Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,506 C→G 6.6% intergenic (‑140/+43) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,5060CG 6.6% 330.3 / 10.9 115intergenic (‑140/+43)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (62/45);  new base G (4/4);  total (66/49)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATT  >  NC_000913/3908360‑3908652
                                                                                                                                                  |                                                                                                                                                  
tACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                                                                                                 >  1:70182/1‑150 (MQ=255)
 aCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                                                                                                <  2:2003185/150‑1 (MQ=255)
  cTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                                                                                               <  1:846706/150‑1 (MQ=255)
    cATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgag                                                                                                                                             <  2:1158053/150‑1 (MQ=255)
     aTTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGCTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                            <  1:1350063/150‑1 (MQ=255)
       tGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                                                                                          <  1:613386/150‑1 (MQ=39)
       tGCAATCTTTTCCTCATCGTGCCTGATGCACTGCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                                                                                          <  2:538045/150‑1 (MQ=39)
        gCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                         <  1:1240430/150‑1 (MQ=255)
        gCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                         <  2:1542776/150‑1 (MQ=255)
         cAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCGTCCGGGCAAGTGGTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCGTCTGGGGAGAgggg                                                                                                                                        >  2:385621/1‑149 (MQ=255)
         cAATCTTTTCCTCATCCTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                        <  2:818481/150‑1 (MQ=255)
          aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                                                                                       >  1:369716/1‑150 (MQ=255)
           aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                      >  1:1742147/1‑150 (MQ=255)
           aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                      >  2:1190072/1‑150 (MQ=255)
              ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                   <  2:1848145/150‑1 (MQ=255)
              ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCAGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                   <  1:715055/150‑1 (MQ=255)
                  cgtCATCGTGCCTGCGGCACTTCGCTAATGAGGCTTACCGTAGGCCGGATCAGGGGTTCAGGCCGCATGCGGCAAAGTGGTACCCGTGTATAGTTTCTAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                               <  1:264920/148‑1 (MQ=37)
                   cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                              <  2:412879/150‑1 (MQ=255)
                   cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                              <  1:1688750/150‑1 (MQ=255)
                   cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                              <  1:1846684/150‑1 (MQ=255)
                    tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                             <  1:134347/150‑1 (MQ=255)
                    tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                             <  1:860928/150‑1 (MQ=255)
                    tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                             <  1:1995262/150‑1 (MQ=255)
                     cATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                            >  2:8459/1‑150 (MQ=255)
                        cGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                         >  2:581537/1‑150 (MQ=255)
                          tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGGGTTAACCGGGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                       <  1:1153785/150‑1 (MQ=255)
                            ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAATCCg                                                                                                                     >  2:627030/1‑150 (MQ=255)
                                ttgccgttCGCTCATCTGCCTTACCGTAGGCCGGAGCAGGCGTTCACGCCGCTTCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAATGGCGCTGGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTc                                                                                                                 <  1:1845945/144‑1 (MQ=37)
                                 tGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCa                                                                                                                >  1:872560/1‑150 (MQ=255)
                                   cACTTCGCTAATCAGGCTTACCGTAGGCCGGCTCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGCGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                              >  1:1460317/1‑150 (MQ=255)
                                   cACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                              >  2:1561360/1‑150 (MQ=255)
                                       tCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCACATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCTCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGGGTCAGCAata                                                                                                          >  2:1934797/1‑150 (MQ=255)
                                         gCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACCCCGCATCCGGCAAAGTTTTAACCGTGTACAGAGTCAAAAAGGCGCGTGGTCGGTCCCGTCGCCCCTCTGGGGAGAGGGTTAGGGGGAGGGGAAAACCGTGTCAGCAATATc                                                                                                        <  2:522962/150‑1 (MQ=39)
                                         gCTAATCAGGCTGACCGTAGGCCGGATCAGGATTTCACGCCGCATACGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCTGTCGGACCCATCGCCCCTCACAGGAGTGGGTTAGGGTCAGGGGTAAAACGTGTCAGCAataac                                                                                                        >  1:1253869/1‑148 (MQ=255)
                                          cTAATCAGGATTACCGTAGGCCGTATCAGGCGTTCAGGCCGCATCCGGAAAAGTGTTAACCGTGTACTGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCa                                                                                                       <  2:1788917/150‑1 (MQ=38)
                                               cAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgt                                                                                                  >  2:1919134/1‑150 (MQ=255)
                                                  gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGAGTCAGCAATATCAACCGTGtt                                                                                               >  2:812610/1‑150 (MQ=255)
                                                   cTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCTACCGTGgtt                                                                                              >  1:822158/1‑150 (MQ=255)
                                                    ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACAGTGTCAGCATTATCAAGCGGGGTTa                                                                                             >  2:685456/1‑150 (MQ=38)
                                                            ggCCGGATCAGGGGTTCACGCCGCATCCGGCAAAGTGTTAACCTTGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGc                                                                                     <  2:357011/150‑1 (MQ=255)
                                                            ggCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGc                                                                                     >  1:725685/1‑150 (MQ=255)
                                                             gCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGACAGCAATAACAACCGTGTTTATTCTTCGCa                                                                                    >  2:1273518/1‑150 (MQ=38)
                                                             gCCGGATCCGGCGGTCACGCCGCATCCGGCAAAGTGTTAACCGTGAACAGAGGCAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa                                                                                    <  1:1169777/150‑1 (MQ=38)
                                                             gCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACAGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAAGATCAACCGTGTGTATTCTTCGCa                                                                                    >  1:290423/1‑150 (MQ=255)
                                                              ccGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAACCCGTGTCAGCAATATCAACCGTGTTTATTCTTCGcca                                                                                   >  2:1856650/1‑148 (MQ=255)
                                                                 gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAACCCGTGTCAGCAATATCACCCGTGTTTATTCTTCGCaaaaa                                                                                >  1:581532/1‑150 (MQ=255)
                                                                      ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcg                                                                           >  2:628037/1‑150 (MQ=255)
                                                                      ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCACCCGTGTTTATTCTTCGCAAAACCACcg                                                                           >  2:894484/1‑150 (MQ=255)
                                                                        cGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCACTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcg                                                                         >  1:1591120/1‑150 (MQ=255)
                                                                               gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGa                                                                  >  2:468911/1‑150 (MQ=255)
                                                                                   cATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGt                                                              >  1:20029/1‑150 (MQ=255)
                                                                                    aTCCGGCAATGTGTTAACCGTTTACTGAGTCAAAAAGGCGCTGGGTCGGTCCCCTCCCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                             <  1:1462181/150‑1 (MQ=255)
                                                                                     tCCGGCAAAGTGTTAAGCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                            <  2:786629/150‑1 (MQ=255)
                                                                                     tCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                            <  2:1068931/150‑1 (MQ=255)
                                                                                               tgtTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGCCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                                  <  1:575666/150‑1 (MQ=255)
                                                                                               tgtAAACCGGGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                                  <  2:1954712/150‑1 (MQ=255)
                                                                                                  tAACGGTGTACTGAGTCAAAAAGGGGGGCGGTCGTTCGCCTCGCACCTCTGGGGCTTGGTTTAGGTTGAGGGGAAAACCGCGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAATCAACGCGCGCCAGAATGTTCAGCAGCAGTACGc                                               <  1:1273518/150‑1 (MQ=255)
                                                                                                  tAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGc                                               <  1:222109/150‑1 (MQ=255)
                                                                                                     ccGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                                            >  2:943546/1‑150 (MQ=255)
                                                                                                     ccGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGCTTAGGGTCAGGGGAAAACCGAGTAAGCAATATCAACCGTGTTTATTCTACGCAAAAACAACGCGCGCCAGAATGTTAAGCAGCAGTACGcaca                                            >  1:867386/1‑150 (MQ=39)
                                                                                                      cGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                           >  1:1594157/1‑150 (MQ=255)
                                                                                                       gtttACATAGTCAAAAAGGCGCGCGGGCGGTCCCCTCGCCCCTGTGGGTAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                          <  1:1466429/147‑1 (MQ=39)
                                                                                                        gttACAGCGTCAAAAAGGCGCTCGGGCGGGCCCCGCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAggg                                         <  1:1571832/148‑1 (MQ=255)
                                                                                                           aCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTTTTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTaa                                      >  1:379727/1‑150 (MQ=255)
                                                                                                           aCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTaa                                      >  1:456741/1‑150 (MQ=255)
                                                                                                            cAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAAt                                     >  1:1659342/1‑150 (MQ=255)
                                                                                                             agagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCGCTTGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATg                                    <  2:1525190/150‑1 (MQ=255)
                                                                                                              gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGa                                   >  2:194117/1‑150 (MQ=255)
                                                                                                               agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                  <  2:447542/150‑1 (MQ=255)
                                                                                                                gccAAAAAGGCGCGCGGTCGGCCCCCTCGCCCCTGTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATc                                 <  1:841226/148‑1 (MQ=255)
                                                                                                                 tCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCa                                <  1:1190072/150‑1 (MQ=255)
                                                                                                                  cAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCTAAACCACCGCGCGCCAGAATGTTCCGCAGCAGTACGCCCAGGGTAATGATCaa                               >  1:733148/1‑150 (MQ=38)
                                                                                                                  cAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGGAATGCTCaa                               >  1:1110432/1‑150 (MQ=255)
                                                                                                                   aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCACAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACGTGGTAATGATCAAt                              >  2:1215472/1‑150 (MQ=255)
                                                                                                                        ggCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAcccc                         <  1:348225/150‑1 (MQ=255)
                                                                                                                         gcgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCg                        <  1:468911/150‑1 (MQ=255)
                                                                                                                          cgcTCGGTCGGTCCCCTCGCCGCTCTGGGGAGAGGGTTAGGGGGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                       <  2:1850639/150‑1 (MQ=255)
                                                                                                                          cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                       <  1:581537/150‑1 (MQ=255)
                                                                                                                          cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                       >  1:878850/1‑150 (MQ=255)
                                                                                                                             tcggtcggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCATAATGTTCAGCAGCAGTACGCACAGTGTAATGATCAATACCCCGGccc                    >  2:1211826/1‑150 (MQ=255)
                                                                                                                              cggtcggtCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCa                   >  2:480949/1‑150 (MQ=255)
                                                                                                                              cggtcggtCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGGGTTTAATCTTCGCAAAAACACAGCGCGCCAGAATGGTCAGCAGCAGTACGCACAGGGAAATGATCAATACCCCGGCCCa                   >  2:322554/1‑150 (MQ=38)
                                                                                                                                  cggtcCCCTCGCCCCTCTGGGTAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc               <  2:369716/150‑1 (MQ=255)
                                                                                                                                  cggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc               >  1:943070/1‑150 (MQ=255)
                                                                                                                                  cggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc               <  2:1725767/150‑1 (MQ=255)
                                                                                                                                   ggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa              >  1:1756886/1‑150 (MQ=255)
                                                                                                                                   ggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa              >  2:720460/1‑150 (MQ=255)
                                                                                                                                   ctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGGGTTTATTCTTCGCAAAATCAACGCGCGCCAGACTGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcga              >  1:1914016/5‑148 (MQ=255)
                                                                                                                                    gtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGccca             >  1:157531/1‑148 (MQ=255)
                                                                                                                                     tcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAAt            >  2:708073/1‑150 (MQ=255)
                                                                                                                                       cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGAAATAATGTTCAGCATCAGTACGCAAAGGGGAAGGATCAATACCCCGGGCGAGGCCAATtg          >  2:321939/1‑150 (MQ=255)
                                                                                                                                       cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCACCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTCCGCACAGGGTAATGCTCAATACCCCGGCCCAGGCCAATtg          >  1:1471556/1‑150 (MQ=255)
                                                                                                                                        ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgc         >  1:286645/1‑150 (MQ=255)
                                                                                                                                        ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgc         >  2:1659197/1‑150 (MQ=255)
                                                                                                                                        ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgc         >  1:94328/1‑150 (MQ=255)
                                                                                                                                           cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctgc      >  1:661391/1‑150 (MQ=255)
                                                                                                                                           cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGGCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctgc      <  2:70182/150‑1 (MQ=255)
                                                                                                                                              cccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAt   >  2:1864190/1‑150 (MQ=255)
                                                                                                                                               ccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAtt  >  2:1692025/1‑150 (MQ=255)
                                                                                                                                               ccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAtt  >  1:1245010/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATT  >  NC_000913/3908360‑3908652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: