Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,788 T→A 5.4% intergenic (‑44/+39) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,7880TA 5.4% 485.2 / 5.9 162intergenic (‑44/+39)ygjV/uxaAImp‑YgjV family inner membrane protein/altronate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (80/71);  new base A (5/5);  total (85/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

CCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTCCAG  >  NC_000913/3241639‑3241937
                                                                                                                                                     |                                                                                                                                                     
ccGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACAt                                                                                                                                                       >  2:704034/1‑150 (MQ=255)
ccGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACAt                                                                                                                                                       >  2:596408/1‑150 (MQ=255)
   aaaGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                                    <  1:654737/150‑1 (MQ=255)
   aaaGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCATGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                                    <  2:1092407/150‑1 (MQ=255)
    aaGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATg                                                                                                                                                   >  2:1974986/1‑150 (MQ=255)
      gctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGaa                                                                                                                                                 <  2:547085/150‑1 (MQ=255)
      gctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGaa                                                                                                                                                 <  2:1713137/150‑1 (MQ=255)
      gctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCGTCACATTATGaa                                                                                                                                                 >  2:1049691/1‑150 (MQ=255)
        tgctTTTTGAAGCGACGTTCGTCACGATTGAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACg                                                                                                                                              >  1:234535/1‑150 (MQ=255)
         gctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACg                                                                                                                                              >  2:1930945/1‑150 (MQ=255)
         gctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTAACATTATGAAACg                                                                                                                                              >  1:264812/1‑150 (MQ=255)
         gctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGCCGCTTCACATTATGAAACg                                                                                                                                              >  1:1533127/1‑150 (MQ=255)
            tttaGAAGCGACGTTCGTCACGAGTGAAACATGTTGTGATACCAATCAGAAAGGCGAGGACACCCACGCCCTGGGCCAGCCAAAACGCGGTCATGATAACACCTTAGCAGGTAGGGAACAGCAAACGGCGCTTCACATTATGAAACGCCg                                                                                                                                           <  1:120787/150‑1 (MQ=255)
             tttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGTTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGt                                                                                                                                          >  1:516643/1‑150 (MQ=255)
                gAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGtttt                                                                                                                                       >  2:1688080/1‑150 (MQ=255)
                 aaGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGttttt                                                                                                                                      >  2:996926/1‑150 (MQ=255)
                  aGCGACGTTCGTCACGATTGAAAACTGTCGTGATACCAATCAGAACGGCGATGACCCCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTCGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGtttgct                                                                                                                                     <  2:1002603/150‑4 (MQ=255)
                  aGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGGCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGtttttt                                                                                                                                     <  2:436584/150‑1 (MQ=255)
                     gACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGtttgctttt                                                                                                                                  <  2:6665/150‑7 (MQ=39)
                     gACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTttatt                                                                                                                                  <  2:721942/150‑1 (MQ=255)
                                cGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAAGAGGTCATGATAAATCATTAGCAGGTATGGAAAAGCAAACGGCTCTTCACATTATAAAAATCCCTTTTATATTAACAACACAtt                                                                                                                       >  1:326022/1‑150 (MQ=255)
                                     gAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGAGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAc                                                                                                                  >  1:816332/1‑150 (MQ=255)
                                     gAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATCATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAc                                                                                                                  >  2:778032/1‑150 (MQ=255)
                                      aaaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACt                                                                                                                 <  2:1137222/150‑1 (MQ=255)
                                       aaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAATAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                                <  1:227523/150‑1 (MQ=255)
                                       aaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                                <  2:1788711/150‑1 (MQ=255)
                                       aaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTGTTTATTAACAACTCATTTCGACtt                                                                                                                >  2:938457/1‑150 (MQ=255)
                                        aaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACttt                                                                                                               >  1:1737800/1‑150 (MQ=255)
                                        aaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACttt                                                                                                               <  2:1635485/150‑1 (MQ=255)
                                            gttgtGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAg                                                                                                           >  2:799787/1‑150 (MQ=255)
                                            gttgtGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAg                                                                                                           <  2:904670/150‑1 (MQ=255)
                                              tgtgATCCCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATCCGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCg                                                                                                         <  2:515080/150‑1 (MQ=255)
                                                tgATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGtt                                                                                                       >  2:235513/1‑150 (MQ=255)
                                                 gATACCAATCAGAAATGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAACGCAAACGGCGCTTCACAGTATGAACCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTa                                                                                                      <  1:322255/150‑1 (MQ=255)
                                                 gATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTa                                                                                                      <  1:1940857/150‑1 (MQ=255)
                                                 gATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAAACCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTAATAGCGTTa                                                                                                      <  2:965366/150‑1 (MQ=255)
                                                  aTACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAc                                                                                                     >  2:877074/1‑150 (MQ=255)
                                                      cAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgccg                                                                                                 >  1:599711/1‑150 (MQ=255)
                                                       aaTCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgccgc                                                                                                >  1:1136453/1‑150 (MQ=255)
                                                         tCAGAAAGGCGTTGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCtt                                                                                              <  1:1063011/150‑1 (MQ=255)
                                                          cAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCttt                                                                                             >  2:1555010/1‑150 (MQ=255)
                                                            gAAAGGCGATGACACCCACGCCCTTGGCCAGCCAATACTCGGTAATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTg                                                                                           <  1:855869/150‑1 (MQ=255)
                                                             aaaGGCGATGCCACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGa                                                                                          <  2:1201629/150‑1 (MQ=255)
                                                             aaaGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGa                                                                                          <  2:2001579/150‑1 (MQ=255)
                                                               aGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAg                                                                                        <  1:1546354/150‑1 (MQ=255)
                                                                  cGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGTCTCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATc                                                                                     <  2:333771/150‑1 (MQ=255)
                                                                     tGACACCCACGCCCTGGTCCAGCCAATACGCGGTCATTATAAATCCTTAGCAGGTATTGAAAACCAAACGGCGCTTAACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGcc                                                                                  <  1:1648101/150‑1 (MQ=255)
                                                                     tGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGcc                                                                                  <  2:305348/150‑1 (MQ=255)
                                                                        cacCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGt                                                                               >  1:1745845/1‑150 (MQ=255)
                                                                         acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGtt                                                                              >  2:900098/1‑150 (MQ=255)
                                                                           caaCGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTcg                                                                            <  1:1100631/148‑1 (MQ=255)
                                                                                     ggCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGt                                                                  <  2:1620291/150‑1 (MQ=255)
                                                                                     ggCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAAGCCGCTTTTGAAGATCGCCAGTTAGCTGAAGTCGt                                                                  >  1:1823902/1‑150 (MQ=255)
                                                                                        cagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAATATCTCCAGTTCTCGGAATTCGTTAc                                                               >  2:1791799/1‑150 (MQ=255)
                                                                                        cagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTAc                                                               >  2:1495776/1‑150 (MQ=255)
                                                                                          gccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGc                                                             <  1:451215/150‑1 (MQ=255)
                                                                                          gccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGc                                                             >  1:1176456/1‑150 (MQ=255)
                                                                                            caATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTc                                                           >  2:1895614/1‑150 (MQ=255)
                                                                                            caATACGCGGTCATGATAAATCCTTAGCAGGGATGGAAAAGCAAACGGCGCTTCACAATATGAAACGCCGTTTTTTATTAAAAACTCATTTCGACTTTATAGCGTTACGAGGCATTTGATGATCGCCAGATCGCGGAAGTCGTTAAGCTc                                                           >  2:189333/1‑150 (MQ=255)
                                                                                             aaTACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGGAAACGGCGCTTAACATTATGAAACGCCGTTTTTTATTAACATCTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCg                                                          <  2:434289/150‑1 (MQ=255)
                                                                                             aaTACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTATATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCg                                                          <  1:171274/150‑1 (MQ=255)
                                                                                                    ggTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAgg                                                   <  1:1129073/150‑1 (MQ=255)
                                                                                                      tCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGtt                                                 >  1:915335/1‑150 (MQ=255)
                                                                                                      gcATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACAATTTGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGtt                                                 <  2:195794/149‑1 (MQ=255)
                                                                                                        aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTg                                               >  1:1826192/1‑150 (MQ=255)
                                                                                                        aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTg                                               <  1:407367/150‑1 (MQ=255)
                                                                                                        aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTg                                               <  2:1112163/150‑1 (MQ=255)
                                                                                                        aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTg                                               <  1:1166098/150‑1 (MQ=255)
                                                                                                          gATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCt                                             >  1:38954/1‑150 (MQ=255)
                                                                                                              aaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAcc                                         <  1:617964/150‑1 (MQ=255)
                                                                                                                tCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGt                                       >  2:1750171/1‑150 (MQ=255)
                                                                                                                tCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGt                                       <  1:1658402/150‑1 (MQ=255)
                                                                                                                  cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                                     <  2:1181052/150‑1 (MQ=255)
                                                                                                                    tAGCATGTATGGAAAAGCAAACGGCGCTTCACTTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGc                                   <  1:1015752/150‑1 (MQ=255)
                                                                                                                    tAGCAGGTATGGTAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAAGAACTGATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGc                                   <  2:326022/150‑1 (MQ=255)
                                                                                                                      gCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCaa                                 >  2:818614/1‑150 (MQ=255)
                                                                                                                       cAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGGTACAGGTTTGCTTACCGTTGGccac                                >  2:1705336/1‑147 (MQ=255)
                                                                                                                        aGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAAc                               <  1:704034/150‑1 (MQ=255)
                                                                                                                          gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTc                             <  2:1580935/150‑1 (MQ=255)
                                                                                                                           tATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCa                            <  1:1358135/150‑1 (MQ=255)
                                                                                                                              ggAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTGCAGGTTTGCTTACCGTTGGCAAACTCAACg                         >  1:1016796/1‑150 (MQ=255)
                                                                                                                              ggAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACg                         >  2:1140639/1‑150 (MQ=255)
                                                                                                                                 ataaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                      >  1:1989206/6‑150 (MQ=39)
                                                                                                                                 ataaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACACATTTCGACTTTATAGCTTTTCGCCGCTTTTGAAGATAGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTTCTTACCGTTGGCAAACTCAACGATg                      >  1:1006775/6‑150 (MQ=37)
                                                                                                                                 aaaGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                      >  2:1861185/1‑150 (MQ=255)
                                                                                                                                  aaGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATgg                     <  2:624908/150‑1 (MQ=255)
                                                                                                                                  aaGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCAGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTCCGTTTGGAAACAGAACggttgg                    >  1:915667/1‑145 (MQ=255)
                                                                                                                                  aaGCAAACGGCGCTTCAATTTATTAACCGCCGTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgt                    <  2:1529613/150‑1 (MQ=255)
                                                                                                                                   aGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgt                    >  1:353759/1‑150 (MQ=255)
                                                                                                                                     cAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgtgt                  >  2:1510668/1‑150 (MQ=255)
                                                                                                                                     cAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgtgt                  >  1:883669/1‑150 (MQ=255)
                                                                                                                                      aaaCGGCGCTTCACATTATGAAACGCCGTTTATTATTAACAACTCATTTCGACTTTATAGCGTTACGCAGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGAGTc                 >  1:1972655/1‑150 (MQ=255)
                                                                                                                                       aaCGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCg                <  1:1420804/150‑1 (MQ=255)
                                                                                                                                           gcgcTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATaa            <  2:1053124/150‑1 (MQ=255)
                                                                                                                                             gcTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAt          >  1:242433/1‑150 (MQ=255)
                                                                                                                                              cTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAtt         <  2:234535/150‑1 (MQ=255)
                                                                                                                                                  acaTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc     <  1:1266477/150‑1 (MQ=255)
                                                                                                                                                  acaTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc     >  1:1577634/1‑150 (MQ=255)
                                                                                                                                                   caTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTcc    >  2:1375014/1‑150 (MQ=255)
                                                                                                                                                   caTTATGAAACGCCGTTATTTATTAACAAATCATTTCGACTTTAAAGCGATACGCCGCTTTTGAAGATCTCCAGTTCGCGGAAGTCGTTACGCTCCTTACAGGTTTGCTTACCCTTGGAAAACTCAACGATGGTCTCGATAAAttcttca    >  1:1049998/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTCCAG  >  NC_000913/3241639‑3241937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: