Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,288,032 C→A 9.2% intergenic (+22/‑58) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,288,0320CA 9.2% 354.5 / 21.3 135intergenic (+22/‑58)yraH/yraIputative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (70/52);  new base A (8/5);  total (78/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 138 total aligned reads displayed.

TCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGC  >  NC_000913/3287883‑3288177
                                                                                                                                                     |                                                                                                                                                 
tCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGACGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGAcc                                                                                                                                                   <  1:1102034/150‑1 (MQ=255)
 cccGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAACTCACCCCAGGGGAAGCAGCCGCTATCGTTAACGTAACGCTGGATTACCGGTACTACGTTAGGGCGTTATCTGAccg                                                                                                                                                  >  1:988377/1‑149 (MQ=255)
 cccGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCt                                                                                                                                                  <  2:1303678/150‑1 (MQ=255)
   cggcggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGTAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTTACCTGt                                                                                                                                                >  1:461853/1‑150 (MQ=255)
    ggcggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAATTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTc                                                                                                                                               <  2:1284433/150‑1 (MQ=255)
       ggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGa                                                                                                                                            >  1:217866/1‑150 (MQ=255)
          aTCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGCCCTGTCAGATaa                                                                                                                                         >  2:879045/1‑150 (MQ=255)
            ccAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTTTCGTTAAAGTAAAGATGGATTACCGTTAATACTTTAGGGCGTTATCTGACCTGTCAGATAACg                                                                                                                                       >  2:1226158/1‑150 (MQ=255)
            ccAATGGTATTGTCAATGTTGACCTCATCGCTGACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACg                                                                                                                                       <  1:839198/150‑1 (MQ=255)
            ccAAAGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACg                                                                                                                                       <  2:454626/150‑1 (MQ=255)
             cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                      <  1:286570/150‑1 (MQ=255)
             cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                      <  1:1215162/150‑1 (MQ=255)
             cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTAACAACCGTAGTTCCATCAGAAATACCCCCAGGGGAAGAAGACGCTATCGGTAACTTAACGCTGGAGTACAGATAAGACGTTAGGGCGTTATCGGACCTGTCAGATATCGc                                                                                                                                      >  2:1230226/1‑150 (MQ=255)
              aaTGGTATTGTCAATGTTGTCCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGATGCAGACGCTATCGTTAACGTAATGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGcc                                                                                                                                     <  2:80018/150‑1 (MQ=255)
                      tGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcct                                                                                                                             >  1:1161761/1‑150 (MQ=255)
                       gTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcctt                                                                                                                            >  2:172691/1‑150 (MQ=255)
                       gTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcctt                                                                                                                            <  2:795223/150‑1 (MQ=255)
                         cAATGTTGACCTCATCGCTTACTACGTATCAACGGTAGTTCCATCAGAAATCTCCCGAGGCGAAGCAGACGCTATCGTTAACGTATCGCTGGATTACCGTTAATAGGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                                                                          <  1:1629994/150‑1 (MQ=255)
                         cAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGTAAGCAGACGCTATCGTTAACGAAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                                                                          <  1:930267/150‑1 (MQ=255)
                            tGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGGTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTGCcgct                                                                                                                       >  1:1469191/1‑150 (MQ=255)
                             gTTGCCCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGACAGATAACGCCCTTTTACTTCCTCtt                                                                                                                      >  1:954439/1‑150 (MQ=255)
                             gTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTACCGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttccgggt                                                                                                                      >  2:95128/1‑146 (MQ=255)
                             gTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCtt                                                                                                                      >  2:248589/1‑150 (MQ=255)
                              ttGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCATAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCttt                                                                                                                     <  1:489504/150‑1 (MQ=255)
                                     cATCGCTTACTACGTATCAATCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTg                                                                                                              <  2:402733/150‑1 (MQ=255)
                                     cATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGACGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTg                                                                                                              <  2:2013184/150‑1 (MQ=255)
                                      gtCGCTTACTACGTATCAACCGTAGTTCCCTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTATTACGTTAGGGCGTTATGTGACCGGGCAGCTAACGCCCGTTTACTTACTCTTTCTCGgtgt                                                                                                             >  1:1463463/2‑150 (MQ=255)
                                      aTCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTATCGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGCTAACGCCCTTTTCCTTCCTCTTTGTCGTTGt                                                                                                             >  1:1463460/1‑150 (MQ=255)
                                      aTCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGt                                                                                                             >  1:1607283/1‑150 (MQ=255)
                                       tCGCTTACTACGTATCAACCTTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                                                                            <  1:1774849/150‑1 (MQ=255)
                                         gCTTACTACGTATCACCAGTAGTTCCATCATAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCATCCTCTTTCTCGTTGTATc                                                                                                          <  1:1000079/150‑1 (MQ=255)
                                          cTTACTACGTATCAACCGTAGTTCTATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCa                                                                                                         <  1:818920/150‑1 (MQ=255)
                                             actacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCCTTGTATCAGGt                                                                                                      >  1:1430991/1‑150 (MQ=255)
                                                 cGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTGCTCGTTGTATCAGGTTGaa                                                                                                  >  2:832924/1‑150 (MQ=255)
                                                   tATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGACGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTAATCTCACCTCTCAGATAACGCCCTTTTACTTCCTCTTTCTCGTTGTATCACGTTGaaaa                                                                                                >  2:104429/1‑150 (MQ=255)
                                                   tATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaa                                                                                                >  2:196865/1‑150 (MQ=255)
                                                    aTCAACCGTAGTTCCATCAGAAATCCCCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaaa                                                                                               <  1:370889/150‑1 (MQ=255)
                                                      cAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTTTCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATa                                                                                             >  1:1936118/1‑150 (MQ=255)
                                                       aaCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATaa                                                                                            <  2:145381/150‑1 (MQ=255)
                                                       aaCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATaa                                                                                            >  2:650900/1‑150 (MQ=255)
                                                       aaCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATaa                                                                                            >  2:225006/1‑150 (MQ=255)
                                                       aaCCGTAGTTCCATCAGAAATCACCCCAAGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTAAGGGCGTTATCTGACATGTCAGAAAACCCCCTTTTACTTCCTATTTCTCGTTGTATAAGGTTGAAAAATaa                                                                                            >  1:417536/1‑150 (MQ=255)
                                                        aCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATaaa                                                                                           >  2:1683366/1‑149 (MQ=255)
                                                         ccGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACt                                                                                          <  2:1399077/150‑1 (MQ=255)
                                                          cGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTa                                                                                         >  2:1569035/1‑150 (MQ=255)
                                                                tCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaa                                                                                   >  2:1728527/1‑150 (MQ=255)
                                                                   aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTTATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                >  2:302536/1‑150 (MQ=255)
                                                                   aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                <  2:649953/150‑1 (MQ=255)
                                                                   aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                >  2:146697/1‑150 (MQ=255)
                                                                      aGAAATCACCCCAGGGGAAGCAGACGCTAACGTTAAAGTAACGCTGGATTACCGTTAATACGTAAGGGCGTTATCTGACCTGTCAGATTAAGCCCTTTACCTTCATCTTTCTCATTGTATCAGGTTGAAAATTAACTAAGTCAAAACGaa                                                                             >  2:746281/1‑150 (MQ=255)
                                                                        aaaTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTACCGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTACTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAATACGAACa                                                                           >  2:1023485/1‑150 (MQ=255)
                                                                          aTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACAtt                                                                         <  2:1565915/150‑1 (MQ=255)
                                                                            cACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAATTAACTATGTCAAAACGAACATTcc                                                                       >  2:1328671/1‑149 (MQ=255)
                                                                                 cAGGGGAAGCAGACGCTATCGTTAACGTAACGCCGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTg                                                                  <  2:1913843/150‑1 (MQ=255)
                                                                                   ggggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                >  2:1612140/1‑150 (MQ=255)
                                                                                   ggggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTCGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                <  2:698767/150‑1 (MQ=255)
                                                                                   ggggAAGCAGACGCTATCGATAACGTAACGCTGGATTACCCTTAATACGTTAGTGCGTTATATGACCTGTCAGATAACGCCCTTTTCCTACCTATTTCTCGTTGGATAAAGTTGAAAAATAACTATGTCAAAACGAACAATCTCGGTGat                                                                >  2:1100677/1‑150 (MQ=255)
                                                                                        aGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTaa                                                           >  1:680722/1‑150 (MQ=255)
                                                                                         gCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTTACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACTAACATTCGCGGTGATATTAAc                                                          <  2:557860/150‑1 (MQ=255)
                                                                                            gACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCtt                                                       >  1:474787/1‑150 (MQ=255)
                                                                                              agCTATAGTTAACGTAACTCTGGATTACCGTTAATAAGTTAGGGAGTTATCTGACCTTTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATAAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgt                                                     <  1:177272/149‑1 (MQ=255)
                                                                                                cTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg                                                   <  2:910288/150‑1 (MQ=255)
                                                                                                    cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTGCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATTACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTgtgtt                                               >  1:396421/1‑149 (MQ=255)
                                                                                                    cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                               <  2:756151/150‑1 (MQ=255)
                                                                                                    cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAATATGACAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                               >  1:1863433/1‑150 (MQ=255)
                                                                                                            taacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGt                                       <  2:1192029/150‑1 (MQ=255)
                                                                                                              acgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTGTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAt                                     >  2:166560/1‑150 (MQ=255)
                                                                                                               cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAATATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                    >  2:1571211/1‑150 (MQ=255)
                                                                                                               cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                    <  2:1796068/150‑1 (MQ=255)
                                                                                                               cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                    >  2:1992694/1‑150 (MQ=255)
                                                                                                                 gtGGTTTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTgg                                  <  1:441207/149‑1 (MQ=255)
                                                                                                                  gggATTACCGTTAATACGTTAGTGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGc                                 <  1:1755115/149‑1 (MQ=255)
                                                                                                                    gATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCa                               <  1:1170774/150‑1 (MQ=255)
                                                                                                                        aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                           >  2:709785/1‑150 (MQ=255)
                                                                                                                        aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                           >  2:605646/1‑150 (MQ=255)
                                                                                                                        aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                           <  1:861067/150‑1 (MQ=255)
                                                                                                                         ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGCTAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGCACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcgc                          >  1:180885/1‑150 (MQ=255)
                                                                                                                         ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTGCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTTATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcgg                          >  2:248867/1‑149 (MQ=255)
                                                                                                                         ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTAAAAAAGAACATTAGCGGGGATATTAACATTGTTGTGTAGCTTCTGTATTGGCCAGgcgc                          >  1:701043/1‑150 (MQ=255)
                                                                                                                         ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTACTCTTTCTCGTTGTATCAGGGTGAAAAAAAACTATCTCAAAACCAACATTCGCGGTGATATTAACCTTGTTGTGTAGATTCTGTATTGGCCAGgcgc                          >  2:1272680/1‑150 (MQ=255)
                                                                                                                             tAATACGTTAGGGCGTTATCTGACCTGTAAGATAACGAACTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAACACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGc                      <  2:954439/150‑1 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTATCCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                 >  1:1838200/1‑150 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                 <  2:1848476/150‑1 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                 >  1:1434050/1‑150 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                 <  1:441147/150‑1 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGGGATATTAACCTTGTTGTGTAGCTTCTGTATTGGTCAGGCGCTGGCaggag                 >  1:464143/1‑150 (MQ=255)
                                                                                                                                   gtaacggCGTTATCTGTCCTGACAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGATAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTTGCCAGGCGCTTGCaggagg                <  2:1258301/145‑1 (MQ=255)
                                                                                                                                   gTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggagg                >  1:1988764/1‑150 (MQ=255)
                                                                                                                                     tAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGaa              <  1:1557701/150‑1 (MQ=255)
                                                                                                                                        ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg           >  2:1665981/1‑150 (MQ=255)
                                                                                                                                        ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg           >  1:632144/1‑150 (MQ=255)
                                                                                                                                         gCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt          >  2:1702832/1‑150 (MQ=255)
                                                                                                                                         gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt          >  2:45395/1‑150 (MQ=255)
                                                                                                                                           gTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt        <  2:1607283/150‑1 (MQ=255)
                                                                                                                                             tATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAACTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTa      >  1:1964127/1‑150 (MQ=255)
                                                                                                                                              ttCTGACATGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAATATAACTATGTCAATCCGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTAc     <  1:104429/149‑1 (MQ=255)
                                                                                                                                               tCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa    >  1:1879923/1‑150 (MQ=255)
                                                                                                                                               tCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa    >  2:1412316/1‑150 (MQ=255)
                                                                                                                                                 tGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGc  >  1:1519579/1‑150 (MQ=255)
                                                                                                                                                 tGACCTGTCAGATAACGCCCTTTTCCTTCCTATTTCTCGTTGTATCATGTTGAAAAATAACTATCTCAAAACGAACATTCGCGGTTATATTAAACTTGTTGAGTAGCTAATTTATATGCAATGCGCTAGCAGGAGGAATCTTTTTACTGc  >  1:729858/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGC  >  NC_000913/3287883‑3288177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: