Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,118,831 C→A 5.1% intergenic (+71/+14) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,118,8310CA 5.1% 396.0 / 8.7 136intergenic (+71/+14)zapB/rraAFtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
Reads supporting (aligned to +/- strand):  ref base C (57/72);  new base A (2/5);  total (59/77)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 137 total aligned reads displayed.

GAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGC  >  NC_000913/4118682‑4118973
                                                                                                                                                     |                                                                                                                                              
gaACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCACCAGGTAACGCAGAAAAAAGGCACCTTGc                                                                                                                                                >  2:136451/1‑150 (MQ=255)
     acCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGc                                                                                                                                           >  1:223693/1‑150 (MQ=255)
     acCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGc                                                                                                                                           >  2:2007426/1‑150 (MQ=255)
     acCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGc                                                                                                                                           <  2:1926661/150‑1 (MQ=255)
      ccATCTGAAAGACCAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGcc                                                                                                                                          <  2:1612857/150‑1 (MQ=255)
      ccATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGcc                                                                                                                                          <  2:1604462/150‑1 (MQ=255)
      ccATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGcc                                                                                                                                          <  2:1540644/150‑1 (MQ=255)
      ccATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCAGGGACGAGGTCTGATTACGCTTCATCTACCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGCCACCTTGCGGTGcc                                                                                                                                          <  1:396151/150‑1 (MQ=255)
        aTCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCtt                                                                                                                                        <  2:599491/150‑1 (MQ=255)
         tCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttt                                                                                                                                       <  2:1925039/150‑1 (MQ=255)
          cTGAAAGAACAGCAGATCGGCGGGCAGGAACTTCTGCAGGCCCTGCTGGGTCGCATTGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGAAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                      <  1:304331/150‑1 (MQ=255)
          cTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                      >  2:1641974/1‑150 (MQ=255)
            gAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGACAACAGGTAACGCAGAAAAAAGGCACCTAGCGGTGCCTTTCtt                                                                                                                                    >  2:955224/1‑150 (MQ=255)
             aaaGAACAGCAGAACGGCTGGCAGGAACGTCTGCTGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTTCCTTTCTTa                                                                                                                                   <  1:1535302/150‑1 (MQ=255)
              aaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAt                                                                                                                                  <  1:1896786/150‑1 (MQ=255)
               aGAACAGCCGCACGGCTGGCCGCCACGGCTGCAGGCCCCGCTGGGTCGCCTGGAAGAGTTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttctg                                                                                                                                 <  2:1241144/150‑4 (MQ=255)
               aGAACAGCAGACCGTCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCAGGGAAGAGGGCTGCTTCCGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttctg                                                                                                                                 <  2:1234868/150‑4 (MQ=255)
               aGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttctg                                                                                                                                 <  1:755646/150‑4 (MQ=255)
                gAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAACGCACCTTGCGGTGCCTTTCTTTTCa                                                                                                                                >  2:555232/1‑150 (MQ=255)
                gAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                                                                                                                <  1:1021062/150‑1 (MQ=255)
                 aaCAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGAAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                                                                                                               >  1:1426140/1‑150 (MQ=255)
                    agcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCGTttcattca                                                                                                                            >  2:1466090/1‑150 (MQ=255)
                    agcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTAAGCTTCATCTTCCGCGTAATATCTTTACTGTGTAGGCCAACAGATAACTAAGAAAAAATGCACCTTGCGGTGCCTTTAGTATCAtaaa                                                                                                                            >  2:387107/1‑147 (MQ=255)
                     gcagcaCTGCGGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGGCTGATTACGCGTCATCTTCCGCGGAATTTCTTTACTGTGTAGGCCAACAGGGAACGCAGAAAAAAGGCACCTTGCGGTGCCGTTCTTATCATTCaa                                                                                                                           <  1:1059061/150‑1 (MQ=255)
                     gcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                                                                                                                           <  2:908574/150‑1 (MQ=255)
                     gcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                                                                                                                           >  1:32279/1‑150 (MQ=255)
                      cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCGGGGTCGCATGGAAGAGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                                                                                                                          <  2:102144/150‑1 (MQ=255)
                        gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCATGCGGTGCCTTTATTTTCATTCAatat                                                                                                                        >  2:1793122/1‑150 (MQ=255)
                         aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATc                                                                                                                       <  2:247227/150‑1 (MQ=255)
                           cGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                                                                                                                     >  2:1819537/1‑150 (MQ=255)
                             gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                                                                                                                   >  1:609369/1‑150 (MQ=255)
                              cTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGAGGTGCCTTTCTTATCATTCAATATCCAGCg                                                                                                                  >  2:1246086/1‑150 (MQ=255)
                               tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                                                                                                                 <  1:1257166/150‑1 (MQ=255)
                                ggCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGa                                                                                                                >  1:853791/1‑150 (MQ=255)
                                  cAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                                                                                                              >  2:664216/1‑150 (MQ=255)
                                     gAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttc                                                                                                           <  1:1845837/150‑1 (MQ=255)
                                      aaCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct                                                                                                          <  1:607039/150‑1 (MQ=255)
                                      aaCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct                                                                                                          <  1:1820187/150‑1 (MQ=255)
                                       aCGTCTGCATGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTg                                                                                                         <  2:1639711/150‑1 (MQ=255)
                                       aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTg                                                                                                         <  1:457864/150‑1 (MQ=255)
                                       aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTg                                                                                                         <  2:1934394/150‑1 (MQ=255)
                                        cGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGa                                                                                                        >  2:1300278/1‑150 (MQ=255)
                                           cTGCATGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGTCAAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                                                                                                     <  1:1812813/150‑1 (MQ=255)
                                           cTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                                                                                                     >  2:1657960/1‑150 (MQ=255)
                                            tGCAGGCCCTGCTGGGTCGGATGGAAGAGGTCTGATTACGCTTCAGCTTCCTCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTTAAAGa                                                                                                    <  1:287854/150‑1 (MQ=255)
                                             gCAGGCCCTGCTGTGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa                                                                                                   <  1:1093484/150‑1 (MQ=255)
                                               aGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata                                                                                                 >  1:1616786/1‑150 (MQ=255)
                                                  cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATAATTCAATATCCAGCGGATCTTCTGAAAGAATAATc                                                                                              >  2:420997/1‑150 (MQ=255)
                                                     tgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCg                                                                                           <  2:1763498/150‑1 (MQ=255)
                                                      gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCgg                                                                                          <  2:1977804/150‑1 (MQ=255)
                                                      gctgGGTCGCATGGAAGAGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCgg                                                                                          <  2:1799438/150‑1 (MQ=255)
                                                           gTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTg                                                                                     >  2:1359048/1‑150 (MQ=255)
                                                            gcGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGt                                                                                    <  2:1692376/149‑1 (MQ=255)
                                                              gCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCg                                                                                  <  1:2007426/150‑1 (MQ=255)
                                                              gCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCg                                                                                  >  1:720686/1‑150 (MQ=255)
                                                                 tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCa                                                                               <  1:1850842/150‑1 (MQ=255)
                                                                 tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCa                                                                               <  1:738200/150‑1 (MQ=255)
                                                                     agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATaaa                                                                           <  2:349284/150‑1 (MQ=255)
                                                                        ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATTTCCCGCGGATCTTCTGAAAGAATTATCCCGGTATTGTCGGCATTAAgct                                                                        >  1:1525780/1‑148 (MQ=255)
                                                                         gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATg                                                                       <  2:224508/150‑1 (MQ=255)
                                                                          tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATgg                                                                      <  2:1999410/150‑1 (MQ=255)
                                                                                 aCGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCgg                                                               <  1:1884281/150‑1 (MQ=255)
                                                                                  cGCTTCATCTTCCTCGTAATTTCGTTTCTGTGTAGGCCAACTGGTTACGCAGAAACAAGGCCCCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGga                                                              <  2:290737/150‑1 (MQ=255)
                                                                                   gCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGgag                                                             <  1:457782/150‑1 (MQ=255)
                                                                                    cTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGACGCCGgaga                                                            >  2:108734/1‑150 (MQ=255)
                                                                                       cATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGagaaga                                                         >  2:1048721/1‑150 (MQ=255)
                                                                                          cTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAgg                                                      <  1:582690/150‑1 (MQ=255)
                                                                                          cTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAgg                                                      <  1:655682/150‑1 (MQ=255)
                                                                                           ttCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGt                                                     <  1:1413383/150‑1 (MQ=255)
                                                                                            tCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTg                                                    <  1:921639/150‑1 (MQ=255)
                                                                                              cgcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGac                                                  >  2:735388/1‑150 (MQ=255)
                                                                                                 gTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCAGAAAGATGTTCGCCGGAGAAGAAGGTGCCAcc                                               >  2:1289278/1‑150 (MQ=255)
                                                                                                      ttCTTTACTGTGTAGGCCAACAGGAAACGCAGAAAAAAGGCACCTTGCGGTGACATTCTTATAATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTTTCGGCATAAACATTGTCGCCGGAGAAGAATGTGACAACGCCaa                                          >  2:1272781/1‑150 (MQ=255)
                                                                                                       tCTTTACTGTGTAGGCCAACAGGTAACGCAGAATAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCaaa                                         >  2:604266/1‑150 (MQ=255)
                                                                                                         tttACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAt                                       <  1:1735503/150‑1 (MQ=255)
                                                                                                          ttACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAtt                                      <  1:1641974/150‑1 (MQ=255)
                                                                                                          ttACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAtt                                      <  2:746835/150‑1 (MQ=255)
                                                                                                           tACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGTCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTg                                     <  1:1183045/150‑1 (MQ=255)
                                                                                                           tACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGCCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTg                                     <  1:1313766/150‑1 (MQ=255)
                                                                                                            aCTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAttta                                    >  2:1520259/1‑148 (MQ=255)
                                                                                                               gtgtAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCATATGAAAGAATAAACCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcgc                                 >  1:627192/1‑150 (MQ=255)
                                                                                                                tttAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCAGGTATTGTCGGCATAAATATGGTCGCCGGAGAATAAGGTGACACCGGCAAAATTGAcgcg                                >  2:298446/3‑150 (MQ=255)
                                                                                                                   aGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGAc                             <  1:1532571/150‑1 (MQ=255)
                                                                                                                    ggCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACa                            >  1:1922763/1‑150 (MQ=255)
                                                                                                                      ccAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATc                          >  1:613781/1‑150 (MQ=255)
                                                                                                                      ccAACAGGTAACGCAGAAAAAAGGCACCATGCGGAGCCTTTCTTATCATACAATATCCAGCGGATCTTCTGAAAGAAAAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACAACTCCAAAATTGACGCGGACATc                          >  2:1007534/1‑150 (MQ=255)
                                                                                                                             gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCTGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCg                   <  2:1207663/150‑1 (MQ=255)
                                                                                                                             gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCg                   >  1:692804/1‑150 (MQ=255)
                                                                                                                              tAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTTTCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTTTTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGc                  <  1:555232/150‑1 (MQ=255)
                                                                                                                               aaCGCAGTAAAAAGGCACGGTGCGGTTGCTTTCTTATCATTCAATATCCAGCGGATCTTATGAAATAATAATCCCGGTATTGTCGTCATACAGATGGTCGCCGGAGAAGATGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGcc                 <  1:96237/150‑1 (MQ=255)
                                                                                                                               aaCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGcc                 <  2:315260/150‑1 (MQ=255)
                                                                                                                               aaCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGGCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGcc                 <  1:1238817/150‑1 (MQ=255)
                                                                                                                                 cGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa               >  1:1561260/1‑150 (MQ=255)
                                                                                                                                 cGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa               >  2:1156382/1‑150 (MQ=255)
                                                                                                                                   cAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATg             <  2:631541/150‑1 (MQ=255)
                                                                                                                                    aGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGc            >  2:535235/1‑150 (MQ=255)
                                                                                                                                     gAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCAGGTATTTTCGGCATAAATATGGTCGCCTGAGAAGAAGATGACACCTCAAAAATTTACGCGGAAATCGCTTTCGCAAATTcc           >  1:1292585/1‑150 (MQ=255)
                                                                                                                                      aagaaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGGCAATGCCt          >  1:1293104/4‑150 (MQ=255)
                                                                                                                                        aaaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTc        <  1:548583/150‑1 (MQ=255)
                                                                                                                                         aaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGATTTCGCCAATGCCTTcc       >  2:1269309/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGC  >  NC_000913/4118682‑4118973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: