Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,956 T→C 14.7% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9560TC14.7% 105.1 / 15.0 64intergenic (+354/+260)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (7/41);  new base C (2/6);  total (16/48)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-02

CGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAC  >  NC_000913/3269807‑3270101
                                                                                                                                                     |                                                                                                                                                 
cgccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                                                                                                                   <  1:539218/150‑1 (MQ=255)
   cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAAACTTTATGCCCCTCCAAAAGaaaa                                                                                                                                                >  2:1677124/1‑150 (MQ=255)
    gcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaa                                                                                                                                               <  1:1698810/150‑1 (MQ=255)
      aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAc                                                                                                                                             <  2:546420/150‑1 (MQ=255)
      aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAAc                                                                                                                                             >  1:1440283/1‑150 (MQ=255)
       tCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAcc                                                                                                                                            <  1:1698765/150‑1 (MQ=255)
           gCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgcc                                                                                                                                        <  2:721575/150‑1 (MQ=255)
            cATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgccg                                                                                                                                       <  1:674443/150‑1 (MQ=255)
             aTGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGAAAAAAcaccgaca                                                                                                                                      >  1:1453349/1‑143 (MQ=255)
              tGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTAATATTTCTGCCCCACCAAAAGTAAAAACACGCCGaa                                                                                                                                     >  1:770227/1‑150 (MQ=21)
                    aaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTAATCTTTCTGGCCCTCCAAAAGAAAAAAACCGCCGAAGCgggg                                                                                                                               >  2:1518389/1‑149 (MQ=18)
                     aGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTCATCTTTCTGCCCCGCCAAAACTAAAAACCCGCCGAAGCGGGtt                                                                                                                              >  1:1578763/1‑150 (MQ=255)
                       cgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtttt                                                                                                                            <  1:1086132/150‑1 (MQ=255)
                         cACTCAAAAATTTCAAACCCGCCGTATCGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                          <  2:195643/150‑1 (MQ=11)
                          aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                         <  1:841585/150‑1 (MQ=255)
                           cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTATGCCCCTCCAAAATTAAAAACCCGCATAAGCGGGttttttct                                                                                                                        >  2:164678/1‑147 (MQ=18)
                           cTCAACAATCTGAAACCCCCCGGAACGGGTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                        <  1:117158/150‑1 (MQ=255)
                           atCAACAATCTTAAACCCGCCGGAACGTTTTATTACGTACATCATGTAAAACTGACCTATAAGCAGCTTTCTTTTTCGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                        <  1:722222/149‑1 (MQ=17)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                         >  2:1231705/1‑147 (MQ=255)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                         <  1:1231705/147‑1 (MQ=255)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                                      <  2:1509540/150‑1 (MQ=255)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                                      <  2:323630/150‑1 (MQ=255)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                                      <  1:769534/150‑1 (MQ=255)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTAAAACAGGTAAAACTGACCAAAAAGCATCATACTATTGTATATAGTGTCTTGTAAATTCATTAATAATTCTTCCCCGCCAAAAGAAAAAAACCAACAAAGCGGGTTTTTACgga                                                                                                                      >  2:41183/1‑148 (MQ=255)
                              aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                                                     <  1:1716393/150‑1 (MQ=255)
                               acaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                    <  2:459501/150‑1 (MQ=255)
                               acaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                    <  1:817506/150‑1 (MQ=255)
                                caaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                   <  2:572838/150‑1 (MQ=255)
                                 aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                  <  1:363799/150‑1 (MQ=255)
                                   acTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg                                                                                                                <  1:1878930/149‑1 (MQ=255)
                                     aaaaaCCCGCCGTAGAGGTTTATTACGTACATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                                                                                              <  2:131211/148‑1 (MQ=11)
                                        aaCCCGCCGTAACTGTTTATTACGTACATCAGGTAAAACTGACCGATAAGACGCTTTCTTTTGGTTATAGTGTCGTGTACAGTCATTCATCTTTCTGACCCTCCTTAAGTAAAAACCCGCCGATGCGGGTTTTTAATTAAAAAAGGTGaa                                                                                                           <  2:1521479/150‑1 (MQ=255)
                                           ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGCATCATATTTCTTGCCCTACAAAAGAAAAAAACCGCCGAAGCGGGGTTTTAAGTAAAAAAGGtttaacg                                                                                                        >  1:1470527/1‑144 (MQ=17)
                                               cgtatcGGTTTATTACGTACATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                    <  2:529808/145‑1 (MQ=11)
                                               cgccaCGGTTTATTACGTACATCATGTTAAACTGACCGATTAGCCGCTTTCTTTTGGGTATAGGGGCGTGGACAGGCATTCATCTTTCAGCACCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                    <  1:41183/146‑1 (MQ=11)
                                               cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATTAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                    <  2:1892218/150‑1 (MQ=255)
                                               cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                    <  2:1144378/150‑1 (MQ=255)
                                               cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                    <  1:1458031/150‑1 (MQ=255)
                                               cgGAACGGTTTATTACGTACATCAGGTAAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                    <  1:396109/150‑1 (MQ=255)
                                                ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                   <  2:1974502/150‑1 (MQ=255)
                                                     ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGTGTTTAGTGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                              <  1:1598797/150‑1 (MQ=255)
                                                     ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                          <  2:1968319/122‑1 (MQ=255)
                                                     ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                          >  1:1968319/1‑122 (MQ=255)
                                                          attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAACAAAAACCCGCCGAAGCGGGtt                                                                                                                              >  1:732572/1‑113 (MQ=255)
                                                          attaCGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAACAAAAACCCGCCGAAGCGGGtt                                                                                                                              <  2:732572/113‑1 (MQ=2)
                                                               gTACATCAGGAAAAACTGAGCGATAAGCCGCTTGCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttctt                                                                                    <  2:210587/150‑1 (MQ=255)
                                                                   atcatttAAAAATGACCGTTAATTAGCTTTCTTTTTGTTATAGTGTCGTGGACAGTCTTTATTCTTTATGTCACTCCAAAAGTATAAACCCGCCGTATCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                <  2:1101122/144‑1 (MQ=11)
                                                                   aTCAGGTAAAACTGACCGATATTCCGCTTTCTTTTGTTTTATGTGTCGTGGACAGTGATTTATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                <  2:6172/150‑1 (MQ=11)
                                                                    acAGGTAACTCTGAGCGATAAGCCGTTTTCTTTTGTGTTTTGTGTCGTGGATAGTGTTTCTTCTTTCTGGCACTCCAAAATTTCAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                               <  2:1731539/149‑1 (MQ=11)
                                                                    acAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                               <  2:473287/149‑1 (MQ=25)
                                                                       ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                       >  2:342942/1‑107 (MQ=17)
                                                                         tAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGtttt                                                                                                                            >  2:54057/1‑100 (MQ=17)
                                                                                gacatatatGCCGCTTTGTTTTTTGTTTTGTGTCGTTGACTGTGTTTAATATTTATGCCACTCCAAAATTAAAAACCCGCCGAATCTGTTTTTTACGTAAAACAGTTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                   <  1:1651765/145‑1 (MQ=2)
                                                                                         ttcGCTTTCTTTTGGGTTTTGTGTCGTGGAAAGTCTTTCATCTTTCTTCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcat                                                          <  1:1903755/148‑1 (MQ=11)
                                                                                                     tgtttttaGTGTCGTCGACAGTGTTTTATCTTTATGGCACTCAAAAATTATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGc                                              <  2:683077/144‑1 (MQ=11)
                                                                                                           ttgtgaCGCGGACAGCCATTCTTCTTTCGGCCCCTCCAAAAGTACAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                        <  2:1583037/148‑1 (MQ=11)
                                                                                                             gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAg                                                              >  1:1447911/1‑126 (MQ=17)
                                                                                                                         gTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                             >  1:300956/1‑67 (MQ=255)
                                                                                                                            atttttCTTTCTGGCACTCAAAAATTAAAAACCCGCCGAATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                       <  1:1677124/145‑1 (MQ=11)
                                                                                                                              tgatCTTTCTGCTCCTCATATATTTGAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                     <  1:909887/148‑1 (MQ=11)
                                                                                                                              tcttCTATCTGCCACTCCAAAATTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                     <  1:1735794/147‑1 (MQ=11)
                                                                                                                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCggg                                                                                         >  2:1401228/1‑78 (MQ=255)
                                                                                                                                   tatCTGCCCCTCAAAAATTACAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                <  2:818203/148‑1 (MQ=11)
                                                                                                                                          cactacAAAAGTTTAAACCCGCCGTACCGGGTTTTTACGTAAAACATGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttg         <  2:346746/145‑2 (MQ=11)
                                                                                                                                            cTCAAAAATTACAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg       <  2:1593983/150‑1 (MQ=11)
                                                                                                                                                 aaaGTTAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc  <  2:1486423/150‑1 (MQ=38)
                                                                                                                                                     |                                                                                                                                                 
CGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAC  >  NC_000913/3269807‑3270101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: