Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,906,828 A→T 5.6% intergenic (‑261/+25) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,906,8280AT 5.6% 410.1 / 8.5 152intergenic (‑261/+25)bglG/phoUtranscriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
Reads supporting (aligned to +/- strand):  ref base A (67/76);  new base T (4/5);  total (71/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 155 total aligned reads displayed.

ATAACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAA  >  NC_000913/3906679‑3906974
                                                                                                                                                     |                                                                                                                                                  
ataACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCTCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTa                                                                                                                                                    <  2:146562/150‑1 (MQ=255)
 taACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTaa                                                                                                                                                   >  1:843677/1‑150 (MQ=255)
    cGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGAGCTATTGATAAAAATATGACCATGCACGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATAAAGtt                                                                                                                                                >  1:1606697/1‑150 (MQ=255)
      aaCATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAt                                                                                                                                              >  1:1378470/1‑150 (MQ=255)
         atacAGGTTCTGAAATTAATTTAATTTAGTGCAGAGAAAATAGAAATGCGCTATTGATAAAAATATGAACATTCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTgg                                                                                                                                           <  1:124895/147‑1 (MQ=255)
          tCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTGGGAATTTATAAAGTTAAATATAACAAATCCCAAAAATTAAGTTATTggt                                                                                                                                          >  2:275658/1‑149 (MQ=255)
           ccAGTTTCGGAAATTAATTTAATTGCGTGAAGAGAAAATAGCAATGAGCTATTGATAAAAATATTACCATGCTCGCAGTTATTAACTTTTTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGa                                                                                                                                         <  2:329570/150‑1 (MQ=255)
           ccAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGa                                                                                                                                         <  1:1343009/150‑1 (MQ=255)
            cAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAt                                                                                                                                        >  1:870940/1‑150 (MQ=255)
               gTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTg                                                                                                                                     <  1:1549524/150‑1 (MQ=255)
               gTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTg                                                                                                                                     <  2:717154/150‑1 (MQ=255)
               gTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTg                                                                                                                                     <  2:1985764/150‑1 (MQ=255)
               gTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGATTTg                                                                                                                                     <  2:2007737/150‑1 (MQ=255)
               gTTAGGAAATTAATTTAATTTCGTGAATAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTg                                                                                                                                     <  1:335069/150‑1 (MQ=255)
                ttCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGt                                                                                                                                    >  1:950379/1‑150 (MQ=255)
                   tgAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTTTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTg                                                                                                                                 <  2:443910/149‑1 (MQ=255)
                   ggAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTg                                                                                                                                 >  2:570084/1‑150 (MQ=255)
                    gAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTgg                                                                                                                                >  1:13419/1‑150 (MQ=255)
                     aaaTTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGGTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGt                                                                                                                               >  2:50564/1‑150 (MQ=255)
                      aattaattTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTg                                                                                                                              <  2:1526405/150‑1 (MQ=255)
                      aattaattTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTg                                                                                                                              <  2:1600870/150‑1 (MQ=255)
                         taatttaattGCGTGCAGAGAAAATAGAAATGCGCTATTGATAAAAATAAGACCATGCTCTCAGTTATAAACTTTGTGTAATTTTAGGACTTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAat                                                                                                                           <  1:72829/150‑1 (MQ=255)
                          aatttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatt                                                                                                                          <  1:1141061/150‑1 (MQ=255)
                             ttaattGCGTGCAGTGAAAATAGCAATGCTCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattatt                                                                                                                       <  2:1207308/150‑1 (MQ=255)
                             ttaattGCGTGCAGAGAAAATAGGAATTCGCTATTGATAAAAATATGACCATGCTCTCAGTTATTGACTTTGTGTTCTTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATTATTAAGTTATTGGGATTTGTCTGGTGAattatt                                                                                                                       <  1:283397/150‑1 (MQ=255)
                             ttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattatt                                                                                                                       <  1:643233/150‑1 (MQ=255)
                              taattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATATTTAAGTTATTGGGATTTGTATGGTGAATTAttt                                                                                                                      >  1:157974/1‑150 (MQ=255)
                              taattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTAttt                                                                                                                      <  1:1251433/150‑1 (MQ=255)
                                  tGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTca                                                                                                                  >  1:212031/1‑149 (MQ=255)
                                  tGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCg                                                                                                                  <  2:1702825/150‑1 (MQ=255)
                                  tGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCg                                                                                                                  <  2:551638/150‑1 (MQ=255)
                                   gCGTGCAGAGAAAATAGCAATTCGCTATTGATAAAAATATGACCATGCTCGCAGTTATAAACTTTGTGTAATTTTATGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGc                                                                                                                 <  1:326227/150‑1 (MQ=255)
                                     gTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTa                                                                                                               <  1:1145448/150‑1 (MQ=255)
                                       gCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATc                                                                                                             >  2:1292640/1‑150 (MQ=255)
                                         agagAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCtt                                                                                                           >  1:423917/1‑150 (MQ=255)
                                          gagaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCttt                                                                                                          >  2:568445/1‑150 (MQ=255)
                                          gagaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCttt                                                                                                          >  2:1931669/1‑150 (MQ=255)
                                           agaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTc                                                                                                         <  1:1359456/150‑1 (MQ=255)
                                             aaaaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                       <  2:2011637/150‑1 (MQ=255)
                                                 tAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGcc                                                                                                   <  1:927020/150‑1 (MQ=255)
                                                 tAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGcc                                                                                                   <  1:394377/150‑1 (MQ=255)
                                                  aGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCca                                                                                                  <  2:985931/150‑1 (MQ=255)
                                                      aTGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCcagcat                                                                                              >  2:929852/1‑149 (MQ=255)
                                                        gcgcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGtt                                                                                            <  1:251731/150‑1 (MQ=255)
                                                          gcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                          <  2:1509652/150‑1 (MQ=255)
                                                          gcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                          <  1:462112/150‑1 (MQ=255)
                                                          gcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                          <  2:843677/150‑1 (MQ=255)
                                                           cTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATTATTAAGTTATTGGGATTTGTCTGTTGAATTATTTGTCGCTATCTTTCCACTCAAGCAGTTTAt                                                                                         >  1:357293/1‑150 (MQ=255)
                                                                gATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCTCTATCTTTCACCGCCAGCAGTTTATCCAGc                                                                                    >  1:191015/1‑150 (MQ=255)
                                                                gATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                    >  2:1641541/1‑150 (MQ=255)
                                                                 aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCt                                                                                   >  2:653345/1‑150 (MQ=255)
                                                                   aaaaaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTNAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCa                                                                                 <  1:622111/150‑1 (MQ=255)
                                                                   aaaaaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCa                                                                                 <  1:630427/150‑1 (MQ=255)
                                                                     aaaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATc                                                                               <  1:486782/150‑1 (MQ=255)
                                                                      aaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCg                                                                              <  2:1970847/150‑1 (MQ=255)
                                                                      aaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCg                                                                              >  1:407307/1‑150 (MQ=255)
                                                                         atGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCa                                                                           >  1:577933/1‑150 (MQ=255)
                                                                             ccATGCTCGCAGTTATTAACTTTGTGTTATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCTCCACCGa                                                                       >  2:55446/1‑150 (MQ=255)
                                                                             ccATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGa                                                                       >  2:1563290/1‑150 (MQ=255)
                                                                             ccATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGa                                                                       >  2:1972074/1‑150 (MQ=255)
                                                                                   tCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGATATTGGGATTTGTCTGGTGAATTATTAGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGAc                                                                 <  1:296218/150‑1 (MQ=255)
                                                                                     gCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACgg                                                               <  1:960664/150‑1 (MQ=255)
                                                                                          tattaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATc                                                          >  1:1045628/1‑150 (MQ=255)
                                                                                          tattaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATc                                                          >  1:767824/1‑150 (MQ=255)
                                                                                           attaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATcc                                                         >  2:1955528/1‑150 (MQ=255)
                                                                                              aaCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGATCATCGCCACCGACGTGACGGAAATCCTGc                                                      <  2:1999665/150‑1 (MQ=255)
                                                                                               aCTTTGTGTAATTTTAGGAATTTATAAAGTTTTATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcc                                                     <  1:782573/150‑1 (MQ=255)
                                                                                                 tttGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcccc                                                   <  1:416403/150‑1 (MQ=255)
                                                                                                    gtttAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTc                                                <  2:1041432/147‑1 (MQ=255)
                                                                                                    gtgtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTc                                                <  1:568445/150‑1 (MQ=255)
                                                                                                       tAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCATCTAATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACg                                             >  2:1394072/1‑150 (MQ=255)
                                                                                                          ttttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCTCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtag                                          >  2:1431276/1‑150 (MQ=255)
                                                                                                          ttttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCTCCACCGACGTGACGGAAATCCTGCCCCTTCAAgtag                                          >  2:1257506/1‑150 (MQ=255)
                                                                                                             tAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCGCCTTCACGtagtag                                       <  2:1126661/150‑1 (MQ=255)
                                                                                                             tAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGtagtag                                       >  2:1675507/1‑150 (MQ=255)
                                                                                                               ggAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaa                                     >  1:1743539/1‑150 (MQ=255)
                                                                                                                 aaTTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaaga                                   >  2:1056349/1‑150 (MQ=255)
                                                                                                                  aTTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGAt                                  <  2:789562/150‑1 (MQ=255)
                                                                                                                     tataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATaaa                               <  2:874034/150‑1 (MQ=255)
                                                                                                                     tataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATaaa                               >  1:1963130/1‑150 (MQ=255)
                                                                                                                       taAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACt                             >  2:1339494/1‑150 (MQ=255)
                                                                                                                        aaaGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTc                            <  2:1894291/150‑1 (MQ=255)
                                                                                                                         aaGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTca                           <  2:870940/150‑1 (MQ=255)
                                                                                                                             tatatataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACaaa                       <  2:1378470/150‑1 (MQ=255)
                                                                                                                                atataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAGTTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAatat                    >  2:1086938/1‑150 (MQ=255)
                                                                                                                                atataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAatat                    >  2:915544/1‑150 (MQ=255)
                                                                                                                                  ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc                  <  1:1013084/150‑1 (MQ=255)
                                                                                                                                   taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCAGCCAGAAGTTTATACAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTCGAAGATAAACTCACAAATATTCt                 >  2:828944/1‑150 (MQ=255)
                                                                                                                                    aaCAAATCCCAATAATTAAGTTATTGTGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                <  2:157974/150‑1 (MQ=255)
                                                                                                                                     aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTgg               <  1:275658/150‑1 (MQ=255)
                                                                                                                                     aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTgg               <  2:1542697/150‑1 (MQ=255)
                                                                                                                                      cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGTTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATACAGCTCATCGCCACCGACGTGACGGAAATCCTTCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGc              <  1:1606167/150‑1 (MQ=255)
                                                                                                                                        aaTCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCACCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg            >  2:467935/1‑150 (MQ=255)
                                                                                                                                          tCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCg          >  1:823922/1‑150 (MQ=255)
                                                                                                                                           cccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCgg         <  1:1391214/150‑1 (MQ=255)
                                                                                                                                            ccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGt        >  1:1627265/1‑150 (MQ=255)
                                                                                                                                             cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTc       >  2:657230/1‑150 (MQ=255)
                                                                                                                                                taatTATGTTTTTGGGTTTTGTCTGGTGTATTATTTGTCGCTATCTTTCCCCGACAGCAGTTTATCCAGCTGATCGCAACCGACGTGACTGAAATCCTGCCCCTTCACGTAGTAGAATATAAACTCTCAAATATTCTGGCAGCGGTCGcc    <  2:690818/150‑1 (MQ=255)
                                                                                                                                                taatTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGcc    <  2:1607303/150‑1 (MQ=255)
                                                                                                                                                  atTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCaa  <  1:657459/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATAACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAA  >  NC_000913/3906679‑3906974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: