Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,179,613 T→G 5.4% intergenic (+17/‑131) tolC → / → ygiB transport channel/DUF1190 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,179,6130TG 5.4% 374.2 / 4.1 132intergenic (+17/‑131)tolC/ygiBtransport channel/DUF1190 family protein
Reads supporting (aligned to +/- strand):  ref base T (56/66);  new base G (2/7);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

AACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCA  >  NC_000913/3179465‑3179762
                                                                                                                                                    |                                                                                                                                                     
aaCGTTTCACCGCAAACGCCGGTACTGTATGCGATTTCTGATGGTTATGCGCCCGATAGCCCGGCACCAGGCGTTAAGCAAACATCCGCACGCACTACGACCAGTAACGGTCATAACCCTTTCCGAAACAGATCACGACGACTGGGGTTc                                                                                                                                                      <  2:30452/150‑1 (MQ=255)
   gTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGc                                                                                                                                                   >  2:475709/1‑150 (MQ=255)
      gCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGcccc                                                                                                                                                <  2:1845357/150‑1 (MQ=255)
      gCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGcccc                                                                                                                                                >  1:68734/1‑150 (MQ=255)
       cACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAACCATCCGCACGCACTACCCCCAGTAACGGTCATACACCTTTCCGTTACTGATGACGCCGCCGGGGCATCGGCCCCg                                                                                                                                               >  1:988788/1‑150 (MQ=255)
       cACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCg                                                                                                                                               <  2:448536/150‑1 (MQ=255)
         ccGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTc                                                                                                                                             <  1:449568/150‑1 (MQ=255)
         ccGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTc                                                                                                                                             <  1:1275435/150‑1 (MQ=255)
          cGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCCGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTcg                                                                                                                                            >  1:1625261/1‑149 (MQ=255)
             aaaCGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCTGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCCGAAGCCCCGTcgtcg                                                                                                                                         <  1:557882/150‑5 (MQ=255)
             aaaCGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGAAACGGTCATAACCCTTTCCGAAACTGATGACGACGACGGGGCCGAAGCCCCGTcgtcg                                                                                                                                         <  1:429669/150‑5 (MQ=255)
               aCGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACg                                                                                                                                       <  2:714418/150‑1 (MQ=255)
                  ccGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCGTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTaa                                                                                                                                    >  1:1354136/1‑150 (MQ=255)
                    ggAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAgg                                                                                                                                  >  2:1123412/1‑150 (MQ=255)
                     gAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGc                                                                                                                                 <  1:509695/150‑1 (MQ=255)
                       aCAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCaa                                                                                                                               <  1:1031270/150‑1 (MQ=255)
                          gAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGCACGTAAGGCAACGt                                                                                                                            >  2:1054849/1‑150 (MQ=255)
                             tGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGACTTCGGCCCCGTCTGAACGTAAGGCAACGTaaa                                                                                                                         <  2:672447/150‑1 (MQ=255)
                              gCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAg                                                                                                                        <  1:1290748/150‑1 (MQ=255)
                                 aTTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATa                                                                                                                     >  2:917236/1‑150 (MQ=255)
                                   tGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACg                                                                                                                   >  1:506954/1‑150 (MQ=255)
                                     cTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACggg                                                                                                                 <  2:814160/150‑1 (MQ=255)
                                     cTGATGGTTATGCGCCTGATAGCCCGGCACCAGGCGTTCAGCTAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTATCCGTAACTGAGGACGACGACGGGGCTGCGGCCCCGTCTGCACGTAAGCCAGCGTAAAGCTACggg                                                                                                                 >  1:291315/1‑150 (MQ=255)
                                     cTGATGGTTATGCGACTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGGCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACggg                                                                                                                 <  2:1101311/150‑1 (MQ=255)
                                     cTGATGGGTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGTCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACggg                                                                                                                 <  1:759508/150‑1 (MQ=255)
                                      tGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGt                                                                                                                >  1:1196458/1‑150 (MQ=255)
                                       gATGGTTATGCGCCTGATAGCCCGGCACCAGGCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGtt                                                                                                               <  2:1877632/150‑1 (MQ=255)
                                        aTGGTTATGCGCCTGATAGCCCGGCACCAGGCGTGCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGGTa                                                                                                              <  1:97055/150‑1 (MQ=255)
                                          ggTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATc                                                                                                            <  2:1151036/150‑1 (MQ=255)
                                               tGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCg                                                                                                       <  1:1064609/150‑1 (MQ=255)
                                                gcgcCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGc                                                                                                      >  2:1306019/1‑150 (MQ=255)
                                                    cTGATAACCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCAttc                                                                                                  <  1:1597325/150‑1 (MQ=255)
                                                     tGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCAttct                                                                                                 <  1:694583/150‑1 (MQ=255)
                                                      gATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGGAACTGATGACGACGACGGGGCTTCGGCCCCGTGGGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCAgtctt                                                                                                >  2:1275074/1‑150 (MQ=255)
                                                        tAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTACGGCCCCGTCTGAACGTAAGGCAACGTAAAGCTACGGGTTATCTGCCGCAttcttgc                                                                                              >  2:1498326/1‑148 (MQ=255)
                                                         aGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTTAACGTAAGGCAACGTAAAGATACGGGTTATCTGCAGCATGCTTccc                                                                                             >  2:1739600/1‑150 (MQ=255)
                                                         aGCCCGGCACCAGTCGTTCAGCAAACATCAGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTccc                                                                                             <  2:799819/150‑1 (MQ=255)
                                                          gCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTcccc                                                                                            <  1:479182/150‑1 (MQ=255)
                                                           cccGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTccccc                                                                                           <  2:1949994/150‑1 (MQ=255)
                                                           cccGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTccccc                                                                                           <  2:576707/150‑1 (MQ=255)
                                                            ccGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTGCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTcccccg                                                                                          >  2:148325/1‑149 (MQ=255)
                                                            ccGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCGTCGGCCCCGTCTGAACGTTAGGCAACGTAAAGATTCGGGTTATCTGCCGCATTCTTccccca                                                                                          >  2:1651750/1‑149 (MQ=255)
                                                                     gTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTc                                                                                 <  1:1194286/150‑1 (MQ=255)
                                                                      tCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCa                                                                                <  2:716975/150‑1 (MQ=255)
                                                                      tCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCa                                                                                <  1:1166848/150‑1 (MQ=255)
                                                                        gTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAAt                                                                              <  2:1548677/150‑1 (MQ=255)
                                                                          tCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTGCCAttt                                                                            >  2:803457/1‑150 (MQ=255)
                                                                           cAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATACCCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCGGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCCTTCTTCCCCCGTCTCGCTTCAATTTc                                                                           >  1:1832730/1‑150 (MQ=255)
                                                                            aGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCg                                                                          <  2:1389803/150‑1 (MQ=255)
                                                                            aGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCg                                                                          <  2:1723577/150‑1 (MQ=255)
                                                                            aGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACCGGGCTTCGGCCCCGGGTGAACGTTAGGCAACGTAAAGATACGGGTTATGTTCCGCATTCATGCCCCGTCTCGGTTGAAtttag                                                                          >  2:1825593/1‑148 (MQ=255)
                                                                             gCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTTTCTGCCGCATTAATACCCCTTCTCGCTTCAATTTCGa                                                                         >  1:1754309/1‑150 (MQ=255)
                                                                             gCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGa                                                                         >  2:493167/1‑150 (MQ=255)
                                                                             gCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATCACGACGACGGCGCTTCGGCCCCGACTGAACGAAAGGCAACGTAAAGATACGGGTTAACTGCCGCAATCTTCCCCCTTCTCGCTTCAATTTCGa                                                                         >  1:385495/1‑150 (MQ=255)
                                                                              cAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATTCGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGAc                                                                        >  1:1960435/1‑150 (MQ=255)
                                                                               aaaCATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGAcc                                                                       <  2:1494381/150‑1 (MQ=255)
                                                                                  cATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGCCCAGc                                                                    >  1:1063951/1‑150 (MQ=255)
                                                                                  cATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGc                                                                    >  2:926939/1‑150 (MQ=255)
                                                                                      cgcacgcaCTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCCTc                                                                >  1:649860/1‑150 (MQ=255)
                                                                                      cgcacgcaCTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATc                                                                >  2:1749860/1‑150 (MQ=255)
                                                                                         acgcacTACCACCAGTAACGGTCATAACCCTTTCCGTAACAGATGACGTCGTCGGGGCTTCGGCAGCGTCTGATAGTAAGGCTACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGAACAGCCATCctc                                                             <  1:283211/150‑1 (MQ=255)
                                                                                          cgcacTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCctcctcg                                                            >  1:360112/1‑149 (MQ=255)
                                                                                          cgcacTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCCTCctct                                                            >  1:975412/1‑150 (MQ=255)
                                                                                            cacTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCCTTCTTCCCCCTTCTCGCTTCAATTTAGACCAGCCATCCTCGAt                                                          >  1:1819283/1‑150 (MQ=255)
                                                                                            cacTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCCGCCCTCCTGTAt                                                          >  1:1128999/1‑150 (MQ=255)
                                                                                            cacTACCACCAGTAACGGTCATAACCCTGTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGGCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCGCCCTTCGCGCGGCCATGTCGACCAGCCATCCTCAAt                                                          >  2:117499/1‑150 (MQ=255)
                                                                                             acTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTAtt                                                         >  1:661223/1‑150 (MQ=255)
                                                                                              cTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTc                                                        <  2:1180316/150‑1 (MQ=255)
                                                                                              cTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTc                                                        <  2:631513/150‑1 (MQ=255)
                                                                                               aaccaccAGTAACGGTCATAACACTTTCAGTAAGTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCt                                                       <  2:686956/149‑1 (MQ=255)
                                                                                                  caccaGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGAt                                                    <  1:575106/150‑1 (MQ=255)
                                                                                                   accaGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATg                                                   <  1:1306019/150‑1 (MQ=255)
                                                                                                   accaGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATg                                                   <  1:917236/150‑1 (MQ=255)
                                                                                                   accaGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGGCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCGTCGCGCTTCAATTTCGACCAGCCATCCTCGTTTCTGATg                                                   >  1:1021999/1‑150 (MQ=255)
                                                                                                    ccaGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATgg                                                  <  1:1564751/150‑1 (MQ=255)
                                                                                                     caGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACTTAAGGGAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATggg                                                 <  2:1986471/150‑1 (MQ=255)
                                                                                                                aTAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACt                                      <  1:1734172/150‑1 (MQ=255)
                                                                                                                 acaCCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTg                                     >  2:139591/3‑150 (MQ=255)
                                                                                                                  aaCCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTgg                                    <  2:1216390/150‑1 (MQ=255)
                                                                                                                     ccTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTcc                                 >  1:4380/1‑150 (MQ=255)
                                                                                                                      cTTTCCGTAACTGATGACGAGGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCg                               <  2:205583/150‑1 (MQ=255)
                                                                                                                          ccGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGaa                            >  1:1144278/1‑150 (MQ=255)
                                                                                                                           cGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTATAGATACGGGTTATCTGCCGCATTCTTCCCCCGTCTCGCTTCAATTTCGACCCGCCATCCGCGATTCTGATGGGTTTTTGCCCCTGGTCCCGGaac                           >  2:966404/1‑149 (MQ=255)
                                                                                                                           cGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAg                           <  1:392042/150‑1 (MQ=255)
                                                                                                                           cGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAg                           <  2:1196458/150‑1 (MQ=255)
                                                                                                                           cGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAg                           <  2:156752/150‑1 (MQ=255)
                                                                                                                           cGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCAGTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGaac                           >  1:249479/1‑149 (MQ=255)
                                                                                                                           cGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCAGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCATCCGCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAg                           >  2:114361/1‑150 (MQ=255)
                                                                                                                             tAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGAc                         <  1:1529811/150‑1 (MQ=255)
                                                                                                                                 tgatgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACaaaa                     <  1:962800/150‑1 (MQ=255)
                                                                                                                                 tgatgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACaaaa                     >  2:1332515/1‑150 (MQ=255)
                                                                                                                                  gatgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATATGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACaaaaa                    <  2:388181/150‑1 (MQ=255)
                                                                                                                                  gatgaCGACGACGGGGCTGCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCAATTCTGATGGGTATTTACCACTGGTCCCGGAAGACaaaaa                    <  2:325584/150‑1 (MQ=255)
                                                                                                                                   atgaCGACGACGGGGCTTCGGCCCCGTCTGCACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTGTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAAt                   >  1:847065/1‑150 (MQ=255)
                                                                                                                                      acgacgacgGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGaa                <  1:16818/150‑1 (MQ=255)
                                                                                                                                        gacgacgGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTTCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAAc              <  1:804618/150‑1 (MQ=255)
                                                                                                                                        gacgacgGGGCTGCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAAc              <  1:811560/150‑1 (MQ=255)
                                                                                                                                          cgacgGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACgg            <  1:1089446/150‑1 (MQ=255)
                                                                                                                                             cgGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACAACTGGTCCCGGAAGACAAAAATGAAACGGACa         >  2:101974/1‑150 (MQ=255)
                                                                                                                                                    tCGGCCCCGTCTGAACGTAAGGCAACGTAAAGTTACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCa  <  1:1781888/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCACGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCA  >  NC_000913/3179465‑3179762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: