Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,382,139 G→C 5.1% intergenic (+29/‑61) degQ → / → degS serine endoprotease, periplasmic/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,382,1390GC 5.1% 455.9 / 12.1 137intergenic (+29/‑61)degQ/degSserine endoprotease, periplasmic/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (63/67);  new base C (4/3);  total (67/70)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 142 total aligned reads displayed.

GTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGCCTTA  >  NC_000913/3381991‑3382287
                                                                                                                                                    |                                                                                                                                                     
gTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGTGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCAGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgtgt                                                                                                                                                      <  2:854444/150‑1 (MQ=255)
 tCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGtgtg                                                                                                                                                     <  1:47897/150‑1 (MQ=255)
  cAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCTTAAACCGGGCTTCAGGCTTACttgggt                                                                                                                                                    >  2:1945094/1‑147 (MQ=255)
  cAACCGCGATCGGGTGAACTCGATTGCTGAAATGAGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGa                                                                                                                                                    <  2:289435/150‑1 (MQ=255)
   aaCCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGGTAATGTCGTAAACCGGGCCTCAGGCTTACGTGTGAt                                                                                                                                                   >  1:1768058/1‑150 (MQ=255)
    aCCGCGAGCTGGTGAACTCGATTGCGGAAATGCGTACAGGGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTACACCGGGCATCAGGCTTACGTGTGATg                                                                                                                                                  <  2:1051024/150‑1 (MQ=255)
     ccGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGt                                                                                                                                                 >  1:1233803/1‑150 (MQ=255)
          aTCGGGTGAACTCGATTGCTGAAATGCGTAAAGGGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGt                                                                                                                                            <  2:1411787/150‑1 (MQ=255)
           tCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                                                                                                                           <  1:478255/150‑1 (MQ=255)
            cggtagAACTCGATTGCTGAAATTGGTAAAGTGCTGTCGGCAAAACCGGGCATCATCGCCCTGCAAATTGTAGGCGGCATTGTAAGCATCTATCTCCTGATGCGTTAATGTCGTAAACCGGGCATCTGTCTTACGTGTGATGTCCGGTTa                                                                                                                                          <  1:321383/145‑1 (MQ=255)
             gggTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGACATCACACGTAAGCCTGATGCCCGGttta                                                                                                                                         <  1:512717/150‑3 (MQ=38)
               gTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACt                                                                                                                                       <  2:1749945/150‑1 (MQ=255)
                tGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGACATCAGACGTAAGCCTGATGCCCGGTTTAcga                                                                                                                                      <  1:1838078/150‑3 (MQ=38)
                  aaCTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGt                                                                                                                                    >  2:1427049/1‑150 (MQ=255)
                     tCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                                                                                                                 <  2:1815433/150‑1 (MQ=255)
                     tCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                                                                                                                 <  2:1576203/150‑1 (MQ=255)
                        aTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGACCTGCAAATTGTACGCGGCAATGAAAGCATATATCTGCTGATGAGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtg                                                                                                                              >  1:426998/1‑150 (MQ=255)
                        aTTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCACCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtg                                                                                                                              <  1:1290925/150‑1 (MQ=255)
                           gCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctg                                                                                                                           >  2:1866266/1‑150 (MQ=255)
                              gAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                                                                                                                        >  1:537333/1‑150 (MQ=255)
                              gAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                                                                                                                        >  1:719707/1‑150 (MQ=255)
                                   gCGTAAAGTGCTGGCTGCAAAACAGTCCATCATAGCCCTGCAAATTGTACGCGGCAATTAAAGAATCTATCTGCTGATGCGTTAATGTCGTAAACAGGGAATCATGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGtt                                                                                                                   <  1:1305562/150‑1 (MQ=255)
                                   gCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGtt                                                                                                                   >  1:1534115/1‑150 (MQ=255)
                                   gCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTAGCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCggt                                                                                                                   >  1:1333663/1‑148 (MQ=255)
                                      tAAAGTGCGGGCGGCAAAACCGGCCATCATCGCCCTGCAAATAGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTccc                                                                                                                <  2:470805/150‑1 (MQ=255)
                                          gTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtttt                                                                                                           <  2:1369864/150‑1 (MQ=255)
                                             cTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTa                                                                                                        <  1:923586/150‑1 (MQ=255)
                                               ggcggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAAt                                                                                                      >  2:1076935/1‑150 (MQ=255)
                                                 cggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGa                                                                                                    >  1:386555/1‑150 (MQ=255)
                                                  ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc                                                                                                   >  2:712739/1‑150 (MQ=255)
                                                  ggcAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGATGCCGTTCCCTTTTTTAATGAc                                                                                                   >  1:1182023/1‑150 (MQ=255)
                                                   gcAAAACCGGCCATCCTCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGGGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACg                                                                                                  <  2:132757/150‑1 (MQ=255)
                                                   gcAAAACCGGCCATCAACGCCCTGCAAATTCTACGCGGCAATGAAAGCATCTAACTGCTGATGCGGTAATTTCGTAAACCGGTCATCAGGCTTATGTGTGATGTCCGGTTAACTCGAGCTATGCTGCTGCCGTTCCCTTATTTAATGagg                                                                                                  >  1:1330630/1‑148 (MQ=255)
                                                    cAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACgg                                                                                                 >  1:1216305/1‑149 (MQ=255)
                                                     aaaaCCGGCCATCATCGCACTGCAAATTGTACGCGGCAAGGAAAGCATCGATCTGCTGATGCGTTAATGGCGTAAACCGGTCAGCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGcc                                                                                                <  1:1820471/150‑1 (MQ=255)
                                                       aaCCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTc                                                                                              >  2:740978/1‑150 (MQ=255)
                                                         ccGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCca                                                                                            <  1:428730/150‑1 (MQ=255)
                                                          cGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcat                                                                                           >  1:1960081/1‑150 (MQ=255)
                                                          cGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcat                                                                                           >  1:795050/1‑150 (MQ=255)
                                                           ggCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCATGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatc                                                                                          <  1:835748/150‑1 (MQ=255)
                                                            gCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                         <  1:215383/150‑1 (MQ=255)
                                                            gCCATCAGCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCGGGCTTACGTGTGATGTCCGGTTAACTCGGGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatca                                                                                         <  1:984857/150‑1 (MQ=255)
                                                             ccATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAAATCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCcatcat                                                                                        >  2:731032/1‑150 (MQ=255)
                                                               atcatcGCCCTGCAAATTGTACGCGGCAAAGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGt                                                                                      <  2:339588/150‑1 (MQ=255)
                                                                tcatcGCCCTGCAAATTGTACGCGGCAATGAAAGCTTCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGtt                                                                                     <  2:500996/150‑1 (MQ=255)
                                                                 catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGttt                                                                                    >  1:1639841/1‑150 (MQ=255)
                                                                 catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGttt                                                                                    >  1:1954364/1‑150 (MQ=255)
                                                                  atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                                   <  2:537333/150‑1 (MQ=255)
                                                                  atcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTtg                                                                                   >  1:25941/1‑150 (MQ=255)
                                                                    cGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTATTTAATGACGCCTCCATCATGTTtgtg                                                                                 >  2:704970/1‑150 (MQ=255)
                                                                     gCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGa                                                                                >  2:1495588/1‑150 (MQ=255)
                                                                      cccTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGaa                                                                               <  2:567126/150‑1 (MQ=255)
                                                                       ccTGCAAATTGTACGCGGAAATGAAAGCATATATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGTGTTACGTGTGATGTCCTGTTAACTCGTGGTATTCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAg                                                                              <  2:956523/150‑1 (MQ=255)
                                                                         tGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGct                                                                            >  2:904515/1‑150 (MQ=255)
                                                                          gCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGctc                                                                           >  2:1361945/1‑150 (MQ=255)
                                                                            aaaTTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCtt                                                                         >  1:399335/1‑150 (MQ=255)
                                                                              aTTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGGCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTAc                                                                       <  1:1457156/150‑1 (MQ=255)
                                                                                  tACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCATCCATCATGTTTGTGAAGCTCTTACGtta                                                                   >  1:1491982/1‑149 (MQ=255)
                                                                                       ggCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCATCCATCATGTTTGTGAAGCTCTTACGTTCCGTTg                                                              >  1:679601/1‑150 (MQ=255)
                                                                                          aaTGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGa                                                           >  1:543124/1‑150 (MQ=255)
                                                                                          aaTGAAAGAATATATCTGCTGATGCTTTAATGTCGTTAACCGGGCATCAGGCTTACGTGTGATGTCCTGTTAACTCGTTGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGa                                                           <  2:426998/150‑1 (MQ=255)
                                                                                           aTGAAACCATCTATCTGCTGATTCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGAt                                                          <  1:1945094/150‑1 (MQ=255)
                                                                                             gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                        <  1:1763175/150‑1 (MQ=255)
                                                                                             gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGGGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTg                                                        <  1:1107776/150‑1 (MQ=255)
                                                                                              aaaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTgg                                                       <  2:1954364/150‑1 (MQ=255)
                                                                                               aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGa                                                      <  2:1411945/150‑1 (MQ=255)
                                                                                                agcagcTATCTGCTGATGCGTTAAGTGCGTCACCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTAGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGTTTGGAt                                                     <  1:106686/150‑1 (MQ=255)
                                                                                                aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGAt                                                     <  2:381093/150‑1 (MQ=255)
                                                                                                 gCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGAtt                                                    <  2:1986519/150‑1 (MQ=255)
                                                                                                   atctttctGTTGTTGCGTTAATGTCGTAAACCGGGGATCATGCTTACGGGTGATGGCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGAATaa                                                  <  1:209704/150‑1 (MQ=255)
                                                                                                          tgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCgg                                           >  2:1440564/1‑150 (MQ=255)
                                                                                                          tgctgATGCGTTAATGTCGTAAACCGGGCAGCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCgg                                           <  1:1410462/150‑1 (MQ=255)
                                                                                                              gATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCt                                       >  2:1737299/1‑150 (MQ=255)
                                                                                                                  cGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTc                                   >  1:374620/1‑150 (MQ=255)
                                                                                                                  cGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTc                                   >  1:740616/1‑150 (MQ=255)
                                                                                                                       aTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTctgctg                              <  2:916082/150‑1 (MQ=255)
                                                                                                                           cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                          >  2:940819/1‑150 (MQ=255)
                                                                                                                           cGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                          >  1:884472/1‑150 (MQ=255)
                                                                                                                           agttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                          >  2:1410852/5‑150 (MQ=38)
                                                                                                                           agttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTGTTTTAATGAAGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGAGATTGGATTAATTGTCGTCGCTATTCTGCTGGTTg                          >  1:1005549/5‑150 (MQ=37)
                                                                                                                           agttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCGTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGGGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTg                          >  1:998861/5‑150 (MQ=37)
                                                                                                                            gttaaCCGGACATCACACGTAAGCCTGATGCCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGc                         >  2:696661/4‑150 (MQ=38)
                                                                                                                            gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTATTTGTCGGCGCTATTCTGCTTGTTGc                         >  1:742382/1‑150 (MQ=255)
                                                                                                                            gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGc                         >  1:1119009/1‑150 (MQ=255)
                                                                                                                               aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCAt                      <  1:175085/150‑1 (MQ=255)
                                                                                                                                 ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGc                    >  1:734521/1‑150 (MQ=255)
                                                                                                                                  cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGcc                   <  2:1609191/150‑1 (MQ=255)
                                                                                                                                  cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGcc                   <  1:231302/150‑1 (MQ=255)
                                                                                                                                  cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTAAGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGcc                   >  2:79107/1‑150 (MQ=255)
                                                                                                                                       aTCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc              <  2:576402/150‑1 (MQ=255)
                                                                                                                                       aTCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc              >  2:474107/1‑150 (MQ=255)
                                                                                                                                        tCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGc              <  2:1087578/150‑1 (MQ=255)
                                                                                                                                        tCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCt             >  2:635091/1‑150 (MQ=255)
                                                                                                                                         cAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTg            <  1:1866266/150‑1 (MQ=255)
                                                                                                                                          aGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgc           <  2:1347544/150‑1 (MQ=255)
                                                                                                                                           ggCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgca          >  1:731580/1‑149 (MQ=255)
                                                                                                                                            gCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTgcgc         <  1:1076935/150‑1 (MQ=255)
                                                                                                                                            gCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTGTTAATGACGCCTCCATCATCTTAGTCCAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGAGCTATTCTGCTGTTTGCCATGCCTTCGCTgcgc         >  1:820975/1‑150 (MQ=255)
                                                                                                                                                aCGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGcc     <  2:788167/150‑1 (MQ=255)
                                                                                                                                                   tgtgATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGACTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGCCTTa  >  1:592995/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGCCTCCATCATGTTTGTGAAGCTCTTACGTTCCGTTGCGATTGGATTAATTGTCGGCGCTATTCTGCTGGTTGCCATGCCTTCGCTGCGCAGCCTTA  >  NC_000913/3381991‑3382287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: