Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,176,408 T→C 15.3% R155R (CGT→CGC)  tufB → translation elongation factor EF‑Tu 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,176,4080TC15.3% 43.1 / 3.5 26R155R (CGT→CGC) tufBtranslation elongation factor EF‑Tu 2
Reads supporting (aligned to +/- strand):  ref base T (13/9);  new base C (2/2);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

TGACGGCCCGATGCCGCAGACTCGTGAGCACATCCTGCTGGGTCGTCAGGTAGGCGTTCCGTACATCATCGTGTTCCTGAACAAATGCGACATGGTTGATGACGAAGAGCTGCTGGAACTGGTTGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAACCAGAG  >  NC_000913/4176270‑4176555
                                                                                                                                          |                                                                                                                                                   
tgccGGCCCGATGCCGCAGACTCGGGTGCACAGCCTGCTGGGTCGTCAGGTAGGCGTTCCGTACATCATCGTGTTCCCGAACAAATCCGACATGTTTGATGACGAAGAGCTGCTGGAACTGGTTGAAATGGCAGTTCACGAACTTccatt                                                                                                                                          <  2:91689/147‑5 (MQ=1)
                                                                     cgggtTCCTGAACAAATGCGACATGGTTGAGGACGAAGAGCGGCTGGAACTGGTTGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGa                                                                     <  2:396503/147‑1 (MQ=0)
                                                                                                                         gTTGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTAc                 >  1:366079/1‑150 (MQ=11)
                                                                                                                          ttGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACa                >  1:992971/1‑150 (MQ=31)
                                                                                                                          ttGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACa                <  1:445828/150‑1 (MQ=31)
                                                                                                                          ttGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACa                >  1:251959/1‑150 (MQ=31)
                                                                                                                             aaaTGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTc             >  2:1304189/1‑150 (MQ=32)
                                                                                                                             aaaTGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTc             >  2:1281747/1‑150 (MQ=32)
                                                                                                                             aaaTGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTc             >  2:411617/1‑150 (MQ=32)
                                                                                                                              gacagAAGTTCACGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACAATCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTcc            >  2:73458/5‑150 (MQ=255)
                                                                                                                              aaTGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTcc            >  1:1011719/1‑150 (MQ=32)
                                                                                                                               aTGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGATAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAACTCCAGGCACTGGCTGGCTTCCTGGAGTCTTCCAATCCg           >  2:974865/1‑150 (MQ=11)
                                                                                                                                tGGACGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGGGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGGGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCgg          <  1:1999772/150‑1 (MQ=12)
                                                                                                                                cagAAGTTCACGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCgg          >  1:211433/3‑150 (MQ=12)
                                                                                                                                  gAAGTTCGTTAACTTCTGTATCAGAACGACTTCCCGGTCGCCGAAACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTTGAAGGCGACGCAGAGAGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGaa        <  2:493472/150‑1 (MQ=11)
                                                                                                                                  gAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGaa        <  2:935451/150‑1 (MQ=32)
                                                                                                                                  gAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGaa        <  1:939242/150‑1 (MQ=32)
                                                                                                                                   caGTTCGCGCACTTCTGGCTCAGTACGACTTCCCTGGCCCCGACACTCCGATCGATCGTGGTTCTGCGCTGAAAGCGCTGGAATGCGACGCAGAGTGGGAAGCGAAAATGCTCGAACTGGCTGGCTGCCTGGATTCTTACATTCCGGAAc       <  1:1295320/149‑1 (MQ=11)
                                                                                                                                   aaGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAAc       >  2:255096/1‑150 (MQ=32)
                                                                                                                                   aaGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAAc       <  1:198390/150‑1 (MQ=32)
                                                                                                                                    aGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCATAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAAcc      >  2:791557/1‑150 (MQ=17)
                                                                                                                                    aGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAAcc      <  1:523262/150‑1 (MQ=32)
                                                                                                                                     gTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCCGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAACCa     >  1:1426791/1‑150 (MQ=17)
                                                                                                                                      ttCGTGAACTTGTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAACCag    <  1:1890544/150‑1 (MQ=17)
                                                                                                                                        cGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAACCagag  >  1:1463934/1‑150 (MQ=32)
                                                                                                                                        cGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGAGGTTCTGCTCTGAAAGCGCTGGAAGTCCACGCAGAGTGGGAAGCGAAAATCCTGGAACTGCAAGGCTACCTGGATACTTACATTCCGGACCCagag  >  1:788813/1‑150 (MQ=11)
                                                                                                                                          |                                                                                                                                                   
TGACGGCCCGATGCCGCAGACTCGTGAGCACATCCTGCTGGGTCGTCAGGTAGGCGTTCCGTACATCATCGTGTTCCTGAACAAATGCGACATGGTTGATGACGAAGAGCTGCTGGAACTGGTTGAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAAGGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTACATTCCGGAACCAGAG  >  NC_000913/4176270‑4176555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑