Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 318,649 T→C 5.3% intergenic (‑82/+27) rclB ← / ← rclA reactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913318,6490TC 5.3% 514.1 / 13.2 169intergenic (‑82/+27)rclB/rclAreactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (83/76);  new base C (2/7);  total (86/83)
Fisher's exact test for biased strand distribution p-value = 9.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

Only 100 of 173 total aligned reads displayed.

CTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCA  >  NC_000913/318501‑318797
                                                                                                                                                    |                                                                                                                                                    
cTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAata                                                                                                                                                     >  1:389856/1‑150 (MQ=255)
 tGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAatat                                                                                                                                                    <  2:1001148/150‑1 (MQ=255)
  gCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCAAGAATAtt                                                                                                                                                   >  2:1196776/1‑150 (MQ=255)
   cATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAttt                                                                                                                                                  >  1:273187/1‑150 (MQ=255)
   aaTTGTTGGAAAATGACATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAttt                                                                                                                                                  <  1:1515476/149‑1 (MQ=255)
    aTTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTg                                                                                                                                                 <  1:1035899/150‑1 (MQ=255)
    aTTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTg                                                                                                                                                 <  2:1874836/150‑1 (MQ=255)
    aTTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTg                                                                                                                                                 <  1:1579937/150‑1 (MQ=255)
       ggtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCAAGAATATTTGTTc                                                                                                                                              >  2:1776924/1‑150 (MQ=255)
        gtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCt                                                                                                                                             >  1:1144809/1‑150 (MQ=255)
        gtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCt                                                                                                                                             >  2:497434/1‑150 (MQ=255)
          ggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAGCAGATTTTTTAAACATATAAATTACCAGCAATGAAGTGATTAAGAAGTTATATATTAACAGAGAATTAATAAGATAATTTACTCTTAGACGCCAGAAGATTTGTTCTgg                                                                                                                                           >  2:1481590/1‑150 (MQ=255)
          ggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTgg                                                                                                                                           >  2:1022252/1‑150 (MQ=255)
          ggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTgg                                                                                                                                           <  2:1668528/150‑1 (MQ=255)
           gAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGc                                                                                                                                          <  1:869372/150‑1 (MQ=255)
            aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCg                                                                                                                                         <  1:502995/150‑1 (MQ=255)
            aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCg                                                                                                                                         <  1:602898/150‑1 (MQ=255)
             aaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTATTAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCGt                                                                                                                                        <  1:1716939/150‑1 (MQ=255)
              aaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAAAACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCGTc                                                                                                                                       <  2:13762/150‑1 (MQ=255)
                     tAACGCCAGATAATAGATTTGCAAATAAAACAGATTTTTAATACATTTAAAATACCATCAAAGAAGTGATTAAGAAGTTATTGCGTAACAGAGATTTAATAAGATTCTTTTCTCTGAGACGCCAGATTATTTGTTCTGGCTTCTGAtttt                                                                                                                                >  2:438130/1‑150 (MQ=255)
                      aaCGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTg                                                                                                                               <  1:1034758/150‑1 (MQ=255)
                       aCGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGa                                                                                                                              <  2:660083/150‑1 (MQ=255)
                          ccAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTTACAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAAACGCCAGAATATTTTTTCTGGCGTCTGATTTTGAGttt                                                                                                                          >  2:781028/1‑150 (MQ=255)
                           cAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGttt                                                                                                                          >  2:1314873/1‑150 (MQ=255)
                                aataGTGTTTCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATTAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTg                                                                                                                     <  1:1672072/150‑1 (MQ=255)
                                  taGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGag                                                                                                                   >  2:301555/1‑149 (MQ=255)
                                   aGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACt                                                                                                                  <  1:864282/150‑1 (MQ=255)
                                   aGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACt                                                                                                                  <  1:533857/150‑1 (MQ=255)
                                      gtTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACATAGATTTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAAt                                                                                                               <  2:506790/150‑1 (MQ=255)
                                         tcAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTTATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTTTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaa                                                                                                            <  1:713661/149‑1 (MQ=255)
                                         gCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTGTTCTCTGATACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaa                                                                                                            >  1:215510/1‑150 (MQ=255)
                                           aaaTAAAACAGATTTTTTAAACATATAAAATACCTTCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaaaa                                                                                                          >  1:1853177/1‑150 (MQ=255)
                                           aaaTAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaaaa                                                                                                          >  2:1667859/1‑150 (MQ=255)
                                           aaaTAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaaaa                                                                                                          >  1:568769/1‑150 (MQ=255)
                                            aaTAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAAt                                                                                                         >  1:1076826/1‑150 (MQ=255)
                                            aaTAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAAt                                                                                                         >  1:575902/1‑150 (MQ=255)
                                             aTAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATa                                                                                                        >  2:1640338/1‑150 (MQ=255)
                                               aaaaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGa                                                                                                      >  1:1691167/1‑150 (MQ=255)
                                               aaaaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGa                                                                                                      <  2:563830/150‑1 (MQ=255)
                                               aaaaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGa                                                                                                      >  2:1088651/1‑150 (MQ=255)
                                                 aaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATc                                                                                                    <  2:784646/150‑1 (MQ=255)
                                                 aaCAGATTTTTTAAACATATAAAAAACAATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAATATAGTTTAATCTGAGACGCCAGAATATTTATTCTGGCGTCTGATTTTGATTTTATTTGACAAAAGAAAatatata                                                                                                    >  1:964455/1‑149 (MQ=255)
                                                   cAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCAt                                                                                                  >  2:402262/1‑150 (MQ=255)
                                                   cAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCAt                                                                                                  >  2:77066/1‑150 (MQ=255)
                                                   cAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCAAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCAt                                                                                                  >  1:250625/1‑150 (MQ=255)
                                                    aGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCAtt                                                                                                 <  1:623858/150‑1 (MQ=255)
                                                     taTTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTg                                                                                                <  1:1966690/149‑1 (MQ=255)
                                                           ttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGAt                                                                                          >  1:1911952/1‑150 (MQ=255)
                                                            tAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGAtt                                                                                         >  1:1638651/1‑150 (MQ=255)
                                                             aaaCATATAAAATACCATCAATGAAGTTATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTc                                                                                        <  1:492222/150‑1 (MQ=255)
                                                                cATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGATACGCCAGAATATTTGTTCTGGCGTCTGATTTTTAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCt                                                                                     <  1:129723/150‑1 (MQ=255)
                                                                aatatAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCt                                                                                     <  1:196734/149‑1 (MQ=255)
                                                                    taAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATc                                                                                 <  2:1323197/150‑1 (MQ=255)
                                                                     aaaaTACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCg                                                                                <  2:1838930/150‑1 (MQ=255)
                                                                         tACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                            <  2:639976/150‑1 (MQ=255)
                                                                         tACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                            <  1:749172/150‑1 (MQ=255)
                                                                           ccATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACTGAt                                                                          >  2:1696083/1‑150 (MQ=255)
                                                                            cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                         >  2:1808435/1‑150 (MQ=255)
                                                                                aaTGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTa                                                                     <  1:1336494/150‑1 (MQ=255)
                                                                                   gAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAat                                                                  <  2:657367/150‑1 (MQ=255)
                                                                                   gAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAat                                                                  >  2:357035/1‑150 (MQ=255)
                                                                                   gAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAat                                                                  >  1:1387220/1‑150 (MQ=255)
                                                                                            aagaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcg                                                         <  2:1122677/150‑1 (MQ=255)
                                                                                            aagaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcg                                                         <  2:20200/150‑1 (MQ=255)
                                                                                            aagaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcg                                                         <  2:389856/150‑1 (MQ=255)
                                                                                               aagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATTAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGt                                                      <  2:992652/150‑1 (MQ=255)
                                                                                                     taGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATg                                                <  1:594319/150‑1 (MQ=255)
                                                                                                       tagTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCt                                              <  1:918128/149‑1 (MQ=255)
                                                                                                        attAACTGAGACTTAATAAGATGATTTTCTCTGAGACGCCAGAATTTTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCta                                             <  1:182259/148‑1 (MQ=255)
                                                                                                         gTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCtat                                            >  1:331511/1‑150 (MQ=255)
                                                                                                          tAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCtata                                           >  2:405975/1‑150 (MQ=255)
                                                                                                            aCAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGAAGGATGAGTAAATATCTGATCGCGTAATATGCTATAAg                                         >  1:1261401/1‑150 (MQ=255)
                                                                                                             cAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAgg                                        >  2:719329/1‑150 (MQ=255)
                                                                                                                agaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAg                                     >  2:207762/1‑150 (MQ=255)
                                                                                                                 gaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATATATCATTGAGTGATTCGCTCATAGACTGATGAGTAAATATATGATAGCGTAATATGCTATAAGTCAGc                                    >  1:235678/1‑150 (MQ=255)
                                                                                                                 gaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGc                                    >  2:1741189/1‑150 (MQ=255)
                                                                                                                     taataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCgg                                <  1:835618/150‑1 (MQ=255)
                                                                                                                     taataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCgg                                >  2:1743849/1‑150 (MQ=255)
                                                                                                                     taataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCgg                                <  1:517496/150‑1 (MQ=255)
                                                                                                                      aataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcgcg                                                       <  1:1888211/126‑1 (MQ=255)
                                                                                                                      aataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGc                               >  1:1701079/1‑150 (MQ=255)
                                                                                                                        taaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGcat                             >  2:1064946/1‑150 (MQ=255)
                                                                                                                         aaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGcatc                            >  2:1554695/1‑150 (MQ=255)
                                                                                                                            aTTCTTTTCTCTGAGATGCCAGAATATTTGTTCTGTCGTCTGATTTTGATTTTATTTGACTTATGAAAATAGATCATTGAGTGATTCTCTAATCGACGTTTGTGTAAATATCTGATCGCGTAATTTGCTATAAGGCTGCGCGGcatccat                         <  2:186172/150‑1 (MQ=255)
                                                                                                                               cTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc                      <  1:363976/150‑1 (MQ=255)
                                                                                                                               cTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc                      <  2:568769/150‑1 (MQ=255)
                                                                                                                                    ctctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCAttt                 <  2:1404073/150‑1 (MQ=255)
                                                                                                                                      ctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTc               <  1:440279/150‑1 (MQ=255)
                                                                                                                                      atcagaCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTc               >  2:1975181/4‑150 (MQ=255)
                                                                                                                                       tGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCa              >  1:1449923/1‑150 (MQ=255)
                                                                                                                                        gagaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCAc             <  1:357035/150‑1 (MQ=255)
                                                                                                                                         agaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACt            <  1:1593455/150‑1 (MQ=255)
                                                                                                                                          taCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACta           <  1:709456/149‑1 (MQ=255)
                                                                                                                                            cGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTTACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATACATCACCATTTTCACtata         >  1:670169/1‑150 (MQ=255)
                                                                                                                                             gCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTatat        >  1:600464/1‑150 (MQ=255)
                                                                                                                                              ccAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATAtt       <  1:1776924/150‑1 (MQ=255)
                                                                                                                                               cAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTg      <  2:1059330/150‑1 (MQ=255)
                                                                                                                                                  tatatTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGatc   <  2:820789/149‑1 (MQ=255)
                                                                                                                                                  aaTATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGatc   <  2:610414/150‑1 (MQ=255)
                                                                                                                                                   atatTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGatca  <  2:526914/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCA  >  NC_000913/318501‑318797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: