Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,600,993 A→G 5.1% intergenic (‑210/+35) rpoH ← / ← ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,600,9930AG 5.1% 542.8 / 13.0 178intergenic (‑210/+35)rpoH/ftsXRNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (86/80);  new base G (4/5);  total (91/87)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01

Only 100 of 183 total aligned reads displayed.

TTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTG  >  NC_000913/3600844‑3601142
                                                                                                                                                     |                                                                                                                                                     
ttAAGAGCAACGAGGTTATCATTCTCTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGa                                                                                                                                                       >  2:1450344/1‑150 (MQ=255)
 tAAGAGCACCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACTGGGTCTCTTTACCTGCTACGGaa                                                                                                                                                      >  2:971829/1‑150 (MQ=255)
  aaGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAAc                                                                                                                                                     <  1:1287245/150‑1 (MQ=255)
   agagCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAAcc                                                                                                                                                    >  2:1048686/1‑150 (MQ=255)
       cAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCAtt                                                                                                                                                >  2:674903/1‑150 (MQ=255)
        aaCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTg                                                                                                                                               <  2:102811/150‑1 (MQ=255)
         aCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGc                                                                                                                                              <  2:1867565/150‑1 (MQ=255)
         aCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTAAGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCGGCTACGGAACCCAATGc                                                                                                                                              >  1:1539360/1‑150 (MQ=255)
          cGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCa                                                                                                                                             >  1:1848634/1‑150 (MQ=255)
             ggTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAggg                                                                                                                                          >  1:256701/1‑150 (MQ=255)
               ttATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGaa                                                                                                                                        <  1:842391/150‑1 (MQ=255)
                tATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGaaa                                                                                                                                       <  2:1938016/150‑1 (MQ=255)
                 aTCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGAAag                                                                                                                                      <  2:1197736/150‑1 (MQ=255)
                   cATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAagag                                                                                                                                    <  1:414104/150‑1 (MQ=255)
                   cATTCACTGTATTATCAGACCGTGATTTTATCCACAAGTACAATGCAAGCTAGTGAATAAATTACGCACAAAATGCGACATAGAGATGAAATACCGGGAAGAGACACCGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGAAagag                                                                                                                                    <  2:1297209/150‑1 (MQ=37)
                      tCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGtat                                                                                                                                 <  1:1979612/150‑1 (MQ=255)
                       cACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGtata                                                                                                                                <  1:1118069/150‑1 (MQ=255)
                        aCTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATaa                                                                                                                               >  2:913079/1‑150 (MQ=255)
                         cTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAac                                                                                                                              >  1:1362700/1‑150 (MQ=255)
                           gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAacac                                                                                                                            >  1:594955/1‑150 (MQ=255)
                             tttATCAGACCTTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGCGAAAGAGTATAACACGc                                                                                                                          <  1:170099/150‑1 (MQ=255)
                                aTCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCttt                                                                                                                       <  2:1013840/150‑1 (MQ=255)
                                 tCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCtttt                                                                                                                      >  2:1779440/1‑150 (MQ=255)
                                   aGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttat                                                                                                                    >  1:684574/1‑150 (MQ=255)
                                      ccGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGAGGGGAAAGAGAATACCACCATTTtattat                                                                                                                 >  1:324383/1‑150 (MQ=255)
                                        gttaTTTTATCCACAAGTTCATTGGAATCTTGTGAATAAATTACGCACAAAATGTGACATAGATATTAAATACCGGGAAGAGACAAAGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGATAGAGTATAACTCGCTTTTattattg                                                                                                               <  1:149065/147‑2 (MQ=255)
                                        gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTc                                                                                                               <  2:850435/150‑1 (MQ=255)
                                         tGATTTTATCGACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTTACATAGAGATGTAATACCGGGTAGAGACAACGGGGTCTCTTTCCCTGATAATGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                              <  2:539717/150‑1 (MQ=255)
                                         tGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                              >  2:1008392/1‑150 (MQ=255)
                                         tGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                              >  2:1712580/1‑150 (MQ=255)
                                          gATTTTATCCACAAGTTCAATGCAATCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAg                                                                                                             <  2:1482785/150‑1 (MQ=255)
                                           aTTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAgg                                                                                                            >  1:1957889/1‑150 (MQ=255)
                                           aTTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAgg                                                                                                            >  1:664707/1‑150 (MQ=255)
                                            ttttATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                                           <  1:1637248/150‑1 (MQ=255)
                                             tttATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCg                                                                                                          <  1:2002080/150‑1 (MQ=255)
                                                aTCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTaa                                                                                                       <  1:1264309/150‑1 (MQ=255)
                                                   cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                    >  2:1669886/1‑150 (MQ=255)
                                                   cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                    >  2:1341083/1‑150 (MQ=255)
                                                   cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                    >  2:728962/1‑150 (MQ=255)
                                                       aGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCg                                                                                                <  2:208637/150‑1 (MQ=255)
                                                        gTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                               <  2:256701/150‑1 (MQ=255)
                                                          tCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaa                                                                                             >  2:1513108/1‑150 (MQ=255)
                                                             aTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtg                                                                                          <  1:813354/150‑1 (MQ=255)
                                                              tGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgt                                                                                         <  1:1725921/150‑1 (MQ=255)
                                                                cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                                       >  1:1037579/1‑150 (MQ=255)
                                                                   gCTTGTGAATAAATTACGCAAAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACTGAACCCATTGCAGGGAAAGAGTATAACAAGCTTTTATTATTCAGGAGTAAAGTGTCGTAAATGTTGTaa                                                                                    >  1:721380/1‑149 (MQ=255)
                                                                    cTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTAcc                                                                                   <  2:1672449/150‑1 (MQ=255)
                                                                     ttGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCg                                                                                  >  2:1790271/1‑150 (MQ=255)
                                                                      tgtgAATAAATTACGCACAAAATGTGACATAGAGATGAAATTCCGGGAAGAGACAACGGGGTCTCTTTACCTGCTACTGAACCCATTGCAGGTTAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGt                                                                                 <  1:1357041/150‑1 (MQ=255)
                                                                       gtgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTg                                                                                <  2:1865698/150‑1 (MQ=255)
                                                                        tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGTAAGAGACAACGGGGGCTCTTTCCCTGCTACGGATCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                               <  1:1599931/150‑1 (MQ=255)
                                                                        tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                               >  2:286233/1‑150 (MQ=255)
                                                                         gAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                              >  1:1327944/1‑150 (MQ=255)
                                                                             aaaTTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGc                                                                          >  2:892629/1‑150 (MQ=255)
                                                                              aaTTACGCACAAAATGTGAGATAGAGATGAAATACCGGGAAGAGACAACGGGTTCTCTTTACCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCGTGTAGTATGCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGcc                                                                         >  2:313852/1‑150 (MQ=255)
                                                                                 tACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACg                                                                      <  2:495630/150‑1 (MQ=255)
                                                                                     cacaAAAGGGGACCTCGCGATGAAATCCCGGGACGAGACAACGGGGCCTCTTTCCCTGCTACGGAACCTATTGCAGGGAAAGAGTATAACACGCTTTTATTCATCACGCGTAAAGTGGCGTAAATGTTGAACCGTGGCTTGCCACggtac                                                                  <  2:280794/150‑5 (MQ=255)
                                                                                      acaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGcc                                                                 <  1:550213/150‑1 (MQ=255)
                                                                                          aaTGTGACATAGAGATGAACTACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCAccc                                                             <  1:310660/150‑1 (MQ=255)
                                                                                             gtgACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGc                                                          >  1:1923589/1‑150 (MQ=255)
                                                                                              tgACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGcc                                                         <  2:492382/150‑1 (MQ=255)
                                                                                               gACATAGAGATGAAATACCGTGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCa                                                        <  1:485750/150‑1 (MQ=255)
                                                                                                aCATAGAGATGAAATATCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCaa                                                       <  2:984021/150‑1 (MQ=255)
                                                                                                   tAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACACATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCAACCCAGCCAatca                                                    >  1:82423/1‑150 (MQ=255)
                                                                                                     gagaTGAAATACCGGGAAGCGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAATAGTATAACCCGCTTGTATTATTCAGGGGTAAAGTGGCGGATATGTTGTCCCGTGGCACGCCACGCTGCGACCCGGCCaaggatc                                                  >  2:89108/1‑145 (MQ=255)
                                                                                                     gagaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                  <  2:605655/150‑1 (MQ=255)
                                                                                                       gaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGAACGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGAAAATGTTGTACCGTGGCAAGCAACGCTGCCACCCAGCCAATCCTCGa                                                >  1:1586980/1‑150 (MQ=255)
                                                                                                        aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                               <  1:598034/150‑1 (MQ=255)
                                                                                                        aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                               <  1:589881/150‑1 (MQ=255)
                                                                                                         ttaaaTACCGGGAAGAGACAAGGGGGTCTCTTTCCCTGCTACGGAACCCTTTGCCGGAAATTAGTAAAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAATTGTTGTACCGTGGAAAGCCAGGTTGCCACCCAGCCAATCATCGAGc                                              <  2:512438/148‑1 (MQ=255)
                                                                                                         tGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                              >  1:1808231/1‑150 (MQ=255)
                                                                                                            aaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATa                                           >  1:859286/1‑150 (MQ=255)
                                                                                                              tACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTTTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGATCATAcc                                         >  2:562483/1‑150 (MQ=255)
                                                                                                              tACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATAcc                                         >  1:604489/1‑150 (MQ=255)
                                                                                                                 ggggAAGAGACATCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAATGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagc                                      <  2:1539360/149‑1 (MQ=255)
                                                                                                                   ggAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                    <  1:892629/150‑1 (MQ=255)
                                                                                                                   ggAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                    <  2:1621920/150‑1 (MQ=255)
                                                                                                                   ggAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                    >  1:121045/1‑150 (MQ=255)
                                                                                                                    gAAGAGACAACGGGGTCTATTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCagcagc                                   <  2:107987/150‑1 (MQ=255)
                                                                                                                     aaGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAgcagca                                  <  2:594955/150‑1 (MQ=255)
                                                                                                                         gaCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTAAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCCAGCCACGCTGCCACCCAGCCAATCCTCGAGCATACCAGCAGCAATAg                              >  2:1481296/1‑150 (MQ=255)
                                                                                                                         gaCAACGGGGTCTCTTTCCCGGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCCAGCCACGCTGCCACCCAGCCATTCATCGAGCATACCAGCAGCAATAg                              >  1:1428977/1‑150 (MQ=255)
                                                                                                                           cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACCCGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                            >  1:416563/1‑150 (MQ=255)
                                                                                                                           cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCGGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                            >  1:101536/1‑150 (MQ=255)
                                                                                                                                gggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCCCCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCAt                       >  2:1837985/1‑150 (MQ=255)
                                                                                                                                     tctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                  <  1:1495329/150‑1 (MQ=255)
                                                                                                                                     tctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTATTAATTCCGGCGTAAAGTGGCGTAAATGTTGTACAGTGTCAAGCCACGCTGCCTCCCAGCCAATCATCGAGCATACCAGCAGCAATAGCTGGCATTCATc                  <  2:1380017/150‑1 (MQ=255)
                                                                                                                                      ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                 <  2:1923589/150‑1 (MQ=255)
                                                                                                                                      ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                 <  2:957723/150‑1 (MQ=255)
                                                                                                                                      ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTAGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                 >  2:306721/1‑150 (MQ=255)
                                                                                                                                      ctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAAAACACATTTATTATTCAGGAGTAAAGTGTCGTAAATGTTGTACCGTGGCAAGCCACGCTGACACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                 >  2:1103572/1‑150 (MQ=255)
                                                                                                                                       tttCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTGTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACAAGAAGCAATAGCAGGCATTCATCGa                >  2:1743264/1‑150 (MQ=255)
                                                                                                                                         tCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGGGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt              <  2:60491/150‑1 (MQ=255)
                                                                                                                                           ccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGa            >  2:696544/1‑150 (MQ=255)
                                                                                                                                               cTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCCCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAg        >  1:679712/1‑150 (MQ=255)
                                                                                                                                                tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc       <  1:1712580/150‑1 (MQ=255)
                                                                                                                                                tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc       <  1:1071194/150‑1 (MQ=255)
                                                                                                                                                 aCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGcc      >  2:421025/1‑150 (MQ=255)
                                                                                                                                                 aCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGcc      >  1:974196/1‑150 (MQ=255)
                                                                                                                                                     aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTg  <  2:324383/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTG  >  NC_000913/3600844‑3601142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: