Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,382,133 T→G 5.4% intergenic (+23/‑67) degQ → / → degS serine endoprotease, periplasmic/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,382,1330TG 5.4% 420.9 / 10.5 142intergenic (+23/‑67)degQ/degSserine endoprotease, periplasmic/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (65/69);  new base G (5/3);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

Only 100 of 143 total aligned reads displayed.

CATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGC  >  NC_000913/3381984‑3382280
                                                                                                                                                     |                                                                                                                                                    
cATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                                                                                                                      <  2:1076470/150‑1 (MQ=255)
   tGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTAc                                                                                                                                                   <  1:972513/150‑1 (MQ=255)
   tGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGAAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTAc                                                                                                                                                   >  1:381093/1‑150 (MQ=255)
    ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGATGATGCGTTAATGACGTAAACCGGGCAACAGGCTTACg                                                                                                                                                  >  2:131320/1‑150 (MQ=255)
       gTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGTGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCAGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgtgt                                                                                                                                               <  2:854444/150‑1 (MQ=255)
        tCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGtgtg                                                                                                                                              <  1:47897/150‑1 (MQ=255)
         cAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCTTAAACCGGGCTTCAGGCTTACttgggt                                                                                                                                             >  2:1945094/1‑147 (MQ=255)
         cAACCGCGATCGGGTGAACTCGATTGCTGAAATGAGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGa                                                                                                                                             <  2:289435/150‑1 (MQ=255)
           aCCGCGAGCTGGTGAACTCGATTGCGGAAATGCGTACAGGGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTACACCGGGCATCAGGCTTACGTGTGATg                                                                                                                                           <  2:1051024/150‑1 (MQ=255)
                 aTCGGGTGAACTCGATTGCTGAAATGCGTAAAGGGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGt                                                                                                                                     <  2:1411787/150‑1 (MQ=255)
                  tCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                                                                                                                    <  1:213564/150‑1 (MQ=255)
                  tCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                                                                                                                    <  1:478255/150‑1 (MQ=255)
                   cggtagAACTCGATTGCTGAAATTGGTAAAGTGCTGTCGGCAAAACCGGGCATCATCGCCCTGCAAATTGTAGGCGGCATTGTAAGCATCTATCTCCTGATGCGTTAATGTCGTAAACCGGGCATCTGTCTTACGTGTGATGTCCGGTTa                                                                                                                                   <  1:321383/145‑1 (MQ=255)
                   cGGGTGAACTCGTTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACAGGGCATCAGGCTTACGTGTGATGTCAGGTTa                                                                                                                                   >  2:726244/1‑150 (MQ=255)
                   cGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTa                                                                                                                                   <  2:1416845/150‑1 (MQ=255)
                   cGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCACACGTAAGCCTGATGCCCGGttt                                                                                                                                   <  2:27245/150‑2 (MQ=255)
                      gTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACt                                                                                                                                <  2:1749945/150‑1 (MQ=255)
                         aaCTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGt                                                                                                                             >  2:1427049/1‑150 (MQ=255)
                            tCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                                                                                                          <  2:1576203/150‑1 (MQ=255)
                            tCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                                                                                                          <  2:1815433/150‑1 (MQ=255)
                               aTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGACCTGCAAATTGTACGCGGCAATGAAAGCATATATCTGCTGATGAGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtg                                                                                                                       >  1:426998/1‑150 (MQ=255)
                               aTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCACCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtg                                                                                                                       <  1:1290925/150‑1 (MQ=255)
                                  gCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctg                                                                                                                    >  2:1866266/1‑150 (MQ=255)
                                     gAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                                                                                                                 >  1:537333/1‑150 (MQ=255)
                                     gAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                                                                                                                 >  1:719707/1‑150 (MQ=255)
                                          gCGTAAAGTGCTGGCTGCAAAACAGTCCATCATAGCCCTGCAAATTGTACGCGGCAATTAAAGAATCTATCTGCTGATGCGTTAATGTCGTAAACAGGGAATCATGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGtt                                                                                                            <  1:1305562/150‑1 (MQ=255)
                                          gCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGtt                                                                                                            >  1:1534115/1‑150 (MQ=255)
                                          gCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTAGCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCggt                                                                                                            >  1:1333663/1‑148 (MQ=255)
                                           cGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAATCCGGGCATCAGGCTTACGTGTGATGTGCGGTTAACTCGTGGTATGCTGCTGCCGTTc                                                                                                           >  2:1360341/1‑150 (MQ=255)
                                             tAAAGTGCGGGCGGCAAAACCGGCCATCATCGCCCTGCAAATAGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTccc                                                                                                         <  2:470805/150‑1 (MQ=255)
                                             cacaGTGCGGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCACTGAAAGCATCTATCTGCTGCTGCGTTCATGTCGTAAACCGGGCAGCAGGCTTACGGGTGCAGTCCGGTTAACTCGTGGTATGCTGCAGCCGTTccc                                                                                                         <  1:244017/147‑1 (MQ=255)
                                                    cTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTa                                                                                                 <  1:923586/150‑1 (MQ=255)
                                                     tGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTaa                                                                                                >  1:526749/1‑150 (MQ=255)
                                                      ggcggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAAt                                                                                               >  2:1076935/1‑150 (MQ=255)
                                                        ctgcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGa                                                                                             <  1:1862316/148‑1 (MQ=255)
                                                        cggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGa                                                                                             >  1:386555/1‑150 (MQ=255)
                                                         ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc                                                                                            >  1:1263005/1‑150 (MQ=255)
                                                         ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGATGCCGTTCCCTTTTTTAATGAc                                                                                            >  1:1182023/1‑150 (MQ=255)
                                                          gcAAAACCGGCCATCCTCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGGGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACg                                                                                           <  2:132757/150‑1 (MQ=255)
                                                          gcAAAACCGGCCATCAACGCCCTGCAAATTCTACGCGGCAATGAAAGCATCTAACTGCTGATGCGGTAATTTCGTAAACCGGTCATCAGGCTTATGTGTGATGTCCGGTTAACTCGAGCTATGCTGCTGCCGTTCCCTTATTTAATGagg                                                                                           >  1:1330630/1‑148 (MQ=255)
                                                           cAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACgg                                                                                          >  1:1216305/1‑149 (MQ=255)
                                                            aaaaCCGGCCATCATCGCACTGCAAATTGTACGCGGCAAGGAAAGCATCGATCTGCTGATGCGTTAATGGCGTAAACCGGTCAGCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGcc                                                                                         <  1:1820471/150‑1 (MQ=255)
                                                             aaaCCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCt                                                                                        >  2:508856/1‑150 (MQ=255)
                                                              aaCCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTc                                                                                       >  2:740978/1‑150 (MQ=255)
                                                                ccGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAACCCGGGCATCCGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCca                                                                                     <  1:436072/150‑1 (MQ=255)
                                                                 cGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcat                                                                                    >  1:795050/1‑150 (MQ=255)
                                                                 cGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcat                                                                                    >  1:1960081/1‑150 (MQ=255)
                                                                  ggCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCATGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatc                                                                                   <  1:835748/150‑1 (MQ=255)
                                                                   gCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                  <  1:557041/150‑1 (MQ=255)
                                                                   gCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                  <  1:215383/150‑1 (MQ=255)
                                                                   gCCATCAGCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCGGGCTTACGTGTGATGTCCGGTTAACTCGGGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                  <  1:984857/150‑1 (MQ=255)
                                                                    ccATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAAATCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatcat                                                                                 >  2:731032/1‑150 (MQ=255)
                                                                      atcatcGCCCTGCAAATTGTACGCGGCAAAGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGt                                                                               <  2:339588/150‑1 (MQ=255)
                                                                        catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGttt                                                                             >  1:1639841/1‑150 (MQ=255)
                                                                        catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGttt                                                                             >  1:1954364/1‑150 (MQ=255)
                                                                         atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGTGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                            <  1:414836/150‑1 (MQ=255)
                                                                         atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                            <  2:537333/150‑1 (MQ=255)
                                                                         atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                            >  1:25941/1‑150 (MQ=255)
                                                                           cGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTATTTAATGACGCCTCCATCATGTTtgtg                                                                          >  2:704970/1‑150 (MQ=255)
                                                                            gCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGa                                                                         >  2:1495588/1‑150 (MQ=255)
                                                                             cccTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGaa                                                                        <  2:567126/150‑1 (MQ=255)
                                                                              ccTGCAAATTGTACGCGGAAATGAAAGCATATATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGTGTTACGTGTGATGTCCTGTTAACTCGTGGTATTCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAg                                                                       <  2:956523/150‑1 (MQ=255)
                                                                                tGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGct                                                                     >  2:904515/1‑150 (MQ=255)
                                                                                 gCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGctc                                                                    >  2:1361945/1‑150 (MQ=255)
                                                                                     aTTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGGCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTAc                                                                <  1:1457156/150‑1 (MQ=255)
                                                                                              ggCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCATCCATCATGTTTGTGAAGCTCTTACGTTCCGTTg                                                       >  1:679601/1‑150 (MQ=255)
                                                                                                 aaTGAAAGAATATATCTGCTGATGCTTTAATGTCGTTAACCGGGCATCAGGCTTACGTGTGATGTCCTGTTAACTCGTTGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGa                                                    <  2:426998/150‑1 (MQ=255)
                                                                                                  aTGAAACCATCTATCTGCTGATTCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGAt                                                   <  1:1945094/150‑1 (MQ=255)
                                                                                                    gAAAGCATCTATCTGCTGATGCTTTAATGTCTGAAACCGGGCATCAGGCTTACGTGTGATGTGCGGTTAACTCGTGGTATGCTGCTGACGTTCCATTTTTTAATGTCGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                 <  2:1003811/150‑1 (MQ=255)
                                                                                                    gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                 <  1:1763175/150‑1 (MQ=255)
                                                                                                    gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGGGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                 <  1:1107776/150‑1 (MQ=255)
                                                                                                     aaaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTgg                                                <  2:1954364/150‑1 (MQ=255)
                                                                                                       agcagcTATCTGCTGATGCGTTAAGTGCGTCACCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTAGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGTTTGGAt                                              <  1:106686/150‑1 (MQ=255)
                                                                                                       aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGAt                                              <  2:381093/150‑1 (MQ=255)
                                                                                                                 tgctgATGCGTTAATGTCGTAAACCGGGCAGCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCgg                                    <  1:1410462/150‑1 (MQ=255)
                                                                                                                  gctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACCTTACGTTGCGATTGGATTATTTGTAGgc                                   >  2:69687/1‑150 (MQ=255)
                                                                                                                     gATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCt                                >  2:1737299/1‑150 (MQ=255)
                                                                                                                      aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTa                               <  2:1660082/150‑1 (MQ=255)
                                                                                                                         cGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTc                            >  1:740616/1‑150 (MQ=255)
                                                                                                                              aTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTctgctg                       <  2:916082/150‑1 (MQ=255)
                                                                                                                                  cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                   >  2:940819/1‑150 (MQ=255)
                                                                                                                                  cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                   >  1:884472/1‑150 (MQ=255)
                                                                                                                                  cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                   >  1:1782717/1‑150 (MQ=255)
                                                                                                                                  agttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                   >  2:1410852/5‑150 (MQ=38)
                                                                                                                                  agttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTGTTTTAATGAAGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGAGATTGGATTAATTGTCGTCGCTATTCTGCTGGTTg                   >  1:1005549/5‑150 (MQ=37)
                                                                                                                                   gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTATTTGTCGGCGCTATTCTGCTTGTTGc                  >  1:742382/1‑150 (MQ=255)
                                                                                                                                   gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGc                  >  1:1119009/1‑150 (MQ=255)
                                                                                                                                     aaaCCGGGCATCAGGCTTACGTGTGAGGTCCGGTTAACTCGTGGTATGCTGCAGCCGTGCCCTTTTTTAATGACGCCTCAATCATGTTTGTGACGCTCTTACGTTCCGTTGCGATTTGATTAATTGTCGGCGCTATTCTGCTGGTTGCCa                >  1:1531671/1‑150 (MQ=255)
                                                                                                                                      aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCAt               <  1:175085/150‑1 (MQ=255)
                                                                                                                                        ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGc             >  1:734521/1‑150 (MQ=255)
                                                                                                                                        ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTGTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGc             >  2:1390751/1‑150 (MQ=255)
                                                                                                                                         cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTAAGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGcc            >  2:79107/1‑150 (MQ=255)
                                                                                                                                              aTCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc       <  2:576402/150‑1 (MQ=255)
                                                                                                                                              aTCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc       >  2:474107/1‑150 (MQ=255)
                                                                                                                                               tCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc       <  2:1087578/150‑1 (MQ=255)
                                                                                                                                               tCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCt      >  2:635091/1‑150 (MQ=255)
                                                                                                                                                cAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTg     <  1:1866266/150‑1 (MQ=255)
                                                                                                                                                  ggCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgc  >  1:731580/1‑149 (MQ=255)
                                                                                                                                                   gCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgcgc  <  1:1076935/150‑1 (MQ=255)
                                                                                                                                                   gCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTGTTAATGACGCCTCCATCATCTTAGTCCAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGAGCTATTCTGCTGTTTGCCATGCCTTCGCTgcgc  >  1:820975/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGC  >  NC_000913/3381984‑3382280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: