Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,925 A→T 8.1% intergenic (‑112/+87) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,9250AT 8.1% 370.7 / 12.1 128intergenic (‑112/+87)ppiC/yifNpeptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
Reads supporting (aligned to +/- strand):  ref base A (62/55);  new base T (5/6);  total (67/61)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCA  >  NC_000913/3959779‑3960073
                                                                                                                                                  |                                                                                                                                                    
aCAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATc                                                                                                                                                   >  2:634720/1‑150 (MQ=255)
 cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATACTTGCCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                  <  1:893061/150‑1 (MQ=255)
 cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                  >  2:1570038/1‑150 (MQ=255)
 cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                  >  2:483190/1‑150 (MQ=255)
 cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                  >  1:1536411/1‑150 (MQ=255)
   aGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGCTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCgg                                                                                                                                                >  2:883127/1‑150 (MQ=255)
   aGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCgg                                                                                                                                                <  2:231578/150‑1 (MQ=255)
    ggATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCggg                                                                                                                                               <  1:1852378/150‑1 (MQ=255)
     gATCTGCAGTGCGGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGc                                                                                                                                              <  2:846896/150‑1 (MQ=255)
        atgccttgctgatgcTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATAGGCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttt                                                                                                                                           <  1:785052/144‑2 (MQ=255)
        atGCAGTGCTGCTGCTGTTTTTGCCATGATCGAGCCTTGACTGACTAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttt                                                                                                                                           <  1:39991/150‑2 (MQ=255)
         tGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGCTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCg                                                                                                                                          >  1:1090866/1‑150 (MQ=255)
          gCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATACTTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttg                                                                                                                                         <  1:915729/150‑4 (MQ=255)
           cAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCa                                                                                                                                        <  2:461603/150‑1 (MQ=255)
            aGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAg                                                                                                                                       <  2:1765281/150‑1 (MQ=255)
            aGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAg                                                                                                                                       >  2:439585/1‑150 (MQ=255)
               gctgctgctGTTTTTGCCATGATCGTGCCTTGAGTGACTAATTGTCAGGCGGCGATGTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTc                                                                                                                                    <  1:738205/150‑1 (MQ=255)
               gctgctgctGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTc                                                                                                                                    >  1:426663/1‑150 (MQ=255)
                 tgctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTccc                                                                                                                                  >  1:1460308/1‑150 (MQ=255)
                 tgcggctgTTTTTGCCATGATCGTGCCTTGAGTGAATACTTCTCCGGCGGCGAGTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGcccc                                                                                                                                  <  1:392487/150‑1 (MQ=255)
                 ggctgctgctTTTGCCATGAGCGTGCCTTGAGGGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGGccc                                                                                                                                  <  1:212393/149‑1 (MQ=255)
                  gctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATAGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCt                                                                                                                                 <  1:344486/150‑1 (MQ=255)
                   ctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTc                                                                                                                                <  2:260527/150‑1 (MQ=255)
                       tgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgg                                                                                                                            <  2:1669589/150‑1 (MQ=255)
                        gTTTTTGCCATGATCGTCCCTTGAGTTAATATTTGTCAGGCGGCTATTTTAGCATGATCCGCAGATCCTAACGTAATTGTCTGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAggg                                                                                                                           <  1:496159/150‑1 (MQ=255)
                         tttttGCCATGATCGTGCCTTGACGGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACCTAAATGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgggg                                                                                                                          <  2:1496920/150‑1 (MQ=255)
                           tttGCCATGCTCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGgag                                                                                                                        <  2:1908641/150‑1 (MQ=255)
                              gCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaa                                                                                                                     >  2:752749/1‑150 (MQ=255)
                              gCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaa                                                                                                                     >  1:833048/1‑150 (MQ=255)
                               ccATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                    >  1:1351758/1‑150 (MQ=255)
                                cATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAGAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAc                                                                                                                   <  2:748069/150‑1 (MQ=255)
                                cATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAc                                                                                                                   <  1:801495/150‑1 (MQ=255)
                                 aTGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACt                                                                                                                  <  1:1572655/150‑1 (MQ=255)
                                   gATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTa                                                                                                                <  2:416833/150‑1 (MQ=255)
                                        tGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                                                           <  1:1144626/150‑1 (MQ=255)
                                         gCCTTGAGTGACTTATTGTCAGGCGGCGATTTTAGCATGATCCTCAGATGCTAACGTAATTCTCAGTCAGGGAGTGGCTGGTACATATATGGCAAACGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCct                                                                                                          <  2:1250146/150‑1 (MQ=255)
                                         gCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCct                                                                                                          >  2:1185834/1‑150 (MQ=255)
                                               aGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                                                                    >  1:729653/1‑150 (MQ=255)
                                                  gAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                                                                 >  2:1007092/1‑150 (MQ=255)
                                                  gAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAAGTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCCAGGGGAGAAACCTTAGGGCCTCTATGGGCt                                                                                                 >  2:1275736/1‑150 (MQ=255)
                                                   aataatTGTCAGGCGGCGTTTTTCGCATGATCCGCAGATGCGAACGTAATGGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAACGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTa                                                                                                <  1:1236341/150‑1 (MQ=255)
                                                        ttGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCTGTCAGGGAGTGGCCGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                                                                           <  2:151845/150‑1 (MQ=255)
                                                          gTCAGGCGGCGATTTTAGCATGATCCGCAGATGCGAACGTAATTGGCAGGCAGGGAGTGGCTGGTACATATATTGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAATGGGAGTAAAGTTAGGGCCTCTATTGGCATCAAAGta                                                                                         >  2:1949773/1‑149 (MQ=255)
                                                           tCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                                                                        <  1:780188/150‑1 (MQ=255)
                                                            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                                                                                       >  2:256662/1‑150 (MQ=255)
                                                            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAAATGTCACTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                                                                                       <  2:814205/150‑1 (MQ=255)
                                                            cAGGCGGCGATTTTAGCATGATCCGCAGATCCTAACGTAATAGACAGTCACGGAGTGGCAGGTACATATATGGCAAAACCCCGGAAAATCCGGGCTTCCCAGGTCCCTCAAGCGGTGAAAACTTAGGGCCACTAAGGCCTACAAAGTCAt                                                                                       >  1:186738/1‑150 (MQ=255)
                                                                   ggATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                                                                <  2:779244/149‑1 (MQ=255)
                                                                    gATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                                                               <  2:1584986/150‑1 (MQ=255)
                                                                         tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                                                                          <  1:1007092/150‑1 (MQ=255)
                                                                         tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                                                                          <  1:1032787/150‑1 (MQ=255)
                                                                             aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGTGTGGCTGGTACATATATGGCAAAAGCCCGGATAATCCGGGCTTCGCAGGTCCCTCTAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                                                                      <  1:461257/150‑1 (MQ=255)
                                                                             aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                                                                      >  1:489841/1‑150 (MQ=255)
                                                                             aTGATCCGCAGAGGCTAACGTAATTGGCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                                                                      <  1:1681497/150‑1 (MQ=255)
                                                                              tGATCCGCAGATGCTAACGTAATTGACAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAACGGGAGAAAAGTTAGGGCCTCTATTGGCTACAAAGTCATTATCTGGGATGGttattt                                                                     >  2:964492/1‑150 (MQ=255)
                                                                               tctCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttt                                                                    <  1:1760372/148‑1 (MQ=255)
                                                                                aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                                                                   >  1:358594/1‑150 (MQ=255)
                                                                                   cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                                                                <  1:1945297/150‑1 (MQ=255)
                                                                                    gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATCGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt                                                               <  1:917993/150‑1 (MQ=255)
                                                                                        aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAt                                                           >  1:1555965/1‑150 (MQ=255)
                                                                                        aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAt                                                           >  1:1547903/1‑150 (MQ=255)
                                                                                           cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAtttt                                                        >  1:1846548/1‑149 (MQ=255)
                                                                                               cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg                                                    >  2:1999783/1‑150 (MQ=255)
                                                                                                   aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat                                                <  2:1944380/150‑1 (MQ=255)
                                                                                                    ttttcagtcagGGCGTGGCAGGTACATATATGGCTAAAGACAGTAAAATCAGTGCTTCGCAGGTGCCTAAAGGGGATAAAACTTAGGGCCTCTATGTGCTAAAAAGTCATTATCGGGGATGGTTAATTTATTGTCAATTTATGAGCGata                                               <  1:95755/147‑1 (MQ=255)
                                                                                                    ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGGTATTTTATTGACAATTTAAGAGAGata                                               >  2:282188/1‑150 (MQ=255)
                                                                                                    ttGTCAGTCAGGGAGGGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata                                               <  1:356744/150‑1 (MQ=255)
                                                                                                     tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat                                              >  1:1825605/1‑150 (MQ=255)
                                                                                                         agtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCa                                          >  2:1564191/1‑150 (MQ=255)
                                                                                                          gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCat                                         >  2:709193/1‑150 (MQ=255)
                                                                                                          gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCat                                         >  2:1796514/1‑150 (MQ=255)
                                                                                                            cagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTCTCAATTTATGAGCGATATGGCattg                                       >  1:1388790/1‑150 (MQ=255)
                                                                                                               ggAGTGGCTGGTACATATATGGCAAAAGGCCTGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTACGGCCTCTATGGGCTACAAAGTGATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCattgatt                                    <  1:270019/150‑1 (MQ=255)
                                                                                                               ggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCattgatt                                    >  1:1506822/1‑150 (MQ=255)
                                                                                                                gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCattgattg                                   <  2:90726/150‑1 (MQ=255)
                                                                                                                    ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACtt                               >  1:1499371/1‑150 (MQ=255)
                                                                                                                    ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATATTATTGTAAATTTATGAGCGATATGGCATTGATTGACtt                               >  1:96501/1‑150 (MQ=255)
                                                                                                                     gCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACttt                              >  1:439077/1‑149 (MQ=255)
                                                                                                                      cTGGTTCATATATTTCTAAAGCCCTGATTATCCGGGCTTCGCAGGTCCCTCAAGGGGAGATATCTTATGGCCTCTATGGGCTACAAAGTCATTTTCGGGGATGGTTATTTTATTGTCAATTTATGAGCTATATGGCATTGATTGACTtga                             <  1:218673/150‑1 (MQ=255)
                                                                                                                        ggTACATATATGTCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTTTTTTATTGTCAATTTATGAGAGATATGGCATTGATTGACTtgatg                           >  2:378952/1‑150 (MQ=255)
                                                                                                                        ggTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtgatg                           <  2:729653/150‑1 (MQ=255)
                                                                                                                              tatatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGcc                     >  1:674651/1‑150 (MQ=255)
                                                                                                                                 atGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCctc                  >  1:144072/1‑150 (MQ=255)
                                                                                                                                 atGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCctc                  >  1:1044883/1‑150 (MQ=255)
                                                                                                                                  tGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCctct                 >  1:1023383/1‑150 (MQ=255)
                                                                                                                                   ctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTg                >  1:804020/6‑150 (MQ=255)
                                                                                                                                    tgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGt               >  1:1740151/5‑150 (MQ=255)
                                                                                                                                    gCAAAAGCCCGGAAAATCCGGGCTTCGCCGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGACATGGCATTGATTGACTTGATGACCGCCCTCTGt               <  2:764393/150‑1 (MQ=255)
                                                                                                                                    gCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGTGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGt               >  2:954542/1‑150 (MQ=255)
                                                                                                                                    gCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGt               <  1:534456/150‑1 (MQ=255)
                                                                                                                                     cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGCCCGCCCTCTGTa              >  1:1911799/1‑150 (MQ=255)
                                                                                                                                        aaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGGCTTGATGACCGCCCTCTGTATGt           >  2:1698911/1‑150 (MQ=255)
                                                                                                                                         aGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGCCATTGATTGACTTGATGACCGCCCTCTGTATGTc          >  1:585371/1‑150 (MQ=255)
                                                                                                                                          gCCCGGAAAATCCGGGGTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCt         <  1:1924633/150‑1 (MQ=255)
                                                                                                                                          gCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTAAATTTATGATCGATATCGCATTGATTTACTTGATGACCTCCCTCTGTATGTCt         >  2:1250377/1‑150 (MQ=255)
                                                                                                                                           cccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTg        <  2:1525991/150‑1 (MQ=255)
                                                                                                                                             cGGAAAATCCGGGCTTCGCTGGTCCCTCAAGGGGAGAAAACTTAGGGCCGCTATGGGCTACAAAGTCATTATGGGGGATGGATTTTTAATTATCAATTTATGAGCGATATGGCATTCATTGACTTGATGACCGCCCTCTGTATGTCTGca      >  2:1087874/1‑150 (MQ=255)
                                                                                                                                             cGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGca      >  2:453002/1‑150 (MQ=255)
                                                                                                                                              ggAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAGTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaa     >  1:264696/1‑150 (MQ=255)
                                                                                                                                                 aaaTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaacca  >  1:1175211/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCA  >  NC_000913/3959779‑3960073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: