BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,748,108	T→A	5.0%	F342Y (TTC→TAC)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,108	0	T	A	5.0%	327.6 / 2.5	111	F342Y (TTC→TAC)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base T (53/51); new base A (2/4); total (55/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GTGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTA  >  NC_000913/3747961‑3748257

gTGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTcc                                                                                                                                                     <  1:1169516/150‑1 (MQ=255)

gTGAATTTATAGTTCACGAAGCGAGCCCTACCTCTTCCTGCAACCTCGAAGGGTTTACAGCACAGTGGGGAGATATCTCGTTTGATGAGATCAATCAGGCCGTTTCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTcc                                                                                                                                                     <  2:1185809/150‑1 (MQ=255)

 tGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCt                                                                                                                                                    >  2:1161713/1‑150 (MQ=255)

  taaTTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTATCCTGCCGTACCTg                                                                                                                                                   <  2:61534/149‑1 (MQ=255)

  gAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTg                                                                                                                                                   <  2:617969/150‑1 (MQ=255)

     tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                                <  2:1829350/150‑1 (MQ=255)

          cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcag                                                                                                                                           <  2:1676494/150‑1 (MQ=255)

          cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGATTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcag                                                                                                                                           <  1:1347663/150‑1 (MQ=255)

           gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                          <  2:1621911/150‑2 (MQ=255)

           gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCGTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                          >  1:1466520/1‑150 (MQ=255)

             tCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGCAATCTCGGTTGATGAGATCTATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATGTCTTTTTCCTGCCGGTCCTGTACGGCAGCaa                                                                                                                                        >  1:297799/1‑150 (MQ=255)

              cACGAAGCCAGCCCTACCTCTTCCGGCACCCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTGTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAAc                                                                                                                                       <  1:238699/150‑1 (MQ=255)

               aCGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaaa                                                                                                                                      <  2:232250/150‑3 (MQ=255)

               aCGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaaa                                                                                                                                      <  2:225976/150‑3 (MQ=255)

               aCGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGCGGAGCAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCCACg                                                                                                                                      >  1:326945/1‑150 (MQ=255)

                  aaGCCAGCCCTACCTCTTCCGGCACCCCCGCAGGGATTCCCGCACAGTGGCGAGAAATCACGTTTGATGAGATCAATCAGGCCGCTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCg                                                                                                                                   <  2:303715/150‑1 (MQ=255)

                   agccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCgg                                                                                                                                  <  1:588565/150‑1 (MQ=255)

                      cagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATATCATTTGATGAGATCAATCAGGCCGTTGCAAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGAca                                                                                                                               >  2:301936/1‑149 (MQ=255)

                        gccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCg                                                                                                                             <  2:1253582/150‑1 (MQ=255)

                        gccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCg                                                                                                                             <  1:606469/150‑1 (MQ=255)

                         cccTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCga                                                                                                                            >  2:1843752/1‑150 (MQ=255)

                            tACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGAt                                                                                                                         >  1:1884192/1‑150 (MQ=255)

                            tACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGAt                                                                                                                         <  2:7061/150‑1 (MQ=255)

                               ctctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAc                                                                                                                      >  1:1363146/1‑150 (MQ=255)

                                gctTCCGGCAACCTCGAATGGGATACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGGGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                     <  2:177670/149‑1 (MQ=255)

                                   tCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGt                                                                                                                  <  2:2009102/150‑1 (MQ=255)

                                     cGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTgg                                                                                                                <  1:598279/150‑1 (MQ=255)

                                     cGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTgg                                                                                                                <  1:1749706/150‑1 (MQ=255)

                                       gCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGtt                                                                                                              >  2:211976/1‑150 (MQ=255)

                                        cAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGttt                                                                                                             >  1:906757/1‑150 (MQ=255)

                                         aaCCTCGAATGGTTTACCGCACAGTGAGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTc                                                                                                            >  2:1931624/1‑150 (MQ=255)

                                              cGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGCCTCGAGATGACCAGTGGTTTCTACgg                                                                                                       >  1:528430/1‑150 (MQ=255)

                                              cGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACgg                                                                                                       >  1:1889403/1‑150 (MQ=255)

                                              cGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGACAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACgg                                                                                                       <  2:1238219/150‑1 (MQ=255)

                                               gtaTGGTTTACCGCACAGTGGGGAGAATTGTCGTTTGATGAGATCTATGAGGCCGTTGCCAGCTTGCCGAAGGCGGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCTGTGGTTTCTACggg                                                                                                      <  1:1414930/148‑1 (MQ=255)

                                                 aTGGTTTACCGCACAGTGAGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGAt                                                                                                    <  1:1931624/150‑1 (MQ=255)

                                                    gTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCa                                                                                                 >  1:1319859/1‑150 (MQ=255)

                                                     tttACCGCACAGTGGGGAGAAATCTCTTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAg                                                                                                <  2:1500036/150‑1 (MQ=255)

                                                       tACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGc                                                                                              >  1:23146/1‑150 (MQ=255)

                                                           gCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCAta                                                                                          >  2:1218094/1‑149 (MQ=255)

                                                            cacaGTGGGGAGAAATCTCGTTTGATGAGATCATTCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTc                                                                                         <  2:615433/150‑1 (MQ=255)

                                                            cacaGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTc                                                                                         <  2:1120425/150‑1 (MQ=255)

                                                                gTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGGTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCacac                                                                                     <  1:1172378/150‑1 (MQ=255)

                                                                gTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCacac                                                                                     <  2:1977364/150‑1 (MQ=255)

                                                                  ggggTGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACAccc                                                                                   >  1:1156593/1‑150 (MQ=255)

                                                                  ggggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCATAccc                                                                                   >  1:941553/1‑150 (MQ=255)

                                                                   gggAGAAATCTCGTTTGATGAGATCACTCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTATATCCTGCCGTTACTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcg                                                                                  >  1:1146043/1‑150 (MQ=255)

                                                                   gggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACTGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACccccc                                                                                  >  1:1721676/1‑149 (MQ=255)

                                                                    ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgc                                                                                 >  1:423059/1‑150 (MQ=255)

                                                                     gagaAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgcg                                                                                >  1:1215797/1‑150 (MQ=255)

                                                                       gaAATCTCGTTTGATGAGATCAATCAGGTCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgcgcg                                                                              <  2:7676/150‑1 (MQ=255)

                                                                        aaaTCTGGTGTGATGCGATCACTCAGGCCGTTGCCAGGTCGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTGACTACGGGATGCAGGCCATTCACACCCgcgcgc                                                                             <  2:326945/150‑1 (MQ=255)

                                                                         aaTCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGGTCCTGTACGGCAGCATCGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGGAGGCCCTTCACCCCCgcgcgcg                                                                            >  1:266382/1‑149 (MQ=255)

                                                                              cGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGt                                                                       >  1:875723/1‑150 (MQ=255)

                                                                                 ttGATGAGATCAATCAGGCCGTTGCCAGCATGACGAAGGCTGGGGGCGATCTCTTTATCCTGCCGTTCCTGTACGTCAGCAACGCCTGACTCGAGATGACCAGTGGTTTCAACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGc                                                                    <  1:318355/150‑1 (MQ=255)

                                                                                    atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                                 <  2:1889140/150‑1 (MQ=255)

                                                                                    atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGAACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                                 <  1:1998230/150‑1 (MQ=255)

                                                                                    atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGACGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                                 <  1:301936/150‑1 (MQ=255)

                                                                                     tgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGc                                                                >  2:142814/1‑150 (MQ=255)

                                                                                      gagaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGcc                                                               >  2:26197/1‑150 (MQ=255)

                                                                                      gagaTCAATCAGGCCGTTGCCAGCATGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGCCCAGAGGTTACTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGTcc                                                               >  2:1426798/1‑150 (MQ=255)

                                                                                       agaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCa                                                              >  1:1838531/1‑150 (MQ=255)

                                                                                        gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                             <  2:1891922/150‑1 (MQ=255)

                                                                                        gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                             >  1:803233/1‑150 (MQ=255)

                                                                                               caGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGaa                                                      >  1:1376560/1‑150 (MQ=255)

                                                                                                   ccGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAgggg                                                  <  2:637819/150‑1 (MQ=255)

                                                                                                   ccGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGGACGGCAGCAACGCCGGACTCGAGATGAGCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAgggg                                                  <  1:786936/150‑1 (MQ=255)

                                                                                                   ccGATGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGACATTCACACCCCCGCGCACCTGTAGAACGCCATCTAAGAAgggg                                                  >  2:1542298/1‑150 (MQ=255)

                                                                                                     gTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtg                                                <  1:1931105/150‑1 (MQ=255)

                                                                                                      ttGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGGACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGAGGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtgg                                               <  2:1962856/150‑1 (MQ=255)

                                                                                                      gtGCCAGCTGGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtgg                                               <  1:1333110/149‑1 (MQ=255)

                                                                                                          cAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGtt                                           >  2:1266903/1‑150 (MQ=255)

                                                                                                            gCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTTCGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCa                                         <  2:1378780/150‑1 (MQ=255)

                                                                                                             cTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                        <  2:1544275/150‑1 (MQ=255)

                                                                                                             cTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAACTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCat                                        >  2:1476050/1‑149 (MQ=255)

                                                                                                              ttGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGc                                       <  1:1161713/150‑1 (MQ=255)

                                                                                                                gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                     <  2:1889403/150‑1 (MQ=255)

                                                                                                                gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGACCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                     <  2:1363146/150‑1 (MQ=255)

                                                                                                                 ccGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat                                    >  1:1115810/1‑150 (MQ=255)

                                                                                                                  cGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCata                                   <  1:648385/150‑1 (MQ=255)

                                                                                                                                 aTCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAAcc                    >  2:40746/1‑150 (MQ=255)

                                                                                                                                   ctctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACCCCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCCCCTCAACCGa                  >  1:1890693/1‑150 (MQ=255)

                                                                                                                                   ctctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                  >  2:1866979/1‑150 (MQ=255)

                                                                                                                                   ctctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                  >  2:1859285/1‑150 (MQ=255)

                                                                                                                                   ctctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                  >  2:587357/1‑150 (MQ=255)

                                                                                                                                    tctTTTTCCTGCGGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAGCAGTGGTTTCTACGGGATGCATGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTAGGAAGGGGTGGTGTTCCGCCAGATGGCCCGCCTCAAcaggc                 >  1:73345/1‑146 (MQ=255)

                                                                                                                                     ctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCCCACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCCCCTCAACCGAAt                >  2:1933562/1‑150 (MQ=255)

                                                                                                                                     ctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAAt                >  1:845904/1‑150 (MQ=255)

                                                                                                                                       cgttgcTGCCGTACAGGAACGGCAGGAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATgc              >  1:862669/6‑150 (MQ=255)

                                                                                                                                          tCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCCGCCATATGACCCACCTCAAACGAATGcgcg           >  1:1645477/1‑150 (MQ=255)

                                                                                                                                          tCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATCACCCACCTCAACCGAATGcgcg           >  1:1855865/1‑150 (MQ=255)

                                                                                                                                           ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCCCCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa          >  1:1971001/1‑150 (MQ=255)

                                                                                                                                           ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa          >  2:1237321/1‑150 (MQ=255)

                                                                                                                                           ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAACGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa          <  1:1241472/150‑1 (MQ=255)

                                                                                                                                            cTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGaa         >  1:1287316/1‑150 (MQ=255)

                                                                                                                                             tGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAGGGGATGCAGGCCAGTCACACCCGCGCGCACCTGATGCCGGGCATCTATGCAGGGGTGGTGTTCAGCCATATGACCACCCTCACCCGCATGCGCGAAc        >  1:164041/1‑150 (MQ=255)

                                                                                                                                             tGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAAc        <  2:1340156/150‑1 (MQ=255)

                                                                                                                                                cGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGtt     >  2:1034404/1‑150 (MQ=255)

                                                                                                                                                  ttCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGtttt   <  2:206025/150‑1 (MQ=255)

                                                                                                                                                   tCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATACACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGTGTGGTGTTCATCCATATGACCCACCTCAAACGAATCCGAGAACGGTTTa  >  2:1250548/1‑150 (MQ=255)

GTGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTA  >  NC_000913/3747961‑3748257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`