Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,388,284 A→T 5.3% A281A (GCT→GCA aaeA ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,388,2840AT 5.3% 363.3 / 5.2 119A281A (GCT→GCAaaeAp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base A (45/67);  new base T (5/2);  total (50/69)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCC  >  NC_000913/3388135‑3388433
                                                                                                                                                     |                                                                                                                                                     
cGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTa                                                                                                                                                       <  1:925420/150‑1 (MQ=255)
  ccAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGc                                                                                                                                                     <  2:1279085/150‑1 (MQ=255)
  ccAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGc                                                                                                                                                     <  1:1469612/150‑1 (MQ=255)
     gTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAgt                                                                                                                                                  <  1:982310/150‑1 (MQ=255)
         tACCTCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCACGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAgtggtg                                                                                                                                              <  2:1862737/150‑1 (MQ=255)
            cGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCCAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGccc                                                                                                                                           >  1:1028444/1‑150 (MQ=255)
             gCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGTCAGTGCCCACCACTGTTGCAGTGTTGCCCg                                                                                                                                          >  1:188108/1‑150 (MQ=255)
             gCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCg                                                                                                                                          >  2:565076/1‑150 (MQ=255)
             gCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCg                                                                                                                                          >  1:1620356/1‑150 (MQ=255)
               aaaGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCa                                                                                                                                        <  2:1824138/150‑1 (MQ=255)
                aaGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAg                                                                                                                                       >  1:346759/1‑150 (MQ=255)
                 aGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAgg                                                                                                                                      <  2:204894/150‑1 (MQ=255)
                 aGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAgg                                                                                                                                      <  2:1512738/150‑1 (MQ=255)
                 aGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAgg                                                                                                                                      <  2:1349588/150‑1 (MQ=255)
                   cGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGcc                                                                                                                                    <  1:519287/150‑1 (MQ=255)
                   cGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGGGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGcc                                                                                                                                    <  2:500282/150‑1 (MQ=255)
                         tGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCAGTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGt                                                                                                                              >  1:1277149/1‑150 (MQ=255)
                                  gCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCGCTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCt                                                                                                                     >  2:121821/1‑150 (MQ=255)
                                  gCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCt                                                                                                                     >  1:1960169/1‑150 (MQ=255)
                                   cAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGTGATGGGACATTTTACGGAAGAACGAATCCTGGCTTTCGACGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGACCGCAGGCCACAAGTTCTCTTGCTg                                                                                                                    >  1:1601390/1‑150 (MQ=255)
                                    aaTGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTgg                                                                                                                   >  2:808200/1‑150 (MQ=255)
                                      tGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGtt                                                                                                                 <  1:188639/150‑1 (MQ=255)
                                       ggAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTg                                                                                                                <  2:1337727/150‑1 (MQ=255)
                                       ggAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTg                                                                                                                >  2:1596431/1‑150 (MQ=255)
                                          gaAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCg                                                                                                             <  1:1584285/150‑1 (MQ=255)
                                          gaAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCg                                                                                                             >  1:1516285/1‑150 (MQ=255)
                                            aaaTACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCgag                                                                                                           <  1:369502/150‑1 (MQ=255)
                                              aTACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTGTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGAc                                                                                                         <  1:1952343/150‑1 (MQ=255)
                                               tACCCATCGTGATTATCCATAGTCAGGCAGGCTTTGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGTTCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTGTTGCTGGTTGTCGAGACg                                                                                                        <  2:798030/150‑1 (MQ=255)
                                               tACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACg                                                                                                        >  2:1267363/1‑150 (MQ=255)
                                                 cccTTCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGaa                                                                                                      <  1:538665/150‑1 (MQ=255)
                                                   cATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGCAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATa                                                                                                    <  1:1386148/150‑1 (MQ=255)
                                                    aTCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATAc                                                                                                   <  1:1043466/150‑1 (MQ=255)
                                                      cGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGa                                                                                                 >  1:96545/1‑150 (MQ=255)
                                                        ttaTTAACCAAACTCACGCATGCGATTGGCCATTATACGGAAGAACGAATCCTGGCTTTCGTCGCGATATTGATTGCCAGTGGCCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGaaa                                                                                               <  1:315395/148‑2 (MQ=255)
                                                          aTTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCg                                                                                             <  2:1172528/150‑1 (MQ=255)
                                                           ttAACCAAACTCACGCAGGCGATTGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg                                                                                            <  1:423868/150‑1 (MQ=255)
                                                           ttAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGACCCgg                                                                                            >  1:1976926/1‑150 (MQ=255)
                                                           ttAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg                                                                                            <  2:1620630/150‑1 (MQ=255)
                                                            tAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGa                                                                                           <  2:1762323/150‑1 (MQ=255)
                                                            aaaCCAAACTCCCGCAGGCGATCGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGa                                                                                           <  2:308810/149‑1 (MQ=255)
                                                              aCCAATCTCACGCAGGCGATGGGCCATTTTTCGGAAGAACGAATCCTGGCTTTCGGCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGGCGAGACGAATACGAACCGGAac                                                                                         <  2:1798131/150‑1 (MQ=255)
                                                              aCCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAac                                                                                         >  1:1251221/1‑150 (MQ=255)
                                                              aCCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAac                                                                                         <  2:966446/150‑1 (MQ=255)
                                                              aCCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCACAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAac                                                                                         >  1:893134/1‑150 (MQ=255)
                                                               ccAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAaca                                                                                        >  1:1779804/1‑150 (MQ=255)
                                                                     tCACGCCGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGACGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgc                                                                                  <  2:265695/150‑1 (MQ=255)
                                                                      cACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgcg                                                                                 >  1:1473100/1‑150 (MQ=255)
                                                                       aCGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgcgc                                                                                <  2:1529622/150‑1 (MQ=255)
                                                                        cGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGACCACGTTGCGCa                                                                               >  2:1259060/1‑150 (MQ=255)
                                                                          cAGGCGATGGGCCATTTTACTTAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGTCTAATACGAACCGGAACACGTTGCGCAAg                                                                             <  2:1946210/150‑1 (MQ=255)
                                                                          cAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGATTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCATATGTTCTCTTGCTGGTTGTAGAGACGAATACGAACAGGCACACGATGCGCaac                                                                             >  1:1471321/1‑149 (MQ=255)
                                                                            ggCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGAc                                                                           <  1:1082853/150‑1 (MQ=255)
                                                                                   ggCCATTTTACGGCAGAACGAATCCTGGCTCTCGTCGCCATCTTGAGTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGGCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCa                                                                    <  2:1036328/150‑1 (MQ=255)
                                                                                     ccATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCAtt                                                                  <  2:573154/150‑1 (MQ=255)
                                                                                        ttttACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACTAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCaa                                                               <  1:765986/150‑1 (MQ=255)
                                                                                            aCGGAAGGACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGtt                                                           >  1:1319818/1‑150 (MQ=255)
                                                                                            aCGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGtt                                                           <  2:74318/150‑1 (MQ=255)
                                                                                             cGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa                                                          >  2:702911/1‑150 (MQ=255)
                                                                                               gaagaaCGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTaga                                                        >  2:678238/1‑150 (MQ=255)
                                                                                                  gaaCGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTc                                                     <  1:565076/150‑1 (MQ=255)
                                                                                                          ccTGGCTTTCGTCGCGAACTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCg                                             <  2:1734078/150‑1 (MQ=255)
                                                                                                           cTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGc                                            >  1:514391/1‑150 (MQ=255)
                                                                                                           cTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGc                                            >  1:82614/1‑150 (MQ=255)
                                                                                                           cTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAATCTATAGTCGc                                            >  2:1198585/1‑150 (MQ=255)
                                                                                                           cTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGGCGAGACGAATACGAACCGGCGCCCGTTGCGCAAGCCGCACCCATTCAAGGTGAGAGTCGATAGTCGc                                            >  2:185737/1‑150 (MQ=255)
                                                                                                           cTGGCTTTCGTCGCGATCTTGATTGACAGTGACCACCACTGTAGCAGTGGTGCCCGGAGGGAAGGTGTTCTCTTGCTGGTTGTAGAGAAGAATACTAAACGGAGCACGGTGCGCAGGGCGGTCCCCTTCATGGGTTGAGGCTCGAGTCgg                                            >  2:308028/1‑149 (MQ=255)
                                                                                                             ggCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGTCGAATACGACCCGGAACACGTTGCGCAAGACGCCCCCATTCAAGGTTAGAGTCTATAGTCGCCa                                          >  2:1479998/1‑150 (MQ=255)
                                                                                                             ggCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCa                                          >  2:1259226/1‑150 (MQ=255)
                                                                                                             ggCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCAAGAGACGCCa                                          >  2:945134/1‑150 (MQ=255)
                                                                                                                     tcgCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCTGATGTTCTCTTGCTGGTGGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTg                                  <  2:944816/150‑1 (MQ=255)
                                                                                                                     tcgCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTg                                  <  2:558006/150‑1 (MQ=255)
                                                                                                                     tcgCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTg                                  <  2:1117343/150‑1 (MQ=255)
                                                                                                                        cgATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACCCGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcg                               >  2:1412477/1‑150 (MQ=255)
                                                                                                                         gATCTTGTTTGCCAGTGTCCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgt                              <  2:645946/150‑1 (MQ=255)
                                                                                                                         gATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgt                              <  2:346759/150‑1 (MQ=255)
                                                                                                                         gATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCAGCAGGCAAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGATCCGGAACACGTTGCGCAAGACGCTCCCATTCAAGGTTAGGGTCTATAGTCGCCTTCCCTTTgtcgt                              >  1:347763/1‑150 (MQ=255)
                                                                                                                          aTCTTGTTTGCCAGTGACCACCACTGTTGCAGTGGTGCCCGCAGGCCAGATGTGCTCTGGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCGATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgtc                             <  2:289288/150‑1 (MQ=255)
                                                                                                                             ttGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCAcg                          <  2:1112035/150‑1 (MQ=255)
                                                                                                                               gTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCAcgcg                        <  2:1277149/150‑1 (MQ=255)
                                                                                                                               gTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCACGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTAAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCAagcg                        >  2:1729698/1‑150 (MQ=255)
                                                                                                                                 ttGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGtg                      <  1:219389/150‑1 (MQ=255)
                                                                                                                                  tGCCAGTGACCACCACTGTATCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCCTTCAAGGGTAGAGTCTATAGTCGCCATCCCTGTGTCGTCACGCGtgc                     >  1:356220/1‑150 (MQ=255)
                                                                                                                                      aGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTgctgct                 <  1:705180/150‑1 (MQ=255)
                                                                                                                                         gggcaccacTGCTACAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGc              >  2:760289/4‑150 (MQ=255)
                                                                                                                                         gACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGTTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGc              >  2:1901627/1‑150 (MQ=255)
                                                                                                                                          ggcaccacTGCTACAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCg             >  1:543687/3‑150 (MQ=255)
                                                                                                                                          ggcaccacTGCTACAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCg             >  1:1333251/3‑150 (MQ=255)
                                                                                                                                          ggcaccacTGCTACAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCg             >  1:1385355/3‑150 (MQ=255)
                                                                                                                                          accaccACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCg             <  1:1187391/150‑1 (MQ=255)
                                                                                                                                           ccaccaCTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTGTGTCGTCACGCGTGCTGCTGGCGt            >  1:104627/1‑150 (MQ=255)
                                                                                                                                              ccacTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTgg         <  2:1960169/150‑1 (MQ=255)
                                                                                                                                               cacTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGt        <  1:1676500/150‑1 (MQ=255)
                                                                                                                                               cacTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGt        <  1:678238/150‑1 (MQ=255)
                                                                                                                                                acTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTg       >  2:1637199/1‑150 (MQ=255)
                                                                                                                                                  tGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAc     <  2:1516285/150‑1 (MQ=255)
                                                                                                                                                  tGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAc     <  1:1876623/150‑1 (MQ=255)
                                                                                                                                                   gTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGccc    >  2:138797/1‑150 (MQ=255)
                                                                                                                                                   gTAGCAGTGGTGCCCGCAGGCCAGATGTGCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCCTCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAcc    >  1:1227940/1‑150 (MQ=255)
                                                                                                                                                     aGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGCTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGGCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAcccc  <  2:96545/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCC  >  NC_000913/3388135‑3388433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: