Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,063,572 A→C 6.1% intergenic (+60/+31) yihN → / ← ompL MFS transporter family protein/outer membrane porin L; putative sulpholipid porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,063,5720AC 6.1% 395.6 / 10.4 135intergenic (+60/+31)yihN/ompLMFS transporter family protein/outer membrane porin L; putative sulpholipid porin
Reads supporting (aligned to +/- strand):  ref base A (63/63);  new base C (3/6);  total (66/69)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAAC  >  NC_000913/4063424‑4063721
                                                                                                                                                    |                                                                                                                                                     
gCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAgg                                                                                                                                                     <  2:1974400/150‑1 (MQ=255)
  ttCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGc                                                                                                                                                    >  2:325254/1‑150 (MQ=255)
   tCGCATTCAGTGGTGGGGTGGTTTCCGTAATGCTGGTTACGCTTATTAGCCAACGTAAGAAAGAGATGCTGGCGTCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCg                                                                                                                                                   <  2:1276031/150‑1 (MQ=255)
     gCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                 >  1:784446/1‑150 (MQ=255)
     gCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                 <  2:1815683/150‑1 (MQ=255)
           cgtggtgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  2:1685818/149‑1 (MQ=255)
           aggggtgCGGGGGTTTCCGTAATGCTTGTTAAGCGTTTTAGCCAACGTAAGTAAGAGATGCTGGCTGCTGAATCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  2:1688476/147‑1 (MQ=255)
           aGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  2:1866983/150‑1 (MQ=255)
           aGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  1:1718664/150‑1 (MQ=255)
              ggtgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                        >  2:1112652/1‑150 (MQ=255)
                tgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                      >  2:193225/1‑150 (MQ=255)
                   ggtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                   <  1:1010190/150‑1 (MQ=255)
                    ttggtTTCCGTAATGCTGGTTACGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCTGCTTAAGCTTAATTAATGGCCGGATGTTCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                  <  1:1499502/149‑1 (MQ=255)
                    gtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                  <  2:202878/150‑1 (MQ=255)
                     tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                 <  1:918590/150‑2 (MQ=255)
                     tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                 >  1:416050/1‑150 (MQ=255)
                      ggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                                                >  1:1812608/1‑150 (MQ=255)
                      ggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCAg                                                                                                                                >  1:611063/1‑150 (MQ=255)
                         ttCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGttt                                                                                                                             >  2:468089/1‑150 (MQ=255)
                           ccGTAATGCTGGTTAAGCGTATTAGCAAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTca                                                                                                                           <  2:1310680/150‑1 (MQ=255)
                            cGTAATGCTGGTTAAGCGTATTAGCCTACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcac                                                                                                                          <  2:1361075/150‑1 (MQ=255)
                            cGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcac                                                                                                                          >  1:1342380/1‑150 (MQ=255)
                             gTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcaca                                                                                                                         <  1:918804/150‑1 (MQ=255)
                                  gCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCaga                                                                                                                    >  1:941830/1‑150 (MQ=255)
                                  gCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCaga                                                                                                                    >  1:560249/1‑150 (MQ=255)
                                    tGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCagaag                                                                                                                  <  2:1986958/150‑1 (MQ=255)
                                     ggTTAAGCGTATTAGCTAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCagaaga                                                                                                                 >  2:1797303/1‑150 (MQ=255)
                                          aGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATAc                                                                                                            >  2:1117418/1‑150 (MQ=255)
                                            cGTATTCGCCACCGTACGCAAGAGATGCTGGCGGCTGAAGCTTAACTAATGGCCGGACGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTCTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACtt                                                                                                          <  2:107904/150‑1 (MQ=38)
                                             gTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTc                                                                                                         >  1:130917/1‑150 (MQ=255)
                                                  aGCCAACGTAATAAAGAGATGCTGGCGGATGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTccc                                                                                                    <  1:1335225/150‑1 (MQ=255)
                                                  aGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTccc                                                                                                    >  1:941698/1‑150 (MQ=255)
                                                     cAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAt                                                                                                 <  1:468089/150‑1 (MQ=255)
                                                     cAACGTAAGAAAGAGATGCTGGCGGCAGAAGCTTAATTAAAGGCCGGATGAGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTATACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAt                                                                                                 >  2:975141/1‑150 (MQ=255)
                                                        cGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTTTCAACCGTAGGCCGGATGAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGTAGAAATACTGCGTCCCgcgacg                                                                                              >  2:163961/1‑145 (MQ=39)
                                                        cGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACg                                                                                              >  1:1408479/1‑150 (MQ=255)
                                                            agaaagaGATGCTGGCGTCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATc                                                                                          <  1:1868885/150‑1 (MQ=255)
                                                            agaaagaGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGAtg                                                                                          >  1:1855018/1‑149 (MQ=255)
                                                            agaaagaGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATc                                                                                          <  1:307427/150‑1 (MQ=255)
                                                             gaaagaGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCt                                                                                         >  2:1523824/1‑150 (MQ=255)
                                                                agagATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGt                                                                                      <  1:781274/150‑1 (MQ=255)
                                                                    aTGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGTATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTg                                                                                  <  2:13757/150‑1 (MQ=255)
                                                                     tGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGc                                                                                 >  2:1519760/1‑150 (MQ=255)
                                                                      gCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCt                                                                                <  2:509115/150‑1 (MQ=255)
                                                                        tGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTCTCAACCGTAGGCCGGATCAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTcg                                                                              >  1:107186/1‑150 (MQ=255)
                                                                           cggcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCgg                                                                           <  2:416050/150‑1 (MQ=255)
                                                                            ggcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggt                                                                          <  1:926289/150‑1 (MQ=255)
                                                                            ggcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggt                                                                          <  1:306381/150‑1 (MQ=255)
                                                                             gcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGAggtg                                                                         >  2:1920363/1‑150 (MQ=255)
                                                                                gAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTa                                                                      >  2:1291179/1‑150 (MQ=255)
                                                                                   gCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACgg                                                                   >  1:1143820/1‑150 (MQ=255)
                                                                                    cTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTAGCGCGGGTACGGt                                                                  >  2:1429888/1‑150 (MQ=255)
                                                                                     ttaattaaTGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGtt                                                                 >  2:1808639/1‑150 (MQ=255)
                                                                                        attaatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGa                                                              >  1:388672/1‑150 (MQ=255)
                                                                                         ttactGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGAc                                                             <  2:1272650/150‑1 (MQ=255)
                                                                                          taatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACg                                                            >  1:1164598/1‑150 (MQ=255)
                                                                                           aatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGt                                                           <  1:737139/150‑1 (MQ=255)
                                                                                            atGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTGGCGGTGGTACGGTTCGACGtt                                                          >  1:191862/1‑150 (MQ=255)
                                                                                             tGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTg                                                         <  2:1659223/150‑1 (MQ=255)
                                                                                              ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                        <  2:1627019/150‑1 (MQ=255)
                                                                                              ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                        >  2:1000492/1‑150 (MQ=255)
                                                                                              ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTAGCACCCGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCAGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                        <  2:1115818/150‑1 (MQ=255)
                                                                                                ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                      <  1:907185/150‑1 (MQ=255)
                                                                                                ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                      <  1:309642/150‑1 (MQ=255)
                                                                                                 cGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGt                                                     <  2:452095/150‑1 (MQ=255)
                                                                                                 cGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTACGACGTTGCGGt                                                     <  2:1075761/150‑1 (MQ=255)
                                                                                                  ggATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTc                                                    <  1:1467187/150‑1 (MQ=255)
                                                                                                     tgtgCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTaa                                                 <  2:388624/150‑1 (MQ=255)
                                                                                                      gtgCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAAc                                                >  1:2003754/1‑150 (MQ=255)
                                                                                                      gtgCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCAGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAAc                                                >  2:32341/1‑150 (MQ=255)
                                                                                                        gCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCa                                              >  1:1585432/1‑150 (MQ=255)
                                                                                                         cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAg                                             >  1:1563723/1‑150 (MQ=255)
                                                                                                         cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAg                                             >  2:730649/1‑150 (MQ=255)
                                                                                                           tAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGCAGTTTCACTTCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAgcg                                           <  2:1357020/150‑1 (MQ=255)
                                                                                                             aTCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCa                                         >  1:536455/1‑150 (MQ=255)
                                                                                                                cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                      >  2:707401/1‑150 (MQ=255)
                                                                                                                cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                      <  2:941830/150‑1 (MQ=255)
                                                                                                                cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTAGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                      >  1:1919711/1‑150 (MQ=255)
                                                                                                                   ccAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGa                                   <  1:1261137/150‑1 (MQ=255)
                                                                                                                    cAACGAAAACCGTATCACCCGTACGACGGATACGGCGTTTACGCCGCATACGGCAGTTTCACATCAGAAGAAATACTTCCTCCCGATACTGATCTGGTTTTGCTCGCGCTGGTACCCTTCGACGTTGCGGGCTACCCAGAGCAATTCGaa                                  >  2:927556/1‑150 (MQ=255)
                                                                                                                    cAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaa                                  >  2:1963415/1‑150 (MQ=255)
                                                                                                                     aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                                 <  1:1402026/150‑1 (MQ=255)
                                                                                                                     aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                                 <  1:757826/150‑1 (MQ=255)
                                                                                                                            aCCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGa                          >  1:196830/1‑150 (MQ=255)
                                                                                                                             ccGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAg                         <  2:1344241/150‑1 (MQ=255)
                                                                                                                              cGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATAAGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGc                        >  1:1609676/1‑150 (MQ=255)
                                                                                                                                tATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAAACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCa                      <  1:102326/150‑1 (MQ=255)
                                                                                                                                 aTCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAg                     <  1:199969/150‑1 (MQ=255)
                                                                                                                                    aaCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAgtgt                  >  1:220467/1‑150 (MQ=255)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTc                <  1:443839/150‑1 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACCTCAGAAGAAATACTTCGGCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCAtt             <  2:784446/150‑1 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGCCCCGATACGGATCGGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCAtt             <  2:1542852/150‑1 (MQ=255)
                                                                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTaa           >  2:1479431/1‑150 (MQ=255)
                                                                                                                                            gCCGGATGCGGCGTAAACGCCTTATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAAt          >  2:1987051/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATa         <  1:568792/150‑1 (MQ=255)
                                                                                                                                              cGGATAAGGCTTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCTATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTAGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATAc        <  1:1496975/150‑1 (MQ=255)
                                                                                                                                               ggATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAGACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACg       >  2:1843900/1‑150 (MQ=255)
                                                                                                                                                  tAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTa    >  2:929115/1‑150 (MQ=255)
                                                                                                                                                    aGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGTTTTTGCTCGCGTTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCTAAATAAGTGAGCCAGTGTTCATTAATACGGTAAc  <  1:1876990/150‑1 (MQ=255)
                                                                                                                                                    aGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAAc  <  1:258351/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAAC  >  NC_000913/4063424‑4063721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: