATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                     <  1:303103/150‑1 (MQ=255)
                                                                                                cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                     >  2:399930/1‑150 (MQ=255)
                                                                                                   gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACt                                                  >  1:1905602/1‑150 (MQ=255)
                                                                                                     gggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCg                                                >  1:89953/1‑150 (MQ=255)
                                                                                                         tGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCgg                                            <  1:510700/150‑1 (MQ=255)
                                                                                                            cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTTAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                         >  1:225353/1‑150 (MQ=255)
                                                                                                            cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                         >  1:1545536/1‑150 (MQ=255)
                                                                                                            cTTCCACGCCAGATTCACGCAGCGCAGACAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGTCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTgt                                         <  2:745231/150‑2 (MQ=255)
                                                                                                                 aCGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAgcgc                                    >  2:494236/1‑150 (MQ=255)
                                                                                                                     cAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAggg                                <  1:190848/150‑1 (MQ=255)
                                                                                                                       gATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGGCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGAGCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGcc                              <  2:108562/150‑1 (MQ=255)
                                                                                                                             cgcagcgcagCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAg                        >  1:1922849/1‑150 (MQ=255)
                                                                                                                              gcagcgcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGa                       <  1:677813/150‑1 (MQ=255)
                                                                                                                                   gcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCg                  <  2:1871282/150‑1 (MQ=255)
                                                                                                                                   gcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCg                  >  1:1691206/1‑150 (MQ=255)
                                                                                                                                   gcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCg                  >  2:1667232/1‑150 (MQ=255)
                                                                                                                                       ccAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAACCTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccc              >  1:1905975/1‑149 (MQ=255)
                                                                                                                                       ccAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTTAAGCGCAGGGCCGACAAGACGTCggcca              <  1:1898804/150‑1 (MQ=255)
                                                                                                                                         tgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccagg            >  2:1420559/2‑150 (MQ=255)
                                                                                                                                         aGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGCCCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCCGGGCCGACAAGACGTCggccagg            >  1:1253642/1‑150 (MQ=255)
                                                                                                                                         aGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccagg            >  1:520527/1‑150 (MQ=255)
                                                                                                                                          gAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggc           <  2:1357344/150‑1 (MQ=255)
                                                                                                                                          gAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCATCTTTTTCGGAAACTCCCCGGTGAAGCGCCGGGCCGACAAGACGTCggccaggc           >  1:1216257/1‑150 (MQ=255)
                                                                                                                                          gAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggc           >  2:575636/1‑150 (MQ=255)
                                                                                                                                           aaCCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggcc          <  1:1202430/150‑1 (MQ=255)
                                                                                                                                             ccTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCaa        <  2:903782/150‑1 (MQ=255)
                                                                                                                                                 cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACTCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg    >  1:703047/1‑150 (MQ=255)
                                                                                                                                                 cTCAAGCGCAGGTTCAATCAATGCTTCAACCGTCGGCGTTAAGCCGATGTGAGGCACCAGCGATACCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGACACTCGCCGCTGAAGCGCAGGGCAGACAAGACGTCGGCCAGGCCCAGcgc    >  1:250865/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGAT  >  NC_000913/3140713‑3141009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: