Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,732,136 A→C 7.7% intergenic (+13/‑65) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,1360AC 7.7% 339.1 / 19.0 140intergenic (+13/‑65)xylF/xylGD‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base A (73/56);  new base C (5/6);  total (78/62)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-02

Only 100 of 142 total aligned reads displayed.

ACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATG  >  NC_000913/3731988‑3732285
                                                                                                                                                    |                                                                                                                                                     
aCCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCac                                                                                                                                                      >  2:822360/1‑149 (MQ=255)
   aaaaGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGCCACCGATCGATGAGAATAAAAACCACATCAACAATACGTAAATTAAAGACGGATTCCACAAAGAGAGATAGCTGTAAGCGTTACGCCCCAgcta                                                                                                                                                   >  1:312846/1‑148 (MQ=255)
   aaaaGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAgcgc                                                                                                                                                   >  1:1692744/1‑150 (MQ=255)
   aaaaGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAAAATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAgctc                                                                                                                                                   >  2:1982235/1‑148 (MQ=255)
       gCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCggcg                                                                                                                                               >  1:687951/1‑148 (MQ=255)
         aGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCg                                                                                                                                             >  2:293883/1‑150 (MQ=255)
             aCCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggggg                                                                                                                                         >  2:1116464/1‑150 (MQ=255)
              ccACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGc                                                                                                                                        >  2:1278493/1‑150 (MQ=255)
               cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCg                                                                                                                                       <  1:1730712/150‑1 (MQ=255)
               cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCg                                                                                                                                       <  2:905512/150‑1 (MQ=255)
                 cacTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGCTGTGACTAAAACCAACATCAAAGATACGGTAATTAAAGACGGATGCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGta                                                                                                                                     <  1:1054584/150‑2 (MQ=255)
                 cacTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTg                                                                                                                                     >  2:1625556/1‑150 (MQ=255)
                  acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGa                                                                                                                                    >  2:744038/1‑150 (MQ=255)
                  acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGAACGATCTGAATAAAAACATCATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTATGAGTTACGCCCCAGCGCGGAGCGGGGGCGTGa                                                                                                                                    >  1:865524/1‑150 (MQ=255)
                   cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                   >  2:961237/1‑150 (MQ=255)
                   cTGAATAATGGCCTGAAAGATGGCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGCGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                   <  1:1637412/150‑4 (MQ=255)
                    tGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAtt                                                                                                                                  <  2:250089/150‑1 (MQ=255)
                       ataatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtct                                                                                                                               <  2:932610/150‑1 (MQ=255)
                        taatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctc                                                                                                                              <  2:1383674/150‑1 (MQ=255)
                         aatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCTATCTATGTGAATAAATACAACATCAAAGATACGGTTATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctct                                                                                                                             <  2:1911373/150‑1 (MQ=255)
                           tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                           <  1:1668728/150‑1 (MQ=255)
                           tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                           <  1:511744/150‑1 (MQ=255)
                            gggcTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACTGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCa                                                                                                                          <  2:1669606/147‑1 (MQ=255)
                            ggCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTCAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCa                                                                                                                          >  2:650161/1‑150 (MQ=255)
                            ggCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCGCGGCGCGGGGGCGTGATTTGTCTCCa                                                                                                                          >  2:125768/1‑150 (MQ=255)
                             gCCTGAAAGATGTCCCCTCCCGCCTCCTGCCACCGATCGATGTGAATAAAAACAACATCAAAGCTACGGTAATTAAAGACGGATTCCCCAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCAt                                                                                                                         >  1:1100343/1‑150 (MQ=255)
                              ccTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGCGATTTCTCTCCATg                                                                                                                        >  2:585577/1‑150 (MQ=255)
                                       tGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaa                                                                                                               >  2:827544/1‑150 (MQ=255)
                                        gTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaat                                                                                                              <  2:1746970/150‑1 (MQ=255)
                                         tCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACATAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatg                                                                                                             >  1:236951/1‑150 (MQ=255)
                                          ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                            >  1:25614/1‑150 (MQ=255)
                                          ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                            >  2:178888/1‑150 (MQ=255)
                                           cccTCCCGCCTCCTGCCACCGATCGATGTGAATAAATACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatgat                                                                                                           >  2:246643/1‑149 (MQ=255)
                                               cccGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTgg                                                                                                       <  2:1437608/150‑1 (MQ=255)
                                               cccGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTgg                                                                                                       <  2:670738/150‑1 (MQ=255)
                                                     tcctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAgg                                                                                                 >  1:368548/1‑150 (MQ=255)
                                                       ctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTg                                                                                               <  1:1424356/150‑1 (MQ=255)
                                                       ctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTg                                                                                               <  1:671276/150‑1 (MQ=255)
                                                       ctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTg                                                                                               >  1:1712983/1‑150 (MQ=255)
                                                       ctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTg                                                                                               >  1:1636614/1‑150 (MQ=255)
                                                        tGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTgg                                                                                              >  1:1620035/1‑150 (MQ=255)
                                                           cacCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                           >  1:759194/1‑150 (MQ=255)
                                                              cgatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGt                                                                                        >  2:1259073/1‑150 (MQ=255)
                                                               gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                       >  2:2018758/1‑150 (MQ=255)
                                                               gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                       <  1:1069879/150‑1 (MQ=255)
                                                               gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                       >  1:166442/1‑150 (MQ=255)
                                                                atcgatGTGAATAAAAACAACATCAAAGATACTGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTa                                                                                      <  2:1681294/150‑1 (MQ=255)
                                                                 tcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTAt                                                                                     >  2:450633/1‑150 (MQ=255)
                                                                    atGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGcc                                                                                  <  1:597659/150‑1 (MQ=255)
                                                                         aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCGTTAtg                                                                             >  2:1999863/1‑149 (MQ=255)
                                                                          aTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                            >  2:1967237/1‑150 (MQ=255)
                                                                           tAAAAACAACATCAAAGAAACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTa                                                                           <  1:1820243/150‑1 (MQ=255)
                                                                            aaaaaCAACATCAAAGCTACGGTAATTAAAGACGGCTTCCACAAAGAGAGCGAGCTGGAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTAc                                                                          <  1:1754013/150‑1 (MQ=255)
                                                                             aaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAATCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                         >  2:307883/1‑150 (MQ=255)
                                                                             aaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                         >  2:780149/1‑150 (MQ=255)
                                                                             aaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                         >  1:580371/1‑150 (MQ=255)
                                                                             aaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                         >  2:1564390/1‑150 (MQ=255)
                                                                              aaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACtt                                                                        <  1:780401/150‑1 (MQ=255)
                                                                               aacaacATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGCGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTg                                                                       <  2:1338831/150‑1 (MQ=255)
                                                                                   acaTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAAt                                                                   <  2:1471304/150‑1 (MQ=255)
                                                                                   acaTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAAt                                                                   <  1:798749/150‑1 (MQ=255)
                                                                                   acaTCAAAGATACGGTAATTAAAGACGGATGCCACAAAGACTGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAAt                                                                   <  1:1443978/150‑1 (MQ=255)
                                                                                     aTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATga                                                                 <  1:1367382/150‑1 (MQ=255)
                                                                                     aTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATga                                                                 >  1:782750/1‑150 (MQ=255)
                                                                                      tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCAAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGAGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                                >  1:1877093/1‑150 (MQ=255)
                                                                                       cAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaag                                                               <  1:1283466/150‑1 (MQ=255)
                                                                                       cAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCATAGGTGGAGTCGTTATGCCTTATCTAATTGAAATgaag                                                               >  1:1205165/1‑150 (MQ=255)
                                                                                        aaaGATACGGTAATTAAAGAGGGATTCCACAAAGAGAGCTAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                              <  1:1982235/150‑1 (MQ=255)
                                                                                         aaGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaagaa                                                             <  1:1203979/150‑1 (MQ=255)
                                                                                             tACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGGGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCGTTATCTACTTGAAATGAAGAACAtt                                                         >  1:1285504/1‑150 (MQ=255)
                                                                                               cGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTTTGCCTTATCTACTTGAAATGAAGAACATTaa                                                       >  2:1841313/1‑149 (MQ=255)
                                                                                               cGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAc                                                       >  1:1772045/1‑150 (MQ=255)
                                                                                               cGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAc                                                       >  1:536616/1‑150 (MQ=255)
                                                                                                 gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                     >  2:979921/1‑150 (MQ=255)
                                                                                                       aaaGACGGATTCCACAAAGATATCGAGCTGTAAGCGTTACGCCACAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCTTTATGCCTTATCTACTTGTAATGAATAACATTTCAAAAACCt                                               >  2:1282743/1‑150 (MQ=255)
                                                                                                          gACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCg                                            >  1:498853/1‑150 (MQ=255)
                                                                                                           aCGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCGAAACCTTCgg                                           <  1:1934197/150‑1 (MQ=255)
                                                                                                              gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAg                                        <  2:680829/150‑1 (MQ=255)
                                                                                                                 tCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgtgt                                     <  2:580371/150‑1 (MQ=255)
                                                                                                                    acaAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGaa                                  >  2:1188960/1‑150 (MQ=255)
                                                                                                                        agagagCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGTATTCTCTCCATGCCGCGTGAATGAATTGGCGGAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCg                              <  2:289374/150‑1 (MQ=255)
                                                                                                                          agagCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGAt                            <  1:744038/150‑1 (MQ=255)
                                                                                                                             gcgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCAATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGa                         >  2:1400031/1‑150 (MQ=255)
                                                                                                                              cgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGACTGATTTGGCTTAGGTGGAGTCGGTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGAt                        >  1:170448/1‑150 (MQ=255)
                                                                                                                              cgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTTTGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGAt                        >  1:1861041/1‑150 (MQ=255)
                                                                                                                                agcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTTTGAAGGCGATTGATaa                      <  2:25614/150‑1 (MQ=255)
                                                                                                                                   tGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGt                   >  2:1627134/1‑150 (MQ=255)
                                                                                                                                        gCGTTACGCCCCAGCGCTGAGAGGGGGGGTGATTTCTGTCCATGCCGCGTGTATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAAGTCTGCt              <  2:1257965/150‑1 (MQ=255)
                                                                                                                                         cGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGACCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTg            >  1:916302/1‑150 (MQ=255)
                                                                                                                                          gTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTg            >  2:1376808/1‑150 (MQ=255)
                                                                                                                                          atcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGGCTTATCTACTTGAAATGAAGAACCTTTCCAAACCCTTCGGCAGTGTGAAGGCGATTGATTACGTCTGCTTg            >  1:1786558/4‑150 (MQ=255)
                                                                                                                                          atcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGCTAACGTCTGCTTg            >  2:398560/4‑150 (MQ=255)
                                                                                                                                          atcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTg            >  2:1323497/4‑150 (MQ=255)
                                                                                                                                          atcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTATGCTTg            >  1:1491281/4‑150 (MQ=255)
                                                                                                                                           ttACGCCCCAGCGCTGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAAGGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGc           <  1:148070/150‑1 (MQ=255)
                                                                                                                                             aCGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAACTTCGGCAGTGTGAAGGCGATTGATAACGTCGGCTTGCgg         >  2:530496/1‑150 (MQ=255)
                                                                                                                                               gCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAATTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGtt       <  1:238187/150‑1 (MQ=255)
                                                                                                                                                cccgAGCGCGTAGGGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTTGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTCCCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTg      <  2:389433/150‑1 (MQ=255)
                                                                                                                                                ccccAGCGCGGGGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTg      <  2:1895194/150‑1 (MQ=255)
                                                                                                                                                ccccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTTTGGCTTATCTACGTGAAATGAAGAACATTACCAAAACCTTGCGCAGTGTGAAGGCGAATGATAACCTCTGCTTGCGGttt      >  2:913075/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATG  >  NC_000913/3731988‑3732285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: