Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,853,674 G→T 5.4% intergenic (+32/‑248) tisB → / → emrD toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,853,6740GT 5.4% 520.1 / 10.6 162intergenic (+32/‑248)tisB/emrDtoxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (83/70);  new base T (6/3);  total (89/73)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 166 total aligned reads displayed.

CAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTA  >  NC_000913/3853525‑3853822
                                                                                                                                                     |                                                                                                                                                     
 aGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGa                                                                                                                                                      <  1:1075376/150‑1 (MQ=255)
     cTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAAcc                                                                                                                                                  <  2:1076183/150‑1 (MQ=255)
     cTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAAcc                                                                                                                                                  <  1:213323/150‑1 (MQ=255)
       ccTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGc                                                                                                                                                >  1:980430/1‑150 (MQ=255)
       ccTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGc                                                                                                                                                <  1:1111314/150‑1 (MQ=255)
           aaCCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGTTTCCCGCTccc                                                                                                                                            <  2:1715059/150‑1 (MQ=255)
             ccACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCt                                                                                                                                         <  1:251030/150‑1 (MQ=255)
               acaGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGAAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGTTTCCCGCTCCCCttt                                                                                                                                       <  1:1611793/150‑1 (MQ=255)
                caGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCtttt                                                                                                                                      <  1:1344797/150‑1 (MQ=255)
                  ggAGACGCGGATGAACGTGGGGGCTATCTCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTTATGCTGGTCTGAAATACCTGAACTAATTCAGATTCAAGTCGCACCAAAGTGGAGCGGGAAACCGCTCCCCTTTtat                                                                                                                                    <  1:1509180/150‑1 (MQ=255)
                        gcgTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAg                                                                                                                              <  2:1149110/150‑1 (MQ=255)
                           tATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTtatatatatagt                                                                                                                           >  1:1513455/1‑146 (MQ=255)
                            aTGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTg                                                                                                                          >  2:1312229/1‑150 (MQ=255)
                             tGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGc                                                                                                                         <  2:429963/150‑1 (MQ=255)
                               aaCCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTATCGTGCgg                                                                                                                       >  1:1750724/1‑150 (MQ=255)
                                aCCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCGCCCCTTTTATATTTAGCGTGCggg                                                                                                                      <  1:927007/150‑1 (MQ=255)
                                  cTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGtt                                                                                                                    >  2:1019712/1‑150 (MQ=255)
                                       ggATATCGCAATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGgtgt                                                                                                               <  1:1168285/150‑1 (MQ=255)
                                         atatCGCCTTTCTTATCCTCAAACTCATTTTTGCAGCACTGTAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCg                                                                                                             <  2:764394/150‑1 (MQ=255)
                                         atatCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCg                                                                                                             <  1:1247316/150‑1 (MQ=255)
                                           atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTTATATTTAGCGTGCGGGTTGGTGTCgg                                                                                                            >  1:375724/1‑150 (MQ=255)
                                           atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGga                                                                                                           <  1:406568/150‑1 (MQ=255)
                                           atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGga                                                                                                           <  2:1843199/150‑1 (MQ=255)
                                           atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGga                                                                                                           >  1:1229701/1‑150 (MQ=255)
                                              gCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatgc                                                                                                        >  2:1074686/1‑150 (MQ=255)
                                               ccATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatgcg                                                                                                       >  2:819211/1‑150 (MQ=255)
                                                cATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatgcga                                                                                                      <  1:1357067/150‑1 (MQ=255)
                                                    cTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCt                                                                                                  >  2:1299360/1‑150 (MQ=255)
                                                    cTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCt                                                                                                  >  1:713772/1‑150 (MQ=255)
                                                         ccTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGc                                                                                             >  1:1983444/1‑150 (MQ=255)
                                                          cTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCa                                                                                            >  1:1703475/1‑150 (MQ=255)
                                                          cTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCa                                                                                            >  2:1978488/1‑150 (MQ=255)
                                                          atCAAACTCATTGTTGCAGCACTGCAACTGCTGGATGCTGTTCTGAAATACATGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACAGCTCCCCTTTTATATTTAGCGTGCGGGTTTGTTTCGTATGCGATGATGAAGCa                                                                                            >  2:1385495/2‑150 (MQ=255)
                                                             aaaCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAGTACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCt                                                                                         <  2:1987783/150‑1 (MQ=255)
                                                             aaaCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAATTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGAGATGCTGACGCATCt                                                                                         >  2:685065/1‑150 (MQ=255)
                                                             aaaCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCAtat                                                                                         <  1:107012/150‑3 (MQ=255)
                                                              aaCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCtt                                                                                        <  1:1604479/150‑1 (MQ=255)
                                                               aCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAACGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTa                                                                                       <  2:116730/150‑1 (MQ=255)
                                                                cTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTAt                                                                                      >  1:1026313/1‑150 (MQ=255)
                                                                 tCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCACAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATc                                                                                     <  1:1315429/150‑1 (MQ=255)
                                                                  cATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATcc                                                                                    >  2:608750/1‑150 (MQ=255)
                                                                  cATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATcc                                                                                    >  2:1821458/1‑150 (MQ=255)
                                                                  cATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATcc                                                                                    >  1:950680/1‑150 (MQ=255)
                                                                        tgCAGCACTGCAACTGCTTGCTGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATAATTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCAGCCCTa                                                                              >  1:81117/1‑150 (MQ=255)
                                                                         gcagcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTAc                                                                             >  1:469835/1‑150 (MQ=255)
                                                                         gcagcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTAc                                                                             >  1:1703174/1‑150 (MQ=255)
                                                                          cagcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCTGATGCGAAGATGACGCATCTTATCCGCCCtcac                                                                            >  2:1240377/1‑147 (MQ=255)
                                                                          cagcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTAcc                                                                            >  2:873994/1‑150 (MQ=255)
                                                                             caCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCAtc                                                                         >  1:1644728/1‑150 (MQ=255)
                                                                              aCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCAtc                                                                         <  2:375724/150‑1 (MQ=255)
                                                                                tGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGCCGCAGCTTATCCGCCCTACCAtctct                                                                      >  2:1278288/1‑150 (MQ=255)
                                                                                  cAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTcc                                                                    >  2:1078169/1‑150 (MQ=255)
                                                                                  cAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGATGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTcc                                                                    >  2:35222/1‑150 (MQ=255)
                                                                                   aaCTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTccc                                                                   <  2:636108/150‑1 (MQ=255)
                                                                                             tGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACAttt                                                         >  1:1202971/1‑150 (MQ=255)
                                                                                                tGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTAtt                                                      <  2:1403785/150‑1 (MQ=255)
                                                                                                  ttCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGc                                                    <  1:715630/150‑1 (MQ=255)
                                                                                                  ttCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGc                                                    >  2:1570231/1‑150 (MQ=255)
                                                                                                  ttCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGc                                                    <  2:153413/150‑1 (MQ=255)
                                                                                                  ttCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGGGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGc                                                    <  1:1177770/150‑1 (MQ=255)
                                                                                                    cTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCg                                                  <  1:903805/150‑1 (MQ=255)
                                                                                                    cTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCg                                                  <  1:895960/150‑1 (MQ=255)
                                                                                                          tACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTg                                            >  1:273589/1‑150 (MQ=255)
                                                                                                            ccTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGtt                                          <  2:1369002/150‑1 (MQ=255)
                                                                                                            ccTGAAGAAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCAGTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCGGGCAACATTTATTGCCGCTTTTGtt                                          <  2:382302/150‑1 (MQ=255)
                                                                                                               gAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTAc                                       >  2:1509027/1‑150 (MQ=255)
                                                                                                                 aGTAATTCAGATTCAAGACGCACCAAAGGGGAGCGGGAAACCGCTCCCCATTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGTTGCTGACGCAACTTATACGCCCAACCAACTCTCCCGGCAACAATTATTCGCGCGATTCTTTACat                                     >  1:2014650/1‑150 (MQ=255)
                                                                                                                     aTTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTc                                 >  2:121888/1‑150 (MQ=255)
                                                                                                                      ttCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCt                                >  1:75654/1‑150 (MQ=255)
                                                                                                                      ttCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCt                                >  2:2014771/1‑150 (MQ=255)
                                                                                                                      ttCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCt                                >  2:467715/1‑150 (MQ=255)
                                                                                                                        cAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGc                              <  2:383731/150‑1 (MQ=255)
                                                                                                                         aGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGcc                             <  1:1213321/150‑1 (MQ=255)
                                                                                                                         aGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGcc                             >  1:669942/1‑150 (MQ=255)
                                                                                                                           aTTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGc                           <  1:518075/150‑1 (MQ=255)
                                                                                                                              cAAGTCGCACCATAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTTTTACATATTCTGCCGCTaaa                       <  2:587138/150‑1 (MQ=255)
                                                                                                                              cAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATAGTCTGCCGCTaa                        >  1:18805/1‑150 (MQ=255)
                                                                                                                               aaGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTaaa                       >  2:1115626/1‑150 (MQ=255)
                                                                                                                                 gTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACa                     >  1:1091823/1‑150 (MQ=255)
                                                                                                                                 gTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATACGCCCTACCATCTCTCCCGGCAAAATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACa                     >  2:54717/1‑150 (MQ=255)
                                                                                                                                  tCGCACCAAAGGGGAGCGGGAAACAGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGTATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACaa                    <  2:630672/150‑1 (MQ=255)
                                                                                                                                    gCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAAtt                  >  1:1420974/1‑150 (MQ=255)
                                                                                                                                    gCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAAtt                  >  1:195759/1‑150 (MQ=255)
                                                                                                                                     cACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTc                 <  2:489289/150‑1 (MQ=255)
                                                                                                                                       ccAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTccc               >  1:1556365/1‑150 (MQ=255)
                                                                                                                                       ccAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTccc               >  1:1427287/1‑150 (MQ=255)
                                                                                                                                        cAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTcccc              >  2:1883674/1‑150 (MQ=255)
                                                                                                                                        cAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTcccc              >  1:428529/1‑150 (MQ=255)
                                                                                                                                          aaGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCAt            >  2:2001386/1‑150 (MQ=255)
                                                                                                                                           aGGGGAGCGGTTTCCCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCAtt           >  1:58335/1‑150 (MQ=255)
                                                                                                                                           aGGGGAGCGGTTTCCCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCAtt           >  1:1172827/1‑150 (MQ=255)
                                                                                                                                            ggggAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTc          >  2:1581663/1‑150 (MQ=255)
                                                                                                                                             gggAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTcc         <  2:1732401/150‑1 (MQ=255)
                                                                                                                                               gAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTg       <  2:1229701/150‑1 (MQ=255)
                                                                                                                                                 gCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGc     >  1:1476610/1‑150 (MQ=255)
                                                                                                                                                  cGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCg    <  1:584907/150‑1 (MQ=255)
                                                                                                                                                  cGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCg    <  1:1012928/150‑1 (MQ=255)
                                                                                                                                                   gggAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCACCATTCCTGGCGt   >  2:823690/1‑150 (MQ=255)
                                                                                                                                                   gggAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATATCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGt   >  2:1434033/1‑150 (MQ=255)
                                                                                                                                                    ggAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGta  <  1:1012715/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTA  >  NC_000913/3853525‑3853822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: