Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,663,564 G→A 5.3% intergenic (+37/+326) mdtF → / ← gadW anaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,663,5640GA 5.3% 416.8 / 5.3 130intergenic (+37/+326)mdtF/gadWanaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (63/60);  new base A (3/4);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTAATC  >  NC_000913/3663415‑3663713
                                                                                                                                                     |                                                                                                                                                     
  ggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                                     >  1:739913/1‑150 (MQ=255)
  ggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                                     <  1:27868/150‑1 (MQ=255)
  ggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                                     <  2:62016/150‑1 (MQ=255)
      ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGGCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTAc                                                                                                                                                 <  1:1543405/150‑1 (MQ=255)
       cGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTAct                                                                                                                                                <  1:74221/150‑1 (MQ=255)
       cGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTAct                                                                                                                                                <  1:211554/150‑1 (MQ=255)
        ggggTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActc                                                                                                                                               <  2:1836466/147‑1 (MQ=255)
         gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGTAGGTTCAGCCTTActct                                                                                                                                              >  1:139861/1‑150 (MQ=255)
         gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActct                                                                                                                                              <  2:1305916/150‑1 (MQ=39)
            ggATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACAGCTCTTTGCCCGCTTTAAAAAAGCGTAACGGGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTc                                                                                                                                           <  1:747255/149‑1 (MQ=255)
             taTGGGCGGGATGTTTGCAGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTTGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTcc                                                                                                                                          <  2:433626/149‑1 (MQ=255)
                 ggCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCt                                                                                                                                      >  1:1330214/1‑150 (MQ=255)
                  gCGGGATGTTTGCCGCAACAGGGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTATGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCtc                                                                                                                                     <  2:257055/150‑2 (MQ=255)
                   cGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTaa                                                                                                                                    >  1:838594/1‑150 (MQ=255)
                         gTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGTGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTc                                                                                                                              >  2:1503363/1‑150 (MQ=255)
                          tttGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTca                                                                                                                             <  1:1031565/150‑1 (MQ=255)
                             gCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacca                                                                                                                          >  1:1076612/1‑150 (MQ=255)
                              ccGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcaccac                                                                                                                         >  1:493372/1‑150 (MQ=255)
                               cGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACt                                                                                                                        >  1:231780/1‑150 (MQ=255)
                                gCAACAGTGCTGGCAATTTACTTCGTTCCGGGCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTc                                                                                                                       <  1:178139/150‑1 (MQ=255)
                                  aaCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCATAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaa                                                                                                                     <  1:1621962/150‑1 (MQ=255)
                                    cAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaaca                                                                                                                   >  2:79878/1‑150 (MQ=255)
                                    cAGTGCGGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAATCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaaca                                                                                                                   <  1:2018444/150‑1 (MQ=255)
                                      gTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCaacaac                                                                                                                 >  1:1785341/1‑150 (MQ=255)
                                          tGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGc                                                                                                             >  1:761121/1‑150 (MQ=255)
                                          tGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGc                                                                                                             <  2:1379651/150‑1 (MQ=255)
                                           ggCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCt                                                                                                            >  2:1528576/1‑150 (MQ=255)
                                           ggCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCt                                                                                                            <  1:531052/150‑1 (MQ=255)
                                            gCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGTACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTa                                                                                                           >  2:136677/1‑150 (MQ=255)
                                             cAAGTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGATAACCATTCACCACTCAGCACCCAGCTaa                                                                                                          >  2:303474/1‑150 (MQ=255)
                                                  tACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTc                                                                                                     <  2:640150/150‑1 (MQ=255)
                                                  tACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTc                                                                                                     >  1:1700984/1‑150 (MQ=255)
                                                    cTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAg                                                                                                   >  1:1177466/1‑150 (MQ=255)
                                                     ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAgg                                                                                                  <  1:1180215/150‑1 (MQ=255)
                                                     ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAgg                                                                                                  <  2:1662147/150‑1 (MQ=255)
                                                       ggTTCCGGTCTTTTTCGTTGTAGTGTAACATCTCTTTGCCCGCTTTAAAAAAGCGTCACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCAGCACTCAACAACCAGCTAACCGTCAGGaa                                                                                                <  2:1574063/149‑1 (MQ=255)
                                                         ttCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATa                                                                                              >  1:838553/1‑150 (MQ=255)
                                                         ttCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATa                                                                                              >  1:107257/1‑150 (MQ=255)
                                                            cGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCa                                                                                           <  1:894780/150‑1 (MQ=255)
                                                             ggTGTTTTTCGTTGTAGTGGATCATCTCTTTGCCCGCTTTAAAAAAGCGTTACGTGTAAATGAGAGTAATGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCTTTCACCACTCATCAACCAGCTAACCGTCAGGAATAcctg                                                                                          <  1:1223424/150‑3 (MQ=255)
                                                               tCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGc                                                                                        <  2:1303565/150‑1 (MQ=255)
                                                                cTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCt                                                                                       >  2:712984/1‑150 (MQ=255)
                                                                 tttttCTTTGTAGTGTAAAATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCtt                                                                                      <  1:999155/150‑1 (MQ=255)
                                                                   tttCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAg                                                                                    <  1:1674675/150‑1 (MQ=255)
                                                                     tCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTa                                                                                  >  2:602631/1‑150 (MQ=255)
                                                                     tCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTa                                                                                  <  2:1240858/150‑1 (MQ=255)
                                                                     tCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTa                                                                                  <  1:237633/150‑1 (MQ=255)
                                                                      cGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCCGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTac                                                                                 <  1:1483686/150‑1 (MQ=255)
                                                                       gTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTaca                                                                                <  1:948929/150‑1 (MQ=255)
                                                                        ttGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACGAGCTAACCGTCAGGAATACCAGGCTTAGTacac                                                                               <  2:1042177/150‑1 (MQ=255)
                                                                           tAGTGGAACATCTCTTTTCCAGCTTTAAAAAAGCGTAACGTGTAAATGATAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGc                                                                            <  2:1650063/150‑1 (MQ=255)
                                                                           tAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACATCCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGc                                                                            >  1:1707084/1‑150 (MQ=255)
                                                                             gTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCAc                                                                          >  1:1437666/1‑150 (MQ=255)
                                                                                 aaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGaa                                                                      >  1:850678/1‑150 (MQ=255)
                                                                                 aaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGaa                                                                      <  2:735014/150‑1 (MQ=255)
                                                                                 aaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGaa                                                                      <  1:735013/150‑1 (MQ=255)
                                                                                   cATCTCTTTGCACGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAg                                                                    >  2:316310/1‑150 (MQ=255)
                                                                                     tctcTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTa                                                                  >  1:1536085/1‑150 (MQ=255)
                                                                                      ctctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAg                                                                 >  2:250503/1‑150 (MQ=255)
                                                                                       tctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGa                                                                <  2:1890681/150‑1 (MQ=255)
                                                                                        ctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGaa                                                               <  1:448043/150‑1 (MQ=255)
                                                                                        ctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTAAGTACACAGCACTGAAAGTAGaa                                                               >  2:825143/1‑150 (MQ=255)
                                                                                          ttGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTAAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACCGCACTGAAAGTAGAAAc                                                             >  2:538420/1‑150 (MQ=255)
                                                                                             cccGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCAc                                                          >  1:362221/1‑150 (MQ=255)
                                                                                               cGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTACCCGTCAGGAATACCAGGCTTAGTACACAGCACTCAAAGTAGAACCCCCTc                                                        >  1:449772/1‑150 (MQ=255)
                                                                                                    tAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACATCACTGAAAGTAGAAACCACTCCAGcc                                                   >  2:635984/1‑150 (MQ=255)
                                                                                                      aaaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCAt                                                 >  1:1881542/1‑150 (MQ=255)
                                                                                                      aaaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCATGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCAt                                                 >  2:43181/1‑150 (MQ=255)
                                                                                                      aaaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTAACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGACAGGAATACCAGGCTAAGTACACCGCACTGAAAGTAGAACCCACTCCAGCCAt                                                 >  1:1421644/1‑150 (MQ=255)
                                                                                                         aaGCGTAACGTGTAACTGAGAGTAAGGTTGAACATGAATGTTCAGACTTACTCTTTCCTGCTAAACATTAACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTAGACATCACTGAAAGTAGAAACCACTCCAGCCATTCg                                              <  2:78543/150‑1 (MQ=255)
                                                                                                           gCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtc                                            <  2:1317206/150‑1 (MQ=255)
                                                                                                            cGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtca                                           >  2:1948052/1‑150 (MQ=255)
                                                                                                             gTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcat                                          <  1:1219159/150‑1 (MQ=255)
                                                                                                                aCGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCaa                                       <  1:904513/150‑1 (MQ=255)
                                                                                                                 cGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAAt                                      >  1:1008996/1‑150 (MQ=255)
                                                                                                                    gtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTg                                   <  2:867935/150‑1 (MQ=255)
                                                                                                                      aggaaagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCCGCTAACCGTCAGGAATACCAGGATAAGTACACAGCACGCAAACTAGAAACCCCTCCAGCCATTCGTCATCAATTTGaa                                 >  1:195153/6‑150 (MQ=25)
                                                                                                                      aaaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaa                                 >  1:63041/1‑150 (MQ=255)
                                                                                                                      aaaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaa                                 >  1:781078/1‑150 (MQ=255)
                                                                                                                      aaaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaa                                 >  1:1194566/1‑150 (MQ=255)
                                                                                                                       aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaac                                <  2:600599/150‑1 (MQ=255)
                                                                                                                       aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaac                                <  1:68835/150‑1 (MQ=255)
                                                                                                                        aTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaaca                               >  2:1654254/1‑150 (MQ=255)
                                                                                                                         tGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaacaa                              >  2:1932522/1‑150 (MQ=255)
                                                                                                                            cagTAACGTTTAACATGAAGGTTCAGCCTTACTATTTCCTGATAACAATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCATGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAacac                           <  1:83191/149‑1 (MQ=255)
                                                                                                                                     tGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCAATTCGTCATCAATTTGAACAACACAATACTGAc                  >  1:963777/1‑150 (MQ=255)
                                                                                                                                      taaCATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAcc                 <  1:1643790/149‑1 (MQ=255)
                                                                                                                                      gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAcc                 <  2:231780/150‑1 (MQ=255)
                                                                                                                                      gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCAATCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAcc                 >  1:1894411/1‑150 (MQ=255)
                                                                                                                                       aaCATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACCCAATACTGAccc                >  2:1464520/1‑150 (MQ=255)
                                                                                                                                        aCATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGCCCca               >  2:1885489/1‑150 (MQ=255)
                                                                                                                                         cATGAAGGTTCAGCCTTACTCTTTCCTGCTACCCATTCACCCCTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGAAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCcac              >  2:1265292/1‑150 (MQ=255)
                                                                                                                                         cATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCcac              >  2:1842898/1‑150 (MQ=255)
                                                                                                                                         cATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAACTATCAAACACTCCAGCCATTCGTCATCAATTTGAACAACACCATACTGcaccac              >  2:372042/1‑150 (MQ=255)
                                                                                                                                            gAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCCCTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACAtt           >  2:1854580/1‑150 (MQ=255)
                                                                                                                                            gAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCAAGCCATTCTTCATCAATTTGAACAACACAAAACTGCACCACAtt           >  2:109143/1‑150 (MQ=255)
                                                                                                                                             aaGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTc          <  1:1228939/150‑1 (MQ=255)
                                                                                                                                              aGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTcc         <  2:868609/150‑1 (MQ=255)
                                                                                                                                                gTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCg       >  1:403481/1‑150 (MQ=255)
                                                                                                                                                     gCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTAATc  <  1:1912189/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTAATC  >  NC_000913/3663415‑3663713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: