Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,599,755 C→A 6.3% intergenic (‑97/+174) livJ ← / ← rpoH branched‑chain amino acid ABC transporter periplasmic binding protein/RNA polymerase, sigma 32 (sigma H) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,599,7550CA 6.3% 255.9 / 4.3 90intergenic (‑97/+174)livJ/rpoHbranched‑chain amino acid ABC transporter periplasmic binding protein/RNA polymerase, sigma 32 (sigma H) factor
Reads supporting (aligned to +/- strand):  ref base C (43/41);  new base A (4/2);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATC  >  NC_000913/3599606‑3599902
                                                                                                                                                     |                                                                                                                                                   
cTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTagcagc                                                                                                                                                     <  2:1873712/150‑1 (MQ=255)
    aTGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATAc                                                                                                                                                 <  1:622986/150‑1 (MQ=255)
     tGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATAct                                                                                                                                                >  1:607194/1‑150 (MQ=255)
      gCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActc                                                                                                                                               >  1:1046703/1‑150 (MQ=255)
       ccAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTACGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActct                                                                                                                                              >  1:968224/1‑150 (MQ=255)
        cAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTg                                                                                                                                             >  1:1074304/1‑150 (MQ=255)
         aGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGc                                                                                                                                            <  1:237557/150‑1 (MQ=255)
          gcgcGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCt                                                                                                                                           <  1:1423615/150‑1 (MQ=255)
          gcgcGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACACGTGCTTTAGCAGCATACTCTGCt                                                                                                                                           >  2:1010056/1‑150 (MQ=255)
           cgcgATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTa                                                                                                                                          <  2:418147/150‑1 (MQ=255)
               aTACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAAc                                                                                                                                      <  2:391477/150‑1 (MQ=255)
               aTACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGAGTATGCTGCTaaagc                                                                                                                                      <  1:451180/150‑3 (MQ=255)
                tACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACa                                                                                                                                     >  2:2007864/1‑150 (MQ=255)
                    tCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCAGGTTATTACGTGTGTTCTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATAcc                                                                                                                                 >  1:1022445/1‑150 (MQ=255)
                      cTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCg                                                                                                                               <  1:742463/150‑1 (MQ=255)
                        gCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGAt                                                                                                                             <  2:1886993/150‑1 (MQ=255)
                          cAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGAttt                                                                                                                           >  2:1150422/1‑150 (MQ=255)
                             tAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTa                                                                                                                        >  1:1858749/1‑150 (MQ=255)
                             tAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTCTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTa                                                                                                                        <  2:1743380/150‑1 (MQ=255)
                              aaCGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTAt                                                                                                                       >  2:611614/1‑150 (MQ=255)
                              aaCGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTAt                                                                                                                       <  2:1848035/150‑1 (MQ=255)
                              aaCGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTAGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTAt                                                                                                                       <  2:865723/150‑1 (MQ=255)
                                      aCCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTgg                                                                                                               <  2:455998/150‑1 (MQ=255)
                                       cccTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGa                                                                                                              <  1:519384/150‑1 (MQ=255)
                                        ccTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGaa                                                                                                             >  2:917871/1‑150 (MQ=255)
                                           ttATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAg                                                                                                          <  1:1757730/150‑1 (MQ=255)
                                               gTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAt                                                                                                      >  1:351815/1‑150 (MQ=255)
                                               gTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAt                                                                                                      >  2:562229/1‑150 (MQ=255)
                                                ttCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAtt                                                                                                     >  1:1843407/1‑150 (MQ=255)
                                                ttCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAtt                                                                                                     >  2:1737244/1‑150 (MQ=255)
                                                  cATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAtttt                                                                                                   <  2:1359467/150‑1 (MQ=255)
                                                  cATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAtttt                                                                                                   <  2:1239442/150‑1 (MQ=255)
                                                  cATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAtttt                                                                                                   <  1:1608771/150‑1 (MQ=255)
                                                  cATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTAtttt                                                                                                   <  1:842543/150‑1 (MQ=255)
                                                   aTCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTG‑TTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGa                                                                                                 >  1:1391446/1‑150 (MQ=255)
                                                    tCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGa                                                                                                 >  1:579900/1‑150 (MQ=255)
                                                     ccTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGAc                                                                                                >  1:1674628/1‑150 (MQ=255)
                                                      cTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACa                                                                                               >  2:888578/1‑150 (MQ=255)
                                                      cTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTGTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACa                                                                                               >  2:1434114/1‑150 (MQ=255)
                                                       tgtgaATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAg                                                                                              >  2:1556417/4‑150 (MQ=255)
                                                       tGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAg                                                                                              >  2:1392851/1‑150 (MQ=255)
                                                       tGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAg                                                                                              <  1:1012162/150‑1 (MQ=255)
                                                           aaTCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTtta                                                                                          >  2:1387118/1‑150 (MQ=255)
                                                             tCCCCATTCTTCTGGTTATTAGGTGTGTTGTGATGTTGTTTTTCAGCACTTTAATTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTATGACAGTttatt                                                                                        >  2:1139937/1‑150 (MQ=255)
                                                              ccccATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGCCTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTttatta                                                                                       >  2:829349/1‑150 (MQ=255)
                                                               cccATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGTATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTaa                                                                                      >  2:1947547/1‑150 (MQ=255)
                                                               cccATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTaa                                                                                      >  2:612137/1‑150 (MQ=255)
                                                               cccATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTaa                                                                                      >  2:187859/1‑150 (MQ=255)
                                                                ccATTCTTCTGGTTATTACGTGTTGTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGAATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAAc                                                                                     <  1:1371499/150‑1 (MQ=255)
                                                                    tcttctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATc                                                                                 <  1:914608/150‑1 (MQ=255)
                                                                    tcttctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATc                                                                                 <  1:1689774/150‑1 (MQ=255)
                                                                     cttctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCt                                                                                >  1:1778608/1‑150 (MQ=255)
                                                                      ttctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTg                                                                               <  2:544593/150‑1 (MQ=255)
                                                                            ttattaCGTTTGTTGTGAAGTTGTTTTTCAGCACTTTATTTCGTTTAATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGAAAGTTTATTAACAATCTGCGTggg                                                                         <  2:323934/150‑1 (MQ=255)
                                                                                  cGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTgg                                                                   >  2:1770142/1‑150 (MQ=255)
                                                                                  cGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATACATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGATATTTTGACAGTTTATTAACAATCTGCGTGGGGATTgg                                                                   >  2:254730/1‑150 (MQ=255)
                                                                                   gtgtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGc                                                                  <  1:1603342/150‑1 (MQ=255)
                                                                                       gttgtGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGttt                                                              >  1:393535/1‑150 (MQ=255)
                                                                                         tgtgATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTg                                                            <  2:903790/150‑1 (MQ=255)
                                                                                          gtgATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCAGTGCTTTAGCAGCATACTCTGCTAAAACATACAGTATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGTGGTTTGGCGtttttc                                                           >  1:358220/1‑148 (MQ=255)
                                                                                           tgATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGcc                                                          >  1:1933141/1‑150 (MQ=255)
                                                                                           tgATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGcc                                                          >  2:1682861/1‑150 (MQ=255)
                                                                                             aTGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCgg                                                        <  2:507734/150‑1 (MQ=255)
                                                                                               gttgttTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAg                                                      >  1:1612345/1‑150 (MQ=255)
                                                                                                 tgttTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAggg                                                    >  1:736685/1‑150 (MQ=255)
                                                                                                 tgttTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAggg                                                    >  2:400541/1‑150 (MQ=255)
                                                                                                  gttTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAgggg                                                   <  2:1615711/150‑1 (MQ=255)
                                                                                                       tCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGGTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAAtt                                              <  2:1074304/150‑1 (MQ=255)
                                                                                                        cAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGATATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTg                                             >  1:1512050/1‑150 (MQ=255)
                                                                                                          gCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGat                                           <  1:2007864/150‑1 (MQ=255)
                                                                                                          ccACTTTATTTCGTTTTATTCATGACTACCCGTGCTTTAGCAGCATACTCTGTTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGat                                           <  2:871049/149‑1 (MQ=255)
                                                                                                           cACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCTTGGGGATTGGCGTTTTGCCGTAGGGGAAATTGatt                                          >  2:1772148/1‑150 (MQ=255)
                                                                                                                tctttCGTTTTATGCCTGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTAc                                     <  2:884044/148‑1 (MQ=255)
                                                                                                                tATTTCGTTTTATTCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTATTGCCGGAGGGGAAATTGATTATTAc                                     <  2:298122/150‑1 (MQ=255)
                                                                                                                      gTTTTATGCATGACTACCCGTTCTTTAGCAGCATACTCTTCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGc                               <  1:1043713/150‑1 (MQ=255)
                                                                                                                         ttATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCAGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGATATTGATTATTACAGAGGCCCa                            <  1:647721/150‑1 (MQ=255)
                                                                                                                            tGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCaaaa                         >  1:397932/1‑150 (MQ=255)
                                                                                                                            tGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTGTGCCGGAGGGGAAATTGATTATTACAGAGGCCCaaaa                         >  2:1014634/1‑150 (MQ=255)
                                                                                                                             gCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCaaaaa                        <  1:917871/150‑1 (MQ=255)
                                                                                                                              cATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGACTAGCTATTTTGACAGCTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCaaaaaa                       <  2:482962/150‑1 (MQ=255)
                                                                                                                               aTGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac                      <  2:579900/150‑1 (MQ=255)
                                                                                                                               aTGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac                      <  1:1737244/150‑1 (MQ=255)
                                                                                                                                 gACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaacaa                    >  1:1141427/1‑150 (MQ=255)
                                                                                                                                   cTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaacaaaa                  <  1:1010056/150‑1 (MQ=255)
                                                                                                                                      cccGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAcc               <  2:1278072/150‑1 (MQ=255)
                                                                                                                                         atgttttAGCAGAGTATGCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCg            >  2:458215/5‑150 (MQ=255)
                                                                                                                                          tgttttAGCAGAGTATGCTGCTAAAACATACCCGATTTTTATGATATTGGAATCGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGATATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCgg           >  1:1232683/4‑150 (MQ=255)
                                                                                                                                          tgttttAGCAGAGTATGCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCgg           >  1:1476760/4‑150 (MQ=255)
                                                                                                                                          tGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCgg           <  1:1415446/150‑1 (MQ=255)
                                                                                                                                          tGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCgg           <  1:584293/150‑1 (MQ=255)
                                                                                                                                           gttttAGCAGAGTATGCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTAAATATGCCCAAAAAACAAAAACCCCGGa          >  2:350193/3‑150 (MQ=255)
                                                                                                                                                 gcagcaTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCa    >  1:1438938/1‑150 (MQ=255)
                                                                                                                                                  cagcaTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCAt   >  2:1167649/1‑150 (MQ=255)
                                                                                                                                                   agcaTACTCTGCTAAAACATTCCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATc  <  2:1402716/150‑1 (MQ=255)
                                                                                                                                                   agcaTAATCTGCTAAAACAAACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCAGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATc  >  2:1495945/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATC  >  NC_000913/3599606‑3599902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑