Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,213,185 A→T 6.4% intergenic (+26/‑49) rrfE → / → yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,213,1850AT 6.4% 394.8 / 9.1 134intergenic (+26/‑49)rrfE/yjaA5S ribosomal RNA of rrnE operon/stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base A (61/64);  new base T (7/2);  total (68/66)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

ATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCA  >  NC_000913/4213037‑4213331
                                                                                                                                                    |                                                                                                                                                   
aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAg                                                                                                                                                    >  1:1547144/1‑150 (MQ=255)
aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACACCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAg                                                                                                                                                    >  1:1547145/1‑150 (MQ=255)
     ccTGGCTGCAGTCGCGCGGTGGGCCCACCTGACCCCATGCACAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGGGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                                                                              <  2:61235/150‑1 (MQ=17)
     ccTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                                                                              <  2:1745891/150‑1 (MQ=255)
           ggcAGTAGCGCGGGGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGCCTTATGGACCGGGGtttt                                                                                                                                        <  2:174678/150‑1 (MQ=14)
             cAGTAGCGCGGTGGTCCCACCTGTCCCCATGCCGAACTACGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGCCTTATGGACCGGGGtttttc                                                                                                                                      <  2:364206/150‑2 (MQ=14)
             cAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTATCGCCGATGGTAGTGGTGGGTCTCCCCATTCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGtttttc                                                                                                                                      <  1:351537/150‑2 (MQ=255)
             cAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGtttttt                                                                                                                                      >  1:1249989/1‑150 (MQ=255)
              aGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTg                                                                                                                                     <  1:1434992/150‑1 (MQ=255)
                tAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCa                                                                                                                                   <  2:53295/150‑1 (MQ=255)
                tAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGGGAAACGCCGTAGCGCCGCAGGTAGGGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCa                                                                                                                                   <  2:1059165/150‑1 (MQ=255)
                    gcgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATc                                                                                                                               <  1:1340695/150‑1 (MQ=255)
                         ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAatta                                                                                                                          <  2:1332407/150‑1 (MQ=255)
                         ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAatta                                                                                                                          >  1:708826/1‑150 (MQ=255)
                         ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAatta                                                                                                                          >  2:719692/1‑150 (MQ=255)
                         ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAatta                                                                                                                          >  1:1397179/1‑150 (MQ=255)
                         ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGGCTCCCCATGCGAGAGTAGGGTACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTCTTGCATATCAatta                                                                                                                          <  2:1544396/150‑1 (MQ=255)
                              cACCTGACCCCCTGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGc                                                                                                                     <  1:1954305/150‑1 (MQ=255)
                                 cTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatg                                                                                                                  <  1:1357118/150‑1 (MQ=255)
                                   gACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatgat                                                                                                                <  2:470164/150‑1 (MQ=255)
                                        cATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAgg                                                                                                           >  1:1643112/1‑150 (MQ=255)
                                              gAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAAtct                                                                                                     <  2:839017/150‑1 (MQ=255)
                                              gAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTATCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAAtct                                                                                                     >  1:627606/1‑150 (MQ=255)
                                                  tCAGAAGTGAAACGCCGTAGCGCCGATGGTACTGTGGGGTCTCCCCATGCTAGAGTTGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATTAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATg                                                                                                 <  2:991604/150‑1 (MQ=255)
                                                   cAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGt                                                                                                >  2:1577336/1‑150 (MQ=255)
                                                   cAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGt                                                                                                >  2:44535/1‑150 (MQ=255)
                                                   cAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGt                                                                                                >  1:1451753/1‑150 (MQ=255)
                                                    aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTc                                                                                               <  1:1487518/150‑1 (MQ=255)
                                                    aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGGGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTc                                                                                               <  2:1681905/150‑1 (MQ=255)
                                                     gAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCa                                                                                              >  2:72213/1‑150 (MQ=255)
                                                      aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAg                                                                                             >  2:271202/1‑150 (MQ=255)
                                                      aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAg                                                                                             <  2:254438/150‑1 (MQ=255)
                                                      aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAg                                                                                             <  2:539740/150‑1 (MQ=255)
                                                        gTGACACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGtt                                                                                           <  2:977824/150‑1 (MQ=255)
                                                        gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGtt                                                                                           >  1:477637/1‑150 (MQ=255)
                                                         ggAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTc                                                                                          <  2:1294650/149‑1 (MQ=255)
                                                            aaCGCCGAAGCTCCGATGCTAGTGTGGGGTCTCCCCTAGCGAGACAAGGGATCTGCCAGGCATCAAATTAGAAAAACCCCGGTCCAAAACGCCGGGGTTTTTTGCATATCAATTATATGCATCATGAACGGAATCTCATGTCAGTTCTGt                                                                                       <  2:1394371/150‑1 (MQ=255)
                                                            aaCGCAGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCTAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATTTCAATTATTTTCATGATGAAGGGAATCTCATGTCAGTTCTGt                                                                                       >  2:950752/1‑150 (MQ=255)
                                                             aCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGtt                                                                                      >  1:1694375/1‑149 (MQ=255)
                                                                ccGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAACCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTatat                                                                                   <  2:1572805/150‑1 (MQ=255)
                                                                ccGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTatat                                                                                   <  1:1691136/150‑1 (MQ=255)
                                                                ccGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTatat                                                                                   >  1:931201/1‑150 (MQ=255)
                                                                 cGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTTCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGTAGGGAATCTCATGTCAGTTCTGTATATc                                                                                  <  1:474116/150‑1 (MQ=255)
                                                                 cGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATc                                                                                  <  2:931201/150‑1 (MQ=255)
                                                                 cGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATc                                                                                  <  2:1958014/150‑1 (MQ=255)
                                                                       gcCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAAtt                                                                            >  2:157023/1‑150 (MQ=255)
                                                                       gcCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAAtt                                                                            >  2:751390/1‑150 (MQ=255)
                                                                        ccGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCAGATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtc                                                                           >  2:1592016/1‑150 (MQ=255)
                                                                           ttGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTTCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                                        <  1:1744394/149‑1 (MQ=255)
                                                                                    tgGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCaaa                                                               >  2:1134598/1‑150 (MQ=255)
                                                                                     ggggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCAGAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAt                                                              >  1:215834/1‑150 (MQ=255)
                                                                                          ctcCCCATGCGAGAGTAGGGAACTACCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTg                                                         >  2:1632449/1‑150 (MQ=255)
                                                                                            ccccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGtt                                                       >  2:168994/1‑150 (MQ=255)
                                                                                             cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTc                                                      <  2:1547145/150‑1 (MQ=255)
                                                                                             cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTc                                                      <  2:1547144/150‑1 (MQ=255)
                                                                                               cATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTcgc                                                    <  2:1730130/150‑1 (MQ=255)
                                                                                                 tGCGAGTGTAGGGAACTGCCAGGCTTCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGAGGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGa                                                  <  1:1832717/150‑1 (MQ=255)
                                                                                                   cGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATc                                                >  2:1922959/1‑150 (MQ=255)
                                                                                                    gagaGTAGGGTACTGCCAGGCATCAAATTAGAAAAACGACGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCt                                               <  1:1249266/150‑1 (MQ=255)
                                                                                                    gagaGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCt                                               >  2:641687/1‑150 (MQ=255)
                                                                                                     agagTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCtt                                              >  1:426157/1‑150 (MQ=255)
                                                                                                      gagTAGGGAACTGCCAGGCATCAAAATAGAAAAACCCCGGTCGATTAGGCCGGGGTTTTTTGCATATCTATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTg                                             <  2:14280/150‑1 (MQ=255)
                                                                                                        gTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaa                                           <  2:805100/150‑1 (MQ=255)
                                                                                                         tAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa                                          <  1:553454/150‑1 (MQ=255)
                                                                                                          aGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAg                                         >  1:1073047/1‑150 (MQ=255)
                                                                                                          aGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAg                                         <  1:883721/150‑1 (MQ=255)
                                                                                                           gggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTATTCAAATTACTGTTCGCGATCTTGAAAGc                                        >  2:1141605/1‑150 (MQ=255)
                                                                                                            ggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCa                                       >  1:770343/1‑150 (MQ=255)
                                                                                                             gAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCAGGGGATTATTGCATATCAATTATTTGCAAGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGATCCAGATCTTGAAAGCaa                                      >  1:308363/1‑150 (MQ=255)
                                                                                                                  gCCAGGCATCAAATTAGAAAAACCCAGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGTAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTg                                 <  2:458047/150‑1 (MQ=255)
                                                                                                                     aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTACTCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAg                              <  2:247661/150‑1 (MQ=255)
                                                                                                                     aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAg                              <  1:271202/150‑1 (MQ=255)
                                                                                                                       gCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgtg                            <  2:477637/150‑1 (MQ=255)
                                                                                                                          tCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCa                         >  1:1676305/1‑150 (MQ=255)
                                                                                                                           cAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAg                        <  2:1862054/150‑1 (MQ=255)
                                                                                                                            aaaTTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAgg                       <  1:1007695/150‑1 (MQ=255)
                                                                                                                             tatgcaaaaaaCCCCGGCCTTATGGACCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGga                      >  1:1889612/7‑150 (MQ=38)
                                                                                                                               ttAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGgaga                    <  1:611714/150‑1 (MQ=255)
                                                                                                                                gcaaaaaaCCCCGGCCTTATGGACCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGAc                   >  1:860556/4‑150 (MQ=255)
                                                                                                                                gcaaaaaaCCCCGGCCTTATGGACCGGGGTTTTTTGCATATCAAATATTTGCATGATGAAGGGAATCTCATTTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCATGAAAGCAAACGTGAAGTGTCAGGAGAc                   >  1:900681/4‑150 (MQ=255)
                                                                                                                                gcaaaaaaCCCCGGCCGTATGGACCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGTATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGATAc                   >  2:1646910/4‑150 (MQ=255)
                                                                                                                                 caaaaaaCCCCGGCCTTATGGACCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACg                  >  2:805010/3‑150 (MQ=255)
                                                                                                                                 caaaaaaCCCCGGCCTTATGGACCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTAAGGAGAcc                  >  2:1018861/3‑149 (MQ=255)
                                                                                                                                 aGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACg                  >  1:1417453/1‑150 (MQ=255)
                                                                                                                                 aGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACg                  >  1:1624840/1‑150 (MQ=255)
                                                                                                                                 aGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACg                  >  2:1216573/1‑150 (MQ=255)
                                                                                                                                 aGAAAAACCCAGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGCCg                  >  2:916536/1‑150 (MQ=255)
                                                                                                                                  gAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGc                 >  2:579186/1‑150 (MQ=255)
                                                                                                                                  gAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGc                 <  1:1081070/150‑1 (MQ=255)
                                                                                                                                  gAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGc                 >  1:1718806/1‑150 (MQ=255)
                                                                                                                                   aaaaaCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAGGGGAATCTCATGTCAGTTCTGTATATCCATATTCGTCGTAATGAAATTACTGTTCGCGATCTTGAAAGCATACGTGATGTGTCAGGAGACGCt                >  1:98124/1‑150 (MQ=255)
                                                                                                                                    aaaaCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTg               >  1:579159/1‑150 (MQ=255)
                                                                                                                                    aaaaCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTg               <  1:157023/150‑1 (MQ=255)
                                                                                                                                       aCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCt            >  1:1956619/1‑150 (MQ=255)
                                                                                                                                       aCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCt            <  2:1624840/150‑1 (MQ=255)
                                                                                                                                         cccgcgccATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTc          <  2:1274998/144‑1 (MQ=255)
                                                                                                                                           cGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAg        <  2:671507/150‑1 (MQ=255)
                                                                                                                                           aggTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAg        <  2:365463/149‑1 (MQ=255)
                                                                                                                                              tCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTaa     <  1:719692/150‑1 (MQ=255)
                                                                                                                                               ccATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAAc    >  1:511554/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCA  >  NC_000913/4213037‑4213331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: