Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,063,578 T→A 6.0% intergenic (+66/+25) yihN → / ← ompL MFS transporter family protein/outer membrane porin L; putative sulpholipid porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,063,5780TA 6.0% 388.4 / 9.4 132intergenic (+66/+25)yihN/ompLMFS transporter family protein/outer membrane porin L; putative sulpholipid porin
Reads supporting (aligned to +/- strand):  ref base T (64/59);  new base A (2/6);  total (66/66)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 136 total aligned reads displayed.

GCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAACGGAAGG  >  NC_000913/4063429‑4063727
                                                                                                                                                     |                                                                                                                                                     
gCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                       >  1:784446/1‑150 (MQ=255)
gCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                       <  2:1815683/150‑1 (MQ=255)
 cATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGttt                                                                                                                                                      <  1:1607615/150‑1 (MQ=255)
  ctgcAGTGTGGCGCTGGTTGCCGTAATGCTGGTTAATCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTGAATTACTGGCCGGTTGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTCGGCCGGATAAGGCGTTTa                                                                                                                                                     <  1:345264/147‑1 (MQ=255)
     cAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                  >  1:452095/1‑150 (MQ=255)
      cgtggtgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                 <  2:1685818/149‑1 (MQ=255)
      aggggtgCGGGGGTTTCCGTAATGCTTGTTAAGCGTTTTAGCCAACGTAAGTAAGAGATGCTGGCTGCTGAATCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                 <  2:1688476/147‑1 (MQ=255)
      aGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                 <  2:1866983/150‑1 (MQ=255)
      aGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                 <  1:1718664/150‑1 (MQ=255)
         ggtgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                              >  2:1112652/1‑150 (MQ=255)
           tgCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                            >  2:193225/1‑150 (MQ=255)
            gCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                           <  1:782124/150‑1 (MQ=255)
              ggtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                         <  1:1010190/150‑1 (MQ=255)
               ttggtTTCCGTAATGCTGGTTACGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCTGCTTAAGCTTAATTAATGGCCGGATGTTCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                        <  1:1499502/149‑1 (MQ=255)
               gtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                        <  2:1963469/150‑1 (MQ=255)
               gtggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                        <  2:202878/150‑1 (MQ=255)
                tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                       <  1:918590/150‑2 (MQ=255)
                tggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                       <  1:1912345/150‑1 (MQ=255)
                 ggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                                                      >  1:1812608/1‑150 (MQ=255)
                 ggtTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCAg                                                                                                                                      >  1:611063/1‑150 (MQ=255)
                    ttCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGttt                                                                                                                                   >  2:468089/1‑150 (MQ=255)
                      ccGTAATGCTGGTTAAGCGTATTAGCAAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTca                                                                                                                                 <  2:1310680/150‑1 (MQ=255)
                       cGTAATGCTGGTTAAGCGTATTAGCCTACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcac                                                                                                                                <  2:1361075/150‑1 (MQ=255)
                       cGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcac                                                                                                                                <  1:299246/150‑1 (MQ=255)
                        gTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTcaca                                                                                                                               <  1:918804/150‑1 (MQ=255)
                            tGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCag                                                                                                                           <  2:2011139/150‑1 (MQ=255)
                             gCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCaga                                                                                                                          >  1:560249/1‑150 (MQ=255)
                                ggTTAAGCGTATTAGCTAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCagaaga                                                                                                                       >  2:1797303/1‑150 (MQ=255)
                                   tAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGTCCGGATGTGCTTAATACGGCCAACGAAAACCGTATCAACCGTAGGCCGGATACGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAAt                                                                                                                    >  2:1876990/1‑150 (MQ=255)
                                       cGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACtt                                                                                                                <  1:1815164/150‑1 (MQ=255)
                                        gTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTc                                                                                                               >  1:130917/1‑150 (MQ=255)
                                          aTTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGt                                                                                                             >  1:908117/1‑150 (MQ=255)
                                             aGCCAACGTAATAAAGAGATGCTGGCGGATGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTccc                                                                                                          <  1:1335225/150‑1 (MQ=255)
                                             aGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTccc                                                                                                          >  1:941698/1‑150 (MQ=255)
                                                cAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAt                                                                                                       <  1:468089/150‑1 (MQ=255)
                                                cAACGTAAGAAAGAGATGCTGGCGGCAGAAGCTTAATTAAAGGCCGGATGAGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTATACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAt                                                                                                       >  2:975141/1‑150 (MQ=255)
                                                 atcGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATa                                                                                                      <  2:446138/148‑1 (MQ=255)
                                                   cGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTTTCAACCGTAGGCCGGATGAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGTAGAAATACTGCGTCCCgcgacg                                                                                                    >  2:163961/1‑145 (MQ=39)
                                                   cGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACg                                                                                                    >  1:1408479/1‑150 (MQ=255)
                                                    gTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCCGATAAGGCGTTTACGCCGCCTCAGGCAGATTCACATCAGAAGAAATACTTCCTCCCGATACgg                                                                                                   >  2:324342/1‑150 (MQ=255)
                                                       agaaagaGATGCTGGCGTCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATc                                                                                                <  1:1868885/150‑1 (MQ=255)
                                                        gaaagaGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCt                                                                                               >  2:1523824/1‑150 (MQ=255)
                                                           agagATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGt                                                                                            <  1:781274/150‑1 (MQ=255)
                                                           agagATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTGAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGt                                                                                            <  2:1250263/150‑1 (MQ=255)
                                                               aTGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGTATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTg                                                                                        <  2:13757/150‑1 (MQ=255)
                                                                tGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGc                                                                                       >  2:1519760/1‑150 (MQ=255)
                                                                   tGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTCTCAACCGTAGGCCGGATCAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTcg                                                                                    >  1:107186/1‑150 (MQ=255)
                                                                       ggcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggt                                                                                <  1:306381/150‑1 (MQ=255)
                                                                        gcTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggtg                                                                               <  2:1420293/150‑1 (MQ=255)
                                                                          tGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAATCCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCggtggt                                                                             >  1:14616/1‑150 (MQ=255)
                                                                              gCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACgg                                                                         >  1:1143820/1‑150 (MQ=255)
                                                                               cTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTAGCGCGGGTACGGt                                                                        >  2:1429888/1‑150 (MQ=255)
                                                                                ttaattaaTGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGtt                                                                       >  2:1808639/1‑150 (MQ=255)
                                                                                   attaatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGa                                                                    >  1:388672/1‑150 (MQ=255)
                                                                                    ttactGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGAc                                                                   <  2:1272650/150‑1 (MQ=255)
                                                                                     taatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACg                                                                  >  1:1164598/1‑150 (MQ=255)
                                                                                      aatGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGt                                                                 <  1:737139/150‑1 (MQ=255)
                                                                                        tGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTg                                                               <  2:1659223/150‑1 (MQ=255)
                                                                                         ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                              <  2:1627019/150‑1 (MQ=255)
                                                                                         ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                              >  2:1000492/1‑150 (MQ=255)
                                                                                         ggCCGGATGTGCTTAATCCGGCCAACGAAAACCGTAGCACCCGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCAGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGc                                                              <  2:1115818/150‑1 (MQ=255)
                                                                                          gCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCg                                                             <  1:545618/150‑1 (MQ=255)
                                                                                           ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                            <  1:309642/150‑1 (MQ=255)
                                                                                           ccGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCgg                                                            >  1:1341322/1‑150 (MQ=255)
                                                                                            cGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGt                                                           <  2:452095/150‑1 (MQ=255)
                                                                                            cGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTACGACGTTGCGGt                                                           <  2:1075761/150‑1 (MQ=255)
                                                                                               aTGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTa                                                        <  1:1957925/150‑1 (MQ=255)
                                                                                                tgtgCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTaa                                                       <  2:388624/150‑1 (MQ=255)
                                                                                                 gtgCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAAc                                                      >  1:2003754/1‑150 (MQ=255)
                                                                                                   gCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCa                                                    >  1:1585432/1‑150 (MQ=255)
                                                                                                   gCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCa                                                    >  2:1088408/1‑150 (MQ=255)
                                                                                                    cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTCACCAg                                                   >  2:1280142/1‑150 (MQ=255)
                                                                                                    cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAg                                                   >  2:730649/1‑150 (MQ=255)
                                                                                                    cTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAg                                                   >  1:1563723/1‑150 (MQ=255)
                                                                                                           cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                            <  2:941830/150‑1 (MQ=255)
                                                                                                           cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                            >  2:707401/1‑150 (MQ=255)
                                                                                                           cGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTAGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAAtt                                            >  1:1919711/1‑150 (MQ=255)
                                                                                                             gCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCg                                          >  2:568792/1‑150 (MQ=255)
                                                                                                              ccAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGa                                         <  1:1261137/150‑1 (MQ=255)
                                                                                                               cAACGAAAACCGTATCACCCGTACGACGGATACGGCGTTTACGCCGCATACGGCAGTTTCACATCAGAAGAAATACTTCCTCCCGATACTGATCTGGTTTTGCTCGCGCTGGTACCCTTCGACGTTGCGGGCTACCCAGAGCAATTCGaa                                        >  2:927556/1‑150 (MQ=255)
                                                                                                               cAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaa                                        >  2:1963415/1‑150 (MQ=255)
                                                                                                                aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                                       <  1:1402026/150‑1 (MQ=255)
                                                                                                                aaCGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGaaa                                       >  1:215454/1‑150 (MQ=255)
                                                                                                                   gAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTATCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATaa                                    >  1:962178/1‑150 (MQ=255)
                                                                                                                        ccGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAg                               <  2:1344241/150‑1 (MQ=255)
                                                                                                                           tATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAAACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCa                            <  1:102326/150‑1 (MQ=255)
                                                                                                                            aTCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAg                           <  1:199969/150‑1 (MQ=255)
                                                                                                                              cAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGCCGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAgtg                         >  2:1350906/1‑150 (MQ=255)
                                                                                                                                 ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTc                      <  1:634631/150‑1 (MQ=255)
                                                                                                                                 ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTc                      <  1:443839/150‑1 (MQ=255)
                                                                                                                                    tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACCTCAGAAGAAATACTTCGGCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCAtt                   <  2:784446/150‑1 (MQ=255)
                                                                                                                                    tAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGCCCCGATACGGATCGGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCAtt                   <  2:1542852/150‑1 (MQ=255)
                                                                                                                                      ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTaa                 >  2:1479431/1‑150 (MQ=255)
                                                                                                                                       gCCGGATGCGGCGTAAACGCCTTATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAAt                >  2:1987051/1‑150 (MQ=255)
                                                                                                                                         cGGATAAGGCTTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCTATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTAGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATAc              <  1:1496975/150‑1 (MQ=255)
                                                                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGAGACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACg             >  2:1843900/1‑150 (MQ=255)
                                                                                                                                             tAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTa          >  2:929115/1‑150 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGTTTTTGCTCGCGTTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCTAAATAAGTGAGCCAGTGTTCATTAATACGGTAAc        <  1:1876990/150‑1 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAAc        >  1:934479/1‑150 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAAc        <  1:258351/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTTCACATCAGAAGAAATACTTCGTCCCGATACGGATCTGGTTTTGCTCGCGGTGGTACGGTTCGACGTTGCGGTCTAACCAGCGCAATTCGAAATAAGGGAGCCAGTGTTCATTAATACGGTAACGGAAGG  >  NC_000913/4063429‑4063727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: