Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,600,995 C→T 5.0% intergenic (‑212/+33) rpoH ← / ← ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,600,9950CT 5.0% 587.4 / 11.2 179intergenic (‑212/+33)rpoH/ftsXRNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (87/83);  new base T (4/5);  total (91/88)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

Only 100 of 185 total aligned reads displayed.

AAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGAT  >  NC_000913/3600846‑3601144
                                                                                                                                                     |                                                                                                                                                     
aaGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAAc                                                                                                                                                       <  1:1287245/150‑1 (MQ=255)
 agagCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAAcc                                                                                                                                                      >  2:1048686/1‑150 (MQ=255)
   agCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTAACCTGCTACGGAAccaa                                                                                                                                                    >  1:875224/1‑148 (MQ=255)
     cAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCAtt                                                                                                                                                  >  2:674903/1‑150 (MQ=255)
      aaCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTg                                                                                                                                                 <  2:102811/150‑1 (MQ=255)
       aCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGc                                                                                                                                                <  2:1867565/150‑1 (MQ=255)
       aCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTAAGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCGGCTACGGAACCCAATGc                                                                                                                                                >  1:1539360/1‑150 (MQ=255)
        cGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCa                                                                                                                                               >  1:1865698/1‑150 (MQ=255)
          aGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCCAGCTTGTGAATAAATTACGCACAAAATTTGACATAGAGAGGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAgg                                                                                                                                             <  2:1379237/150‑1 (MQ=255)
           ggTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAggg                                                                                                                                            >  1:850435/1‑150 (MQ=255)
           ggTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAggg                                                                                                                                            >  1:256701/1‑150 (MQ=255)
              tATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGaaa                                                                                                                                         <  2:1938016/150‑1 (MQ=255)
               aTCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGAAag                                                                                                                                        <  2:1197736/150‑1 (MQ=255)
                 cATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAagag                                                                                                                                      <  1:414104/150‑1 (MQ=255)
                  aTTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGAAATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACACATAGCAGGGAAAGAGt                                                                                                                                     >  2:349564/1‑150 (MQ=255)
                    tCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGtat                                                                                                                                   <  1:1979612/150‑1 (MQ=255)
                      aCTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATaa                                                                                                                                 >  2:913079/1‑150 (MQ=255)
                         gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAacac                                                                                                                              >  1:594955/1‑150 (MQ=255)
                         gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAacac                                                                                                                              <  1:830667/150‑1 (MQ=255)
                           tttATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGc                                                                                                                            <  1:607924/150‑1 (MQ=255)
                              aTCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCttt                                                                                                                         <  2:1013840/150‑1 (MQ=255)
                                  gACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttatt                                                                                                                     <  2:1570935/150‑1 (MQ=255)
                                    ccGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATATCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTtattat                                                                                                                   >  1:984021/1‑150 (MQ=255)
                                    ccGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGAGGGGAAAGAGAATACCACCATTTtattat                                                                                                                   >  1:324383/1‑150 (MQ=255)
                                      gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTc                                                                                                                 <  2:850435/150‑1 (MQ=255)
                                      gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTc                                                                                                                 >  1:550676/1‑150 (MQ=255)
                                       tGATTTTATCGACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTTACATAGAGATGTAATACCGGGTAGAGACAACGGGGTCTCTTTCCCTGATAATGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                                <  2:539717/150‑1 (MQ=255)
                                       tGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                                >  2:1712580/1‑150 (MQ=255)
                                        gATTTTATCCACAAGTTCAATGCAATCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAg                                                                                                               <  2:1482785/150‑1 (MQ=255)
                                         aTTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAgg                                                                                                              <  1:1560299/150‑1 (MQ=255)
                                           tttATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCg                                                                                                            <  1:2002080/150‑1 (MQ=255)
                                               tCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTaaa                                                                                                        >  1:907916/1‑150 (MQ=255)
                                               tCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTaaa                                                                                                        >  2:598034/1‑150 (MQ=255)
                                               tCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTaaa                                                                                                        >  2:589881/1‑150 (MQ=255)
                                                ccACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAg                                                                                                       >  2:967192/1‑150 (MQ=255)
                                                 cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                      >  2:728962/1‑150 (MQ=255)
                                                 cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                      >  2:17468/1‑150 (MQ=255)
                                                     aGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCg                                                                                                  <  1:1358877/150‑1 (MQ=255)
                                                     aGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCg                                                                                                  <  1:1317907/150‑1 (MQ=255)
                                                      gTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                                 <  2:1671030/150‑1 (MQ=255)
                                                        tCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaa                                                                                               >  2:1513108/1‑150 (MQ=255)
                                                           aTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtg                                                                                            <  1:813354/150‑1 (MQ=255)
                                                            tGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgt                                                                                           <  1:1725921/150‑1 (MQ=255)
                                                              cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                                         >  1:1037579/1‑150 (MQ=255)
                                                                 gCTTGTGAATAAATTACGCAAAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACTGAACCCATTGCAGGGAAAGAGTATAACAAGCTTTTATTATTCAGGAGTAAAGTGTCGTAAATGTTGTaa                                                                                      >  1:721380/1‑149 (MQ=255)
                                                                      tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                                 >  2:286233/1‑150 (MQ=255)
                                                                       taaTAAATTACGCACAAAATGTGACTTAGATATGAAATACCGGGCAGAGACATCGGGGTCTCTTTCCCTGCTACTGTACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                <  2:1854851/149‑1 (MQ=255)
                                                                       gAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                <  2:1807191/150‑1 (MQ=255)
                                                                       gAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                >  1:1632772/1‑150 (MQ=255)
                                                                       gAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                >  2:271592/1‑150 (MQ=255)
                                                                         aTAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCaa                                                                              >  1:680020/1‑150 (MQ=255)
                                                                           aaaTTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGc                                                                            >  2:892629/1‑150 (MQ=255)
                                                                            aaTTACGCACAAAATGTGAGATAGAGATGAAATACCGGGAAGAGACAACGGGTTCTCTTTACCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCGTGTAGTATGCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGcc                                                                           >  2:313852/1‑150 (MQ=255)
                                                                              ttACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCAc                                                                         <  1:719840/150‑1 (MQ=255)
                                                                               tACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACg                                                                        <  2:495630/150‑1 (MQ=255)
                                                                                aCGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTACCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTAAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGc                                                                       >  1:1931874/1‑150 (MQ=255)
                                                                                   cacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                    >  2:23530/1‑150 (MQ=255)
                                                                                   cacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                    >  1:1195542/1‑150 (MQ=255)
                                                                                    acaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGATGcc                                                                   >  1:472537/1‑150 (MQ=255)
                                                                                     caAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCa                                                                  <  2:1536683/150‑1 (MQ=255)
                                                                                        aaTGTGACATAGAGATGAACTACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCAccc                                                               <  1:310660/150‑1 (MQ=255)
                                                                                              aCATAGAGATGAAATATCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCaa                                                         <  2:984021/150‑1 (MQ=255)
                                                                                              aCATAGAGATGAAATACCGGGACGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGGTTTTATTATTCAGGCGTAAAGGGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCGAGCCaa                                                         <  2:874251/150‑1 (MQ=255)
                                                                                               cATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAat                                                        >  1:159888/1‑150 (MQ=255)
                                                                                                aTAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCCCCCAGCCAatc                                                       >  2:1660056/1‑150 (MQ=255)
                                                                                                   gagaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                    >  1:487137/1‑150 (MQ=255)
                                                                                                   gagaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                    <  2:605655/150‑1 (MQ=255)
                                                                                                    agaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCg                                                   <  2:146499/150‑1 (MQ=255)
                                                                                                      aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                 <  1:598034/150‑1 (MQ=255)
                                                                                                      aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                 <  1:589881/150‑1 (MQ=255)
                                                                                                       ttaaaTACCGGGAAGAGACAAGGGGGTCTCTTTCCCTGCTACGGAACCCTTTGCCGGAAATTAGTAAAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAATTGTTGTACCGTGGAAAGCCAGGTTGCCACCCAGCCAATCATCGAGc                                                <  2:512438/148‑1 (MQ=255)
                                                                                                       tGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                >  1:1808231/1‑150 (MQ=255)
                                                                                                            tACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTTTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGATCATAcc                                           >  2:562483/1‑150 (MQ=255)
                                                                                                                 ggAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                      <  1:1341083/150‑1 (MQ=255)
                                                                                                                  gAAGAGACAACGGGGTCTATTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCagcagc                                     <  2:107987/150‑1 (MQ=255)
                                                                                                                        aCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGc                               <  1:1485015/150‑1 (MQ=255)
                                                                                                                         cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCGGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                              >  1:101536/1‑150 (MQ=255)
                                                                                                                           aCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAgg                            <  1:1382551/150‑1 (MQ=255)
                                                                                                                               ggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCAtt                        <  1:1217480/150‑1 (MQ=255)
                                                                                                                                   tctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                    <  1:1495329/150‑1 (MQ=255)
                                                                                                                                   tctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                    >  1:1652620/1‑150 (MQ=39)
                                                                                                                                    ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                   <  2:1923589/150‑1 (MQ=255)
                                                                                                                                    ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTAGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                   >  2:306721/1‑150 (MQ=255)
                                                                                                                                    ctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAAAACACATTTATTATTCAGGAGTAAAGTGTCGTAAATGTTGTACCGTGGCAAGCCACGCTGACACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                   >  2:1103572/1‑150 (MQ=255)
                                                                                                                                     tttCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTGTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACAAGAAGCAATAGCAGGCATTCATCGa                  >  2:1743264/1‑150 (MQ=255)
                                                                                                                                     tttCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGa                  >  2:2013200/1‑150 (MQ=255)
                                                                                                                                      ttCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGaa                 >  1:665697/1‑150 (MQ=255)
                                                                                                                                       tCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                <  2:489776/150‑1 (MQ=255)
                                                                                                                                        cccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAtt               >  1:1826907/1‑149 (MQ=255)
                                                                                                                                        cccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATg               <  2:1784009/150‑1 (MQ=255)
                                                                                                                                         ccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGa              >  2:696544/1‑150 (MQ=255)
                                                                                                                                         ccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGa              >  2:1323252/1‑150 (MQ=255)
                                                                                                                                              tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc         <  1:1071194/150‑1 (MQ=255)
                                                                                                                                              tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc         <  1:1712580/150‑1 (MQ=255)
                                                                                                                                                   aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTg    <  2:324383/150‑1 (MQ=255)
                                                                                                                                                    aCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTCCCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGa   >  2:1232480/1‑150 (MQ=255)
                                                                                                                                                    aCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCTTTGCAAGCCACGCTGCCACCCAGCGAATCCTCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGa   <  2:95514/150‑1 (MQ=255)
                                                                                                                                                     cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGat  <  1:23530/150‑1 (MQ=255)
                                                                                                                                                     cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGat  <  1:1103570/150‑1 (MQ=255)
                                                                                                                                                     cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGat  <  1:921142/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGAT  >  NC_000913/3600846‑3601144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: