Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,063,571 A→G 5.9% intergenic (+59/+32) yihN → / ← ompL MFS transporter family protein/outer membrane porin L; putative sulpholipid porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,063,5710AG 5.9% 405.9 / 10.4 135intergenic (+59/+32)yihN/ompLMFS transporter family protein/outer membrane porin L; putative sulpholipid porin
Reads supporting (aligned to +/- strand):  ref base A (63/63);  new base G (2/6);  total (66/69)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 135 total aligned reads displayed.

GCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTA  >  NC_000913/4063424‑4063719
                                                                                                                                                   |                                                                                                                                                    
gCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAg                                                                                                                                                    <  2:1674161/150‑1 (MQ=255)
gCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAgg                                                                                                                                                   <  2:1974400/150‑1 (MQ=255)
  ttCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGc                                                                                                                                                  >  2:325254/1‑150 (MQ=255)
   tCGCATTCAGTGGTGGGGTGGTTTCCGTAATGCTGGTTACGCTTATTAGCCAACGTAAGAAAGAGATGCTGGCGTCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCg                                                                                                                                                 <  2:1276031/150‑1 (MQ=255)
     gCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGtt                                                                                                                                               <  2:1815683/150‑1 (MQ=255)
      cATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGttt                                                                                                                                              <  1:1607615/150‑1 (MQ=255)
       ctgcAGTGTGGCGCTGGTTGCCGTAATGCTGGTTAATCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTGAATTACTGGCCGGTTGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTCGGCCGGATAAGGCGTTTa                                                                                                                                             <  1:345264/147‑1 (MQ=255)
          cAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                          >  1:452095/1‑150 (MQ=255)
           aggggtgCGGGGGTTTCCGTAATGCTTGTTAAGCGTTTTAGCCAACGTAAGTAAGAGATGCTGGCTGCTGAATCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                         <  2:1688476/147‑1 (MQ=255)
              ggtgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                      >  2:1112652/1‑150 (MQ=255)
                 gCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                   <  1:782124/150‑1 (MQ=255)
                   ggtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                 <  1:1010190/150‑1 (MQ=255)
                    ttggtTTCCGTAATGCTGGTTACGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCTGCTTAAGCTTAATTAATGGCCGGATGTTCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                <  1:1499502/149‑1 (MQ=255)
                    gtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                <  2:1963469/150‑1 (MQ=255)
                    gtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                <  2:202878/150‑1 (MQ=255)
                     tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                               <  1:918590/150‑2 (MQ=255)
                     tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                               >  1:416050/1‑150 (MQ=255)
                     tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                               <  1:1912345/150‑1 (MQ=255)
                      ggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                                              >  1:1812608/1‑150 (MQ=255)
                      ggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCAg                                                                                                                              >  1:611063/1‑150 (MQ=255)
                         ttCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGttt                                                                                                                           >  2:468089/1‑150 (MQ=255)
                           ccGTAATGCTGGTTAAGCGTATTAGCAAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTca                                                                                                                         <  2:1310680/150‑1 (MQ=255)
                            cGTAATGCTGGTTAAGCGTATTAGCCTACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcac                                                                                                                        <  2:1361075/150‑1 (MQ=255)
                            cGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcac                                                                                                                        <  1:299246/150‑1 (MQ=255)
                                 tGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCag                                                                                                                   <  2:2011139/150‑1 (MQ=255)
                                  gCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCaga                                                                                                                  >  1:941830/1‑150 (MQ=255)
                                  gCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCaga                                                                                                                  >  1:560249/1‑150 (MQ=255)
                                     ggTTAAGCGTATTAGCTAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCagaaga                                                                                                               >  2:1797303/1‑150 (MQ=255)
                                        tAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGTCCGGATGTGCTTAATACGGCCAACGAAAACCGTATCAACCGTAGGCCGGATACGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAAt                                                                                                            >  2:1876990/1‑150 (MQ=255)
                                          aGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATAc                                                                                                          >  2:1117418/1‑150 (MQ=255)
                                            cGTATTCGCCACCGTACGCAAGAGATGCTGGCGGCTGAAGCTTAACTAATGGCCGGACGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTCTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACtt                                                                                                        <  2:107904/150‑1 (MQ=38)
                                            cGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACtt                                                                                                        <  1:1815164/150‑1 (MQ=255)
                                             gTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTc                                                                                                       >  1:130917/1‑150 (MQ=255)
                                               aTTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGt                                                                                                     >  1:908117/1‑150 (MQ=255)
                                                  aGCCAACGTAATAAAGAGATGCTGGCGGATGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTccc                                                                                                  <  1:1335225/150‑1 (MQ=255)
                                                  aGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTccc                                                                                                  >  1:941698/1‑150 (MQ=255)
                                                   gCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGGGTTTACGCCGAAGCCGGCAGTTGCCCAGCAGATGAAATAGTTCGTCCCg                                                                                                 >  2:1583377/1‑150 (MQ=255)
                                                     cAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAt                                                                                               >  2:1489640/1‑150 (MQ=255)
                                                     cAACGTAAGAAAGAGATGCTGGCGGCAGAAGCTTAATTAAAGGCCGGATGAGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTATACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAt                                                                                               >  2:975141/1‑150 (MQ=255)
                                                      atcGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATa                                                                                              <  2:446138/148‑1 (MQ=255)
                                                        cGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTTTCAACCGTAGGCCGGATGAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGTAGAAATACTGCGTCCCgcgacg                                                                                            >  2:163961/1‑145 (MQ=39)
                                                        cGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACg                                                                                            >  1:1408479/1‑150 (MQ=255)
                                                         gTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCCGATAAGGCGTTTACGCCGCCTCAGGCAGATTCACATCAGAAGAAATACTTCCTCCCGATACgg                                                                                           >  2:324342/1‑150 (MQ=255)
                                                            agaaagaGATGCTGGCGTCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATc                                                                                        <  1:1868885/150‑1 (MQ=255)
                                                            agaaagaGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATc                                                                                        <  1:307427/150‑1 (MQ=255)
                                                             gaaagaGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCt                                                                                       >  2:1523824/1‑150 (MQ=255)
                                                                agagATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGt                                                                                    <  1:781274/150‑1 (MQ=255)
                                                                  ataTGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGttt                                                                                  <  2:1830391/148‑1 (MQ=255)
                                                                    aTGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGTATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTg                                                                                <  2:13757/150‑1 (MQ=255)
                                                                        tGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTCTCAACCGTAGGCCGGATCAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTcg                                                                            >  1:107186/1‑150 (MQ=255)
                                                                            ggcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggt                                                                        <  1:926289/150‑1 (MQ=255)
                                                                             gcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGAggtg                                                                       >  2:1920363/1‑150 (MQ=255)
                                                                               tGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAATCCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggtggt                                                                     >  1:14616/1‑150 (MQ=255)
                                                                                   gCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACgg                                                                 >  1:1143820/1‑150 (MQ=255)
                                                                                    cTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTAGCGCGGGTACGGt                                                                >  2:1429888/1‑150 (MQ=255)
                                                                                         ttactGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGAc                                                           <  2:1272650/150‑1 (MQ=255)
                                                                                          taatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACg                                                          >  1:1164598/1‑150 (MQ=255)
                                                                                            atGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTGGCGGTGGTACGGTTCGACGtt                                                        >  1:191862/1‑150 (MQ=255)
                                                                                             tGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTg                                                       <  2:1659223/150‑1 (MQ=255)
                                                                                              ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                      >  2:1000492/1‑150 (MQ=255)
                                                                                              ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTAGCACCCGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCAGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                      <  2:1115818/150‑1 (MQ=255)
                                                                                               gCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCg                                                     <  1:545618/150‑1 (MQ=255)
                                                                                                ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                    <  1:907185/150‑1 (MQ=255)
                                                                                                ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                    >  1:1341322/1‑150 (MQ=255)
                                                                                                ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                    <  1:309642/150‑1 (MQ=255)
                                                                                                 cGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGt                                                   <  2:452095/150‑1 (MQ=255)
                                                                                                 cGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTACGACGTTGCGGt                                                   <  2:1075761/150‑1 (MQ=255)
                                                                                                    aTGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTa                                                <  1:1957925/150‑1 (MQ=255)
                                                                                                     tgtgCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTaa                                               <  2:388624/150‑1 (MQ=255)
                                                                                                        gCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCa                                            >  1:1585432/1‑150 (MQ=255)
                                                                                                         cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTCACCAg                                           >  2:1280142/1‑150 (MQ=255)
                                                                                                         cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAg                                           >  2:730649/1‑150 (MQ=255)
                                                                                                           tAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGCAGTTTCACTTCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAgcg                                         <  2:1357020/150‑1 (MQ=255)
                                                                                                             aTCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCa                                       >  1:536455/1‑150 (MQ=255)
                                                                                                                cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                    >  2:707401/1‑150 (MQ=255)
                                                                                                                cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                    <  2:941830/150‑1 (MQ=255)
                                                                                                                  gCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCg                                  >  2:568792/1‑150 (MQ=255)
                                                                                                                   ccAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGa                                 <  1:1261137/150‑1 (MQ=255)
                                                                                                                    cAACGAAAACCGTATCACCCGTACGACGGATACGGCGTTTACGCCGCATACGGCAGTTTCACATCAGAAGAAATACTTCCTCCCGATACTGATCTGGTTTTGCTCGCGCTGGTACCCTTCGACGTTGCGGGCTACCCAGAGCAATTCGaa                                >  2:927556/1‑150 (MQ=255)
                                                                                                                    cAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaa                                >  2:1963415/1‑150 (MQ=255)
                                                                                                                     aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                               <  1:757826/150‑1 (MQ=255)
                                                                                                                     aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                               >  1:215454/1‑150 (MQ=255)
                                                                                                                     aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                               <  1:1402026/150‑1 (MQ=255)
                                                                                                                       cGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATa                             >  1:1503564/1‑150 (MQ=255)
                                                                                                                            aCCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGa                        >  1:196830/1‑150 (MQ=255)
                                                                                                                             ccGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAg                       <  2:1344241/150‑1 (MQ=255)
                                                                                                                              cGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATAAGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGc                      >  1:1609676/1‑150 (MQ=255)
                                                                                                                                tATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAAACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCa                    <  1:102326/150‑1 (MQ=255)
                                                                                                                                 aTCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAg                   <  1:199969/150‑1 (MQ=255)
                                                                                                                                    aaCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAgtgt                >  1:220467/1‑150 (MQ=255)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTc              <  1:443839/150‑1 (MQ=255)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTc              <  1:634631/150‑1 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACCTCAGAAGAAATACTTCGGCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCAtt           <  2:784446/150‑1 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGCCCCGATACGGATCGGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCAtt           <  2:1542852/150‑1 (MQ=255)
                                                                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTaa         >  2:1479431/1‑150 (MQ=255)
                                                                                                                                            gCCGGATGCGGCGTAAACGCCTTATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAAt        >  2:1987051/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATa       >  2:16906/1‑150 (MQ=255)
                                                                                                                                              cGGATAAGGCTTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCTATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTAGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATAc      <  1:1496975/150‑1 (MQ=255)
                                                                                                                                               ggATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAGACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACg     >  2:1843900/1‑150 (MQ=255)
                                                                                                                                                  tAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTa  >  2:929115/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTA  >  NC_000913/4063424‑4063719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: