Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,648,132 A→C 5.6% noncoding (70/84 nt) agrA → inactive antisense sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,648,1320AC 5.6% 373.7 / 10.4 126noncoding (70/84 nt)agrAinactive antisense sRNA
Reads supporting (aligned to +/- strand):  ref base A (40/79);  new base C (4/3);  total (44/82)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

Only 100 of 126 total aligned reads displayed.

GGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647984‑3648278
                                                                                                                                                    |                                                                                                                                                  
ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAg                                                                                                                                                   >  2:1092837/1‑150 (MQ=255)
ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTAAGGGCTGGAAAGTAAAAAACCGACGCAAAg                                                                                                                                                   >  2:1583663/1‑150 (MQ=255)
   tAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGATGCAAAGTCg                                                                                                                                                >  2:942795/1‑150 (MQ=255)
     aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCATTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                                                                                                              >  1:1809769/1‑150 (MQ=255)
     aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCTGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACGAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                                                                                                              >  1:106977/1‑150 (MQ=255)
     aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                                                                                                              <  2:1224316/150‑1 (MQ=255)
     aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                                                                                                              >  1:460571/1‑150 (MQ=255)
       cATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGTCTATCTCACTCCAGTCAGAGACAACAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAACTCCGttt                                                                                                                                            >  1:328751/1‑150 (MQ=38)
         tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttt                                                                                                                                          <  2:1305616/150‑1 (MQ=255)
          aCAGTACATGCAGTTTATAAAACCAGCACGTCCTTGCTATAGTTTCAGTATGGTATTTTCATTTATGCGTTAGATGATGGCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  1:649041/150‑1 (MQ=37)
          aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCATCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  1:1998673/150‑1 (MQ=255)
          aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                         <  2:848855/150‑1 (MQ=38)
          aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  2:220883/150‑1 (MQ=255)
           cAGTACATTCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  2:1298543/150‑1 (MQ=255)
           cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  2:1851871/150‑1 (MQ=255)
           cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  1:337272/150‑1 (MQ=255)
            actACCTGCATGCTCTAAAACTAGCAACTTCTTGCAATTGTTTCATTTTGGTATTAGAATTGATGCGTTAGATTATTTCTATCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                       <  2:1368706/148‑1 (MQ=17)
            aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                       <  2:1381902/150‑3 (MQ=38)
            aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                       <  1:472188/150‑1 (MQ=255)
            aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                       >  1:2000296/1‑150 (MQ=255)
            aGTACATGCAGGTTATAAAACCAGCACGGCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                       <  1:694181/150‑1 (MQ=255)
            aGTACATGCAGGGTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                       <  1:1088202/150‑3 (MQ=38)
             gTACATGCAGGTTAAAACACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTATATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                      <  2:953303/150‑1 (MQ=255)
                  tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                 >  1:149460/1‑150 (MQ=255)
                        ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                           <  2:1219073/150‑1 (MQ=255)
                        ttATAAAACCAGCACGTCCTGGCAATAGTTGCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCCATCTCACTCCAGTCAGAGCCACCCACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                           <  1:1500684/150‑1 (MQ=255)
                         tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCACCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGGTTTTTTACATCAGGATTCgg                                                                                                                          >  1:1110210/1‑150 (MQ=255)
                         tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCgg                                                                                                                          <  2:404136/150‑1 (MQ=255)
                         tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCgg                                                                                                                          >  2:574784/1‑150 (MQ=255)
                          ataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCAACCAACTGCGGGCTGGAAAGTAAAAAACAGCCGCAAAGTCGGTTTGTTTACAGACGGATACggc                                                                                                                         >  1:1388031/1‑149 (MQ=255)
                           tataaacAGCAATTCCTTGCAATTTTTTCATTTTGGTATTAGCATTGATGTGTTAGATTATTTCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAc                                                                                                                        <  2:1080420/144‑1 (MQ=14)
                           taAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGAc                                                                                                                        <  1:193138/150‑1 (MQ=255)
                            aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGACGGTTTTTTTACATCCGGATTCGGACa                                                                                                                       >  2:323676/1‑150 (MQ=255)
                              aaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAg                                                                                                                     <  2:1815606/150‑1 (MQ=255)
                               aCCAGCACGTCCTTGAAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCATGGATGGAAAGTAAAAAACAGACGAAAAGTCGGTTTTTTTACATCAGGATTCTGACATgg                                                                                                                    >  1:1894796/1‑150 (MQ=255)
                                  aGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCtt                                                                                                                 >  2:544960/1‑150 (MQ=255)
                                   gCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGTGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGTCTTa                                                                                                                >  1:1210004/1‑150 (MQ=255)
                                   gCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTa                                                                                                                >  2:951980/1‑150 (MQ=255)
                                     aCGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGTGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAat                                                                                                              <  1:627567/150‑1 (MQ=255)
                                     aCGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATTATGGCTATCTAACTCCAGTCAGAGACACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAat                                                                                                              <  2:1272560/150‑1 (MQ=255)
                                        tCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCTACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATg                                                                                                           >  1:686235/1‑150 (MQ=255)
                                            tGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGa                                                                                                       <  2:1485604/150‑1 (MQ=255)
                                             gCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGTAAAAGGCTTAATATGACGAt                                                                                                      >  1:362838/1‑150 (MQ=255)
                                                     ttCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGt                                                                                              <  1:1340743/150‑1 (MQ=255)
                                                         gTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCTGATTCTGACATGGATTAATATGACGATGAACCTGTGaaa                                                                                          >  1:1946977/1‑150 (MQ=25)
                                                          tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                         <  2:1931491/150‑1 (MQ=255)
                                                            tGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGta                                                                                       <  1:1281911/150‑1 (MQ=255)
                                                             ggTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtat                                                                                      >  1:660585/1‑150 (MQ=255)
                                                               tATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatat                                                                                    <  2:1399563/150‑1 (MQ=255)
                                                                    gCATTGATGCGGTAGATGATGTCTATCTCACTCCAGTAAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAGTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATc                                                                               <  1:1363687/150‑1 (MQ=255)
                                                                       ttGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGACACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTc                                                                            <  1:1476336/150‑1 (MQ=255)
                                                                            gCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGc                                                                       >  2:289993/1‑150 (MQ=255)
                                                                              gTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGa                                                                     <  1:1490960/150‑1 (MQ=255)
                                                                               ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGATGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                    <  1:942795/150‑1 (MQ=255)
                                                                               ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                    <  2:1961286/150‑1 (MQ=255)
                                                                                tAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGata                                                                   <  1:1526169/150‑1 (MQ=255)
                                                                                 aGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGatat                                                                  >  2:1377443/1‑150 (MQ=255)
                                                                                    tgatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGAtatata                                                               >  1:861415/1‑150 (MQ=255)
                                                                                     gatgGCTAGCTCACTCCATGCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGTATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAc                                                              <  1:1143238/150‑1 (MQ=255)
                                                                                      ttgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                             <  2:765845/149‑1 (MQ=255)
                                                                                      atgGCTATCTCTCTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                             <  1:439829/150‑1 (MQ=255)
                                                                                      atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                             <  2:501795/150‑1 (MQ=255)
                                                                                       tgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCg                                                            <  2:564620/150‑1 (MQ=255)
                                                                                        ggCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGa                                                           <  1:1086944/150‑1 (MQ=255)
                                                                                          cTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                         <  1:408832/150‑1 (MQ=255)
                                                                                            aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAATTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                       <  1:1919155/150‑1 (MQ=255)
                                                                                               tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                    <  2:445984/150‑1 (MQ=255)
                                                                                                 aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTATAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccc                                                  <  2:1723104/150‑1 (MQ=255)
                                                                                                  cTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCa                                                                           >  1:128815/1‑124 (MQ=255)
                                                                                                  cTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCa                                                                           <  2:128815/124‑1 (MQ=255)
                                                                                                    acAGTCAGATCCACCAACTCAGGGCTGGAAAGTAAAATACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccctcc                                               <  1:168361/149‑1 (MQ=255)
                                                                                                     cAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                              <  2:544866/150‑1 (MQ=255)
                                                                                                      aGTCAGAGCCACCAACTAATGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATTCCGAAGTGCTCCCTCCGc                                             <  2:1500323/150‑1 (MQ=255)
                                                                                                        taagagCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCa                                           <  1:251028/148‑1 (MQ=255)
                                                                                                               caccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                    <  2:1809769/150‑1 (MQ=255)
                                                                                                               caccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                    <  1:1880038/150‑1 (MQ=255)
                                                                                                               caccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                    <  2:1142784/150‑1 (MQ=255)
                                                                                                                    aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATc                               >  1:1690835/1‑150 (MQ=255)
                                                                                                                    aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATc                               <  1:289993/150‑1 (MQ=255)
                                                                                                                    aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGACTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCTCCAGCTGAAGAAATc                               >  2:1467225/1‑150 (MQ=255)
                                                                                                                     cTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                              <  2:350800/150‑1 (MQ=255)
                                                                                                                     atCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                              <  1:1643942/149‑1 (MQ=255)
                                                                                                                      tCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCTGATTCTGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                             <  1:323676/150‑1 (MQ=255)
                                                                                                                       cAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                            <  1:1747850/150‑1 (MQ=255)
                                                                                                                         gggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTaa                          >  1:688819/1‑150 (MQ=255)
                                                                                                                         gggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTaa                          >  1:201002/1‑150 (MQ=255)
                                                                                                                          ggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAt                         <  1:951980/150‑1 (MQ=255)
                                                                                                                                atgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                   >  1:397109/6‑150 (MQ=255)
                                                                                                                                aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                   <  1:1385157/150‑1 (MQ=255)
                                                                                                                                   ttAAAAAACCGACTCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGAGATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                <  1:1448624/149‑1 (MQ=255)
                                                                                                                                   taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                >  2:1507835/3‑150 (MQ=255)
                                                                                                                                   taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATACGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAAACGTCACTGCGATATATACCGAAGTGCTCAATCCGCCAGATGAAGAAATCGCTAATTCTTGAAAt                >  1:1879801/3‑150 (MQ=255)
                                                                                                                                     aaaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAATGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGt              >  2:1612441/1‑150 (MQ=255)
                                                                                                                                       aaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTa            >  1:452054/1‑150 (MQ=255)
                                                                                                                                        aaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAAGCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAg           <  2:1896400/150‑1 (MQ=255)
                                                                                                                                           ccGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTACCTCCGCCAGCTGAAGAAATCGCTAATTCTTGAAATGTTAGCCa        >  2:1148312/1‑150 (MQ=255)
                                                                                                                                             gACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt      >  2:1533942/1‑150 (MQ=255)
                                                                                                                                               cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg    <  1:1152507/150‑1 (MQ=255)
                                                                                                                                                gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGc   <  1:544960/150‑1 (MQ=255)
                                                                                                                                                 cAAAGTCGGTTTTTTTACATCCGGATTCGGTCAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCTTCACTGCGATATATACCTAAGTGCTCCCTCCGCCAGCTGTAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt  <  1:1025695/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647984‑3648278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑