Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 15,318 G→A 5.0% intergenic (+20/‑127) dnaJ → / → insL1 chaperone Hsp40, DnaK co‑chaperone/IS186 transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091315,3180GA 5.0% 306.0 / 2.5 100intergenic (+20/‑127)dnaJ/insL1chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
Reads supporting (aligned to +/- strand):  ref base G (48/46);  new base A (3/2);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

Only 100 of 103 total aligned reads displayed.

CGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC  >  NC_000913/15169‑15465
                                                                                                                                                     |                                                                                                                                                   
cGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGCGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGCTGGTGTGAAGTAGTTTTTTGACGACCTGCCCCGCTAACCTCCCCAAAAGCCTTCCCg                                                                                                                                                     >  1:1176444/1‑150 (MQ=255)
 gAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCACCCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGGTTTTTGACGACGTGACCCGCTAACCTCCCCAAAAGCCTGCCCgg                                                                                                                                                    >  2:384488/1‑149 (MQ=255)
 gAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCgg                                                                                                                                                    >  2:1558714/1‑149 (MQ=255)
   aaGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGCCCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTgg                                                                                                                                                  >  2:1033443/1‑150 (MQ=255)
    aGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCACGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGc                                                                                                                                                <  2:648446/150‑1 (MQ=255)
     ggCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCCCAACAGCCCGCGCTCAAAGCGCTGCTTTGAAGGTGTGAAGAAGTTTTTTGCAGACCTGACCAGATAACCTCCCCTAAAGCCTGCCCCTGGGc                                                                                                                                                >  2:339347/1‑150 (MQ=255)
         gAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGc                                                                                                                                            >  2:779463/1‑150 (MQ=255)
         gAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGATCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTTACCTCCACAAACGCCTGCCCGTGGGCAGGc                                                                                                                                            >  1:1093483/1‑150 (MQ=255)
          acaccgctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCACCCGGCGAGCACATCAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAACAGCCTGCCCGTGGGCAGGcc                                                                                                                                           <  2:355807/145‑1 (MQ=255)
            accgctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGGGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTg                                                                                                                                         <  2:1836285/147‑1 (MQ=255)
              aGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCACGGGCAGGcttttg                                                                                                                                       <  2:1629460/150‑6 (MQ=255)
               gctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTCACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTgggg                                                                                                                                      >  1:1727975/1‑149 (MQ=255)
               gctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGt                                                                                                                                      >  2:519520/1‑150 (MQ=255)
               gctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCACGGGCAGGcttttgg                                                                                                                                      <  2:230071/150‑7 (MQ=255)
                    ttctagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGTTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTaaaaa                                                                                                                                 <  2:513512/146‑1 (MQ=255)
                    tgcaagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTaaaaa                                                                                                                                 >  2:443229/1‑150 (MQ=255)
                        agagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg                                                                                                                             <  1:361719/150‑1 (MQ=255)
                        agagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg                                                                                                                             <  1:795443/150‑1 (MQ=255)
                         gagctgcaagaAAGCTTCGGGGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAggg                                                                                                                            <  1:1595726/150‑1 (MQ=255)
                              gcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACCACAGCCCGCGCTCAAAGAGCGTCGTTGATGGTGTGAAGAAGTTTTGTGCCGACCGGCCCCGCTAACCTCCCCAACCGCCTGCCCGTGGGCAGGCCTGGGTAACAATAGGGTGCGt                                                                                                                       >  1:106121/1‑150 (MQ=255)
                              gcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGCCCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGt                                                                                                                       >  2:1980772/1‑150 (MQ=255)
                              gcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGt                                                                                                                       >  1:1172737/1‑150 (MQ=255)
                                  gaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGaa                                                                                                                   <  1:1552941/150‑1 (MQ=255)
                                   caaGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAg                                                                                                                  <  2:278852/149‑1 (MQ=255)
                                    aaGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAATAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGa                                                                                                                 <  1:1750767/150‑1 (MQ=255)
                                    aaGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGa                                                                                                                 <  2:1221939/150‑1 (MQ=255)
                                      gCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGCCGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCGTGGGTAAAACTAGGGTGCGTTGAAGata                                                                                                               >  1:111270/1‑150 (MQ=255)
                                       cTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGatat                                                                                                              >  1:1327975/1‑150 (MQ=255)
                                       cTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGatat                                                                                                              >  1:854074/1‑150 (MQ=255)
                                         tCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGc                                                                                                            <  1:1160115/150‑1 (MQ=255)
                                           ggTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCgc                                                                                                          >  2:1004104/1‑149 (MQ=255)
                                            gTGGCCCAACCGGCGAGCACAACAGCCCCCGCTCAAAGAGCTGCTTTGATGGTGTGAAGACGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAg                                                                                                         <  1:110444/150‑1 (MQ=255)
                                                  cAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTg                                                                                                   >  2:1087878/1‑150 (MQ=255)
                                                   aaCCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCTGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGt                                                                                                  <  2:1635442/150‑1 (MQ=255)
                                                      cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGAAGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTaaa                                                                                               <  1:560524/150‑1 (MQ=255)
                                                         cGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTg                                                                                            >  2:1528120/1‑150 (MQ=255)
                                                            gCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCGGTAAAGTGGCg                                                                                         >  1:1013792/1‑150 (MQ=255)
                                                             cacaACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgg                                                                                        <  1:585937/150‑1 (MQ=255)
                                                              acaacaGCCCGCTCTCAAAGAGCTTCTTTGATGGGGTGAAGAAGTGTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGGTGAAGATATGCGAGCACCTGTAAAGTGGCggg                                                                                       <  1:1289332/150‑1 (MQ=255)
                                                              acaacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTATAAATAGGGTGCGTTGAAGATATGAGAGCACCTGTAAAGTGGCggg                                                                                       >  1:1303932/1‑150 (MQ=255)
                                                              acaacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCggg                                                                                       <  1:645869/150‑1 (MQ=255)
                                                               caacaGCCCGCGCTCAAAGCGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                                      <  1:1633328/150‑1 (MQ=255)
                                                               caacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAACAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                                      <  2:794361/150‑1 (MQ=255)
                                                               caacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAATAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                                      >  1:51556/1‑150 (MQ=255)
                                                               caacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATATGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                                      <  2:1866550/150‑1 (MQ=255)
                                                               caacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                                      >  2:1112656/1‑150 (MQ=255)
                                                               caacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                                      <  1:1624944/150‑1 (MQ=255)
                                                                  caGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCACGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTTAAGTGGCGGGGATc                                                                                   >  2:312645/1‑150 (MQ=255)
                                                                    gcccgcccTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGTGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCAc                                                                                 <  2:159707/150‑1 (MQ=255)
                                                                      ccGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTc                                                                               >  2:64394/1‑150 (MQ=255)
                                                                      ccGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTc                                                                               >  2:1221373/1‑150 (MQ=255)
                                                                         cgcTCAAAGAGCGTCTTTGATGGTGGGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCa                                                                            <  1:818623/150‑1 (MQ=255)
                                                                           cTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATa                                                                          >  1:1160233/1‑150 (MQ=255)
                                                                            tCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATaa                                                                         <  2:843522/150‑1 (MQ=255)
                                                                              aaaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGCGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAgc                                                                       <  1:760380/150‑1 (MQ=255)
                                                                                   gCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTaa                                                                  >  2:1061121/1‑150 (MQ=255)
                                                                                        tttGATGGTGTGAAGAAGTTTTTTGACGACCTCACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAACATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTaa                                                             <  1:453208/150‑1 (MQ=255)
                                                                                            aTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCGGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGt                                                         >  2:1011868/1‑150 (MQ=255)
                                                                                              ggTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtg                                                       <  2:223569/150‑1 (MQ=255)
                                                                                               gtgtGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgt                                                      >  2:649708/1‑150 (MQ=255)
                                                                                                ggtgAAGAAGTCTTATGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg                                                     <  2:771683/149‑1 (MQ=255)
                                                                                                  tgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCATGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGTGAACACTCCCATAAGCGATAACTTAAGGGTTGTGGt                                                   >  2:690678/1‑150 (MQ=255)
                                                                                                   gaagcaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAACAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAGGGGTTGTGGTa                                                  <  1:274647/150‑1 (MQ=255)
                                                                                                   gaagaaGTTTTTTGACGACCTGCCCCGCTAACCTCCCCAAACGCCTGGCCGTGGGGAGGCCTGGGTTAGATTTGGGTGCGTGGAAGTTTTGCGGGCACCTGGAAAGTGGCGGGGTTCACTCCCTTTAGCGCTATCGTAAGGGGTGTgggg                                                  >  2:1330286/1‑148 (MQ=255)
                                                                                                   gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCACATAAGCGCTAACTTAAGGGTTGTGGTa                                                  >  2:1248681/1‑150 (MQ=255)
                                                                                                     agaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                                >  2:1110732/1‑150 (MQ=255)
                                                                                                         gTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                            <  2:2015164/150‑1 (MQ=255)
                                                                                                           tttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCt                                          <  1:779463/150‑1 (MQ=255)
                                                                                                           tttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCt                                          >  1:426503/1‑150 (MQ=255)
                                                                                                            ttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGTTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTg                                         <  2:527982/150‑1 (MQ=255)
                                                                                                            ttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTg                                         <  1:1371339/150‑1 (MQ=255)
                                                                                                             tttGACGACCGGCCCCGCTACCCTCCCCAATAGCCGGCCCGGGGGCAGGCCTGGGTAAAAATAGGGTGCGGTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                        <  1:164767/150‑1 (MQ=38)
                                                                                                                   gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAt                                  >  2:408355/1‑150 (MQ=255)
                                                                                                                    acCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAtt                                 >  1:1661729/1‑150 (MQ=255)
                                                                                                                     ccTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                                >  1:1615515/1‑150 (MQ=255)
                                                                                                                         aCCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACg                            <  1:519520/150‑1 (MQ=255)
                                                                                                                          cccGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGt                           >  2:490016/1‑150 (MQ=255)
                                                                                                                           ccGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTg                          >  1:1670631/1‑150 (MQ=255)
                                                                                                                            cGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGGAAAAATCGGGGGCGTGGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGc                         <  2:1727975/150‑1 (MQ=255)
                                                                                                                             gCTAAGCTCCCCACAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGGTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGcc                        <  2:297942/150‑1 (MQ=255)
                                                                                                                               tAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATAAGCGAGCACCTGTAAAGTGGCGGGGAGCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCGGa                      >  1:1446463/1‑150 (MQ=255)
                                                                                                                                aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                     >  2:1499098/1‑150 (MQ=255)
                                                                                                                                 aCCTCCCCAACAGCCTGCCCGTGGGCAGGCCTGTGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAATGTGGCGTGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATg                    <  2:992630/150‑1 (MQ=255)
                                                                                                                                   cTCCCCAACAGCCTGCCCGTGGGCAGGCATGGGTATAAATAGGGTGCGTTGAAGATATGCGAGAACATGGAAAGTGGCGGGGATCACTCCCATAAGCGGTAACTTAAGGGTTGTGGTAGTACGCGTGATTTGATTTAACGTGCCGATGat                  >  2:370849/1‑149 (MQ=255)
                                                                                                                                    tCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                 <  2:1922330/150‑1 (MQ=255)
                                                                                                                                    tCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                 <  1:1997106/150‑1 (MQ=255)
                                                                                                                                     ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAtt                <  1:1033443/150‑1 (MQ=255)
                                                                                                                                         ccaggCCTGCCCACGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGATTTActc            >  1:1539687/5‑150 (MQ=255)
                                                                                                                                         aaaaGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActc            <  1:64394/150‑1 (MQ=255)
                                                                                                                                            ggCCTGCCCACGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCCCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGGCTGATATGATTTAACGTGCCGCTGAATTACTCTCa         >  1:357498/2‑150 (MQ=25)
                                                                                                                                            aGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa         >  1:404696/1‑150 (MQ=255)
                                                                                                                                             gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAc        >  1:1424450/1‑150 (MQ=255)
                                                                                                                                             gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAc        >  2:43898/1‑150 (MQ=255)
                                                                                                                                              ccTGCCCACGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCTAGCACCTGTAACGTGGCGGGGAGCACTCCCATAAGCGATAACTTAAGGGTTGTGGTATTAAGCCTGTGAGGATTTAACGGGCCGAGGAATTACTCTCACg       >  2:77985/1‑150 (MQ=21)
                                                                                                                                               cTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGa      >  2:1894505/1‑150 (MQ=255)
                                                                                                                                                tGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAt     >  2:984180/1‑150 (MQ=255)
                                                                                                                                                 gCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa    <  2:1103282/150‑1 (MQ=255)
                                                                                                                                                 gCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCAATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa    >  1:1808341/1‑150 (MQ=255)
                                                                                                                                                  cccGTGGGCAGGCCTTGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATaa   <  1:814837/150‑1 (MQ=255)
                                                                                                                                                   ccGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAc  >  2:121097/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC  >  NC_000913/15169‑15465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: