Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,307,094 T→G 10.3% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,307,0940TG10.3% 223.5 / 3.0 103N256T (AAC→ACC) deaDATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (36/52);  new base G (11/2);  total (48/55)
Fisher's exact test for biased strand distribution p-value = 5.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CTGCAACGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACT  >  NC_000913/3306945‑3307240
                                                                                                                                                     |                                                                                                                                                  
cTGCAACGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGt                                                                                                                                                    <  1:1447735/150‑1 (MQ=255)
 tGCAACGTCGGTCGCAATCAGGATGGCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt                                                                                                                                                   <  1:193123/150‑1 (MQ=255)
   cAACGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtttt                                                                                                                                                 >  2:762223/1‑150 (MQ=255)
     aCGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTg                                                                                                                                               <  2:228345/150‑1 (MQ=255)
     aCGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGATCAGCGCGGCGCTGTTGTAGCCGTTACTCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTGTTTg                                                                                                                                               >  2:1066435/1‑150 (MQ=255)
      cGTCGGTCGCAATCAGGATGTCCAGACGACCTTCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTgg                                                                                                                                              <  2:1888847/150‑1 (MQ=255)
       gtcggtcgCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt                                                                                                                                             <  2:911258/150‑1 (MQ=255)
        tcggtcgCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTa                                                                                                                                            <  1:709601/150‑1 (MQ=255)
           gtcgCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacga                                                                                                                                         <  1:1362203/150‑1 (MQ=255)
             cgCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgatc                                                                                                                                       >  2:1230763/1‑148 (MQ=255)
              gCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTAACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTtcgaacg                                                                                                                                      >  2:1491873/1‑150 (MQ=255)
               cAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGGaccaacga                                                                                                                                     >  1:1018887/1‑150 (MQ=255)
                aaTCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa                                                                                                                                    >  2:1854221/1‑150 (MQ=255)
                 aTCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGACTGTTCACGCAGCGCCTGGTGCATGTCCCCGTTCAGCCCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCCGCCACTTCCACAGTCGCGTTTTAGGTAcgaacgaac                                                                                                                                   >  1:960707/1‑149 (MQ=255)
                 aTCAGGATGTCCAGACGACCATCTTTCAGGCGTGCCAGTGTCTCTTCACGCAGCGCCTGGTTCATGGCTCCGTTCAGCTCGGCGCTGTTGTAGCCGTTACGCTGAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAg                                                                                                                                   <  1:311752/150‑1 (MQ=255)
                      gATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTAGAACGAAaataat                                                                                                                              >  1:173134/1‑150 (MQ=255)
                        tGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGACGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcg                                                                                                                            <  1:223241/150‑1 (MQ=255)
                           ccAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGTTACGAACGAAGATAATcgccc                                                                                                                         >  2:1944941/1‑149 (MQ=255)
                           ccAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccg                                                                                                                         <  2:921506/150‑1 (MQ=255)
                            cAGCCGACCATCTTTCAGGCGGTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc                                                                                                                        <  1:395036/150‑1 (MQ=255)
                            aaGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTAACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCGAAATAGCTTCAGCAACTTCAAGAGTCGCGTTTTTGGTACGATAGAAGATAATcgccgc                                                                                                                        >  2:1347681/2‑150 (MQ=255)
                             aGACGACAATCTTTCAGGCGTTCCAGTGTCTGTTCCCGCAGCGCCTGGTGCATGTCACCGTTCAGCGCGGCCATGTTCTAGCCGTTACGCTCAAGAGCTACAGCCACTACCAGAGTCGCCTATTAGGAACGGACGAAGAAAATCGCCGCa                                                                                                                       >  2:788243/1‑150 (MQ=255)
                                 gacCATCTTTGAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGAGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTACAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaa                                                                                                                   <  1:54065/150‑1 (MQ=255)
                                  acCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTGCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaaa                                                                                                                  <  2:425573/150‑1 (MQ=255)
                                   ccATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCCCCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCCCTTCCAGCGTCGCGGTTTTGGTTCCGACCAAGATAATCGCCGCAACaaaa                                                                                                                 >  1:542994/1‑150 (MQ=255)
                                   ccATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaaaa                                                                                                                 >  2:238350/1‑150 (MQ=255)
                                         ttCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTGGGAACGAAGAAAAAACCCGCATCaaaaaagtgc                                                                                                           >  1:395863/1‑144 (MQ=255)
                                         ttCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTcc                                                                                                           <  1:757600/150‑1 (MQ=255)
                                           cAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGCGTCGCGTTTTTTGTACGAACGAAGATAAAAGCCGCATCAAAATCTTACGc                                                                                                         >  1:459570/1‑150 (MQ=255)
                                            aGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGCGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt                                                                                                        >  2:1297252/1‑150 (MQ=255)
                                            aGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGGACGAACGAAGATAATAGCCGCATCAAAATCTTCCGcg                                                                                                        >  2:603673/1‑149 (MQ=255)
                                                  tCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAgg                                                                                                  <  1:637593/150‑1 (MQ=255)
                                                         tCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa                                                                                           <  1:158315/150‑1 (MQ=255)
                                                           tGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTAcc                                                                                         <  1:1512544/150‑1 (MQ=255)
                                                             ttCACGCAGCGCCTGGTGTATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAg                                                                                       <  2:510916/150‑1 (MQ=255)
                                                               cACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATATTCCGCTTCCAGGAAACGGACCAGTg                                                                                     >  2:1586507/1‑150 (MQ=255)
                                                               cACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGAACGAAGAAAATCGCCGCATCAAAAACTTACGCTTCCAGGAAACGTACAAGTg                                                                                     >  2:1448152/1‑150 (MQ=255)
                                                                aCGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCCGCCACTTCCAGCGTCGCGGGTTTGGTACGAACGCAGATAAACGCCGCCTCAAAATCTGCCGCGTACAGGAAACGTCCCAGTGc                                                                                    >  2:1216442/1‑150 (MQ=255)
                                                                aCGCAGCGCCTGGTTCATGTCACCGATCAGCGCGGCGCTGTAGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTATTTGGTACGAACGAAAATAATAGCAGCAACAAACTCTTCCGCTTCAATGAAACGTACCATTGc                                                                                    >  1:244771/1‑150 (MQ=255)
                                                                 cGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAAAGAAGATAATAGCCGCAGCAAAAACTGACCCATCCCGGAAAAATACCactagt                                                                                   >  2:1925646/1‑145 (MQ=255)
                                                                    aGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCg                                                                                <  2:676723/150‑1 (MQ=255)
                                                                    aGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTTGTACGAACGAAGATAATAGCCGCCACAAAATCTTCCGCGTGCAGGAAACGTACCACTGCTTCg                                                                                >  2:650303/1‑150 (MQ=255)
                                                                     gcgcCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCTTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGt                                                                               <  2:960707/150‑1 (MQ=255)
                                                                       gcCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCTCTTCCAGGAAACGTACCAGTGCTTCGttt                                                                             <  1:39637/150‑1 (MQ=255)
                                                                       gcCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGttt                                                                             >  1:239115/1‑150 (MQ=255)
                                                                       gcCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGCACGAAGATAATAGCCGCCGCAAAATCTTCCGCTTCCAGGAAACGGACCAGTGCTGGGttt                                                                             >  2:1444217/1‑150 (MQ=255)
                                                                        ccTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGtttt                                                                            <  2:1478255/150‑1 (MQ=255)
                                                                          tGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTg                                                                          <  1:1919385/150‑1 (MQ=255)
                                                                          gtgTTCATGTCACCGTTCAGCTCGGCGCTGTTGTTGCGGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCTTTGCTTCGTTTTTg                                                                          <  1:129485/148‑1 (MQ=255)
                                                                           ggTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTgc                                                                         <  2:606173/150‑1 (MQ=255)
                                                                           ggTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTgc                                                                         <  2:614231/150‑1 (MQ=255)
                                                                           ggTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGATTTTGGTGCGGGCGAAGATAATCGCCGCATCAAAATCTTACGCTTCCCGGAAACGTACCAGTGCATCGGTTTTgg                                                                         >  1:759971/1‑149 (MQ=255)
                                                                           ggTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGATTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTgc                                                                         <  2:1383506/150‑1 (MQ=255)
                                                                            gTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCATCCAGGAAACGTACCAGTGCTTCGTTTTTgcg                                                                        <  1:475758/150‑1 (MQ=255)
                                                                            gTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAAATAATCGCCGCATAAAAATCTTACGCTTCCAGGAAACGTACCAGGGGGTCGTTTTTgcg                                                                        >  1:367445/1‑150 (MQ=255)
                                                                             ttCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTgcgc                                                                       <  2:231262/150‑1 (MQ=255)
                                                                             ttCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGCACGAAGATAATCGCCGCCTCAAAATCTTACGCGTCGAGGCAACGTACCCGTGGTTCGTTTTTgcgc                                                                       >  1:200785/1‑150 (MQ=255)
                                                                              tCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATAGACGCATCAAAATCTTCAGCTTCCAGGTAAGGTACCAGTGGGTGGTTTTTgcgcg                                                                      >  1:383738/1‑149 (MQ=255)
                                                                                aTGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATa                                                                    >  1:1157814/1‑150 (MQ=255)
                                                                                 tGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATAc                                                                   >  1:75179/1‑150 (MQ=255)
                                                                                 tGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCGTCCAGGAAACGTACCAGTGCTTAGTTTTTGCGCATAc                                                                   >  1:732276/1‑150 (MQ=255)
                                                                                    cACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATAcccc                                                                >  1:1165045/1‑150 (MQ=255)
                                                                                    cACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGTCGCACGAAGATAAAAGCCGCATCAAAATATTACGCTTCCCGGAAACGTACCACGGCGTAGTTTTTGAGCATAcccc                                                                >  1:1621042/1‑150 (MQ=255)
                                                                                     aCCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCa                                                               <  1:1195125/150‑1 (MQ=255)
                                                                                          tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCCTACCCCAGACAg                                                          >  1:1285790/1‑150 (MQ=255)
                                                                                          tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGTCGCACGAAGAGAATAGCCGCCTCCAAATCTTACGCTTCCCGGAAACGGACCAGTGCTTCGTTTTTGGGGCTACCCCAGGCAg                                                          >  2:1828328/1‑150 (MQ=255)
                                                                                            aGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAACCACTTCCAGAGTCGCGGTTTTGGTTCGAACGAATATAATCGCCGCATCAAAATCTTACGCTTCCAGGGAACCTACCAGTGCGTCGTTTTTTCGCATACCCCAGCCAGTc                                                        >  2:1512287/1‑150 (MQ=255)
                                                                                             gcgcGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCATAAAAATATTACGCTTACAGGAAAAGTACCAGTGCTTAGGTTTTGCGGATACCCCAGCCAATcc                                                       >  2:1557710/1‑150 (MQ=255)
                                                                                             gcgcGGCGCTGTTGTAGCCGTTACGCGCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTcc                                                       <  2:586335/150‑1 (MQ=255)
                                                                                              cgcgGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGCCAGTCCa                                                      >  1:226275/1‑150 (MQ=255)
                                                                                               gcggcgCTGTTGTATCCGTTAAGCTCAATAGGATCATCCAATTCCAGTTTCGGGTTTTTGGTACGTACGAATATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCATCGTTTTTGCGCATACCCCAGACAGTCCAg                                                     <  2:175852/150‑1 (MQ=255)
                                                                                                cggcgCTGTTGTAGCCGTTACTCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGt                                                    <  1:124857/150‑1 (MQ=255)
                                                                                                 ggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTa                                                   <  2:1928773/150‑1 (MQ=255)
                                                                                                    gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGct                                                <  2:1952300/150‑1 (MQ=255)
                                                                                                       gttgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTg                                             <  1:1191285/150‑1 (MQ=255)
                                                                                                         tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGc                                           <  1:635214/150‑1 (MQ=255)
                                                                                                         tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGc                                           <  2:470561/150‑1 (MQ=255)
                                                                                                             gCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGAt                                       <  1:927686/150‑1 (MQ=255)
                                                                                                              ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATg                                      <  1:921742/150‑1 (MQ=255)
                                                                                                                 ttACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCTTCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCa                                   <  2:69705/150‑1 (MQ=255)
                                                                                                                  tACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAg                                  <  1:1978724/150‑1 (MQ=255)
                                                                                                                    cGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTAGAACGAAGATAATAGCCGCATCAAAATCGTACGCTTGCAGGGAACGTACCAGTGTTTCGGTTTTGCGCAAACCCCCGACAGGCCAGGAGCTCGGGCGGTTGTGAggg                                >  2:1463105/1‑149 (MQ=255)
                                                                                                                        cAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATCTTACGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTTCGCAAACCCCACACAGTCCAGTAGCGCTGGCTGATGTCAGGACggg                            >  2:598620/1‑150 (MQ=255)
                                                                                                                             gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTc                       <  1:544535/150‑1 (MQ=255)
                                                                                                                             gCTTCAGCCACTTCCAGAGTCGCGGTTTTGTTACGCACGAAGATAATAGCCGCCTCAAAATCTTACGCTTGCAGGAAACCTACCAGTGCGTCGTTTTTTCGCATACCCCAGACACTCCAGTAGCTCTGGCTGATGTAAGGACGGGTAGTc                       >  2:584100/1‑150 (MQ=255)
                                                                                                                                tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACg                    <  1:1557710/150‑1 (MQ=255)
                                                                                                                                 cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGc                   <  1:718204/150‑1 (MQ=255)
                                                                                                                                  aGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCt                  <  1:232635/150‑1 (MQ=255)
                                                                                                                                   tccACTTCCTGAGGCGCGTTTTTGGTACGAAAGAAGATAATCGGCGGATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTg                 <  2:244771/149‑1 (MQ=255)
                                                                                                                                   gCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTg                 <  1:1364450/150‑1 (MQ=255)
                                                                                                                                   gCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTAGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTg                 >  1:138956/1‑150 (MQ=255)
                                                                                                                                    ccACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCACCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTgg                <  2:1445398/150‑1 (MQ=255)
                                                                                                                                     cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTACGCTTGCAGGAAACGTACCCGTGGTTAGTTTTTTGGCCTACCCCCGACACTGCAGTAGGTCTGGGTGATGGGAGGAAGGGTAGTCACGCgggg               >  2:660689/1‑149 (MQ=255)
                                                                                                                                      aCTTCCAGAGTCGCGGTTTTGGTACGAACGAAGAAAATCGCCGCATCAAAATCTTCCGCCTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCCTACCCCAGACAGGCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGAc              <  2:1814651/150‑1 (MQ=255)
                                                                                                                                        atCCCGAGTCGCGTTGTTGGTCCGAACGAAGATAATCGCCGCCTCAAACTCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTg            <  2:1573577/149‑1 (MQ=255)
                                                                                                                                         tCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGa           >  1:1387090/1‑150 (MQ=255)
                                                                                                                                          ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCAGGACTGaa          >  1:1147859/1‑150 (MQ=255)
                                                                                                                                           cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAACTCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAAt         <  2:200460/150‑1 (MQ=255)
                                                                                                                                            agagTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATg        <  1:762223/150‑1 (MQ=255)
                                                                                                                                                  gcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGATTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCAca  >  2:80929/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTGCAACGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTTTGCGCATACCCCAGACAGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACT  >  NC_000913/3306945‑3307240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: