Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,382,136 C→A 5.5% intergenic (+26/‑64) degQ → / → degS serine endoprotease, periplasmic/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,382,1360CA 5.5% 411.0 / 8.8 141intergenic (+26/‑64)degQ/degSserine endoprotease, periplasmic/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (64/69);  new base A (4/3);  total (69/72)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

Only 100 of 143 total aligned reads displayed.

TGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGCC  >  NC_000913/3381987‑3382284
                                                                                                                                                     |                                                                                                                                                     
tGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTAc                                                                                                                                                       <  1:972513/150‑1 (MQ=255)
tGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGAAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTAc                                                                                                                                                       >  1:381093/1‑150 (MQ=255)
 ggCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGATGATGCGTTAATGACGTAAACCGGGCAACAGGCTTACg                                                                                                                                                      >  2:131320/1‑150 (MQ=255)
    gTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGTGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCAGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgtgt                                                                                                                                                   <  2:854444/150‑1 (MQ=255)
     tCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGtgtg                                                                                                                                                  <  1:47897/150‑1 (MQ=255)
      cAACCGCGATCGGGTGAACTCGATTGCTGAAATGAGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGa                                                                                                                                                 <  2:289435/150‑1 (MQ=255)
        aCCGCGAGCTGGTGAACTCGATTGCGGAAATGCGTACAGGGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTACACCGGGCATCAGGCTTACGTGTGATg                                                                                                                                               <  2:1051024/150‑1 (MQ=255)
              aTCGGGTGAACTCGATTGCTGAAATGCGTAAAGGGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGt                                                                                                                                         <  2:1411787/150‑1 (MQ=255)
               tCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                                                                                                                        <  1:478255/150‑1 (MQ=255)
               tCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                                                                                                                        <  1:213564/150‑1 (MQ=255)
                cggtagAACTCGATTGCTGAAATTGGTAAAGTGCTGTCGGCAAAACCGGGCATCATCGCCCTGCAAATTGTAGGCGGCATTGTAAGCATCTATCTCCTGATGCGTTAATGTCGTAAACCGGGCATCTGTCTTACGTGTGATGTCCGGTTa                                                                                                                                       <  1:321383/145‑1 (MQ=255)
                cGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTa                                                                                                                                       <  2:1416845/150‑1 (MQ=255)
                  ggTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGTCAATGAAAGCATCTATCTGCAGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAAc                                                                                                                                     <  2:1216574/150‑1 (MQ=255)
                   gTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACt                                                                                                                                    <  2:1749945/150‑1 (MQ=255)
                         tCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                                                                                                              <  2:1576203/150‑1 (MQ=255)
                            aTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGACCTGCAAATTGTACGCGGCAATGAAAGCATATATCTGCTGATGAGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtg                                                                                                                           >  1:426998/1‑150 (MQ=255)
                            aTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCACCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtg                                                                                                                           <  1:1290925/150‑1 (MQ=255)
                               gCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctg                                                                                                                        >  2:1866266/1‑150 (MQ=255)
                                  gAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                                                                                                                     >  1:719707/1‑150 (MQ=255)
                                  gAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                                                                                                                     >  1:537333/1‑150 (MQ=255)
                                       gCGTAAAGTGCTGGCTGCAAAACAGTCCATCATAGCCCTGCAAATTGTACGCGGCAATTAAAGAATCTATCTGCTGATGCGTTAATGTCGTAAACAGGGAATCATGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGtt                                                                                                                <  1:1305562/150‑1 (MQ=255)
                                       gCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGtt                                                                                                                >  1:1534115/1‑150 (MQ=255)
                                       gCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTAGCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCggt                                                                                                                >  1:1333663/1‑148 (MQ=255)
                                        cGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAATCCGGGCATCAGGCTTACGTGTGATGTGCGGTTAACTCGTGGTATGCTGCTGCCGTTc                                                                                                               >  2:1360341/1‑150 (MQ=255)
                                          cacaGTGCGGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCACTGAAAGCATCTATCTGCTGCTGCGTTCATGTCGTAAACCGGGCAGCAGGCTTACGGGTGCAGTCCGGTTAACTCGTGGTATGCTGCAGCCGTTccc                                                                                                             <  1:244017/147‑1 (MQ=255)
                                              gTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtttt                                                                                                        <  2:1369864/150‑1 (MQ=255)
                                                gCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtttttt                                                                                                      <  2:782235/150‑1 (MQ=255)
                                                 cTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTa                                                                                                     <  1:923586/150‑1 (MQ=255)
                                                 cTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGAATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACACGTGGTATGCTGCTGCCGTTCCCTATTTTa                                                                                                     >  2:570248/1‑150 (MQ=255)
                                                  tGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTaa                                                                                                    >  1:526749/1‑150 (MQ=255)
                                                     ctgcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGa                                                                                                 <  1:1862316/148‑1 (MQ=255)
                                                     cggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGa                                                                                                 >  1:386555/1‑150 (MQ=255)
                                                      ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc                                                                                                >  1:1263005/1‑150 (MQ=255)
                                                      ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc                                                                                                >  2:712739/1‑150 (MQ=255)
                                                      ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGATGCCGTTCCCTTTTTTAATGAc                                                                                                >  1:1182023/1‑150 (MQ=255)
                                                       gcAAAACCGGCCATCAACGCCCTGCAAATTCTACGCGGCAATGAAAGCATCTAACTGCTGATGCGGTAATTTCGTAAACCGGTCATCAGGCTTATGTGTGATGTCCGGTTAACTCGAGCTATGCTGCTGCCGTTCCCTTATTTAATGagg                                                                                               >  1:1330630/1‑148 (MQ=255)
                                                        cAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACgg                                                                                              >  1:1216305/1‑149 (MQ=255)
                                                         aaaaCCGGCCATCATCGCACTGCAAATTGTACGCGGCAAGGAAAGCATCGATCTGCTGATGCGTTAATGGCGTAAACCGGTCAGCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGcc                                                                                             <  1:1820471/150‑1 (MQ=255)
                                                           aaCCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTc                                                                                           >  2:740978/1‑150 (MQ=255)
                                                             ccGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAACCCGGGCATCCGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCca                                                                                         <  1:436072/150‑1 (MQ=255)
                                                              cGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcat                                                                                        >  1:1960081/1‑150 (MQ=255)
                                                              cGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcat                                                                                        >  1:795050/1‑150 (MQ=255)
                                                               ggCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCATGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatc                                                                                       <  1:835748/150‑1 (MQ=255)
                                                                gCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                      <  1:215383/150‑1 (MQ=255)
                                                                gCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                      <  1:557041/150‑1 (MQ=255)
                                                                gCCATCAGCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCGGGCTTACGTGTGATGTCCGGTTAACTCGGGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                      <  1:984857/150‑1 (MQ=255)
                                                                 ccATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAAATCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatcat                                                                                     >  2:731032/1‑150 (MQ=255)
                                                                   atcatcGCCCTGCAAATTGTACGCGGCAAAGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGt                                                                                   <  2:339588/150‑1 (MQ=255)
                                                                    tcatcGCCCTGCAAATTGTACGCGGCAATGAAAGCTTCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGtt                                                                                  <  2:500996/150‑1 (MQ=255)
                                                                     catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGttt                                                                                 >  1:1639841/1‑150 (MQ=255)
                                                                     catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGttt                                                                                 >  1:1954364/1‑150 (MQ=255)
                                                                      atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGTGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                                <  1:414836/150‑1 (MQ=255)
                                                                      atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                                <  2:537333/150‑1 (MQ=255)
                                                                      atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                                >  1:25941/1‑150 (MQ=255)
                                                                        cGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTATTTAATGACGCCTCCATCATGTTtgtg                                                                              >  2:704970/1‑150 (MQ=255)
                                                                          cccTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGaa                                                                            <  2:567126/150‑1 (MQ=255)
                                                                           ccTGCAAATTGTACGCGGAAATGAAAGCATATATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGTGTTACGTGTGATGTCCTGTTAACTCGTGGTATTCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAg                                                                           <  2:956523/150‑1 (MQ=255)
                                                                             tGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGct                                                                         >  2:904515/1‑150 (MQ=255)
                                                                                  aTTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGGCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTAc                                                                    <  1:1457156/150‑1 (MQ=255)
                                                                                           ggCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCATCCATCATGTTTGTGAAGCTCTTACGTTCCGTTg                                                           >  1:679601/1‑150 (MQ=255)
                                                                                              aaTGAAAGAATATATCTGCTGATGCTTTAATGTCGTTAACCGGGCATCAGGCTTACGTGTGATGTCCTGTTAACTCGTTGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGa                                                        <  2:426998/150‑1 (MQ=255)
                                                                                               aTGAAACCATCTATCTGCTGATTCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGAt                                                       <  1:1945094/150‑1 (MQ=255)
                                                                                                 gAAAGCATCTATCTGCTGATGCTTTAATGTCTGAAACCGGGCATCAGGCTTACGTGTGATGTGCGGTTAACTCGTGGTATGCTGCTGACGTTCCATTTTTTAATGTCGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                     <  2:1003811/150‑1 (MQ=255)
                                                                                                 gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                     <  1:1763175/150‑1 (MQ=255)
                                                                                                 gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGGGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                     <  1:1107776/150‑1 (MQ=255)
                                                                                                  aaaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTgg                                                    <  2:1954364/150‑1 (MQ=255)
                                                                                                   aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGa                                                   <  2:1411945/150‑1 (MQ=255)
                                                                                                    agcagcTATCTGCTGATGCGTTAAGTGCGTCACCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTAGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGTTTGGAt                                                  <  1:106686/150‑1 (MQ=255)
                                                                                                     gCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGAtt                                                 <  2:1986519/150‑1 (MQ=255)
                                                                                                             ctgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTTCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCg                                         <  2:194048/150‑1 (MQ=255)
                                                                                                             ctgctgATGCGTTAATGTCATAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCg                                         >  2:443213/1‑150 (MQ=255)
                                                                                                              tgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCgg                                        >  2:1440564/1‑150 (MQ=255)
                                                                                                              tgctgATGCGTTAATGTCGTAAACCGGGCAGCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCgg                                        <  1:1410462/150‑1 (MQ=255)
                                                                                                                  gATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCt                                    >  2:1737299/1‑150 (MQ=255)
                                                                                                                      cGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTc                                >  1:740616/1‑150 (MQ=255)
                                                                                                                           aTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTctgctg                           <  2:916082/150‑1 (MQ=255)
                                                                                                                            tGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTgg                          <  2:1768058/150‑1 (MQ=255)
                                                                                                                               cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                       >  2:940819/1‑150 (MQ=255)
                                                                                                                               cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                       >  1:1782717/1‑150 (MQ=255)
                                                                                                                               cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                       >  1:884472/1‑150 (MQ=255)
                                                                                                                               agttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTGTTTTAATGAAGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGAGATTGGATTAATTGTCGTCGCTATTCTGCTGGTTg                       >  1:1005549/5‑150 (MQ=37)
                                                                                                                                gttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGc                      >  2:696661/4‑150 (MQ=38)
                                                                                                                                gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTATTTGTCGGCGCTATTCTGCTTGTTGc                      >  1:742382/1‑150 (MQ=255)
                                                                                                                                gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGc                      >  1:1119009/1‑150 (MQ=255)
                                                                                                                                 tAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGcc                     >  2:348308/1‑150 (MQ=255)
                                                                                                                                  aaaCCGGGCATCAGGCTTACGTGTGAGGTCCGGTTAACTCGTGGTATGCTGCAGCCGTGCCCTTTTTTAATGACGCCTCAATCATGTTTGTGACGCTCTTACGTTCCGTTGCGATTTGATTAATTGTCGGCGCTATTCTGCTGGTTGCCa                    >  1:1531671/1‑150 (MQ=255)
                                                                                                                                   aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCAt                   <  1:175085/150‑1 (MQ=255)
                                                                                                                                     ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGc                 >  1:734521/1‑150 (MQ=255)
                                                                                                                                     ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTGTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGc                 >  2:1390751/1‑150 (MQ=255)
                                                                                                                                      cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGcc                <  2:1609191/150‑1 (MQ=255)
                                                                                                                                      cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTAAGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGcc                >  2:79107/1‑150 (MQ=255)
                                                                                                                                           aTCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc           >  2:474107/1‑150 (MQ=255)
                                                                                                                                            tCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc           <  2:1087578/150‑1 (MQ=255)
                                                                                                                                            tCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCt          >  2:635091/1‑150 (MQ=255)
                                                                                                                                             cAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTg         <  1:1866266/150‑1 (MQ=255)
                                                                                                                                              aGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgc        <  2:1347544/150‑1 (MQ=255)
                                                                                                                                               ggCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgca       >  1:731580/1‑149 (MQ=255)
                                                                                                                                                gCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgcgc      <  1:1076935/150‑1 (MQ=255)
                                                                                                                                                gCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTGTTAATGACGCCTCCATCATCTTAGTCCAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGAGCTATTCTGCTGTTTGCCATGCCTTCGCTgcgc      >  1:820975/1‑150 (MQ=255)
                                                                                                                                                    aCGTGGGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATGGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGcc  <  2:1442826/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGCC  >  NC_000913/3381987‑3382284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: