Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,777,135 T→C 6.4% intergenic (+108/‑264) yibL → / → lldP ribosome‑associated DUF2810 family protein/L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,777,1350TC 6.4% 372.9 / 11.9 125intergenic (+108/‑264)yibL/lldPribosome‑associated DUF2810 family protein/L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base T (64/53);  new base C (6/2);  total (70/55)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

Only 100 of 131 total aligned reads displayed.

GGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATT  >  NC_000913/3776986‑3777284
                                                                                                                                                     |                                                                                                                                                     
 gCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATtc                                                                                                                                                      <  2:984673/150‑1 (MQ=255)
  ccTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATtct                                                                                                                                                     >  2:708565/1‑150 (MQ=255)
   cggCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATtctc                                                                                                                                                    <  2:240362/148‑1 (MQ=255)
    ggCAGGAGATGACTGGGTTTTCAAAGCCCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTctct                                                                                                                                                   <  2:1847732/149‑1 (MQ=255)
        ggAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCtgatg                                                                                                                                               >  2:1455708/1‑150 (MQ=255)
         gagaTGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCtgatga                                                                                                                                              <  1:559168/150‑1 (MQ=255)
          agaTGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAg                                                                                                                                             <  2:829080/150‑1 (MQ=255)
               aCTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTg                                                                                                                                        >  2:1423138/1‑150 (MQ=255)
                 tGGGTTTTCAATGACCGCGTTTTAAGAACTCAGTATCTACAGGGTGATTCTGCACATTCCTTTCGGCCGAGTAAGGTGTTGTCGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGcc                                                                                                                                      <  2:765323/150‑1 (MQ=255)
                  gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                     >  1:432725/1‑150 (MQ=255)
                  gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                     <  2:1729005/150‑1 (MQ=255)
                  gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                     <  1:739115/150‑1 (MQ=255)
                  gggTATTCAAAGACCGAGATTAAAGAACACAGTATATACAGGGTGATTCTGCACATTACTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATCCTCATCAGAGAATTGccc                                                                                                                                     <  2:366506/150‑1 (MQ=255)
                   ggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATCCTCATCAGAGAATTGCCCt                                                                                                                                    <  1:1882800/150‑1 (MQ=255)
                       ttCAAAGACCGCGTTTTAAGAAGACAGTATCTACAGGGGGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTc                                                                                                                                <  1:1146198/150‑1 (MQ=255)
                        tCAAAGACCTCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATGCCTATAGGCCGAGTAAGTTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCt                                                                                                                               <  2:812602/150‑1 (MQ=255)
                         cAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCtt                                                                                                                              <  2:838488/150‑1 (MQ=255)
                          aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTTCACTTTTCttt                                                                                                                             >  1:256201/1‑150 (MQ=255)
                          aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                             >  2:1618910/1‑150 (MQ=255)
                          aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                             >  2:9343/1‑150 (MQ=255)
                            aGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAc                                                                                                                           >  1:680476/1‑150 (MQ=255)
                               ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCTCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                        <  2:673881/150‑1 (MQ=255)
                               ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                        >  1:1333075/1‑150 (MQ=255)
                               ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                        <  1:2005931/150‑1 (MQ=255)
                                cgcgTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGa                                                                                                                       <  1:670948/150‑1 (MQ=255)
                                     tttAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACAtct                                                                                                                  >  2:813566/1‑150 (MQ=255)
                                       tAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTcc                                                                                                                >  2:587609/1‑150 (MQ=255)
                                                aGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAg                                                                                                       >  2:976212/1‑150 (MQ=255)
                                                aGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAg                                                                                                       <  2:112899/150‑1 (MQ=255)
                                                     cTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTg                                                                                                  >  2:1633758/1‑150 (MQ=255)
                                                     cTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTg                                                                                                  >  2:451722/1‑150 (MQ=255)
                                                      tACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTgg                                                                                                 >  2:1918/1‑150 (MQ=255)
                                                      tACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTgg                                                                                                 >  1:997546/1‑150 (MQ=255)
                                                       aCAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGc                                                                                                <  1:514568/150‑1 (MQ=255)
                                                        cAGGGTGATTCAGCAAATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGcc                                                                                               <  1:269509/150‑1 (MQ=255)
                                                         aGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGccc                                                                                              <  2:1939989/150‑1 (MQ=255)
                                                           ggTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTa                                                                                            >  1:1982615/1‑150 (MQ=255)
                                                              gATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCa                                                                                         >  1:559660/1‑150 (MQ=255)
                                                                    gCACATTCCTATAGGCCGAGTAAGGTGTTCACGCGGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAAttctt                                                                                   >  1:1293670/1‑150 (MQ=255)
                                                                    gCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAAttctt                                                                                   >  2:946055/1‑150 (MQ=255)
                                                                       caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                <  2:1136298/150‑1 (MQ=255)
                                                                       caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                <  1:1408663/150‑1 (MQ=255)
                                                                       caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                <  2:1071203/150‑1 (MQ=255)
                                                                         ttCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTACCCCCACAAGAATTGGCCCTACCAATTGTTCGCtt                                                                              >  1:1745596/1‑150 (MQ=255)
                                                                         ttCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCGTTTCTTTACCAGACAGCTCCCCCCACAAGAATTGGCCCTACCAATTCGTCGCtt                                                                              >  1:1753440/1‑150 (MQ=255)
                                                                           ccTAAAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTAt                                                                            >  1:892653/1‑150 (MQ=255)
                                                                            cTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCGATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATc                                                                           >  2:439551/1‑150 (MQ=255)
                                                                             tataGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCt                                                                          >  2:1598864/1‑150 (MQ=255)
                                                                             tataGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTACGCTTTTCt                                                                          >  2:1123495/1‑150 (MQ=255)
                                                                                    cGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACctct                                                                   >  2:411765/1‑150 (MQ=255)
                                                                                      aGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTgg                                                                 <  2:461299/150‑1 (MQ=255)
                                                                                        tAAGGTGTTCACGCCGCATCCTGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCGACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGtt                                                               <  2:371207/150‑1 (MQ=255)
                                                                                         aaGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGACTTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTc                                                              >  2:59253/1‑150 (MQ=255)
                                                                                           ggTGTTCTCGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcac                                                            <  1:710555/150‑1 (MQ=255)
                                                                                           ggTGTTCACGCCGCATCCGCCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAAATCTCTGAAGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGACAAACCAATTCTTCGCTTATAAGACCTCTGGTTcaa                                                            >  2:339017/1‑149 (MQ=255)
                                                                                            gtgtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcaca                                                           <  2:550537/150‑1 (MQ=255)
                                                                                              gtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAAt                                                         >  2:767751/1‑150 (MQ=255)
                                                                                                tCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAAttt                                                       >  1:544615/1‑150 (MQ=255)
                                                                                                 cACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTc                                                      >  2:669645/1‑150 (MQ=255)
                                                                                                   cgccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTccc                                                    >  2:1267822/1‑150 (MQ=255)
                                                                                                    gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACACGAATTGGCCCTACCAATTCTTCGCGTAGCTGACCTCTGGGTCCCAATTTcccc                                                   >  2:1454863/1‑149 (MQ=255)
                                                                                                    gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCa                                                   <  1:855692/150‑1 (MQ=255)
                                                                                                    gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTACCa                                                   >  1:1749606/1‑150 (MQ=255)
                                                                                                     ccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTTATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTTTCTGACCTCTGGTTCACAATTTCCCaa                                                  <  1:911865/150‑1 (MQ=255)
                                                                                                      cgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAAt                                                 <  2:1991441/150‑1 (MQ=255)
                                                                                                         aTCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCTACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaa                                              <  1:1319607/150‑1 (MQ=255)
                                                                                                          tCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaa                                             <  2:1333075/150‑1 (MQ=255)
                                                                                                           ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                            >  2:188800/1‑150 (MQ=255)
                                                                                                           ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                            >  2:1838168/1‑150 (MQ=255)
                                                                                                           ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                            <  2:1063802/150‑1 (MQ=255)
                                                                                                            cGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCAGACCTCTGGTTCAAAATTACACAATTaaaaa                                           >  2:904025/1‑149 (MQ=255)
                                                                                                             ggCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACt                                          <  1:579998/150‑1 (MQ=255)
                                                                                                                  gATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACAt                                     >  2:1778933/1‑150 (MQ=255)
                                                                                                                    tAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCa                                   <  2:922604/150‑1 (MQ=255)
                                                                                                                       ggCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATg                                >  2:1339018/1‑150 (MQ=255)
                                                                                                                            tctGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGcc                           <  1:1145595/150‑1 (MQ=255)
                                                                                                                               ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGCCCGCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                        >  1:1235272/1‑150 (MQ=255)
                                                                                                                               ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                        >  2:1733146/1‑150 (MQ=255)
                                                                                                                               ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                        >  1:184684/1‑150 (MQ=255)
                                                                                                                                gATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATa                       <  1:1423138/150‑1 (MQ=255)
                                                                                                                                 aTCAACACCCTCACGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATAc                      <  2:1923557/150‑1 (MQ=255)
                                                                                                                                 aTCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATAc                      >  2:857554/1‑150 (MQ=255)
                                                                                                                                  tCAACAACCTAATGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACa                     <  1:2004208/150‑1 (MQ=255)
                                                                                                                                  tCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACa                     >  2:213688/1‑150 (MQ=255)
                                                                                                                                   caacaaCCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAt                    <  2:997546/150‑1 (MQ=255)
                                                                                                                                   caacaaCCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACATCTGGTTAACAATTTCCCAAATAAAACTAACAAAAAAGTTGCCAATACAt                    >  1:374365/1‑150 (MQ=255)
                                                                                                                                     acaacCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATaa                  >  1:476929/1‑150 (MQ=255)
                                                                                                                                      caacCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAAc                 <  2:680476/150‑1 (MQ=255)
                                                                                                                                      aaagaaaaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAAc                 >  2:1094594/7‑150 (MQ=38)
                                                                                                                                        agaaaaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACAt               >  1:1186324/5‑150 (MQ=255)
                                                                                                                                          cTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTa            <  1:1430806/150‑1 (MQ=255)
                                                                                                                                           tAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAAAACATTTa            <  1:1363358/150‑1 (MQ=255)
                                                                                                                                           aaaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTATTCGCTTATCTGACATCTGGTTCACAATTTCCCAATTAAAACTAACATCAATGATGCCACTACATAACATTTa            >  1:954107/2‑150 (MQ=255)
                                                                                                                                            aaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAg           >  2:693857/1‑150 (MQ=255)
                                                                                                                                             aGGGCATTTCTCTGATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCACACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt          <  1:159334/150‑1 (MQ=255)
                                                                                                                                             aGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt          <  1:1392846/150‑1 (MQ=255)
                                                                                                                                             aGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt          >  2:152976/1‑150 (MQ=255)
                                                                                                                                               ggCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACAAAATATTCCCTTTTCTGACATCGGGTTCACAATTTCCCAATTAAAAATCACATCAATGTTGCCAAAACAAAACATTTAGTTa        >  1:1847616/1‑150 (MQ=255)
                                                                                                                                                   aTTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAAAca    >  1:861911/1‑150 (MQ=255)
                                                                                                                                                     tctcTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCAGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcatt  >  1:1353926/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCCTGCAGGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATT  >  NC_000913/3776986‑3777284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: