Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,507 G→T 7.1% R194S (CGT→AGT) ‡ ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,5070GT 7.1% 293.9 / 10.7 106R194S (CGT→AGT) ‡ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (49/48);  new base T (4/4);  total (54/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

Only 100 of 111 total aligned reads displayed.

ACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAG  >  NC_000913/3601358‑3601656
                                                                                                                                                     |                                                                                                                                                     
acTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACg                                                                                                                                                       <  1:753669/150‑1 (MQ=255)
acTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACg                                                                                                                                                       >  2:1945646/1‑150 (MQ=255)
acTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACg                                                                                                                                                       <  2:1553404/150‑1 (MQ=255)
       ccGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGc                                                                                                                                                >  2:1700618/1‑150 (MQ=255)
         gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGTCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAg                                                                                                                                              <  2:1598046/150‑1 (MQ=255)
         gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAg                                                                                                                                              <  2:1726428/150‑1 (MQ=255)
          aTGAGGAGGAACACGGCAGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTAAATCTGCGTGATACGATCACGCAGc                                                                                                                                             <  1:1262415/150‑1 (MQ=255)
           tGACGAGGAACACGGCCGCCACCCTCAACACGCCGAACATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCACTGCCGTTAAGCTGCGGGATCGTATCACGCAgat                                                                                                                                            <  1:1806635/150‑3 (MQ=255)
           tGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAgat                                                                                                                                            <  2:1973778/150‑3 (MQ=255)
           tGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCg                                                                                                                                            <  1:2000712/150‑1 (MQ=255)
           tGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCg                                                                                                                                            <  2:1545429/150‑1 (MQ=255)
           tGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCg                                                                                                                                            <  1:415410/150‑1 (MQ=255)
                 ggAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAACCCAGCTGGCATCCATCCGCCCATCGTCACTGCCGTTAATCTGCGTGATACGATCACGAAGCGTATTCa                                                                                                                                      >  2:1316347/1‑150 (MQ=255)
                 ggAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTcc                                                                                                                                      >  2:1593672/1‑149 (MQ=255)
                   aaCACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGt                                                                                                                                    <  1:692460/150‑1 (MQ=255)
                          ccgccACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCGAGCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCg                                                                                                                             >  1:1238328/1‑150 (MQ=255)
                              caccaTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTccc                                                                                                                         <  1:1015369/150‑1 (MQ=255)
                              caccaTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGACGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTccc                                                                                                                         >  2:1051566/1‑150 (MQ=255)
                                ccaTCAACACGCAGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCCGCTGTCATCCATCCGCACTTCGTCAATGGCGTTAATCTGCGTGATACGATCCCTCAGCGTATTCAGTGATTCCGTCCCCt                                                                                                                       >  2:315950/1‑150 (MQ=255)
                                 caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                      <  1:1435370/150‑1 (MQ=255)
                                 caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                      <  2:1600454/150‑1 (MQ=255)
                                 caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGCGCAAACCCGCTGTCAGCCATCCGCACTTCGTCACTGCCGATAATCAGCGTGATACGATCACGCCGCGTATTCAGGGATTCCGTccccgg                                                                                                                      >  1:1886973/1‑148 (MQ=255)
                                   tCACCACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCCTCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGTTTCCGTCCCCTGGa                                                                                                                    >  2:153685/1‑150 (MQ=255)
                                     aaCACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGaaa                                                                                                                  <  1:598927/150‑1 (MQ=255)
                                     aaCACGCCGATCATCGCCAAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGaaa                                                                                                                  >  1:488271/1‑150 (MQ=255)
                                          gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCACCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCTGTGATTCCGTCCCCTGGAAATCGAg                                                                                                             >  1:580823/1‑150 (MQ=255)
                                          gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGTGCAAACGAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                             <  1:96922/150‑1 (MQ=255)
                                          gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                             <  1:1666990/150‑1 (MQ=255)
                                                 atcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCACACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                      <  2:892229/150‑1 (MQ=255)
                                                    gCCGAAACGCGCCCGACCAGCCCGGTCAGCGCCGCCAGCCGGGCACACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCCCATCACGCAGCGTATTCAGTGATTCCGTCCCCGGGAAATCGAGGTTCGGGATc                                                                                                   >  2:982065/1‑150 (MQ=255)
                                                    gCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCCGCGTATTCAGTGATTCCGTCCCCTGGAAGTCGAGTTTCGGGCTc                                                                                                   >  1:1053326/1‑150 (MQ=255)
                                                     ccGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTGCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATca                                                                                                  <  1:1820616/150‑1 (MQ=255)
                                                       gAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATcacc                                                                                                <  2:1426348/150‑1 (MQ=255)
                                                         aaCGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCTAACCAGCTGTCATCCATCCGCACTTCGTGAATGCCGTTAATCTGCGTGATACGAGCACGCAGCGTATTCAGTGATTCCGTCCCCTTGAAATCGAGTGTCGGGATcaccac                                                                                              >  2:135026/1‑150 (MQ=255)
                                                          aCGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCCCTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCtccacc                                                                                             >  2:1197865/1‑150 (MQ=255)
                                                               cccGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGGATTCAGTGAGTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCAc                                                                                        >  1:948200/1‑150 (MQ=255)
                                                                  gACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGc                                                                                     <  2:888354/150‑1 (MQ=255)
                                                                  gACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGc                                                                                     <  1:488088/150‑1 (MQ=255)
                                                                  gACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGATGTCATCCATCCGCACTTCGGCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGc                                                                                     <  2:954882/150‑1 (MQ=255)
                                                                     cAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCgg                                                                                  >  1:1457632/1‑150 (MQ=255)
                                                                      aGCCCGGTCAACGCCGCCAGAAGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGa                                                                                 <  1:1394945/150‑1 (MQ=255)
                                                                       gCCCGGTCAACGCCGCCAGACGGGCAAACGAGCTGTCATCAATGCGCTCTTCGTCATTGCCGTTAATCTGCGTGTTACGTTCACGCAGCGTATTCAGTGAGTCCGTCCCCTGGAATTAGAGTTTCGGGATCTCCACCGCCACTGCCGGat                                                                                >  2:87244/1‑149 (MQ=255)
                                                                         ccGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGc                                                                              >  2:1159239/1‑150 (MQ=255)
                                                                         ccGGTCAACGCCGCCAGACGGGAAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCATGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGc                                                                              <  2:1496318/150‑1 (MQ=255)
                                                                               aaCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGGTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGttt                                                                        <  2:589759/150‑1 (MQ=255)
                                                                                aCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtttt                                                                       <  1:1149817/150‑1 (MQ=255)
                                                                                     gccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGGGTGATACGATCACTCAGCGTATTCCGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCTCCACTGCTGGAAGCGGGTTTTCTTcc                                                                  <  2:747472/150‑1 (MQ=255)
                                                                                      ccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCa                                                                 <  1:803992/150‑1 (MQ=255)
                                                                                       cAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAg                                                                <  2:204006/150‑1 (MQ=255)
                                                                                        aGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                               >  2:884205/1‑150 (MQ=255)
                                                                                        aGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                               <  1:88720/150‑1 (MQ=255)
                                                                                         gACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGTGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCa                                                              >  1:842961/1‑150 (MQ=255)
                                                                                         gACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCa                                                              >  1:1832491/1‑150 (MQ=255)
                                                                                         gACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCa                                                              <  1:151032/150‑1 (MQ=255)
                                                                                           cGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata                                                            >  2:590504/1‑150 (MQ=255)
                                                                                              gCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCTCCACCGCCACTGCCCGAAGCGGGGTTTCTTCAAGCATATcc                                                         >  2:843266/1‑150 (MQ=255)
                                                                                               cAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCa                                                        >  1:785557/1‑150 (MQ=255)
                                                                                               cAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGAGATACGATCACGCAGCGTATTCAGTGATTCAGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCa                                                        >  1:1518494/1‑150 (MQ=255)
                                                                                                aaaCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAg                                                       <  2:1279984/150‑1 (MQ=255)
                                                                                                 aaCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGAAACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCCCTGCCGGAAGCGGGTTTTCTTCCAGCATATCCCgc                                                      >  1:200533/1‑150 (MQ=255)
                                                                                                   ccAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAgcgc                                                    <  1:143916/150‑1 (MQ=255)
                                                                                                    cAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGca                                                   >  2:1866101/1‑150 (MQ=255)
                                                                                                      gCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcacc                                                 >  2:1162437/1‑150 (MQ=255)
                                                                                                       cTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcacca                                                <  1:459985/150‑1 (MQ=255)
                                                                                                        tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                               <  2:52631/150‑1 (MQ=255)
                                                                                                         gTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCaccacc                                              >  1:281546/1‑150 (MQ=255)
                                                                                                          tCATACATCCGCACTTCGTCAATGCAGTTAATCTGCGTGATACGATCACGAAGCGTAATCAGAGACTCCTTCCCCTCGAAATCGAGATTCGGCATCACCACCGCCACAGCCGGAAGCTGGTTTTCTTCCAGCATATCCAGCGCAccacca                                             <  1:315950/150‑1 (MQ=255)
                                                                                                            atccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaaa                                           >  1:1983591/1‑150 (MQ=255)
                                                                                                              ccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCTGGTTTTCTTCCAGCATATCCAGCGCACCACCACAac                                         >  2:487400/1‑150 (MQ=255)
                                                                                                              ccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAac                                         <  1:1700618/150‑1 (MQ=255)
                                                                                                                 tccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccag                                      >  2:736926/1‑150 (MQ=255)
                                                                                                                   cGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagac                                    <  1:1269215/150‑1 (MQ=255)
                                                                                                                   cGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagac                                    >  1:1020008/1‑150 (MQ=255)
                                                                                                                   cGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagac                                    >  1:549317/1‑150 (MQ=255)
                                                                                                                   cGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagac                                    <  1:495841/150‑1 (MQ=255)
                                                                                                                     cACTACGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTAATCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacaa                                  >  1:1870233/1‑148 (MQ=255)
                                                                                                                      aCTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCCCCGCCACTGCCGGAAGCGGGTGTGCTTCCAGCATATCCAGCGCACCACCAACaccagaccag                                 >  2:119616/1‑150 (MQ=255)
                                                                                                                          cGTCAATGCCCTTAATCTGCGTGATACGATCACGCAGCGTAATCAGTGATTCCGTCCCCTGGAAATCGAGTATCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTAc                             <  1:1802034/150‑1 (MQ=255)
                                                                                                                           gTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACg                            >  1:987296/1‑150 (MQ=255)
                                                                                                                            tCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACgg                           <  2:1852480/150‑1 (MQ=255)
                                                                                                                            tCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACgg                           <  1:713913/150‑1 (MQ=255)
                                                                                                                             cAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGa                          >  2:591861/1‑150 (MQ=255)
                                                                                                                               aTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAAc                        >  1:1615092/1‑150 (MQ=255)
                                                                                                                                 gCCGTTAATCTGCGTGATACGAGCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTc                      <  1:1118649/150‑1 (MQ=255)
                                                                                                                                  ccGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTcc                     >  1:825032/1‑149 (MQ=255)
                                                                                                                                      tacgcTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCa                 >  2:1664864/5‑150 (MQ=255)
                                                                                                                                      tAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCa                 <  1:1297666/150‑1 (MQ=255)
                                                                                                                                       aaTCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGGTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTGTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAg                <  1:1197865/150‑1 (MQ=255)
                                                                                                                                        cgcTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGAAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGt               >  2:1829011/3‑150 (MQ=255)
                                                                                                                                         tCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTg              <  1:1643739/150‑1 (MQ=255)
                                                                                                                                         gcTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCCACGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTg              >  1:377660/2‑150 (MQ=255)
                                                                                                                                         gcTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTg              >  2:896301/2‑150 (MQ=255)
                                                                                                                                           tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACGACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCg            <  2:1777090/150‑1 (MQ=255)
                                                                                                                                           tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAACACCAGCCCAGTTACGGAACTCACCCAGTGCg            >  1:1697658/1‑150 (MQ=255)
                                                                                                                                             cgagATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTGTCGGGATCACCCCCGCCACTGACGGAGGCGGGTTTACTTCCAGCATATCAAGCGCGCCACAAAACCGAGACCAGTTTCGGAACGCAGCCACTGCGTc          >  2:73822/4‑150 (MQ=255)
                                                                                                                                                gATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGCTCCCCACCGCCACTGCCGCAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAACCCAGCCACGGTACGGATCTGACCCAGTGCGTCTTc       >  1:370918/1‑150 (MQ=255)
                                                                                                                                                gATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGCACTCACCCAGTGCGTCTTc       >  2:717621/1‑150 (MQ=255)
                                                                                                                                                  tACGATCACACAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCAc     <  1:1945646/150‑1 (MQ=255)
                                                                                                                                                    cGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACga   >  2:869039/1‑150 (MQ=255)
                                                                                                                                                     gATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGATTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGACTTCACgag  >  1:279789/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAG  >  NC_000913/3601358‑3601656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: