Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,500 G→T 5.3% intergenic (‑134/+49) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,5000GT 5.3% 328.9 / 7.8 115intergenic (‑134/+49)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (61/47);  new base T (4/2);  total (65/50)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

Only 100 of 115 total aligned reads displayed.

TCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGC  >  NC_000913/3908353‑3908648
                                                                                                                                                   |                                                                                                                                                    
tCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGcc                                                                                                                                                    <  2:132901/150‑1 (MQ=255)
tCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGcc                                                                                                                                                    >  1:1525190/1‑150 (MQ=255)
 cAACCATACTCATTGCAATCTTTTCCTAATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACTGACTCAAAAGGCGCTCGGTCGGTCCCCTCGcccc                                                                                                                                                  >  1:1466455/1‑150 (MQ=255)
       tACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                                                                                             >  1:70182/1‑150 (MQ=255)
        aCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                                                                                            <  2:2003185/150‑1 (MQ=255)
         cTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                                                                                           <  1:846706/150‑1 (MQ=255)
           cATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgag                                                                                                                                         <  2:1158053/150‑1 (MQ=255)
            aTTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGCTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                        <  1:1350063/150‑1 (MQ=255)
              tGCAATCTTTTCCTCATCGTGCCTGATGCACTGCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                                                                                      <  2:538045/150‑1 (MQ=39)
               gCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                     <  2:1542776/150‑1 (MQ=255)
               gCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                     <  1:1240430/150‑1 (MQ=255)
                cAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCGTCCGGGCAAGTGGTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCGTCTGGGGAGAgggg                                                                                                                                    >  2:385621/1‑149 (MQ=255)
                cAATCTTTTCCTCATCCTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                    <  2:818481/150‑1 (MQ=255)
                 aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                                                                                   >  1:369716/1‑150 (MQ=255)
                  aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                  >  1:1742147/1‑150 (MQ=255)
                  aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                  >  2:1190072/1‑150 (MQ=255)
                   tCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                 <  1:615726/150‑1 (MQ=255)
                    cTTTTCCTCATCGTGCCTGCTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCACAGGGGAGAGGGTTAgg                                                                                                                                >  1:220926/1‑150 (MQ=255)
                     ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                               <  2:1848145/150‑1 (MQ=255)
                     ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCAGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                               <  1:715055/150‑1 (MQ=255)
                         cgtCATCGTGCCTGCGGCACTTCGCTAATGAGGCTTACCGTAGGCCGGATCAGGGGTTCAGGCCGCATGCGGCAAAGTGGTACCCGTGTATAGTTTCTAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                           <  1:264920/148‑1 (MQ=37)
                          cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                          <  2:412879/150‑1 (MQ=255)
                          cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                          <  1:1846684/150‑1 (MQ=255)
                           tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                         <  1:134347/150‑1 (MQ=255)
                           tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                         <  1:860928/150‑1 (MQ=255)
                           tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                         <  1:1995262/150‑1 (MQ=255)
                            cATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                        >  2:8459/1‑150 (MQ=255)
                              tCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                      >  2:1466429/1‑150 (MQ=255)
                               cGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                     >  2:581537/1‑150 (MQ=255)
                                 tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGGGTTAACCGGGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                   <  1:1153785/150‑1 (MQ=255)
                                   ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAATCCg                                                                                                                 >  2:627030/1‑150 (MQ=255)
                                       ttgccgttCGCTCATCTGCCTTACCGTAGGCCGGAGCAGGCGTTCACGCCGCTTCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAATGGCGCTGGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTc                                                                                                             <  1:1845945/144‑1 (MQ=37)
                                        tGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCa                                                                                                            >  1:872560/1‑150 (MQ=255)
                                          cACTTCGCTAATCAGGCTTACCGTAGGCCGGCTCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGCGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                          >  1:1460317/1‑150 (MQ=255)
                                          cACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                          >  2:1561360/1‑150 (MQ=255)
                                              tCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCACATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCTCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGGGTCAGCAata                                                                                                      >  2:1934797/1‑150 (MQ=255)
                                                gCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACCCCGCATCCGGCAAAGTTTTAACCGTGTACAGAGTCAAAAAGGCGCGTGGTCGGTCCCGTCGCCCCTCTGGGGAGAGGGTTAGGGGGAGGGGAAAACCGTGTCAGCAATATc                                                                                                    <  2:522962/150‑1 (MQ=39)
                                                gCTAATCAGGCTGACCGTAGGCCGGATCAGGATTTCACGCCGCATACGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCTGTCGGACCCATCGCCCCTCACAGGAGTGGGTTAGGGTCAGGGGTAAAACGTGTCAGCAataac                                                                                                    >  1:1253869/1‑148 (MQ=255)
                                                   aaTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                                 >  1:1702506/1‑150 (MQ=255)
                                                      cAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgt                                                                                              >  2:1919134/1‑150 (MQ=255)
                                                       aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgtg                                                                                             <  1:8459/150‑1 (MQ=255)
                                                         gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGAGTCAGCAATATCAACCGTGtt                                                                                           >  2:812610/1‑150 (MQ=255)
                                                          cTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCTACCGTGgtt                                                                                          >  1:822158/1‑150 (MQ=255)
                                                           ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACAGTGTCAGCATTATCAAGCGGGGTTa                                                                                         >  2:685456/1‑150 (MQ=38)
                                                                   ggCCGGATCAGGGGTTCACGCCGCATCCGGCAAAGTGTTAACCTTGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGc                                                                                 <  2:357011/150‑1 (MQ=255)
                                                                   ggCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGc                                                                                 >  1:725685/1‑150 (MQ=255)
                                                                    gCCGGATCCGGCGGTCACGCCGCATCCGGCAAAGTGTTAACCGTGAACAGAGGCAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa                                                                                <  1:1169777/150‑1 (MQ=38)
                                                                    gCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACAGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAAGATCAACCGTGTGTATTCTTCGCa                                                                                >  1:290423/1‑150 (MQ=255)
                                                                     ccGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAACCCGTGTCAGCAATATCAACCGTGTTTATTCTTCGcca                                                                               >  2:1856650/1‑148 (MQ=255)
                                                                        gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAACCCGTGTCAGCAATATCACCCGTGTTTATTCTTCGCaaaaa                                                                            >  1:581532/1‑150 (MQ=255)
                                                                             ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcg                                                                       >  2:628037/1‑150 (MQ=255)
                                                                             ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCACCCGTGTTTATTCTTCGCAAAACCACcg                                                                       >  2:894484/1‑150 (MQ=255)
                                                                               cGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCACTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcg                                                                     >  1:1591120/1‑150 (MQ=255)
                                                                                      gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGa                                                              >  2:468911/1‑150 (MQ=255)
                                                                                           aTCCGGCAATGTGTTAACCGTTTACTGAGTCAAAAAGGCGCTGGGTCGGTCCCCTCCCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                         <  1:1462181/150‑1 (MQ=255)
                                                                                            tCCGGCAAAGTGTTAAGCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                        <  2:786629/150‑1 (MQ=255)
                                                                                            tCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                        <  2:1068931/150‑1 (MQ=255)
                                                                                                  aaaGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcagc                                                  <  2:179495/150‑1 (MQ=255)
                                                                                                      tgtTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGCCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                              <  1:575666/150‑1 (MQ=255)
                                                                                                      tgtAAACCGGGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                              <  2:1954712/150‑1 (MQ=255)
                                                                                                         tAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGc                                           <  1:222109/150‑1 (MQ=255)
                                                                                                            ccGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                                        >  2:943546/1‑150 (MQ=255)
                                                                                                            ccGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGCTTAGGGTCAGGGGAAAACCGAGTAAGCAATATCAACCGTGTTTATTCTACGCAAAAACAACGCGCGCCAGAATGTTAAGCAGCAGTACGcaca                                        >  1:867386/1‑150 (MQ=39)
                                                                                                             cGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                       >  1:1594157/1‑150 (MQ=255)
                                                                                                              gtttACATAGTCAAAAAGGCGCGCGGGCGGTCCCCTCGCCCCTGTGGGTAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                      <  1:1466429/147‑1 (MQ=39)
                                                                                                               gttACAGCGTCAAAAAGGCGCTCGGGCGGGCCCCGCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAggg                                     <  1:1571832/148‑1 (MQ=255)
                                                                                                                  aCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTaa                                  >  1:456741/1‑150 (MQ=255)
                                                                                                                   cAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAAt                                 >  1:1659342/1‑150 (MQ=255)
                                                                                                                    agagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCGCTTGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATg                                <  2:1525190/150‑1 (MQ=255)
                                                                                                                     gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGa                               >  2:194117/1‑150 (MQ=255)
                                                                                                                      agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                              <  2:447542/150‑1 (MQ=255)
                                                                                                                        tCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCa                            <  1:1190072/150‑1 (MQ=255)
                                                                                                                         cAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCTAAACCACCGCGCGCCAGAATGTTCCGCAGCAGTACGCCCAGGGTAATGATCaa                           >  1:733148/1‑150 (MQ=38)
                                                                                                                         cAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGGAATGCTCaa                           >  1:1110432/1‑150 (MQ=255)
                                                                                                                          aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCACAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACGTGGTAATGATCAAt                          >  2:1215472/1‑150 (MQ=255)
                                                                                                                               ggCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAcccc                     <  1:348225/150‑1 (MQ=255)
                                                                                                                                gcgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCg                    <  1:468911/150‑1 (MQ=255)
                                                                                                                                 cgcTCGGTCGGTCCCCTCGCCGCTCTGGGGAGAGGGTTAGGGGGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                   <  2:1850639/150‑1 (MQ=255)
                                                                                                                                 cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                   >  1:878850/1‑150 (MQ=255)
                                                                                                                                 cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                   <  1:581537/150‑1 (MQ=255)
                                                                                                                                    tcggtcggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCATAATGTTCAGCAGCAGTACGCACAGTGTAATGATCAATACCCCGGccc                >  2:1211826/1‑150 (MQ=255)
                                                                                                                                     cggtcggtCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCa               >  2:480949/1‑150 (MQ=255)
                                                                                                                                     cggtcggtCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGGGTTTAATCTTCGCAAAAACACAGCGCGCCAGAATGGTCAGCAGCAGTACGCACAGGGAAATGATCAATACCCCGGCCCa               >  2:322554/1‑150 (MQ=38)
                                                                                                                                        gcggccCCCTCGCCCCTCTGGGGCGCGGGTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc            <  1:1260711/149‑1 (MQ=255)
                                                                                                                                         cggtcCCCTCGCCCCTCTGGGTAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc           <  2:369716/150‑1 (MQ=255)
                                                                                                                                         cggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc           <  2:1725767/150‑1 (MQ=255)
                                                                                                                                         cggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc           >  1:943070/1‑150 (MQ=255)
                                                                                                                                          ggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa          >  1:1756886/1‑150 (MQ=255)
                                                                                                                                          ggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa          >  2:720460/1‑150 (MQ=255)
                                                                                                                                          ctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGGGTTTATTCTTCGCAAAATCAACGCGCGCCAGACTGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcga          >  1:1914016/5‑148 (MQ=255)
                                                                                                                                           gtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGccca         >  1:157531/1‑148 (MQ=255)
                                                                                                                                            tcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAAt        >  2:708073/1‑150 (MQ=255)
                                                                                                                                              cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGAAATAATGTTCAGCATCAGTACGCAAAGGGGAAGGATCAATACCCCGGGCGAGGCCAATtg      >  2:321939/1‑150 (MQ=255)
                                                                                                                                              cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCACCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTCCGCACAGGGTAATGCTCAATACCCCGGCCCAGGCCAATtg      >  1:1471556/1‑150 (MQ=255)
                                                                                                                                              cccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtg      >  1:418450/1‑150 (MQ=255)
                                                                                                                                               ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCCGCAATATCCCCCGTGTTTATTCTTCGCAAAAACAACGCTCGCCACCATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGTCCCAGGCCACTtgc     >  1:978511/1‑150 (MQ=38)
                                                                                                                                               ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgc     >  1:286645/1‑150 (MQ=255)
                                                                                                                                               ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgc     >  2:1659197/1‑150 (MQ=255)
                                                                                                                                                  cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctgc  >  1:661391/1‑150 (MQ=255)
                                                                                                                                                  cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGGCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctgc  <  2:70182/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGC  >  NC_000913/3908353‑3908648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: