Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,701,840 A→G 6.3% intergenic (+6/+24) yhjV → / ← dppF putative transporter/dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,701,8400AG 6.3% 365.8 / 11.7 128intergenic (+6/+24)yhjV/dppFputative transporter/dipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base A (62/55);  new base G (2/6);  total (66/62)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

Only 100 of 129 total aligned reads displayed.

TATGGTATTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAG  >  NC_000913/3701691‑3701987
                                                                                                                                                     |                                                                                                                                                    
tatGGTATTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGa                                                                                                                                                      <  1:687073/150‑1 (MQ=255)
 atGGTATTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAg                                                                                                                                                     <  1:1740943/150‑1 (MQ=255)
      aTTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCAt                                                                                                                                                >  2:1898956/1‑150 (MQ=255)
             cGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCACCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCg                                                                                                                                        <  2:150510/150‑1 (MQ=255)
                gCCTCATTCCGTTTTTCCTGATCTATAACGGCGCACAACCGGAAAACCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCGGATTGAGTAAACCGGAGCGCATGGCCCCGGtt                                                                                                                                     <  2:1237179/150‑1 (MQ=255)
                gCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCACGGCCCCGGtt                                                                                                                                     >  1:1355734/1‑150 (MQ=255)
                  cTCATTCCGTTTTTCCTGAGCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                   <  1:1821350/150‑1 (MQ=255)
                   tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                  <  2:623862/150‑1 (MQ=255)
                   tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                  <  1:870825/150‑1 (MQ=255)
                   tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTGCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                  <  1:895398/150‑1 (MQ=255)
                    cATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttact                                                                                                                                 <  1:1697966/150‑4 (MQ=255)
                    cATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                 <  1:903489/150‑1 (MQ=255)
                    cATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATGTAAAGCCTGGCTGACTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttact                                                                                                                                 <  1:1370905/150‑4 (MQ=255)
                     aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAACCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactc                                                                                                                                <  1:563675/150‑5 (MQ=255)
                     aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgtg                                                                                                                                <  2:1896336/150‑1 (MQ=255)
                     aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCAAGTTGCTTTGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactc                                                                                                                                <  1:1162513/150‑5 (MQ=255)
                     aTTCCGTTTTACCTGATCTATAAAGTCGCACAACTGCAAAAACTTCGCGGATTTAAAGCCTGGCGGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCAGGGCCCCCGTTTtgtg                                                                                                                                <  1:1405602/150‑1 (MQ=255)
                         cGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGtt                                                                                                                            >  1:1069398/1‑150 (MQ=255)
                             tttCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACg                                                                                                                        >  2:593979/1‑150 (MQ=255)
                               tCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCt                                                                                                                      >  1:27570/1‑150 (MQ=255)
                                 cTGATCTATAAAGTCGCTCAACTGGAAAAACTTGGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACAGGAGCGCATCGCCCCGGTTTTGTGACTTAAAGCTGc                                                                                                                    >  2:1533452/1‑150 (MQ=255)
                                 cTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTAAGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                                                    >  2:1955385/1‑150 (MQ=255)
                                  tGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCGGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCg                                                                                                                   <  1:1205988/150‑1 (MQ=255)
                                   gATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCgg                                                                                                                  >  2:455671/1‑150 (MQ=255)
                                       tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                              <  1:1803579/150‑1 (MQ=255)
                                          aaaGTCGCACAACTCGAAAAACTTCGCGGATTTAAAGCCTGGCTGAATCAGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGAATGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                                           <  1:978097/150‑1 (MQ=255)
                                           aaGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGACTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTACCGCTGCGGATTTTCAt                                                                                                          >  1:1969203/1‑150 (MQ=255)
                                             gTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTTAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATcc                                                                                                        <  1:1624420/150‑1 (MQ=255)
                                                gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                     >  1:1200749/1‑150 (MQ=255)
                                                gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                     >  2:1051912/1‑150 (MQ=255)
                                                gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGACCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                     >  1:1568759/1‑150 (MQ=255)
                                                  ccaACTGGAAAAACTTCGCGGAATGATCGCCGCGCTGCTTCTGCTGGACGGCAGATTGCTAGCCTTGTCGCCACTGTGGCAGCTGCTAGAGTAATCCGGAGCGCTTGGTCCCGGTTTTGTGAGTGCCCGCGGCGGATTTTCATCCTGATc                                                                                                   <  1:304362/149‑1 (MQ=255)
                                                  acaACTTGAAAAACTTCGCGGATGTAAAGCCTGGCGGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                                                   <  2:1277253/150‑1 (MQ=255)
                                                  acaACTGGAAAAACTTCGCGGAGTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGACGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                                                   <  2:403699/150‑1 (MQ=255)
                                                   caACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGCGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCa                                                                                                  >  2:1235393/1‑150 (MQ=255)
                                                   caACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCa                                                                                                  <  2:836795/150‑1 (MQ=255)
                                                        ggAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGc                                                                                             >  1:404021/1‑150 (MQ=255)
                                                                  ggatgATTTAAAGCCTGTCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                                                   <  2:1701792/146‑1 (MQ=255)
                                                                         ttAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTT                                                                            >  2:1006039‑M1/1‑150 (MQ=255)
                                                                           aaaGCCTGTCTGATTCTGCTGTACGGCATTTTGCTATGCTGGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCACGGTTTTGTGATTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                                          <  1:576830/150‑1 (MQ=255)
                                                                           aaaGCCTGGCTGCTTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                                          <  1:568865/150‑1 (MQ=255)
                                                                            aaGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTCCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAc                                                                         <  2:197015/150‑1 (MQ=255)
                                                                              gCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccg                                                                       >  2:1505081/1‑150 (MQ=255)
                                                                               ccTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccgc                                                                      <  1:191221/150‑1 (MQ=255)
                                                                                cTGGCTGATTATTCTGTACGGCATTTTGATATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAAACGGAGCGCATTGCCCCGTTTTTGTGAGTTAACGCTTCGGATTTTCATCCTGATCAACAGCAAAACTAGCTACCAGTTGAccgcc                                                                     <  1:304038/150‑1 (MQ=255)
                                                                                  ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGt                                                                   >  1:1427369/1‑150 (MQ=255)
                                                                                  ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCAGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGt                                                                   <  2:1007794/150‑1 (MQ=255)
                                                                                     tGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGt                                                                >  2:1009082/1‑150 (MQ=255)
                                                                                         tCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCTCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCttt                                                            <  2:188057/150‑1 (MQ=255)
                                                                                          ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                                           <  1:1663517/150‑1 (MQ=255)
                                                                                           tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGTCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                                          <  1:1020328/150‑1 (MQ=255)
                                                                                            gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                                         >  2:684392/1‑150 (MQ=255)
                                                                                             ctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGc                                                        <  2:1329746/150‑1 (MQ=255)
                                                                                               gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTg                                                      >  2:27890/1‑150 (MQ=255)
                                                                                                 aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                    >  1:1191463/1‑150 (MQ=255)
                                                                                                 aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                    >  2:220711/1‑150 (MQ=255)
                                                                                                 aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                    >  2:1321237/1‑150 (MQ=255)
                                                                                                  cGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGCCCGCCGTAGTCTTTTAGCTGCgg                                                   >  1:383812/1‑150 (MQ=255)
                                                                                                     cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGaccgccgcagctaaaagactacggcgg                                                <  2:1163836‑M1/150‑28 (MQ=255)
                                                                                                     cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGaccgccgcagctaaaagactacggcgg                                                <  1:761967‑M1/150‑28 (MQ=255)
                                                                                                     cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                                                <  1:858799/150‑1 (MQ=255)
                                                                                                     cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                                                <  2:1768376/150‑1 (MQ=255)
                                                                                                      aTTTTTCAATGCTTTTCGCCACTGTTGAAGCTGATTGTGTAACCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACTCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                               >  2:374478‑M2/1‑149 (MQ=255)
                                                                                                      aTTTTGCTATGCTTGTCGCCACTGTTGACGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                               >  1:167201‑M2/1‑149 (MQ=255)
                                                                                                      aTTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                               <  2:791139‑M2/150‑2 (MQ=255)
                                                                                                      aTTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                               <  2:18826‑M2/150‑2 (MQ=255)
                                                                                                         ttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaac                                            >  1:1515624‑M2/1‑146 (MQ=255)
                                                                                                            cTATGCTTGTCGCCACTGTTGAAGCTGATTTAGTAAACCGGAGCGCATTGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCATGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaactcg                                         <  1:266974‑M2/150‑8 (MQ=255)
                                                                                                            cTATGCTTGTCGCCACTGGTGTAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaactgg                                         >  2:356496‑M2/1‑143 (MQ=255)
                                                                                                             tATGCTTGGCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                                        <  2:85290/150‑1 (MQ=255)
                                                                                                               tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                      >  2:1157822/1‑150 (MQ=255)
                                                                                                               tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                      >  1:896548/1‑150 (MQ=255)
                                                                                                               tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                      >  1:759110/1‑150 (MQ=255)
                                                                                                               tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                      <  2:1974720/150‑1 (MQ=255)
                                                                                                                gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGc                                     >  1:1642769/1‑150 (MQ=255)
                                                                                                                gCTTGTCGCCACTGTTGAAGCTGAATGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGcagccgcagctaaaagactacggcggtcaactggtagc                                     <  1:681589‑M1/150‑39 (MQ=255)
                                                                                                                      cGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCGGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGc                               <  1:956700/150‑1 (MQ=255)
                                                                                                                        ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCg                             >  1:1194457/1‑150 (MQ=255)
                                                                                                                             gTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgcg                        >  2:414407/1‑150 (MQ=255)
                                                                                                                                  aGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgac                   >  2:948003/1‑150 (MQ=255)
                                                                                                                                  aGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgac                   <  1:1505081/150‑1 (MQ=255)
                                                                                                                                  aGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGAAGGGGCCGAAGCGCcgac                   >  1:848351/1‑150 (MQ=255)
                                                                                                                                   gCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgacg                  >  1:1598483/1‑150 (MQ=255)
                                                                                                                                    cTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgacga                 >  1:1835644/1‑150 (MQ=255)
                                                                                                                                     tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCgacgac                >  1:413494/1‑150 (MQ=255)
                                                                                                                                      gATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCCACTGGGTGCAGGGGCCGAAGCGCCgacgacc               >  1:778581/1‑149 (MQ=255)
                                                                                                                                      gATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACa               >  2:909872/1‑150 (MQ=255)
                                                                                                                                        ttGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGATGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGc             >  2:1436830/1‑150 (MQ=255)
                                                                                                                                         tGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCg            >  1:1949407/1‑150 (MQ=255)
                                                                                                                                          cacaaaaCCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCgg           >  2:1269897/5‑150 (MQ=255)
                                                                                                                                            caaaaCCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTTGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGc         >  1:1798814/3‑150 (MQ=255)
                                                                                                                                             tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGCAGCGCCGCCGACAGCGGGCg        >  2:1489296/1‑150 (MQ=255)
                                                                                                                                             tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCg        >  1:816063/1‑150 (MQ=255)
                                                                                                                                             tAAACCGGAGCGCACGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCg        <  2:1355734/150‑1 (MQ=255)
                                                                                                                                              aaaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGGAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCgg       >  2:727248/1‑149 (MQ=255)
                                                                                                                                               aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGCCCGCCGTATTCTTTTAGCTGCGGCAGCAACTGGGTGAAGGGGGCGAATCGCCGACGACAGCGGACgat      >  2:152221/1‑148 (MQ=255)
                                                                                                                                               aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGtt      <  1:782589/150‑1 (MQ=255)
                                                                                                                                               aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGtt      >  2:1442164/1‑150 (MQ=255)
                                                                                                                                                aCCGGCGCGCATGGCCCCGGTTTTGTGAGATAACGCTGCGGATATTCATCCTGATCAACAGCAACACACGCTACCAGTTGACCGCCGTAGTCTATTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTg     <  2:957736/150‑1 (MQ=255)
                                                                                                                                                 ccGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGa    >  2:1841104/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATGGTATTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAG  >  NC_000913/3701691‑3701987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: