Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 399,810 A→C 5.4% intergenic (+5/+19) yaiZ → / ← ddlA DUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913399,8100AC 5.4% 296.3 / 6.1 115intergenic (+5/+19)yaiZ/ddlADUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base A (59/49);  new base C (2/5);  total (61/54)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

Only 100 of 124 total aligned reads displayed.

TCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGA  >  NC_000913/399661‑399959
                                                                                                                                                     |                                                                                                                                                     
tcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAata                                                                                                                                                       >  2:1604963/1‑150 (MQ=255)
 cTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAatat                                                                                                                                                      >  1:937472/1‑150 (MQ=255)
  ttAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTTTTCTTCGTCGCGATGAAGAACCTAAAAACGCCCAATAATAatata                                                                                                                                                     >  2:369811/1‑149 (MQ=255)
   tAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAAtatta                                                                                                                                                    >  1:1627876/1‑150 (MQ=255)
     aTGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATc                                                                                                                                                  >  1:619405/1‑150 (MQ=255)
      tGGCGTGGTGGGGTTACTGGGAGTTGAAGCAAAAGGTTGGGAGACCTATGCCGTCGGTCTGGTGACGAGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCg                                                                                                                                                 <  1:857943/150‑1 (MQ=255)
       ggCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCgg                                                                                                                                                >  1:648739/1‑150 (MQ=255)
         cGTTGTGGGTTTACTGGGATTTGCAGCAAAATGTTGGCAGACCTATGCCGTCGGTCTGGGGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGc                                                                                                                                              <  1:1516435/150‑1 (MQ=255)
          gtggtgGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGGCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCg                                                                                                                                             <  2:1543450/150‑1 (MQ=255)
            ggtggtGTTACTTGGATTTGAAGCAAAACGTTGGCAGACCTATGCCGTCGGTCTGGGGACGTGGGTGATTAGTTTCTGGCTGGTGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGtt                                                                                                                                           <  1:168595/150‑1 (MQ=255)
             gtggGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGGCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTa                                                                                                                                          <  1:1960941/150‑1 (MQ=255)
               ggggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaaa                                                                                                                                        >  2:712555/1‑150 (MQ=255)
                gggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGTTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttca                                                                                                                                       <  2:1422094/150‑5 (MQ=255)
                 ggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAat                                                                                                                                      >  1:1921270/1‑150 (MQ=255)
                  gTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttcagt                                                                                                                                     <  1:1870062/150‑7 (MQ=255)
                    tACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATAtt                                                                                                                                   >  1:1538817/1‑150 (MQ=255)
                    tACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATAtt                                                                                                                                   >  1:1805430/1‑150 (MQ=255)
                        gggATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACAt                                                                                                                               >  2:1508756/1‑150 (MQ=255)
                        gggATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACAt                                                                                                                               >  2:1932998/1‑150 (MQ=255)
                        gggATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACAt                                                                                                                               >  1:943215/1‑150 (MQ=255)
                          gATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATtg                                                                                                                             >  1:1032303/1‑150 (MQ=255)
                             ttGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                          <  2:89178/150‑1 (MQ=255)
                             ttGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                          >  1:1910844/1‑150 (MQ=255)
                               gAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGtt                                                                                                                        >  2:1919943/1‑150 (MQ=255)
                               gAAGCAAAAGGTTGCCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTATCTGGCTGGCGGGGTTGATTATTCGTCGTCGAGATCAAGAAACTGAAAACGCCCAATAAGAATATTATCGGTCGTTAAAATATTACATTGTGGtt                                                                                                                        >  2:153037/1‑150 (MQ=255)
                                 aGCAAAAGGTTGGCAGACCTATGCCGTCTGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGtttt                                                                                                                      <  1:1447577/150‑1 (MQ=255)
                                 aGCAAAAGGTTGGCAGACCTATGACGTCCGTCTGGAGACGTCGGTGATTAGTTTCTGGCTGGCGGGGCTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGtttt                                                                                                                      >  2:1949377/1‑150 (MQ=255)
                                  gccacaGGTTGGCAGAACTATTCCGTCGGTCTGGTGACGTGGGTGATTAGTTGCTGGCTGGCGGGGGTGAGTAGTCGTCGTCGCGATGCAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                     <  2:116463/145‑1 (MQ=255)
                                  gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                     >  2:887555/1‑150 (MQ=255)
                                  gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                     >  2:796949/1‑150 (MQ=255)
                                  gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                     <  1:1572936/150‑1 (MQ=255)
                                   cAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCa                                                                                                                    >  1:1498031/1‑150 (MQ=255)
                                    aaaaGGTTGGCAGACCTATGCAGACGGTCTGGGGAAGTGGGTGATTAGTTTCTGGCTGGCGGGTTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGACCATTAAGAATATTATCGGGCTTTAAAATATTACATTGTGGTTTTCaa                                                                                                                   >  1:1231099/1‑150 (MQ=255)
                                     aaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAAt                                                                                                                  >  2:1313840/1‑150 (MQ=255)
                                       aGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGc                                                                                                                <  2:608301/150‑1 (MQ=255)
                                       aGGTGGGCAGACCTATGCCGTCGGTCTGGGGACGTGGGTGATTAGTTGCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACTCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGc                                                                                                                <  2:257948/150‑1 (MQ=255)
                                        ggTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCg                                                                                                               >  1:549098/1‑150 (MQ=255)
                                        gattGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCg                                                                                                               >  2:1344801/3‑150 (MQ=255)
                                         gTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                              <  2:1587232/150‑1 (MQ=255)
                                          ttGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGtt                                                                                                             <  1:1095915/150‑1 (MQ=255)
                                              cAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATcc                                                                                                         >  1:695314/1‑150 (MQ=255)
                                                   cTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGc                                                                                                    >  2:863643/1‑150 (MQ=255)
                                                    tATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGca                                                                                                   >  2:561407/1‑149 (MQ=255)
                                                    tATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCg                                                                                                   <  1:1659982/150‑1 (MQ=255)
                                                      tGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTg                                                                                                 >  1:674713/1‑150 (MQ=255)
                                                                cTGGTGACGTGGGTGACTAGTTTCTGGCGGGCGGGGTGGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATTAGAATATTATCGGGCGTTAAAATATTCCATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAgc                                                                                       <  1:1212077/150‑1 (MQ=255)
                                                                  ggTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGCCGCTCCAgcgc                                                                                     >  1:1280129/1‑150 (MQ=255)
                                                                  ggTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAgcgc                                                                                     >  2:1137941/1‑150 (MQ=255)
                                                                       cGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGGAGCGTGACGCTCCAGCGCCAGtt                                                                                <  2:1159993/150‑1 (MQ=255)
                                                                        gTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtc                                                                               >  1:1951793/1‑150 (MQ=255)
                                                                          gggCGATTAGTTACTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGACACTGAAAACGCCCAATAAGAATCATATCGGGCGTTAAAATATTACATTGTGGTGTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaa                                                                             <  2:192030/150‑1 (MQ=255)
                                                                           ggTGATTAGTTTCTGGCTGCCGGGGTAGATTCTTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTAGCGGGCGTTAATATATTACATTGTGGCTTTCAATTCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaat                                                                            <  2:314558/150‑1 (MQ=255)
                                                                            gTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatc                                                                           <  1:738293/150‑1 (MQ=255)
                                                                            gTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTACAGCGCCAGTtcaata                                                                           >  1:195485/1‑149 (MQ=255)
                                                                               aTTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCaaa                                                                        >  1:408646/1‑150 (MQ=255)
                                                                                 tAGTTTCTGGCTGGCGGGGTTTATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACg                                                                      <  2:1841154/150‑1 (MQ=255)
                                                                                  aGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACgt                                                                     <  1:887728/150‑1 (MQ=255)
                                                                                   gTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCTCAGCGTGACGCGACAGCGCCAGTTCAATCAAACgtg                                                                    >  2:289388/1‑150 (MQ=255)
                                                                                    tttCTGGCTGGCGGGGTTGATTATTCGTCTTCGCGATGAAGAAACTGAAAACGCGAAATAAGAATATTATCGGGCGTTGAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCACCAGTTCAATCAAACgtgt                                                                   <  2:377343/150‑1 (MQ=255)
                                                                                     ttCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGtgtg                                                                  <  2:1627876/150‑1 (MQ=255)
                                                                                      tCTGGCTGGCGGGGGTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGGGa                                                                 <  2:1794208/150‑1 (MQ=255)
                                                                                       ctggctggCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATGGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGAt                                                                <  1:430738/150‑1 (MQ=255)
                                                                                        tggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATc                                                               <  1:671909/150‑1 (MQ=255)
                                                                                        tggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATc                                                               >  1:1276097/1‑150 (MQ=255)
                                                                                         ggctggcGGGGTTGATTATTCGTCGTCGCGATTAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGTTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                              <  1:394353/150‑1 (MQ=255)
                                                                                         ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                              >  2:1036235/1‑150 (MQ=255)
                                                                                         ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                              >  2:1424644/1‑150 (MQ=255)
                                                                                           ctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGa                                                            <  2:1131787/150‑1 (MQ=255)
                                                                                             ggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATc                                                          <  1:879007/150‑1 (MQ=255)
                                                                                               cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCgg                                                        >  1:1145733/1‑150 (MQ=255)
                                                                                               cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCgg                                                        <  2:738643/150‑1 (MQ=255)
                                                                                                  ggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGgtgt                                                     >  2:813474/1‑150 (MQ=255)
                                                                                                  ggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGAGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGgtgt                                                     >  1:290547/1‑150 (MQ=255)
                                                                                                   gTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTa                                                    >  2:920232/1‑150 (MQ=255)
                                                                                                       attattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAccc                                                >  1:1256401/1‑150 (MQ=255)
                                                                                                       attattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAccc                                                >  2:1796315/1‑150 (MQ=255)
                                                                                                         tattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCCGTTCAATCACACGTGTGATCAGATCGGTGTAACCCAg                                              >  1:936321/1‑150 (MQ=255)
                                                                                                         tattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAg                                              >  1:528851/1‑150 (MQ=255)
                                                                                                          attCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGa                                             >  1:569695/1‑150 (MQ=255)
                                                                                                               tcgtcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCt                                        >  2:437252/1‑150 (MQ=255)
                                                                                                                 gtcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTgg                                      <  2:674713/150‑1 (MQ=255)
                                                                                                                  tcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGc                                     <  2:1642435/150‑1 (MQ=255)
                                                                                                                   cgcgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCt                                    <  2:1538817/150‑1 (MQ=255)
                                                                                                                     cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                  >  1:1663254/1‑150 (MQ=255)
                                                                                                                     cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                  <  2:793574/150‑1 (MQ=255)
                                                                                                                       aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                                <  1:796949/150‑1 (MQ=255)
                                                                                                                        tGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCca                               >  2:247453/1‑150 (MQ=255)
                                                                                                                         gaagaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCTCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCcac                              <  1:1498054/150‑1 (MQ=255)
                                                                                                                           agaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAg                            <  1:369811/150‑1 (MQ=255)
                                                                                                                           agaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAg                            >  1:1421090/1‑150 (MQ=255)
                                                                                                                            gaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGc                           >  2:293440/1‑150 (MQ=255)
                                                                                                                              aaCTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCtt                         >  1:412641/1‑150 (MQ=255)
                                                                                                                               aCTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTc                        <  2:386416/150‑1 (MQ=255)
                                                                                                                                attttaaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGTTGTAACCCAGACCGCTGGCTTGCCACAGCTTCg                       >  2:487777/6‑150 (MQ=255)
                                                                                                                                   aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATTTTACATTGTTGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGCCCGCTGGCTTGCCACAGCTGCGGat                    <  2:815770/150‑1 (MQ=255)
                                                                                                                                       cGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacat                >  1:1326873/1‑150 (MQ=255)
                                                                                                                                       cGCCCAATAAGATTATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacat                <  2:363165/150‑1 (MQ=255)
                                                                                                                                         cccAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacatac              >  2:430289/1‑150 (MQ=255)
                                                                                                                                                  aaTATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAg     <  2:1760195/150‑1 (MQ=255)
                                                                                                                                                   atatTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGt    >  1:1720579/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGA  >  NC_000913/399661‑399959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: