Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,809 G→A 5.5% P27L (CCG→CTG)  atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,8090GA 5.5% 441.2 / 8.3 146P27L (CCG→CTG) atpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base G (58/79);  new base A (2/6);  total (61/85)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01

Only 100 of 152 total aligned reads displayed.

GATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGA  >  NC_000913/3920661‑3920957
                                                                                                                                                    |                                                                                                                                                    
gatgatTACCTGGGCTGCCGCTTTCGCTTTTTGCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg                                                                                                                                                     <  2:88034/150‑1 (MQ=255)
 atgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                                                                    <  1:1603866/150‑1 (MQ=255)
 atgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                                                                    <  2:14733/150‑1 (MQ=255)
  tgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGcc                                                                                                                                                   <  2:184909/150‑1 (MQ=255)
  tgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGcc                                                                                                                                                   <  2:1291931/150‑1 (MQ=255)
  tgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTCCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGcc                                                                                                                                                   <  2:125595/150‑1 (MQ=255)
     ttACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATa                                                                                                                                                >  1:1158380/1‑150 (MQ=255)
      tACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTCGCCTTTGCAAGGTCAAGGTCCTTAAGTGATCGATCTGCGGAAGCAAGGACGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGCCGGCCATAc                                                                                                                                               >  2:1054682/1‑150 (MQ=255)
       aCCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTCGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACg                                                                                                                                              >  2:599831/1‑150 (MQ=255)
        ccTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                                                                                <  2:2005696/147‑2 (MQ=255)
        ccTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                                                                                >  1:2005696/1‑146 (MQ=255)
         ctcggCTTCCGCTTTCGCTATGTTCAGCTGGTCGCTCGCGCTGGACGTTGCAAGGTCACGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGtt                                                                                                                                            <  1:357905/147‑2 (MQ=255)
         cTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTTTGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTTATGGCGGCCATACGTa                                                                                                                                            >  1:1476515/1‑150 (MQ=255)
           gggCTTCCGCTTTCGCTTTTTTCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt                                                                                                                                          <  1:1942343/150‑1 (MQ=255)
            ggCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTTCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                                                                         >  1:629261/1‑150 (MQ=255)
             gCTTCCGCTTTCGCTTTTTTCAGCGGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCGTATGTGCGCGGTCTGCGGAAGCAAGGCCGTCAGCAAATTCTTTTTGACGTTTATCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                                                                                        <  1:1255835/150‑1 (MQ=255)
                 ccGCTTTCGCTTTTTTCAGCTGATCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                                                                    <  2:714861/150‑1 (MQ=255)
                  cgctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                                                                                   <  1:137963/150‑1 (MQ=255)
                   gctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                                                                  >  2:730826/1‑150 (MQ=255)
                   gctttcgctgtGTTCAGCTGGTCGGTCGCGCTGGCCGTTGCAAGGTCAAGGGCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                                                                  <  1:290785/150‑1 (MQ=255)
                    ctttcgctttTTTCAGGTGGTCGGTCGCGCTGGCCTTTGCAAGCTCAAGGTCGTTATGTGCTCGTTCTGCGGATGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcaga                                                                                                                                 <  1:245696/150‑1 (MQ=255)
                       tcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaaca                                                                                                                              <  1:1301421/150‑1 (MQ=255)
                          ctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagacaagaa                                                                                                                           >  1:1202042/1‑150 (MQ=255)
                          ctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacagaa                                                                                                                           >  2:1579364/1‑150 (MQ=255)
                          ctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacagaa                                                                                                                           >  1:1522330/1‑150 (MQ=255)
                          ctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGaagaacagac                                                                                                                           >  1:1051583/1‑149 (MQ=255)
                           ttttttCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                                                                                          <  2:1432217/150‑1 (MQ=255)
                           ttttttCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                                                                                          <  2:88699/150‑1 (MQ=255)
                                  aGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAgg                                                                                                                   <  2:1946966/150‑1 (MQ=255)
                                  aGCTGGTCGGGCGCGCTGTCCTTTGCAAGGGCAAGGTCCTTATGTGCTCGTTCTGCGTAAGCAAGGCCGTCAGATATTTGTTTTTTACGTTTTGAGATGGCTGCCATTTATGGCGGCCATACGTACTTCATGCAGAACTTAACGAACatt                                                                                                                   <  2:1201693/150‑3 (MQ=255)
                                    cTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGAc                                                                                                                 <  1:1121546/150‑1 (MQ=255)
                                     tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                                                                                >  1:1467535/1‑150 (MQ=255)
                                     tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                                                                                >  1:1459295/1‑150 (MQ=255)
                                         cggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcg                                                                                                            >  2:1423863/1‑150 (MQ=255)
                                         cggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGATCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGCCGTGTTTCGATGGCTGCCATTAATGGCGGCCATACTTACTTCATGCAGAACAGAACGAACAGGACACAcg                                                                                                            >  1:1283380/1‑150 (MQ=255)
                                          ggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGAAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcgc                                                                                                           <  2:1824474/150‑1 (MQ=255)
                                           gtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcgcg                                                                                                          >  1:770530/1‑150 (MQ=255)
                                             cgcgCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAt                                                                                                        >  1:394297/1‑150 (MQ=255)
                                             cgcgCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAt                                                                                                        >  2:1614381/1‑150 (MQ=255)
                                               ggcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                                                                      <  1:162814/149‑1 (MQ=255)
                                                 cTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCAATAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcc                                                                                                    >  1:1866772/1‑150 (MQ=255)
                                                   ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctg                                                                                                  <  1:201706/150‑1 (MQ=255)
                                                      cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctggcc                                                                                               >  2:1145859/1‑150 (MQ=255)
                                                       tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCg                                                                                              >  1:1994175/1‑150 (MQ=255)
                                                         tGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                                                                                            <  2:1845100/150‑1 (MQ=255)
                                                         tGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                                                                                            <  2:403127/150‑1 (MQ=255)
                                                         tGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                                                                                            >  1:1509674/1‑150 (MQ=255)
                                                          gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAgg                                                                                           <  1:945310/150‑1 (MQ=255)
                                                             aggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAtt                                                                                        >  1:802576/1‑150 (MQ=255)
                                                                tcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgtt                                                                                     >  2:1193455/1‑150 (MQ=255)
                                                                 caaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                                                                                    >  1:1258869/1‑150 (MQ=255)
                                                                  aaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTATTTTTGACGTTTTTCGATGGCTGCCAATAATGGCGGCCATACCTACTTCATGCAGAAAAGAACGAACAGGACAAACGCGAAGGCCTGGCCGAGGAATCTTGc                                                                                   >  1:202496/1‑150 (MQ=255)
                                                                      tcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                                                                               <  2:1598851/150‑1 (MQ=255)
                                                                      tcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGACGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                                                                               <  1:1093067/150‑1 (MQ=255)
                                                                        cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAg                                                                             <  2:588524/150‑1 (MQ=255)
                                                                         ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa                                                                            <  2:738289/150‑1 (MQ=255)
                                                                         ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGAACTTCATGCAGAACAGCACGAACAGGACACACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa                                                                            >  1:1420474/1‑150 (MQ=255)
                                                                          tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAt                                                                           <  2:939060/150‑1 (MQ=255)
                                                                                cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAg                                                                     <  2:248654/150‑1 (MQ=255)
                                                                                  cGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGca                                                                   <  1:24611/150‑1 (MQ=255)
                                                                                   gTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGcac                                                                  >  2:38823/1‑150 (MQ=255)
                                                                                       tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATg                                                              >  1:473581/1‑150 (MQ=255)
                                                                                       tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCAGAGGATTGTTGCGTTAAGATTCACAGCACAATg                                                              >  1:80118/1‑150 (MQ=255)
                                                                                        gCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGc                                                             >  2:205053/1‑150 (MQ=255)
                                                                                         cGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc                                                            >  2:1071274/1‑150 (MQ=255)
                                                                                         cGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc                                                            <  1:58672/150‑1 (MQ=255)
                                                                                              gCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAt                                                       >  2:35994/1‑150 (MQ=255)
                                                                                              gCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAt                                                       <  1:668190/150‑1 (MQ=255)
                                                                                                aaGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAttt                                                     <  2:1694802/150‑1 (MQ=255)
                                                                                                  ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGTCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAg                                                   <  1:1231417/150‑1 (MQ=255)
                                                                                                   gCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCTTACGTACTTCATGCAGATCAGATCGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGt                                                  <  1:1335846/150‑1 (MQ=255)
                                                                                                   gCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATAAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGt                                                  <  2:714542/150‑1 (MQ=255)
                                                                                                    ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGCCTAACGCGATGGGCTGGCCGCGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGtt                                                 >  2:353071/1‑150 (MQ=255)
                                                                                                      gTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTaa                                               <  1:447180/150‑1 (MQ=255)
                                                                                                       tCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCAGAATGCCTCTATTTAGTTAAc                                              <  1:1506843/150‑1 (MQ=255)
                                                                                                       tCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAAc                                              <  1:165685/150‑1 (MQ=255)
                                                                                                          gCAATTTCTTTTTGACGTTTTTCGATGGCTTCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACATGACAAACGCGATGGCCTGGCAGAGGATTATTGCGTTAAGATTCACAGCACAATGCATCTATTTAGTTAACGtt                                           >  2:1221007/1‑150 (MQ=255)
                                                                                                          gCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGtt                                           >  1:404787/1‑150 (MQ=255)
                                                                                                            aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAACCGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGATCt                                         >  2:1290458/1‑150 (MQ=255)
                                                                                                              tttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGa                                       <  2:1781153/150‑1 (MQ=255)
                                                                                                                  tttttGTCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACATACGCGAGGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATAtt                                   <  2:1283380/150‑1 (MQ=255)
                                                                                                                        aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCtt                             <  1:1633775/150‑1 (MQ=255)
                                                                                                                         cGTTTTTCGATGGCGGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCttt                            <  1:1758248/150‑1 (MQ=255)
                                                                                                                             tttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTtaaat                        <  2:1140003/150‑1 (MQ=255)
                                                                                                                              ttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTtaaata                       <  1:1831287/150‑1 (MQ=255)
                                                                                                                              acgtaTGGCCGCCATTAATGGAAGACATACGTACTTAATGAAGAACAGAACGAACAGGACAAAAGAGATGGCATGGCCGAGGATTGTTGCGGTAAGATTCACAGCACAATGACTCAAGTTAGTTAACGTTCTGATATTGCTCTTaaaata                       >  2:1400186/5‑150 (MQ=255)
                                                                                                                                cGATGGCTGCCATTAATGGCGTCCATACGTACTTCATGCAGAACACAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTtaaataaa                     <  2:1242108/150‑1 (MQ=255)
                                                                                                                                 taTGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATaaaa                    <  2:561619/149‑1 (MQ=255)
                                                                                                                                 gATGGCTGCTATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAATCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATaaaa                    <  1:286247/150‑1 (MQ=255)
                                                                                                                                  aTGGCTGCCATTAAAGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAg                   <  1:343692/150‑1 (MQ=255)
                                                                                                                                        gCCATTAATGGCGGCCATACGTACTTCCTGCAGAACAGCACGAACAGGACAATCGCGATGGCCTGGCCGAGGATTGGTGCGTTAAGATTCACAGCACAATGCCTCGATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGc             >  1:1227460/1‑150 (MQ=255)
                                                                                                                                        gCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGc             <  1:1756712/150‑1 (MQ=255)
                                                                                                                                          cATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCGTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCtt           <  2:442581/150‑1 (MQ=255)
                                                                                                                                          cATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTACATAAAAGCAACGCtt           >  1:1208209/1‑150 (MQ=255)
                                                                                                                                          cATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCtt           >  1:665310/1‑150 (MQ=255)
                                                                                                                                          cATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGAGATAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAATCAACGCtt           >  2:253408/1‑150 (MQ=255)
                                                                                                                                            ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTtac         <  2:1943239/150‑1 (MQ=255)
                                                                                                                                            ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTtac         <  2:1522330/150‑1 (MQ=255)
                                                                                                                                             tAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTtact        <  2:1039604/150‑1 (MQ=255)
                                                                                                                                                   cggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAGTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGa  >  1:1901386/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGA  >  NC_000913/3920661‑3920957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: