Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,777,146 G→A 5.9% intergenic (+119/‑253) yibL → / → lldP ribosome‑associated DUF2810 family protein/L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,777,1460GA 5.9% 423.6 / 9.0 134intergenic (+119/‑253)yibL/lldPribosome‑associated DUF2810 family protein/L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base G (70/56);  new base A (6/2);  total (76/58)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 134 total aligned reads displayed.

ACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTCAT  >  NC_000913/3777001‑3777293
                                                                                                                                                 |                                                                                                                                                   
aCTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTg                                                                                                                                                 >  2:1423138/1‑150 (MQ=255)
  tGGGTTTTCAATGACCGCGTTTTAAGAACTCAGTATCTACAGGGTGATTCTGCACATTCCTTTCGGCCGAGTAAGGTGTTGTCGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGcc                                                                                                                                               <  2:765323/150‑1 (MQ=255)
   gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                              <  2:1718701/150‑1 (MQ=255)
   gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                              <  2:1729005/150‑1 (MQ=255)
   gggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGccc                                                                                                                                              <  1:739115/150‑1 (MQ=255)
   gggTATTCAAAGACCGAGATTAAAGAACACAGTATATACAGGGTGATTCTGCACATTACTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATCCTCATCAGAGAATTGccc                                                                                                                                              <  2:366506/150‑1 (MQ=255)
    ggTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATCCTCATCAGAGAATTGCCCt                                                                                                                                             <  1:1882800/150‑1 (MQ=255)
        ttCAAAGACCGCGTTTTAAGAAGACAGTATCTACAGGGGGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTc                                                                                                                                         <  1:1146198/150‑1 (MQ=255)
         tCAAAGACCTCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATGCCTATAGGCCGAGTAAGTTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCt                                                                                                                                        <  2:812602/150‑1 (MQ=255)
           aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                                      >  2:9343/1‑150 (MQ=255)
           aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                                      >  1:550545/1‑150 (MQ=255)
           aaaGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCttt                                                                                                                                      >  2:1618910/1‑150 (MQ=255)
             aGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAc                                                                                                                                    >  1:680476/1‑150 (MQ=255)
              gACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTAcc                                                                                                                                   <  2:1195780/150‑1 (MQ=255)
                ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCTCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                                 <  2:673881/150‑1 (MQ=255)
                ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                                 >  1:1333075/1‑150 (MQ=255)
                ccGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAg                                                                                                                                 <  1:2005931/150‑1 (MQ=255)
                 cgcgTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGa                                                                                                                                <  1:670948/150‑1 (MQ=255)
                  gcgTTTTAAGAACACAGTATCTACAGCGTGATTCTGCACATTCCTATAGGCCGAGTAAGCAGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGAc                                                                                                                               >  1:250658/1‑150 (MQ=255)
                     ttttAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACAtc                                                                                                                            >  1:1980694/1‑150 (MQ=255)
                      tttAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACAtct                                                                                                                           >  2:813566/1‑150 (MQ=255)
                        tAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTcc                                                                                                                         >  2:587609/1‑150 (MQ=255)
                                 aGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAg                                                                                                                >  2:976212/1‑150 (MQ=255)
                                     tCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAAtt                                                                                                            >  1:1260319/1‑150 (MQ=255)
                                      cTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTg                                                                                                           >  2:451722/1‑150 (MQ=255)
                                        aCAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGc                                                                                                         <  1:514568/150‑1 (MQ=255)
                                         cAGGGTGATTCAGCAAATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGcc                                                                                                        <  1:269509/150‑1 (MQ=255)
                                          aGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGccc                                                                                                       <  2:1939989/150‑1 (MQ=255)
                                           gggTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCt                                                                                                      >  2:621066/1‑150 (MQ=255)
                                               gATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCa                                                                                                  >  1:559660/1‑150 (MQ=255)
                                                aTTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCaa                                                                                                 >  2:1102138/1‑150 (MQ=255)
                                                     gCACATTCCTATAGGCCGAGTAAGGTGTTCACGCGGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAAttctt                                                                                            >  1:1293670/1‑150 (MQ=255)
                                                     gCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAAttctt                                                                                            >  2:946055/1‑150 (MQ=255)
                                                        caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                         <  2:1136298/150‑1 (MQ=255)
                                                        caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                         <  2:1071203/150‑1 (MQ=255)
                                                        caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                         >  2:1788812/1‑150 (MQ=255)
                                                        caTTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGc                                                                                         <  1:1408663/150‑1 (MQ=255)
                                                          ttCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTACCCCCACAAGAATTGGCCCTACCAATTGTTCGCtt                                                                                       >  1:1745596/1‑150 (MQ=255)
                                                          ttCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCGTTTCTTTACCAGACAGCTCCCCCCACAAGAATTGGCCCTACCAATTCGTCGCtt                                                                                       >  1:1753440/1‑150 (MQ=255)
                                                             cTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCGATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATc                                                                                    >  2:439551/1‑150 (MQ=255)
                                                              tataGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCt                                                                                   >  2:1598864/1‑150 (MQ=255)
                                                              tataGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTACGCTTTTCt                                                                                   >  2:1123495/1‑150 (MQ=255)
                                                                         tAAGGTGTTCACGCCGCATCCTGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCGACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGtt                                                                        <  2:371207/150‑1 (MQ=255)
                                                                          aatgtgtTCACGCCGCATACGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAAATCTCTTATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTc                                                                       <  2:769033/147‑1 (MQ=255)
                                                                            ggTGTTCTCGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcac                                                                     <  1:710555/150‑1 (MQ=255)
                                                                            ggTGTTCACGCCGCATCCGCCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAAATCTCTGAAGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGACAAACCAATTCTTCGCTTATAAGACCTCTGGTTcaa                                                                     >  2:339017/1‑149 (MQ=255)
                                                                             gtgtTCACGCCGCATCCGGCAAGTTAAGGCGCTCTGGATCAACAACCTAAGGGCATTTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcact                                                                    <  1:961989/150‑2 (MQ=255)
                                                                             gtgtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcaca                                                                    <  2:497315/150‑1 (MQ=255)
                                                                             gtgtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcaca                                                                    <  2:550537/150‑1 (MQ=255)
                                                                             gtgtTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTcaca                                                                    >  1:387870/1‑150 (MQ=255)
                                                                                  cACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTc                                                               >  2:669645/1‑150 (MQ=255)
                                                                                    cgccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTccc                                                             >  2:426748/1‑150 (MQ=255)
                                                                                     gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACACGAATTGGCCCTACCAATTCTTCGCGTAGCTGACCTCTGGGTCCCAATTTcccc                                                            >  2:1454863/1‑149 (MQ=255)
                                                                                     gccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCa                                                            <  1:855692/150‑1 (MQ=255)
                                                                                      ccgcATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTTATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTTTCTGACCTCTGGTTCACAATTTCCCaa                                                           <  1:911865/150‑1 (MQ=255)
                                                                                          aTCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCTACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaa                                                       <  1:1319607/150‑1 (MQ=255)
                                                                                           tCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaa                                                      <  2:1333075/150‑1 (MQ=255)
                                                                                            ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                                     <  2:1063802/150‑1 (MQ=255)
                                                                                            ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                                     >  2:188800/1‑150 (MQ=255)
                                                                                            ccGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTaaaa                                                     >  2:1838168/1‑150 (MQ=255)
                                                                                             cGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCAGACCTCTGGTTCAAAATTACACAATTaaaaa                                                    >  2:904025/1‑149 (MQ=255)
                                                                                              ggCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACt                                                   <  1:579998/150‑1 (MQ=255)
                                                                                                     tAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCa                                            <  2:922604/150‑1 (MQ=255)
                                                                                                        ggCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATg                                         >  2:1339018/1‑150 (MQ=255)
                                                                                                             tctGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGcc                                    <  1:1145595/150‑1 (MQ=255)
                                                                                                                ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGCCCGCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                                 >  1:1235272/1‑150 (MQ=255)
                                                                                                                ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                                 >  2:1733146/1‑150 (MQ=255)
                                                                                                                ggATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAAt                                 >  1:184684/1‑150 (MQ=255)
                                                                                                                 gATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATa                                <  1:1423138/150‑1 (MQ=255)
                                                                                                                  aTCAACACCCTCACGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATAc                               <  2:1923557/150‑1 (MQ=255)
                                                                                                                  aTCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATAc                               >  2:857554/1‑150 (MQ=255)
                                                                                                                   tCAACAACCTAATGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACa                              <  1:2004208/150‑1 (MQ=255)
                                                                                                                   tCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACa                              >  2:213688/1‑150 (MQ=255)
                                                                                                                    caacaaCCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAt                             <  2:997546/150‑1 (MQ=255)
                                                                                                                    caacaaCCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACATCTGGTTAACAATTTCCCAAATAAAACTAACAAAAAAGTTGCCAATACAt                             >  1:374365/1‑150 (MQ=255)
                                                                                                                      acaacCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATaa                           >  1:476929/1‑150 (MQ=255)
                                                                                                                           cTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTa                     <  1:1430806/150‑1 (MQ=255)
                                                                                                                            tAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTa                     <  1:1788812/150‑1 (MQ=255)
                                                                                                                            tAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAAAACATTTa                     <  1:1363358/150‑1 (MQ=255)
                                                                                                                            aaaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTa                     >  2:686343/2‑150 (MQ=255)
                                                                                                                            aaaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTATTCGCTTATCTGACATCTGGTTCACAATTTCCCAATTAAAACTAACATCAATGATGCCACTACATAACATTTa                     >  1:954107/2‑150 (MQ=255)
                                                                                                                             aaGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAg                    >  2:693857/1‑150 (MQ=255)
                                                                                                                              aGGGCATTTCTCTGATGAGGATTGACCTTTTCTTTACCAGACATCTCCCCACACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt                   <  1:159334/150‑1 (MQ=255)
                                                                                                                              aGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt                   <  1:1392846/150‑1 (MQ=255)
                                                                                                                              aGGGCAATCCTCATCAGAGAATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGt                   >  2:152976/1‑150 (MQ=255)
                                                                                                                               gggCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGTCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGtt                  <  2:1980694/150‑1 (MQ=255)
                                                                                                                                    aTTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAAAca             >  1:861911/1‑150 (MQ=255)
                                                                                                                                      tctcTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCAGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcatt           >  1:1353926/1‑150 (MQ=255)
                                                                                                                                        tctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcattca         <  1:1267822/150‑1 (MQ=255)
                                                                                                                                        tctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCAAAAGAATTGGCCCTACCAATTCTTCGCTTATCTTACATCTGTTTCACAATATCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcattca         <  1:1064510/150‑1 (MQ=255)
                                                                                                                                         ctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGc                                     <  2:129169/121‑1 (MQ=255)
                                                                                                                                         ctGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACcattcat        >  2:234023/1‑150 (MQ=255)
                                                                                                                                           gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTTTCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACCTCAATGTTGCCAATACATAACATTTAGTTAACCCTTCATTg      >  1:1528698/1‑150 (MQ=255)
                                                                                                                                           gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTg      >  1:520755/1‑150 (MQ=255)
                                                                                                                                           gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTg      <  2:892653/150‑1 (MQ=255)
                                                                                                                                           gatgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTACCAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACAAAACATTTAGTTAACcattcattt      >  2:1091404/1‑149 (MQ=255)
                                                                                                                                             tgaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTc    <  2:303345/150‑1 (MQ=255)
                                                                                                                                              gaGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTCa   <  2:1260319/150‑1 (MQ=255)
                                                                                                                                               aGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGGCAt  <  1:188800/150‑1 (MQ=255)
                                                                                                                                               aGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATATGACCTCTGGTTAACAATTTACCAATTAAAACTCACATCAATGGTGCCAATACATAACATTTAGTTAACCATTAATTGTAAt  >  2:1480735/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACTGGGTTTTCAAAGACCGCGTTTTAAGAACACAGTATCTACAGGGTGATTCTGCACATTCCTATAGGCCGAGTAAGGTGTTCACGCCGCATCCGGCAAGATAAGGCGCTCTGGATCAACAACCTAAGGGCAATTCTCTGATGAGGATTGCCCTTTTCTTTACCAGACATCTCCCCCCACAAGAATTGGCCCTACCAATTCTTCGCTTATCTGACCTCTGGTTCACAATTTCCCAATTAAAACTCACATCAATGTTGCCAATACATAACATTTAGTTAACCATTCATTGTCAT  >  NC_000913/3777001‑3777293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: