Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,777,142 T→A 6.8% intergenic (+115/‑257) yibL → / → lldP ribosome‑associated DUF2810 family protein/L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,777,1420TA 6.8% 374.2 / 11.2 127intergenic (+115/‑257)yibL/lldPribosome‑associated DUF2810 family protein/L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base T (65/53);  new base A (7/2);  total (72/55)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTC  >  NC_000913/3776994‑3777291
                                                                                                                                                    |                                                                                                                                                     
ggAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCtgatg                                                                                                                                                      >  2:1455708/1‑150 (MQ=255)
 gagaTGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCtgatga                                                                                                                                                     <  1:559168/150‑1 (MQ=255)
  agaTGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAg                                                                                                                                                    <  2:829080/150‑1 (MQ=255)
         tGGGTTTTCAATGACCGCGTTTTAAGAACTCAGTATCTACAGGGTGATTCTGCACATTCCTTTCGGCCGAGTAAGGTGTTGTCGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGcc                                                                                                                                             <  2:765323/150‑1 (MQ=255)
          gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                            <  2:1729005/150‑1 (MQ=255)
          gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                            <  1:739115/150‑1 (MQ=255)
          gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                            >  1:432725/1‑150 (MQ=255)
          gggTATTCAAAGACCGAGATTAAAGAACACAGTATATACAGGGTGATTCTGCACATTACTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATCCTCATCAGAGAATTGccc                                                                                                                                            <  2:366506/150‑1 (MQ=255)
           ggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATCCTCATCAGAGAATTGCCCt                                                                                                                                           <  1:1882800/150‑1 (MQ=255)
               ttCAAAGACCGCGTTTTAAGAAGACAGTATCTACAGGGGGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTc                                                                                                                                       <  1:1146198/150‑1 (MQ=255)
               ttCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTc                                                                                                                                       <  2:331187/150‑1 (MQ=255)
                tCAAAGACCTCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATGCCTATAGGCCGAGTAAGTTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCt                                                                                                                                      <  2:812602/150‑1 (MQ=255)
                  aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTTCACTTTTCttt                                                                                                                                    >  1:256201/1‑150 (MQ=255)
                  aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                                    >  2:9343/1‑150 (MQ=255)
                  aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                                    >  2:1618910/1‑150 (MQ=255)
                    aGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAc                                                                                                                                  >  1:680476/1‑150 (MQ=255)
                     gACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAcc                                                                                                                                 <  2:1195780/150‑1 (MQ=255)
                       ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCTCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                               <  2:673881/150‑1 (MQ=255)
                       ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                               >  1:1333075/1‑150 (MQ=255)
                       ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                               <  1:2005931/150‑1 (MQ=255)
                        cgcgTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGa                                                                                                                              <  1:670948/150‑1 (MQ=255)
                         gcgTTTTAAGAACACAGTATCTACAGCGTGATTCTGCACATTCCTATAGGCCGAGTAAGCAGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGAc                                                                                                                             >  1:250658/1‑150 (MQ=255)
                            ttttAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACAtc                                                                                                                          >  1:1980694/1‑150 (MQ=255)
                             tttAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACAtct                                                                                                                         >  2:813566/1‑150 (MQ=255)
                             tttAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACAtct                                                                                                                         >  2:1392846/1‑150 (MQ=255)
                               tAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTcc                                                                                                                       >  2:587609/1‑150 (MQ=255)
                                        aGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAg                                                                                                              >  2:976212/1‑150 (MQ=255)
                                          tATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGaa                                                                                                            <  2:1518307/150‑1 (MQ=255)
                                            tCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAAtt                                                                                                          >  1:1260319/1‑150 (MQ=255)
                                             cTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTg                                                                                                         >  2:1633758/1‑150 (MQ=255)
                                             cTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTg                                                                                                         >  2:451722/1‑150 (MQ=255)
                                              tACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTgg                                                                                                        >  2:1918/1‑150 (MQ=255)
                                               aCAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGc                                                                                                       <  1:514568/150‑1 (MQ=255)
                                                 aGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGccc                                                                                                     <  2:1939989/150‑1 (MQ=255)
                                                   ggTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTa                                                                                                   >  1:1982615/1‑150 (MQ=255)
                                                      gATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCa                                                                                                >  1:559660/1‑150 (MQ=255)
                                                       aTTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCaa                                                                                               >  2:1102138/1‑150 (MQ=255)
                                                            gCACATTCCTATAGGCCGAGTAAGGTGTTCACGCGGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAAttctt                                                                                          >  1:1293670/1‑150 (MQ=255)
                                                            gCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAAttctt                                                                                          >  2:946055/1‑150 (MQ=255)
                                                               caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                       <  1:1408663/150‑1 (MQ=255)
                                                               caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                       <  2:1136298/150‑1 (MQ=255)
                                                               caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                       <  2:1071203/150‑1 (MQ=255)
                                                                 ttCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTACCCCCACAAGAATTGGCCCTACCAATTGTTCGCtt                                                                                     >  1:1745596/1‑150 (MQ=255)
                                                                 ttCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCGTTTCTTTACCAGACAGCTCCCCCCACAAGAATTGGCCCTACCAATTCGTCGCtt                                                                                     >  1:1753440/1‑150 (MQ=255)
                                                                    cTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCGATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATc                                                                                  >  2:439551/1‑150 (MQ=255)
                                                                     tataGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCt                                                                                 >  2:1598864/1‑150 (MQ=255)
                                                                     tataGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTACGCTTTTCt                                                                                 >  2:1123495/1‑150 (MQ=255)
                                                                            cGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACctct                                                                          >  2:411765/1‑150 (MQ=255)
                                                                              aGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTgg                                                                        <  2:461299/150‑1 (MQ=255)
                                                                                tAAGGTGTTCACGCCGCATCCTGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCGACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGtt                                                                      <  2:371207/150‑1 (MQ=255)
                                                                                 aaGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGACTTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTc                                                                     >  2:59253/1‑150 (MQ=255)
                                                                                   ggTGTTCTCGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcac                                                                   <  1:710555/150‑1 (MQ=255)
                                                                                    gtgtTCACGCCGCATCCGGCAAGTTAAGGCGCTCTGGATCAACAACCTAAGGGCATTTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcact                                                                  <  1:961989/150‑2 (MQ=255)
                                                                                    gtgtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcaca                                                                  <  2:550537/150‑1 (MQ=255)
                                                                                    gtgtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcaca                                                                  >  1:387870/1‑150 (MQ=255)
                                                                                      gtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAAt                                                                >  2:767751/1‑150 (MQ=255)
                                                                                        tCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAAttt                                                              >  1:544615/1‑150 (MQ=255)
                                                                                         cACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTc                                                             >  2:669645/1‑150 (MQ=255)
                                                                                           cgccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTccc                                                           >  2:1267822/1‑150 (MQ=255)
                                                                                            gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACACGAATTGGCCCTACCAATTCTTCGCGTAGCTGACCTCTGGGTCCCAATTTcccc                                                          >  2:1454863/1‑149 (MQ=255)
                                                                                            gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCa                                                          <  1:855692/150‑1 (MQ=255)
                                                                                             ccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTTATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTTTCTGACCTCTGGTTCACAATTTCCCaa                                                         <  1:911865/150‑1 (MQ=255)
                                                                                              cgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAAt                                                        <  2:1991441/150‑1 (MQ=255)
                                                                                                 aTCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCTACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaa                                                     <  1:1319607/150‑1 (MQ=255)
                                                                                                  tCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaa                                                    <  2:1333075/150‑1 (MQ=255)
                                                                                                   ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                                   <  2:1063802/150‑1 (MQ=255)
                                                                                                   ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                                   >  2:188800/1‑150 (MQ=255)
                                                                                                   ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                                   >  2:1838168/1‑150 (MQ=255)
                                                                                                    cGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCAGACCTCTGGTTCAAAATTACACAATTaaaaa                                                  >  2:904025/1‑149 (MQ=255)
                                                                                                     ggCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACt                                                 <  1:579998/150‑1 (MQ=255)
                                                                                                          gATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACAt                                            >  2:1778933/1‑150 (MQ=255)
                                                                                                            tAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCa                                          <  2:922604/150‑1 (MQ=255)
                                                                                                               ggCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATg                                       >  2:1339018/1‑150 (MQ=255)
                                                                                                                    tctGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGcc                                  <  1:1145595/150‑1 (MQ=255)
                                                                                                                       ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGCCCGCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                               >  1:1235272/1‑150 (MQ=255)
                                                                                                                       ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                               >  2:1733146/1‑150 (MQ=255)
                                                                                                                       ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                               >  1:184684/1‑150 (MQ=255)
                                                                                                                        gATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATa                              <  1:1423138/150‑1 (MQ=255)
                                                                                                                         aTCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATAc                             >  2:857554/1‑150 (MQ=255)
                                                                                                                           caacaaCCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAt                           <  2:997546/150‑1 (MQ=255)
                                                                                                                           caacaaCCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACATCTGGTTAACAATTTCCCAAATAAAACTAACAAAAAAGTTGCCAATACAt                           >  1:374365/1‑150 (MQ=255)
                                                                                                                              caacCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAAc                        <  2:680476/150‑1 (MQ=255)
                                                                                                                                  cTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTa                   <  1:1430806/150‑1 (MQ=255)
                                                                                                                                   tAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTa                   <  1:1788812/150‑1 (MQ=255)
                                                                                                                                   tAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAAAACATTTa                   <  1:1363358/150‑1 (MQ=255)
                                                                                                                                   aaaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTATTCGCTTATCTGACATCTGGTTCACAATTTCCCAATTAAAACTAACATCAATGATGCCACTACATAACATTTa                   >  1:954107/2‑150 (MQ=255)
                                                                                                                                    aaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAg                  >  2:693857/1‑150 (MQ=255)
                                                                                                                                     aGGGCATTTCTCTGATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCACACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt                 <  1:159334/150‑1 (MQ=255)
                                                                                                                                     aGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt                 <  1:1392846/150‑1 (MQ=255)
                                                                                                                                      gggCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGTCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGtt                <  2:1980694/150‑1 (MQ=255)
                                                                                                                                           aTTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAAAca           >  1:861911/1‑150 (MQ=255)
                                                                                                                                             tctcTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCAGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcatt         >  1:1353926/1‑150 (MQ=255)
                                                                                                                                               tctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcattca       <  1:1267822/150‑1 (MQ=255)
                                                                                                                                               tctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCAAAAGAATTGGCCCTACCAATTCTTCGCTTATCTTACATCTGTTTCACAATATCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcattca       <  1:1064510/150‑1 (MQ=255)
                                                                                                                                                ctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcattcat      >  2:234023/1‑150 (MQ=255)
                                                                                                                                                  gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTTTCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACCTCAATGTTGCCAATACATAACATTTAGTTAACCCTTCATTg    >  1:1528698/1‑150 (MQ=255)
                                                                                                                                                  gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTg    >  1:520755/1‑150 (MQ=255)
                                                                                                                                                  gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTg    <  2:892653/150‑1 (MQ=255)
                                                                                                                                                  gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTACCAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAAAACATTTAGTTAACcattcattt    >  2:1091404/1‑149 (MQ=255)
                                                                                                                                                    tgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTc  <  2:303345/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGAGATGACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTC  >  NC_000913/3776994‑3777291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: