Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,288,033 T→G 8.8% intergenic (+23/‑57) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,288,0330TG 8.8% 349.5 / 20.3 134intergenic (+23/‑57)yraH/yraIputative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (70/52);  new base G (8/4);  total (78/56)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

Only 100 of 138 total aligned reads displayed.

CCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACG  >  NC_000913/3287884‑3288182
                                                                                                                                                     |                                                                                                                                                     
cccGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCt                                                                                                                                                       <  2:1303678/150‑1 (MQ=255)
  cggcggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGTAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTTACCTGt                                                                                                                                                     >  1:461853/1‑150 (MQ=255)
    gcggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCa                                                                                                                                                   <  2:616839/150‑1 (MQ=255)
      ggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGa                                                                                                                                                 >  1:217866/1‑150 (MQ=255)
           ccAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTTTCGTTAAAGTAAAGATGGATTACCGTTAATACTTTAGGGCGTTATCTGACCTGTCAGATAACg                                                                                                                                            >  2:1226158/1‑150 (MQ=255)
           ccAATGGTATTGTCAATGTTGACCTCATCGCTGACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACg                                                                                                                                            <  1:839198/150‑1 (MQ=255)
           ccAAAGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACg                                                                                                                                            <  2:454626/150‑1 (MQ=255)
            cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                           <  1:1215162/150‑1 (MQ=255)
            cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                           <  1:286570/150‑1 (MQ=255)
                      gTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcctt                                                                                                                                 <  2:795223/150‑1 (MQ=255)
                        cAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGTAAGCAGACGCTATCGTTAACGAAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                                                                               <  1:930267/150‑1 (MQ=255)
                          aTGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGCATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctc                                                                                                                             <  2:1271843/150‑1 (MQ=255)
                           tGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGGTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTGCcgct                                                                                                                            >  1:1469191/1‑150 (MQ=255)
                            gTTGCCCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGACAGATAACGCCCTTTTACTTCCTCtt                                                                                                                           >  1:954439/1‑150 (MQ=255)
                            gTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTACCGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttccgggt                                                                                                                           >  2:95128/1‑146 (MQ=255)
                            gTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCtt                                                                                                                           >  2:248589/1‑150 (MQ=255)
                             ttGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCATAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCttt                                                                                                                          <  1:489504/150‑1 (MQ=255)
                                    cATCGCTTACTACGTATCAATCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTg                                                                                                                   <  2:402733/150‑1 (MQ=255)
                                    cATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGACGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTg                                                                                                                   <  2:2013184/150‑1 (MQ=255)
                                    cATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGCCGGTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGCGAGTTATCTGACCTGTCAGATAACGCCCTTTACCTTCCGCTTTCTCGTTg                                                                                                                   >  2:234455/1‑150 (MQ=255)
                                     gtCGCTTACTACGTATCAACCGTAGTTCCCTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTATTACGTTAGGGCGTTATGTGACCGGGCAGCTAACGCCCGTTTACTTACTCTTTCTCGgtgt                                                                                                                  >  1:1463463/2‑150 (MQ=255)
                                     aTCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGt                                                                                                                  >  1:1607283/1‑150 (MQ=255)
                                      tCGCTTACTACGTATCAACCTTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                                                                                 <  1:1774849/150‑1 (MQ=255)
                                        gCTTACTACGTATCACCAGTAGTTCCATCATAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCATCCTCTTTCTCGTTGTATc                                                                                                               <  1:1000079/150‑1 (MQ=255)
                                         cTTACTACGTATCAACCGTAGTTCTATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCa                                                                                                              <  1:818920/150‑1 (MQ=255)
                                         cTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCa                                                                                                              <  2:1049316/150‑1 (MQ=255)
                                           tactacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTCGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAgg                                                                                                            <  2:1222111/150‑1 (MQ=255)
                                             ctacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGtt                                                                                                          >  2:941572/1‑150 (MQ=255)
                                                cGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTGCTCGTTGTATCAGGTTGaa                                                                                                       >  2:832924/1‑150 (MQ=255)
                                                 gTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaa                                                                                                      <  1:1617402/150‑1 (MQ=255)
                                                  tATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGACGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTAATCTCACCTCTCAGATAACGCCCTTTTACTTCCTCTTTCTCGTTGTATCACGTTGaaaa                                                                                                     >  2:104429/1‑150 (MQ=255)
                                                  tATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaa                                                                                                     >  2:1412011/1‑150 (MQ=255)
                                                   aTCAACCGTAGTTCCATCAGAAATCCCCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaaa                                                                                                    <  1:370889/150‑1 (MQ=255)
                                                     cAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTTTCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATa                                                                                                  >  1:1936118/1‑150 (MQ=255)
                                                      aaCCGTAGTTCCATCAGAAATCACCCCAAGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTAAGGGCGTTATCTGACATGTCAGAAAACCCCCTTTTACTTCCTATTTCTCGTTGTATAAGGTTGAAAAATaa                                                                                                 >  1:417536/1‑150 (MQ=255)
                                                       aCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATaaa                                                                                                >  2:1683366/1‑149 (MQ=255)
                                                       aCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTGGTATCAGGTGGAAAAATaaa                                                                                                >  2:1691459/1‑149 (MQ=255)
                                                               tCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaa                                                                                        >  2:1728527/1‑150 (MQ=255)
                                                                ccATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaa                                                                                       >  2:1994968/1‑150 (MQ=255)
                                                                ccATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaa                                                                                       >  2:160229/1‑150 (MQ=255)
                                                                 cATCAGAAATCACCCCAGGGTAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCACTTTCTCGTTGTATAAGGTTGAAAAATAACTATGTCaaaa                                                                                      >  2:331139/1‑150 (MQ=255)
                                                                  aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTTATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                     >  2:302536/1‑150 (MQ=255)
                                                                  aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                     >  2:146697/1‑150 (MQ=255)
                                                                  aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                     <  2:649953/150‑1 (MQ=255)
                                                                   tCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACg                                                                                    >  2:832348/1‑150 (MQ=255)
                                                                         aTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACAtt                                                                              <  2:1565915/150‑1 (MQ=255)
                                                                           cACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAATTAACTATGTCAAAACGAACATTcc                                                                            >  2:1328671/1‑149 (MQ=255)
                                                                              cccAGGGGAAGCAGACGCTATCGTTAACGTACCGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATACCGCCCTTTTACTTCCTCTTTCTCGGTGTATCAGGGTGAAAAATAACTATTTCAAAACGAACATTCGCgg                                                                         >  2:1729014/1‑150 (MQ=255)
                                                                               ccAGGGGAAGCAGACGCTATCGTAAACGTAACGCTCGATTACCTTTAATACGTGAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGGTGTATCAGGTTGAAAAATTACTATGTCATAACGAACATTCGCGGt                                                                        <  2:1303955/150‑1 (MQ=255)
                                                                                cAGGGGAAGCAGACGCTATCGTTAACGTAACGCCGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTg                                                                       <  2:1913843/150‑1 (MQ=255)
                                                                                  ggggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTCGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                     <  2:698767/150‑1 (MQ=255)
                                                                                  ggggAAGCAGACGCTATCGATAACGTAACGCTGGATTACCCTTAATACGTTAGTGCGTTATATGACCTGTCAGATAACGCCCTTTTCCTACCTATTTCTCGTTGGATAAAGTTGAAAAATAACTATGTCAAAACGAACAATCTCGGTGat                                                                     >  2:1100677/1‑150 (MQ=255)
                                                                                  ggggAAGCAGACGCAATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                     <  2:698890/150‑1 (MQ=255)
                                                                                       aGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTaa                                                                >  1:680722/1‑150 (MQ=255)
                                                                                        gCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTTACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACTAACATTCGCGGTGATATTAAc                                                               <  2:557860/150‑1 (MQ=255)
                                                                                           gACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCtt                                                            >  1:474787/1‑150 (MQ=255)
                                                                                             agCTATAGTTAACGTAACTCTGGATTACCGTTAATAAGTTAGGGAGTTATCTGACCTTTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATAAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgt                                                          <  1:177272/149‑1 (MQ=255)
                                                                                               cTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg                                                        <  2:910288/150‑1 (MQ=255)
                                                                                                   cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTGCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATTACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTgtgtt                                                    >  1:396421/1‑149 (MQ=255)
                                                                                                   cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                    <  2:756151/150‑1 (MQ=255)
                                                                                                   cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAATATGACAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                    >  1:1863433/1‑150 (MQ=255)
                                                                                                        acgtaacgCTGGATTACCGGTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTc                                               <  1:110768/150‑1 (MQ=255)
                                                                                                           taaagCTTGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGt                                            <  1:1445525/150‑1 (MQ=255)
                                                                                                             acgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTGTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAt                                          >  2:166560/1‑150 (MQ=255)
                                                                                                              cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAATATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                         >  2:1571211/1‑150 (MQ=255)
                                                                                                              cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                         >  2:1992694/1‑150 (MQ=255)
                                                                                                              cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                         <  2:1796068/150‑1 (MQ=255)
                                                                                                                gtgttttACCGTAAATACGTTAGGGCGTAATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTgg                                       <  2:1472640/145‑1 (MQ=255)
                                                                                                                gtGGTTTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTgg                                       <  1:441207/149‑1 (MQ=255)
                                                                                                                 gggATTACCGTTAATACGTTAGTGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGc                                      <  1:1755115/149‑1 (MQ=255)
                                                                                                                   gATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCa                                    <  1:1170774/150‑1 (MQ=255)
                                                                                                                       aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                                <  1:861067/150‑1 (MQ=255)
                                                                                                                       aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                                >  2:709785/1‑150 (MQ=255)
                                                                                                                       aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCACTTTCTCGTTGTATCAGGTTGAAAAAAAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                                <  2:792177/150‑1 (MQ=255)
                                                                                                                        ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGCTAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGCACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcgc                               >  1:180885/1‑150 (MQ=255)
                                                                                                                        ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTAAAAAAGAACATTAGCGGGGATATTAACATTGTTGTGTAGCTTCTGTATTGGCCAGgcgc                               >  1:701043/1‑150 (MQ=255)
                                                                                                                        ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTACTCTTTCTCGTTGTATCAGGGTGAAAAAAAACTATCTCAAAACCAACATTCGCGGTGATATTAACCTTGTTGTGTAGATTCTGTATTGGCCAGgcgc                               >  2:1272680/1‑150 (MQ=255)
                                                                                                                              aTACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCag                         >  1:1517610/1‑150 (MQ=255)
                                                                                                                                 cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTATCCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                      >  1:1838200/1‑150 (MQ=255)
                                                                                                                                 cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                      >  1:1434050/1‑150 (MQ=255)
                                                                                                                                 cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                      <  1:441147/150‑1 (MQ=255)
                                                                                                                                 cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                      <  2:1645427/150‑1 (MQ=255)
                                                                                                                                 cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGGGATATTAACCTTGTTGTGTAGCTTCTGTATTGGTCAGGCGCTGGCaggag                      >  1:464143/1‑150 (MQ=255)
                                                                                                                                  gtaacggCGTTATCTGTCCTGACAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGATAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTTGCCAGGCGCTTGCaggagg                     <  2:1258301/145‑1 (MQ=255)
                                                                                                                                  gTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggagg                     >  1:1988764/1‑150 (MQ=255)
                                                                                                                                    tAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGaa                   <  1:1557701/150‑1 (MQ=255)
                                                                                                                                       ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg                >  1:1381099/1‑150 (MQ=255)
                                                                                                                                       ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg                >  2:490112/1‑150 (MQ=255)
                                                                                                                                       ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg                >  1:632144/1‑150 (MQ=255)
                                                                                                                                       ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTAGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg                >  2:1887908/1‑150 (MQ=255)
                                                                                                                                       ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCATCTTTCTCGTTTTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCg                >  2:974576/1‑150 (MQ=255)
                                                                                                                                        gCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt               >  2:1702832/1‑150 (MQ=255)
                                                                                                                                          gTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt             <  2:1607283/150‑1 (MQ=255)
                                                                                                                                            tATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAACTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTa           >  1:1964127/1‑150 (MQ=255)
                                                                                                                                             ttCTGACATGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAATATAACTATGTCAATCCGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTAc          <  1:104429/149‑1 (MQ=255)
                                                                                                                                              tCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa         >  2:1412316/1‑150 (MQ=255)
                                                                                                                                              tCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa         >  1:1879923/1‑150 (MQ=255)
                                                                                                                                                tGACCTGTCAGATAACGCCCTTTTCCTTCCTATTTCTCGTTGTATCATGTTGAAAAATAACTATCTCAAAACGAACATTCGCGGTTATATTAAACTTGTTGAGTAGCTAATTTATATGCAATGCGCTAGCAGGAGGAATCTTTTTACTGc       >  1:729858/1‑150 (MQ=255)
                                                                                                                                                     tGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGTGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAcg  <  2:981945/150‑1 (MQ=255)
                                                                                                                                                     tGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAcg  >  1:1660331/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACG  >  NC_000913/3287884‑3288182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: