Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,923,718 G→C 5.3% intergenic (‑38/+26) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,7180GC 5.3% 472.0 / 12.7 147intergenic (‑38/+26)rsmG/mnmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base G (86/53);  new base C (5/3);  total (91/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

Only 100 of 148 total aligned reads displayed.

ACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAA  >  NC_000913/3923569‑3923865
                                                                                                                                                     |                                                                                                                                                   
aCGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     >  1:182073/1‑150 (MQ=255)
  gCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   >  2:636792/1‑150 (MQ=255)
    tttGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGTGGGTGc                                                                                                                                                 <  1:232390/150‑1 (MQ=255)
       gTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTa                                                                                                                                              >  2:1126003/1‑150 (MQ=255)
          ccAATTATGCAGCATATTCACGTAGGCAATAAGCAGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTTATGCGTTGCCTGGTAAGCACCCGCTTACCa                                                                                                                                           <  1:191556/150‑1 (MQ=255)
           cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCCGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                          <  1:169760/150‑1 (MQ=255)
           cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATAACTGATTCATTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGAGCTTACCAg                                                                                                                                          >  2:1304115/1‑150 (MQ=255)
            aTTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGACGGTGCTTACCAgg                                                                                                                                         >  1:207674/1‑150 (MQ=255)
             tttATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCATCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGc                                                                                                                                        >  2:253738/1‑150 (MQ=255)
                aTGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGTTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtt                                                                                                                                     >  1:662936/1‑150 (MQ=255)
                   cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGATTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAttttt                                                                                                                                  <  1:1664114/150‑1 (MQ=255)
                   cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                          <  2:290870/142‑1 (MQ=255)
                   cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTAACTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                          >  1:290870/1‑142 (MQ=255)
                      caTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAAt                                                                                                                               <  2:687536/150‑1 (MQ=255)
                      caTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTATTGATGCGTTGCCTGGTAATCGGGTGCTTACCAGGCATTTTTAAt                                                                                                                               >  1:294820/1‑150 (MQ=255)
                       atatTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGGCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATg                                                                                                                              <  1:135653/150‑1 (MQ=255)
                                tAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgcg                                                                                                                     >  1:1629529/1‑150 (MQ=255)
                                   gCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAATCGGGTGCTTACCAGGCTTTTTTAATGCGTTATGCGCTa                                                                                                                  >  1:900781/1‑150 (MQ=255)
                                     aaTAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTacg                                                                                                                <  2:587766/150‑1 (MQ=255)
                                       tAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATTCCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGACCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTacgac                                                                                                              <  1:363988/150‑1 (MQ=255)
                                         aGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGTAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgc                                                                                                            <  2:425992/150‑1 (MQ=255)
                                         aGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTAcgccgc                                                                                                            >  1:169590/1‑150 (MQ=255)
                                         aGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgc                                                                                                            >  2:317157/1‑150 (MQ=255)
                                          gCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTAAGCAGTAAGCAGAGTTAGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGATATGCGCTAAGACgca                                                                                                           >  2:1322987/1‑150 (MQ=255)
                                           cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcag                                                                                                          >  1:1356712/1‑150 (MQ=255)
                                           cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcag                                                                                                          >  1:1890982/1‑150 (MQ=255)
                                              gTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCAt                                                                                                       >  1:439347/1‑150 (MQ=255)
                                              gTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCGTACCAGGCATTTTTTATGCGTTATGCGCTACGACGCAGCAt                                                                                                       >  1:1268357/1‑150 (MQ=255)
                                                ttttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAc                                                                                                     <  2:1153735/150‑1 (MQ=255)
                                                   tCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCACTACGACGCAGCATACCCt                                                                                                  >  2:465081/1‑150 (MQ=255)
                                                    cTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGATGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTg                                                                                                 >  1:876713/1‑150 (MQ=255)
                                                      ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                               >  2:398003/1‑150 (MQ=255)
                                                         gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                            >  1:626468/1‑150 (MQ=255)
                                                         gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                            >  2:1547803/1‑150 (MQ=255)
                                                         gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                            <  1:1035763/150‑1 (MQ=255)
                                                            cGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATTCGCTACGACGCAGCATACCCTGTTTTTTCa                                                                                         >  1:145759/1‑150 (MQ=255)
                                                             ggTAAGCGAAATACCTGCGTCATTCAGAAGTAAGGAGAGTTTGTTGAGCACTGTGATAACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATATTTAATGCGTTAAGAGCTACTCCGCTCCATACCCTCTTTATTCAg                                                                                        >  1:959485/1‑150 (MQ=255)
                                                              gTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGGTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGc                                                                                       <  1:981519/150‑1 (MQ=255)
                                                                    gAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACAcc                                                                                 >  2:1470339/1‑150 (MQ=255)
                                                                    gAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACAcc                                                                                 <  1:738887/150‑1 (MQ=255)
                                                                       aTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagc                                                                              <  1:1971962/150‑1 (MQ=255)
                                                                             gCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATg                                                                        >  1:26803/1‑150 (MQ=255)
                                                                             gCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATg                                                                        >  2:582195/1‑150 (MQ=255)
                                                                              cGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATgg                                                                       <  2:516905/150‑1 (MQ=255)
                                                                                    tCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTAACAGGCATTTTTAATGCGTTATTCGCTACGACGCAGCATACCCTGTTTTTTAAGCCACACCAGCAGAATGGAGAttg                                                                 >  1:1932616/1‑148 (MQ=255)
                                                                                     cagcagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATgac                                                                <  1:235470/150‑3 (MQ=255)
                                                                                      agcagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGcc                                                               >  2:847493/1‑150 (MQ=255)
                                                                                       gcagTAAGGAGAGTTTGGTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCAGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCg                                                              <  2:214223/150‑1 (MQ=255)
                                                                                        cagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGATATTGCCGc                                                             >  1:1886409/1‑150 (MQ=255)
                                                                                         agTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCa                                                            <  2:1045213/150‑1 (MQ=255)
                                                                                         agTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTGCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTTAGACGCAGCATAACCTGTTTTTTCCGCCCCCCCAGCAGAATGGAGATGGCCGcc                                                            >  2:1496098/1‑149 (MQ=255)
                                                                                          gTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAg                                                           <  1:999720/150‑1 (MQ=255)
                                                                                            aaGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGAATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATTGCCGCAGGc                                                         >  2:793547/1‑150 (MQ=255)
                                                                                             aGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCg                                                        >  2:398864/1‑150 (MQ=255)
                                                                                             aGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGAAGAATGGAGATGGCCGCAGGCg                                                        >  1:10224/1‑150 (MQ=255)
                                                                                              ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                       >  2:2009535/1‑150 (MQ=255)
                                                                                               gagaGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTg                                                      <  2:969337/150‑1 (MQ=255)
                                                                                                       gttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATTCGCTACGACGCAGCATACCCAGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGa                                              >  1:851729/1‑150 (MQ=255)
                                                                                                       gttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGa                                              >  2:1598064/1‑150 (MQ=255)
                                                                                                        ttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaa                                             <  2:1657091/150‑1 (MQ=255)
                                                                                                         tgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTAGCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaaa                                            <  2:176348/150‑1 (MQ=255)
                                                                                                         tgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaaa                                            >  2:766053/1‑150 (MQ=255)
                                                                                                             cACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcg                                        >  2:1683365/1‑150 (MQ=255)
                                                                                                             cACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcg                                        >  2:1288329/1‑150 (MQ=255)
                                                                                                             cACGGTGATTACCTGTTCTTGATGCGTGGCCTGGTAAGCGGGTGCTTACCAGGCATTTATAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGAGGGCCGCTGGCGTGACGCCAGAAATAcg                                        <  1:321471/150‑1 (MQ=255)
                                                                                                               cGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcgcg                                      <  1:1362874/150‑1 (MQ=255)
                                                                                                               cGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcgcg                                      <  1:1198451/150‑1 (MQ=255)
                                                                                                                  tGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAg                                   >  1:967501/1‑150 (MQ=255)
                                                                                                                      tACCTGTTCTTGATGCGTTGCCAGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAAACGCGAAGCGTg                               >  2:943120/1‑150 (MQ=255)
                                                                                                                        ccTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCGTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAACCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGc                             >  1:1002021/1‑150 (MQ=255)
                                                                                                                         cTGTTCTTGAGGCGTTTCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                            <  2:835928/150‑1 (MQ=255)
                                                                                                                          tGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCg                           <  1:253738/150‑1 (MQ=255)
                                                                                                                          tGTACTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCGAGAAATACGCGAAGCGTGGCCg                           >  1:89446/1‑150 (MQ=255)
                                                                                                                           gTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGa                          >  1:1110947/1‑150 (MQ=255)
                                                                                                                           gTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGa                          >  2:1837916/1‑150 (MQ=255)
                                                                                                                               ttGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATaga                      <  1:1223753/150‑1 (MQ=255)
                                                                                                                                tGATGCGTTGCCTGGTGAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTTCGACGCAGCATACCATGTTTTTTCAGCCACACAAGAAGTATGGTGATGTCCGCAGGCGTGACGCCAGAAATACGCGTAGCTTGGCCGATagag                     >  1:1283585/1‑150 (MQ=255)
                                                                                                                                 gATGCGTTGCCTGGTAAGCGGGTGCATACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTAAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                    <  2:279697/150‑1 (MQ=255)
                                                                                                                                  aTGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGc                   <  1:1357886/150‑1 (MQ=255)
                                                                                                                                   tGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACGCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCt                  >  1:304444/1‑150 (MQ=255)
                                                                                                                                    gCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTg                 >  1:2009264/1‑150 (MQ=255)
                                                                                                                                     cGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTgg                >  2:35914/1‑150 (MQ=255)
                                                                                                                                       ttGGCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGtt              <  2:182073/150‑1 (MQ=255)
                                                                                                                                         gCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGGAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGAGGATTGAGGCTGGtttt            >  2:389642/1‑149 (MQ=255)
                                                                                                                                         gCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtg            >  2:1869432/1‑150 (MQ=255)
                                                                                                                                          ccTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt           >  2:226624/1‑150 (MQ=255)
                                                                                                                                          ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt           >  1:373136/1‑150 (MQ=255)
                                                                                                                                          ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt           >  2:1417079/1‑150 (MQ=255)
                                                                                                                                           cTGGTAAGCGGGTGCTTACCAGGCATTTTTAATTCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgtg          <  1:1386667/150‑1 (MQ=255)
                                                                                                                                           cTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGCGGCTGGTTtgtg          >  2:1162581/1‑150 (MQ=255)
                                                                                                                                           cTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgtg          >  1:1767333/1‑150 (MQ=255)
                                                                                                                                           cTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgtg          >  2:496688/1‑150 (MQ=255)
                                                                                                                                            tGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGCGGCTGGTTTGTGa         >  1:746182/1‑150 (MQ=255)
                                                                                                                                            tGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGa         >  1:1090400/1‑150 (MQ=255)
                                                                                                                                            tGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGa         >  2:1422637/1‑150 (MQ=255)
                                                                                                                                            tGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGa         >  2:1283047/1‑150 (MQ=255)
                                                                                                                                              gTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATc       <  2:26803/150‑1 (MQ=255)
                                                                                                                                               tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCg      <  2:1125769/150‑1 (MQ=255)
                                                                                                                                                 aGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGtt    >  1:708763/1‑150 (MQ=255)
                                                                                                                                                   cGGGTGCTTACCAGGCATTTTAAATGCGTTATGCGCTACGACGCAGCATTCCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGATATACGCGAAGCTTGTACAATAGAGGCTGGTTTGTGATCTTTaa  >  2:332234/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAA  >  NC_000913/3923569‑3923865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: