Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,515 T→A 7.0% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5150TA 7.0% 399.7 / 11.1 152intergenic (+70/+17)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (64/73);  new base A (5/7);  total (71/81)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

CAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAC  >  NC_000913/3959367‑3959662
                                                                                                                                                    |                                                                                                                                                   
 aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                                                                                   <  1:1267876/150‑1 (MQ=255)
  gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                                                  <  2:851203/150‑1 (MQ=255)
    cATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGCGAGGGAGAAAACACTCAAGGCCTTCTCCTGgaga                                                                                                                                                >  2:1936057/1‑150 (MQ=255)
     ttGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGAGAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGaa                                                                                                                                               <  2:1043294/149‑1 (MQ=255)
      tGCGATTGAGCAGGTAGGTAAGAAACTGCGCTGCTATATGACAGATATGAAACGTTTTGCTGTTTCGGGTTAAGTGCGCGCTGATGCACTCACCCCGACCCTCTCCCACAGGGAGAGGGATAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                                                                              <  1:1361925/150‑1 (MQ=38)
      tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                                                                              <  2:35809/150‑1 (MQ=255)
        cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                                                                            >  2:1143722/1‑150 (MQ=255)
        cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGc                                                                                                                                            <  1:856648/150‑1 (MQ=255)
          tttGAGCAGGTAGGTAAGAATCAGCGCGGCTATATGACAGATATGAACCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCt                                                                                                                                          <  1:422444/149‑1 (MQ=255)
          aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGACACGTATTACTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                                          <  1:71079/150‑1 (MQ=255)
          aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                                          <  1:1970359/150‑1 (MQ=255)
          aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                                          <  1:551289/150‑1 (MQ=255)
          aTTGAGCAGGGAGGTAAGACACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                                          <  1:164083/150‑1 (MQ=255)
           ttGAGCAGGTCGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGGATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCtt                                                                                                                                         <  2:160624/150‑1 (MQ=255)
           ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCtt                                                                                                                                         <  1:594288/150‑1 (MQ=255)
             gaggagGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGGGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAATACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                                                                                       <  2:786895/150‑1 (MQ=255)
             gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                                                                                       <  1:1353594/150‑1 (MQ=255)
               gCAGGTAGGTAAGAAACTGCGCGGCTATATGCCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgagt                                                                                                                                     <  2:1379029/150‑4 (MQ=255)
                      ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                                                                              >  1:1383399/1‑150 (MQ=255)
                       gtaAGAAACTTCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTACGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                                                                             <  2:1608928/150‑1 (MQ=255)
                       gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATAGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCGACCCTCTCCCACAGGGTGAGGGAGCAAACACTCAAGGCCTTCTCCTGGAGACGGCGGTGCTATTAGTAGc                                                                                                                             >  2:212393/1‑150 (MQ=255)
                       gcaaGAAACTGCGCGGCTATATGACCGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCCCCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                                                                             <  1:105066/148‑1 (MQ=255)
                         aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCACTCACCCCGACCCTCTCCCACAGGGAGAGGCAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                                                           >  2:1350482/1‑150 (MQ=255)
                          aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt                                                                                                                          <  2:1966511/150‑1 (MQ=255)
                            aaccTGCGCGGCAATCTGACAGATATGAAACGTATTGCTGTTGCGGGTTCAGTGCGCTCAGATGCCCTCACCCCGACCCTCTCCCACATGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                                                                        <  2:385851/147‑1 (MQ=255)
                            aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                                                                        <  2:1706965/150‑1 (MQ=255)
                            aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                                                                        <  1:1806564/150‑1 (MQ=255)
                              aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACACCGACCCTCACCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCACCAGGAGAAGGCCATGCTATTAGTTCCCGTACAg                                                                                                                      >  2:1278433/1‑150 (MQ=38)
                               cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                                                                                     <  1:642933/150‑1 (MQ=255)
                               cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                                                                                     >  1:1889417/1‑150 (MQ=255)
                               cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGCGAGAAAACACTCAAGTACTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                                                                                     >  2:1060739/1‑150 (MQ=255)
                                        tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatg                                                                                                            <  1:1947934/150‑1 (MQ=255)
                                        tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatg                                                                                                            <  1:1143722/150‑1 (MQ=255)
                                          tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCGACCCTCGCCCCCCGGGAGAGGGAGCAAACCCACAAGGCCATATCCTGGAGAAGGCCGTGCTATTAGTTGCGGTTCCGCCCCGTTatgat                                                                                                          >  1:1779284/1‑150 (MQ=255)
                                          tatGACAGATATGAAACGTATTGATGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAAAGCACCTTAatgat                                                                                                          >  1:54846/1‑150 (MQ=255)
                                           atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                                         <  2:1323719/150‑1 (MQ=255)
                                            tGACAGATATGAAACGTATTGGTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                                        <  2:1389121/150‑1 (MQ=255)
                                             gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                                                                                       >  2:72744/1‑150 (MQ=255)
                                               cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                                                                                                     <  2:1545599/150‑1 (MQ=255)
                                                            tATTGCTGTTGCGGTTTAAGTGCGCGCTGATGCCCTCACGCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCGTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                                        <  2:1586456/150‑1 (MQ=255)
                                                              ttgattttGCGGGTTAAGTGAGTTTTGATTCACTCACTCCTACTCTCTAAATCTGGGTGAGGGAGAAAACACTCAAGGCCTTCTCCTGGATAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                                                                      <  2:950990/144‑1 (MQ=255)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGGTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg                                                                                  >  2:1347684/1‑150 (MQ=255)
                                                                   tttGCGGGTTAAGGGGGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc                                                                                 <  2:1252747/149‑1 (MQ=255)
                                                                     tGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                                                                               <  1:1696268/150‑1 (MQ=255)
                                                                       cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                             <  2:1719700/150‑1 (MQ=255)
                                                                          gTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCACCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCgg                                                                          <  2:1943604/150‑1 (MQ=255)
                                                                            tAAGTGCGCGCTGATGCCCGCACCCCGACCCTCTCCCACCGGGAGAGGGATAAAACACTCAACGCCTTCTGCTGGAGAAGGCCTTGCTATTAGTTGCGGGACAGCACCATCATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                                                        <  2:298219/150‑1 (MQ=255)
                                                                              aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                      <  1:128429/150‑1 (MQ=255)
                                                                                tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCt                                                                    >  2:2013942/1‑150 (MQ=255)
                                                                                tGCGCGCTAATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGcg                                                                    >  1:450272/1‑149 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                 <  2:1953802/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                 <  1:506591/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTAACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                 >  1:1280227/1‑150 (MQ=255)
                                                                                    cgcTGATGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCAGGCCGTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTCATATCCGAACTGGGGGTGCAGCGGGCCGGTCGGCTCCAg                                                                <  1:122042/150‑1 (MQ=38)
                                                                                    cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAg                                                                <  2:6212/150‑1 (MQ=255)
                                                                                      cTGATGCCCTCACCCCGACCCTCTCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                                                             >  2:915729/1‑150 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTTATGATGTGATATCCGTACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                            >  2:1301158/1‑150 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATTTGATATCCGCACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                            >  2:509590/1‑150 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                            >  1:665734/1‑150 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                            <  2:1884507/150‑1 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                            >  1:1961328/1‑150 (MQ=255)
                                                                                            cccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGAc                                                        >  1:1732407/1‑150 (MQ=255)
                                                                                                cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                                    >  1:1472125/1‑150 (MQ=255)
                                                                                                 aCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAagag                                                   <  2:1586951/150‑1 (MQ=255)
                                                                                                  ccccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCGCCGGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAgaga                                                  >  1:851808/1‑150 (MQ=255)
                                                                                                    ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCCCCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                                                >  1:568636/1‑150 (MQ=255)
                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                               <  2:1510810/150‑1 (MQ=255)
                                                                                                      gACCCTCTCCCACAGGGAGAGTGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTTGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc                                              <  1:361783/150‑1 (MQ=255)
                                                                                                        cccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCGCCTTAAGGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGCCAAGAGTAAACCa                                            >  2:515740/1‑150 (MQ=255)
                                                                                                        cccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCTGTACAGAACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCCGGCAGGTCGGCTACAGTACCGGACAAGAGAAAACCa                                            >  1:1250146/1‑150 (MQ=255)
                                                                                                        cccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                            >  2:1864350/1‑150 (MQ=255)
                                                                                                         ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc                                           <  1:712050/150‑1 (MQ=255)
                                                                                                            atcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACttt                                        <  2:571016/149‑1 (MQ=255)
                                                                                                             tcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAATACCGGACAAGAGAAAACCACTTTa                                       <  1:26478/150‑1 (MQ=255)
                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGCTCGGCTCCAGTACCGGACAAGAGAAAACCACGTTATCGa                                   >  1:396831/1‑150 (MQ=255)
                                                                                                                  caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa                                  >  1:1534886/1‑150 (MQ=255)
                                                                                                                    gggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTAGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACg                                >  2:284747/1‑150 (MQ=255)
                                                                                                                     ggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGCACGc                               >  2:341186/1‑150 (MQ=255)
                                                                                                                     ggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc                               >  2:882499/1‑150 (MQ=255)
                                                                                                                        gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAACACCACTTTATCGAACGCCgg                            >  1:402875/1‑150 (MQ=255)
                                                                                                                        gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                            <  2:317739/150‑1 (MQ=255)
                                                                                                                          gggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa                          >  1:1739878/1‑150 (MQ=255)
                                                                                                                             agaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCa                       >  2:895961/1‑150 (MQ=255)
                                                                                                                              gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                      <  1:246367/150‑1 (MQ=255)
                                                                                                                              gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGCACAAGAGAAAACCACTATATCGAACGCCGGAACCAt                      >  1:1105063/1‑150 (MQ=255)
                                                                                                                                 aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTAGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGAAGCGGGCCGTTCGGCTCCATTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg                   >  2:139202/1‑150 (MQ=255)
                                                                                                                                   cacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTTGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAc                 >  2:1802719/1‑150 (MQ=255)
                                                                                                                                   cacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGACGGAACCATCTGAc                 >  1:1402215/1‑150 (MQ=255)
                                                                                                                                   cacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATACGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTgcc                 >  2:818753/1‑148 (MQ=255)
                                                                                                                                     cTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTTTTAGTTGCGGTACCGCACCTTATTGATGTGATATCCGAGCTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAccc               >  1:1496920/1‑150 (MQ=255)
                                                                                                                                     cTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGGAGGCTCGAGTACCGGACAAGAGAAAACCACTTTAGCGAAAGCCGGAACCATATGAccc               >  1:1638250/1‑150 (MQ=255)
                                                                                                                                      gcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt              >  2:920840/2‑150 (MQ=255)
                                                                                                                                      gcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGATGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTATATCGAACGCCGGAACCATCTCACCCt              >  1:359260/2‑150 (MQ=255)
                                                                                                                                        aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg            >  2:1797644/1‑150 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACGTTAATGATGTGATATCCGAACTGGGTGAGCAGCGGGCCGGTCTGCTCCAGTAACGGACAAGAGAAAACCACTTTATCGAACGCCGGAACAATCTGAACCTGGCGGaa       >  2:111178/1‑150 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGCTCCATCTGACACTGGCGGaa       >  1:1535546/1‑150 (MQ=255)
                                                                                                                                               tctgCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGTAACCATCTGACCCTGGCGGAAtt     <  2:1868489/150‑1 (MQ=255)
                                                                                                                                                ctcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTc    >  1:470049/1‑150 (MQ=255)
                                                                                                                                                 tcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGTTGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCa   <  2:1383399/150‑1 (MQ=255)
                                                                                                                                                  ccTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGAACCTTAATGATGTGATATCCGATCTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAc  <  2:1234441/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAC  >  NC_000913/3959367‑3959662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: