Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,155 A→T 5.0% K497N (AAA→AAT) ‡ fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,1550AT 5.0% 420.9 / 8.3 139K497N (AAA→AAT) ‡fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (73/58);  new base T (3/4);  total (76/63)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

Only 100 of 143 total aligned reads displayed.

CCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAG  >  NC_000913/3233007‑3233305
                                                                                                                                                    |                                                                                                                                                      
 cGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGt                                                                                                                                                      >  2:1184835/1‑150 (MQ=255)
   cgACGGCGGATCAGCTACAGGCTCTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTg                                                                                                                                                    <  1:1843214/149‑1 (MQ=255)
    gACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGc                                                                                                                                                   <  2:242308/150‑1 (MQ=255)
     aCGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGATGGCAACAAAGTTGcc                                                                                                                                                  >  1:1002855/1‑150 (MQ=255)
      cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                                 >  1:1100060/1‑150 (MQ=255)
      cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                                 >  1:421019/1‑150 (MQ=255)
      cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                                 >  2:584985/1‑150 (MQ=255)
      cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                                 <  2:852688/150‑1 (MQ=255)
       ggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcat                                                                                                                                                >  2:1299928/1‑150 (MQ=255)
       ggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcat                                                                                                                                                <  2:673210/150‑1 (MQ=255)
         cggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                              <  2:1496266/150‑4 (MQ=255)
         cggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                              <  2:117205/150‑4 (MQ=255)
         cgcaTCAGTTACAGGCTTGCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                              <  1:161701/147‑4 (MQ=255)
          ggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcatcat                                                                                                                                             >  1:905832/1‑150 (MQ=255)
          ggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGATGGCAACTTTGTTGCcaaccg                                                                                                                                             <  1:224245/150‑5 (MQ=255)
           gATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCatcatc                                                                                                                                            <  2:1386978/150‑1 (MQ=255)
                gTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTtg                                                                                                                                       >  1:1826587/1‑150 (MQ=255)
                  tACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGCCGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCTCCGGTTGGCAACAAAGTTGCCATCATCGGTtgtg                                                                                                                                     >  1:1281327/1‑150 (MQ=255)
                   aCAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTgg                                                                                                                                    >  2:1102638/1‑150 (MQ=255)
                    cAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGc                                                                                                                                   <  1:451143/150‑1 (MQ=255)
                    cAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGc                                                                                                                                   <  1:1307968/150‑1 (MQ=255)
                      ggCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgg                                                                                                                                 >  1:572923/1‑150 (MQ=255)
                      ggCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgg                                                                                                                                 >  2:46050/1‑150 (MQ=255)
                      ggCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgg                                                                                                                                 >  2:39670/1‑150 (MQ=255)
                      ggCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgg                                                                                                                                 >  2:700512/1‑150 (MQ=255)
                      ggCTTGCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgg                                                                                                                                 <  1:1764020/150‑1 (MQ=255)
                      ggCGTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCCCGCACGCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgg                                                                                                                                 <  1:168613/150‑1 (MQ=255)
                       gCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                                <  2:38848/150‑1 (MQ=255)
                        cTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCgggt                                                                                                                               >  1:1941357/1‑149 (MQ=255)
                        cTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGa                                                                                                                               <  2:483896/150‑1 (MQ=255)
                           tCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTg                                                                                                                            <  2:1085602/150‑1 (MQ=255)
                              aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                         >  2:1187297/1‑150 (MQ=255)
                                 aaaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                      <  1:62218/150‑1 (MQ=255)
                                 aaaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                      <  2:1616444/150‑1 (MQ=255)
                                 aaaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                      <  1:483124/150‑1 (MQ=255)
                                  aaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTTGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGa                                                                                                                     >  2:1467672/1‑150 (MQ=255)
                                  aaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGa                                                                                                                     >  1:1061153/1‑150 (MQ=255)
                                  aaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGACGGTTGGCAACAAAGTTGCCATCATCGGTTTTGGCGGGATTGGTTTTGa                                                                                                                     >  1:1995422/1‑150 (MQ=255)
                                     gATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATAc                                                                                                                  >  2:823273/1‑150 (MQ=255)
                                        ccTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGc                                                                                                               >  2:989048/1‑150 (MQ=255)
                                        ccTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGc                                                                                                               >  2:694402/1‑150 (MQ=255)
                                         cTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAGTGGTTTTGATACGGCg                                                                                                              >  2:580234/1‑150 (MQ=255)
                                            gCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATg                                                                                                           <  1:1325857/150‑1 (MQ=255)
                                             ccAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGt                                                                                                          <  1:766896/150‑1 (MQ=255)
                                              cAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTAGTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGGGGCGGGCTTGGTTTTGATACGGCGATGTa                                                                                                         >  1:247172/1‑150 (MQ=255)
                                                 tGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAttt                                                                                                      <  2:1763193/150‑1 (MQ=255)
                                                   ggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTaa                                                                                                    <  2:1947513/150‑1 (MQ=255)
                                                              cgcACTCCGCCCATCGCCGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCAGCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGc                                                                                         >  1:163446/1‑150 (MQ=255)
                                                               gcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCg                                                                                        <  2:1100060/150‑1 (MQ=255)
                                                                  cTCCGCCCATCGACTGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAAt                                                                                     <  1:1863441/150‑1 (MQ=255)
                                                                  cTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAAt                                                                                     <  2:123667/150‑1 (MQ=255)
                                                                  cTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAAt                                                                                     <  1:1184835/150‑1 (MQ=255)
                                                                   tCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATc                                                                                    <  2:2014899/150‑1 (MQ=255)
                                                                    ccGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATcc                                                                                   >  1:1666159/1‑150 (MQ=255)
                                                                    ccGCCCATCCACGGGATAGAGCATACGAAGGTATTTAGTTATATCGATGTACTGCGCGCCAAAGCGCCGGGTTGCAACCAAGTTGCCATAATCGGTTTTTGCGGGATTGGTTTTGATACGGCGATTTATTTAAGTCAGCCGGGCGAAtac                                                                                   >  2:1146025/1‑148 (MQ=255)
                                                                               cGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGaa                                                                        <  1:1742840/150‑1 (MQ=255)
                                                                                 ggATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAata                                                                      <  1:1177256/150‑1 (MQ=255)
                                                                                      gatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGcc                                                                 >  1:1623484/1‑150 (MQ=255)
                                                                                      gatcATCCGAAGGTATTCAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTCGTTTTGATACGGCGATGAATTTAAGTCAGCCGGTCGAAACCACCAGACAGAATATCGcc                                                                 >  1:149626/1‑150 (MQ=255)
                                                                                         catcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                              >  2:1515389/1‑150 (MQ=255)
                                                                                         catcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                              >  2:1943109/1‑150 (MQ=255)
                                                                                         catcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                              >  2:1067359/1‑150 (MQ=255)
                                                                                         catcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                              <  1:43989/150‑1 (MQ=255)
                                                                                            ccGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTc                                                           >  2:634175/1‑150 (MQ=255)
                                                                                              gAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                         <  1:700512/150‑1 (MQ=255)
                                                                                               aaGGTATTGAGTTATCTCGATGGACTGCGCGACAAAGCGCCGGGTGGCAACAAAGCTGCCATCATCGGTTGGGGCGGGAATGGTTTTGATACGGCGAGGTATTTAAGTCAGCCGGGCGAATCCACCAGGCAGAATATCGCCGGGTTCTGt                                                        <  1:76680/150‑1 (MQ=255)
                                                                                                        aGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATgg                                               >  2:1196903/1‑150 (MQ=255)
                                                                                                          ttctctcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAACGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATgggg                                             <  2:1855359/147‑1 (MQ=255)
                                                                                                           tATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggggg                                            <  2:344269/150‑1 (MQ=255)
                                                                                                           tATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggggg                                            >  1:136314/1‑150 (MQ=255)
                                                                                                            aTCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCCCCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGa                                           >  2:80829/1‑150 (MQ=255)
                                                                                                               tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                        <  2:471355/150‑1 (MQ=255)
                                                                                                                 gATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGGTGGGGCGGGATTGGGTTTGATACGGCGATGTATTTAAGTGAGCCGGGCGAATCCAGCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGAc                                      <  2:1340282/150‑1 (MQ=255)
                                                                                                                    gTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGt                                   >  2:13214/1‑150 (MQ=255)
                                                                                                                     tACTGCGCGACAAAGCGACGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTATTGATACGGCGAAGTATTTAACTCCGCCGGGCGACTCCACCAGCCAGAATATCGCCGGGTTCGGTAATGAATGGTGGATCGACAGTa                                  >  1:170365/1‑150 (MQ=255)
                                                                                                                       gtGCGCGACATAGAGACGGGTGGCAACAAAG‑TGCCATCATCGGGTGTGGCGGGATGGGTGTTGATACGGCGTTGTATTTTAGTCAGTCGGGCGAATCCACCAGTCAGAATATCGCCGGGTTCTGTAATGTATGGGGGATCGACAGTAGcc                               <  1:269088/149‑1 (MQ=255)
                                                                                                                        tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                               >  2:1125664/1‑150 (MQ=255)
                                                                                                                          cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTa                             <  1:846392/150‑1 (MQ=255)
                                                                                                                           gcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAGTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGTGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTac                            <  1:397142/150‑1 (MQ=255)
                                                                                                                           gcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTGGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTac                            <  2:1859090/150‑1 (MQ=255)
                                                                                                                            cgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCCGCCAGAATATCGCCGGGTTCTGTATTGAATGGGGGATCGACAGTAGCCTaca                           >  2:358375/1‑150 (MQ=255)
                                                                                                                            cgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATTAATGGGGGATCGACAGTAGCCTaca                           >  2:1619386/1‑150 (MQ=255)
                                                                                                                            cgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGGTCTGTAATGAATGGGGGATCGACAGAAGCCTaca                           >  2:944420/1‑150 (MQ=255)
                                                                                                                                aaaGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAg                       >  1:148118/1‑150 (MQ=255)
                                                                                                                                  ggcgcCGGTTGGCAACAAAGTTGCCATCATCGGGTGGGGCGGGAGTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGACTCCACCAGCCAGAATATCGCCGGGGTCTGTAATGACTGGGGGATCGTCAGTAGCCGACAACAGGc                     >  1:1420931/2‑150 (MQ=255)
                                                                                                                                  aGCGCCGGTTGGCAACAAAGTTGCCATCATCGCTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCGCGAATATCGCCGGGTTCAGAAATGAATCGGGGATCGACAGTAGCCTACAACAGGc                     >  1:295280/1‑150 (MQ=255)
                                                                                                                                   gcgcCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCt                    >  1:2014907/1‑150 (MQ=255)
                                                                                                                                     cgatgatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGCTCGACAGTAGCCTACACCAGGCtgg                  >  1:990790/7‑150 (MQ=255)
                                                                                                                                       cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg                >  2:772049/1‑150 (MQ=255)
                                                                                                                                        ggTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtgg               <  2:635566/150‑1 (MQ=255)
                                                                                                                                        ggTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtgg               <  2:1061153/150‑1 (MQ=255)
                                                                                                                                          atGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGGCTACAACAGGCTGGTGGCt             >  2:280014/2‑150 (MQ=255)
                                                                                                                                             gCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTaa          >  1:536014/1‑150 (MQ=255)
                                                                                                                                             gCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTaa          <  2:1014794/150‑1 (MQ=255)
                                                                                                                                              caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTTGCTTaac         >  1:1315970/1‑149 (MQ=255)
                                                                                                                                              caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAg         >  2:1198104/1‑150 (MQ=255)
                                                                                                                                              caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAg         >  2:1201006/1‑150 (MQ=255)
                                                                                                                                                 caaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGccc      <  1:861000/150‑1 (MQ=255)
                                                                                                                                                  aaaGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCg     <  1:1970580/150‑1 (MQ=255)
                                                                                                                                                    aGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCA‑CAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAg  <  1:1040936/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAG  >  NC_000913/3233007‑3233305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑