Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,000 T→A 5.5% intergenic (‑217/+28) rpoH ← / ← ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,0000TA 5.5% 547.2 / 12.5 186intergenic (‑217/+28)rpoH/ftsXRNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (86/89);  new base A (6/5);  total (92/94)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 188 total aligned reads displayed.

CAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCA  >  NC_000913/3600851‑3601148
                                                                                                                                                     |                                                                                                                                                    
  aCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGc                                                                                                                                                    <  2:1867565/150‑1 (MQ=255)
   cGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCa                                                                                                                                                   >  1:1865698/1‑150 (MQ=255)
     tggTTATCATTCACTGTTTAATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAgg                                                                                                                                                 <  2:501083/149‑1 (MQ=255)
      ggTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAggg                                                                                                                                                >  1:256701/1‑150 (MQ=255)
        ttATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGaa                                                                                                                                              <  1:842391/150‑1 (MQ=255)
         tATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGaaa                                                                                                                                             <  2:1938016/150‑1 (MQ=255)
           tCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGAAaga                                                                                                                                           <  2:1027552/150‑1 (MQ=39)
            cATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAagag                                                                                                                                          <  1:414104/150‑1 (MQ=255)
            cATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAagag                                                                                                                                          <  2:622836/150‑1 (MQ=255)
            cATTCACTGTATTATCAGACCGTGATTTTATCCACAAGTACAATGCAAGCTAGTGAATAAATTACGCACAAAATGCGACATAGAGATGAAATACCGGGAAGAGACACCGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGAAagag                                                                                                                                          <  2:1297209/150‑1 (MQ=37)
               tCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGtat                                                                                                                                       <  1:1979612/150‑1 (MQ=255)
                 aCTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATaa                                                                                                                                     >  2:913079/1‑150 (MQ=255)
                  cTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAac                                                                                                                                    >  1:1362700/1‑150 (MQ=255)
                    gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAacac                                                                                                                                  >  1:594955/1‑150 (MQ=255)
                      tttATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGc                                                                                                                                <  1:607924/150‑1 (MQ=255)
                             gACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttatt                                                                                                                         <  2:1570935/150‑1 (MQ=255)
                               ccGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTtattat                                                                                                                       >  2:1506566/1‑150 (MQ=255)
                               ccGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGAGGGGAAAGAGAATACCACCATTTtattat                                                                                                                       >  1:324383/1‑150 (MQ=255)
                                 gttaTTTTATCCACAAGTTCATTGGAATCTTGTGAATAAATTACGCACAAAATGTGACATAGATATTAAATACCGGGAAGAGACAAAGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGATAGAGTATAACTCGCTTTTattattg                                                                                                                     <  1:149065/147‑2 (MQ=255)
                                 gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTc                                                                                                                     >  1:550676/1‑150 (MQ=255)
                                  tGATTTTATCGACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTTACATAGAGATGTAATACCGGGTAGAGACAACGGGGTCTCTTTCCCTGATAATGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                                    <  2:539717/150‑1 (MQ=255)
                                  tGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                                    >  2:1712580/1‑150 (MQ=255)
                                  tGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                                    >  2:1008392/1‑150 (MQ=255)
                                   gATTTTATCCACAAGTTCAATGCAATCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAg                                                                                                                   <  2:1482785/150‑1 (MQ=255)
                                     ttttATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                                                 <  1:1637248/150‑1 (MQ=255)
                                      tttATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCg                                                                                                                <  1:2002080/150‑1 (MQ=255)
                                          tCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTaaa                                                                                                            >  1:907916/1‑150 (MQ=255)
                                           ccACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAg                                                                                                           >  2:967192/1‑150 (MQ=255)
                                            cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                          >  2:728962/1‑150 (MQ=255)
                                            cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                          >  2:17468/1‑150 (MQ=255)
                                            cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                          >  2:1341083/1‑150 (MQ=255)
                                                aGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCg                                                                                                      <  2:208637/150‑1 (MQ=255)
                                                aGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCg                                                                                                      <  1:1358877/150‑1 (MQ=255)
                                                aGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCg                                                                                                      <  1:1317907/150‑1 (MQ=255)
                                                 gTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                                     <  2:1671030/150‑1 (MQ=255)
                                                 gTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                                     <  2:256701/150‑1 (MQ=255)
                                                       tGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgt                                                                                               <  1:1725921/150‑1 (MQ=255)
                                                         cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                                             >  1:1037579/1‑150 (MQ=255)
                                                                 tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGTAAGAGACAACGGGGGCTCTTTCCCTGCTACGGATCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                                     <  1:1599931/150‑1 (MQ=255)
                                                                 tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                                     >  2:286233/1‑150 (MQ=255)
                                                                  taaTAAATTACGCACAAAATGTGACTTAGATATGAAATACCGGGCAGAGACATCGGGGTCTCTTTCCCTGCTACTGTACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                    <  2:1854851/149‑1 (MQ=255)
                                                                  gAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                    <  2:1807191/150‑1 (MQ=255)
                                                                  gAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGc                                                                                    >  2:271592/1‑150 (MQ=255)
                                                                    aTAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCaa                                                                                  >  1:680020/1‑150 (MQ=255)
                                                                      aaaTTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGc                                                                                >  2:892629/1‑150 (MQ=255)
                                                                       aaTTACGCACAAAATGTGAGATAGAGATGAAATACCGGGAAGAGACAACGGGTTCTCTTTACCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCGTGTAGTATGCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGcc                                                                               >  2:313852/1‑150 (MQ=255)
                                                                         ttACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCAc                                                                             <  1:719840/150‑1 (MQ=255)
                                                                           aCGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTACCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTAAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGc                                                                           >  1:1931874/1‑150 (MQ=255)
                                                                              cacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                        <  1:20025/150‑1 (MQ=255)
                                                                              cacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                        >  1:1195542/1‑150 (MQ=255)
                                                                               acaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGATGcc                                                                       >  1:472537/1‑150 (MQ=255)
                                                                                caAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCa                                                                      <  2:1536683/150‑1 (MQ=255)
                                                                                   aaTGTGACATAGAGATGAACTACCGGGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCAccc                                                                   <  1:310660/150‑1 (MQ=255)
                                                                                       tgACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGcc                                                               <  2:492382/150‑1 (MQ=255)
                                                                                         aCATAGAGATGAAATATCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCaa                                                             <  2:984021/150‑1 (MQ=255)
                                                                                         aCATAGAGATGAAATACCGGGACGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGGTTTTATTATTCAGGCGTAAAGGGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCGAGCCaa                                                             <  2:874251/150‑1 (MQ=255)
                                                                                           aTAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCCCCCAGCCAatc                                                           >  2:1660056/1‑150 (MQ=255)
                                                                                            tAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACACATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCAACCCAGCCAatca                                                          >  1:82423/1‑150 (MQ=255)
                                                                                              gagaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAatcatc                                                        >  1:487137/1‑150 (MQ=255)
                                                                                                gaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGAACGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGAAAATGTTGTACCGTGGCAAGCAACGCTGCCACCCAGCCAATCCTCGa                                                      >  1:1586980/1‑150 (MQ=255)
                                                                                                 aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                     <  1:589881/150‑1 (MQ=255)
                                                                                                 aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                     <  1:598034/150‑1 (MQ=255)
                                                                                                  ttaaaTACCGGGAAGAGACAAGGGGGTCTCTTTCCCTGCTACGGAACCCTTTGCCGGAAATTAGTAAAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAATTGTTGTACCGTGGAAAGCCAGGTTGCCACCCAGCCAATCATCGAGc                                                    <  2:512438/148‑1 (MQ=255)
                                                                                                  tGAAATACCGGGAAGAGACACCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                    >  2:106667/1‑150 (MQ=255)
                                                                                                     aaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATa                                                 <  2:1046757/150‑1 (MQ=255)
                                                                                                     aaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATa                                                 >  1:859286/1‑150 (MQ=255)
                                                                                                       tACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTTTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGATCATAcc                                               >  2:562483/1‑150 (MQ=255)
                                                                                                         ccGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcag                                             >  1:787010/1‑150 (MQ=255)
                                                                                                            ggAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagcag                                          <  1:1341083/150‑1 (MQ=255)
                                                                                                             gAAGAGACAACGGGGTCTATTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCagcagc                                         <  2:107987/150‑1 (MQ=255)
                                                                                                              aaGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAgcagca                                        <  2:594955/150‑1 (MQ=255)
                                                                                                                  gaCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTAAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCCAGCCACGCTGCCACCCAGCCAATCCTCGAGCATACCAGCAGCAATAg                                    >  2:1481296/1‑150 (MQ=255)
                                                                                                                  gaCAACGGGGTCTCTTTCCCGGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCCAGCCACGCTGCCACCCAGCCATTCATCGAGCATACCAGCAGCAATAg                                    >  1:1428977/1‑150 (MQ=255)
                                                                                                                    cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCGGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                                  >  1:101536/1‑150 (MQ=255)
                                                                                                                         gggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTTTACCGTGGCCAGCCCCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCCGGCAt                             >  2:1228611/1‑150 (MQ=255)
                                                                                                                         gggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTACCACCCAGCCAATCATCGAGCATACAAGCAGCAATAGCAGGCAt                             >  2:711283/1‑150 (MQ=255)
                                                                                                                          ggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCAtt                            <  1:1217480/150‑1 (MQ=255)
                                                                                                                              tctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTATTAATTCCGGCGTAAAGTGGCGTAAATGTTGTACAGTGTCAAGCCACGCTGCCTCCCAGCCAATCATCGAGCATACCAGCAGCAATAGCTGGCATTCATc                        <  2:1380017/150‑1 (MQ=255)
                                                                                                                              tctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                        >  1:1652620/1‑150 (MQ=39)
                                                                                                                               ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                       <  2:1923589/150‑1 (MQ=255)
                                                                                                                               ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTAGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                       >  2:306721/1‑150 (MQ=255)
                                                                                                                               ctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAAAACACATTTATTATTCAGGAGTAAAGTGTCGTAAATGTTGTACCGTGGCAAGCCACGCTGACACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                       >  2:1103572/1‑150 (MQ=255)
                                                                                                                                tttCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTGTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACAAGAAGCAATAGCAGGCATTCATCGa                      >  2:1743264/1‑150 (MQ=255)
                                                                                                                                 ttCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGaa                     >  1:665697/1‑150 (MQ=255)
                                                                                                                                  tCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGGGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAt                    <  2:60491/150‑1 (MQ=255)
                                                                                                                                   cccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAAtt                   >  1:1826907/1‑149 (MQ=255)
                                                                                                                                   cccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATg                   <  2:1784009/150‑1 (MQ=255)
                                                                                                                                      tGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATa                >  1:1508792/1‑150 (MQ=255)
                                                                                                                                         tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc             <  1:1071194/150‑1 (MQ=255)
                                                                                                                                         tACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGc             <  1:1712580/150‑1 (MQ=255)
                                                                                                                                          aCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGcc            >  1:974196/1‑150 (MQ=255)
                                                                                                                                          aCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGcc            >  2:421025/1‑150 (MQ=255)
                                                                                                                                             gAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCAtt         <  2:686863/150‑1 (MQ=255)
                                                                                                                                              aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTg        <  2:324383/150‑1 (MQ=255)
                                                                                                                                               aCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCTTTGCAAGCCACGCTGCCACCCAGCGAATCCTCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGa       <  2:95514/150‑1 (MQ=255)
                                                                                                                                                cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGat      <  1:1103570/150‑1 (MQ=255)
                                                                                                                                                 ccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGata     <  1:728381/150‑1 (MQ=255)
                                                                                                                                                 ccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGata     <  1:1542986/150‑1 (MQ=255)
                                                                                                                                                   aTTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATc   <  1:1779440/150‑1 (MQ=255)
                                                                                                                                                    ttGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCa  <  2:818018/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCA  >  NC_000913/3600851‑3601148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: